Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. ...Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. In this study, SSR locus around homologous sequences of cold-tolerant genes w</span><span style="font-family:"">as</span><span style="font-family:""> searched and screened using MD-2 (cold-tolerant variety) and Tainong 17 (cold-sensitive variety). Seventy three pairs of primers of which PCR results were different between these two varieties were gotten. PCRs were performed using these as primers and genomic DNA of germplasms collected as templates. Six pairs of primers were found that their PCR results were in good consistent with cold tolerance. Using GLM (General linear model) association <span>mapping analysis, two SSR markers linked to cold tolerance were found. Their</span> efficiency was verified further using pineapple germplasms with high cold tolerance which had been validated in field by cold wave. They will be used in cold-tolerant breeding of pineapple in the future.展开更多
Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular sp...Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular space of translucent fruit than that of normal flesh. The contents of alcohol and ethylene in translucent fruit were higher than those in normal fruit. Translucent fruit contained less calcium than normal fruit. Electrolyte leakage of translucent flesh was more than that of normal flesh. There were 205 proteins of which the expressions in translucent flesh were higher than those in normal flesh. Calcium-ions-binding protein EF-hand domain-containing protein, ethylene-synthesizing enzyme 1-aminpcyclopropane-1-carboxylate oxidase, ROS-producing protein universal stress protein A-like protein were the top three proteins of which the expressions in translucent flesh were higher than those in normal fruit. When much sugar was transferred into fruit pulp and accumulated in intercellular space, water will be absorbed from cells around and translucence formed. The accumulation of sugar and liquid in apoplast were due to that cell wall and membrane were degraded, which was from being attacked by ROS. There might be more and larger pores in cell wall and membranes of translucent flesh. These data played foundations for researching methods for controlling pineapple translucency.展开更多
文摘Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. In this study, SSR locus around homologous sequences of cold-tolerant genes w</span><span style="font-family:"">as</span><span style="font-family:""> searched and screened using MD-2 (cold-tolerant variety) and Tainong 17 (cold-sensitive variety). Seventy three pairs of primers of which PCR results were different between these two varieties were gotten. PCRs were performed using these as primers and genomic DNA of germplasms collected as templates. Six pairs of primers were found that their PCR results were in good consistent with cold tolerance. Using GLM (General linear model) association <span>mapping analysis, two SSR markers linked to cold tolerance were found. Their</span> efficiency was verified further using pineapple germplasms with high cold tolerance which had been validated in field by cold wave. They will be used in cold-tolerant breeding of pineapple in the future.
文摘Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular space of translucent fruit than that of normal flesh. The contents of alcohol and ethylene in translucent fruit were higher than those in normal fruit. Translucent fruit contained less calcium than normal fruit. Electrolyte leakage of translucent flesh was more than that of normal flesh. There were 205 proteins of which the expressions in translucent flesh were higher than those in normal flesh. Calcium-ions-binding protein EF-hand domain-containing protein, ethylene-synthesizing enzyme 1-aminpcyclopropane-1-carboxylate oxidase, ROS-producing protein universal stress protein A-like protein were the top three proteins of which the expressions in translucent flesh were higher than those in normal fruit. When much sugar was transferred into fruit pulp and accumulated in intercellular space, water will be absorbed from cells around and translucence formed. The accumulation of sugar and liquid in apoplast were due to that cell wall and membrane were degraded, which was from being attacked by ROS. There might be more and larger pores in cell wall and membranes of translucent flesh. These data played foundations for researching methods for controlling pineapple translucency.
