Objective:Lymphatic endothelial cell(LEC)proliferation is essential for lymphangiogenesis.Hypoxia induces lymphangiogenesis,but it directly inhibits LEC proliferation and the underlying mechanisms have not been fully ...Objective:Lymphatic endothelial cell(LEC)proliferation is essential for lymphangiogenesis.Hypoxia induces lymphangiogenesis,but it directly inhibits LEC proliferation and the underlying mechanisms have not been fully understood.The aim of this study was to investigate the role of carcinoembryonic antigen-related cell adhesion molecule 1(CEACAM1)in hypoxia-repressed LEC proliferation.Methods:Human dermal lymphatic endothelial cells(HDLECs)were cultured under normoxic or hypoxic conditions,and cell proliferation was determined using MTT or CCK-8 assays.CEACAM1 expression was silenced by siRNA transfection.Activation of mitogen-activated protein kinases(MAPKs)was examined by Western blotting and blocked by specific inhibitors.Results:Under hypoxia,HDLECs proliferation was suppressed and CEACAM1 expression was downregulated.Silence of CEACAM1 in normoxia inhibited HDLECs proliferation and did not further decrease proliferation in HDLECs in response to hypoxia,suggesting that CEACAM1 may mediate hypoxia-induced inhibition of HDLECs proliferation.In addition,silence of CEACAM1 increased phosphorylation of MAPK molecules:extracellular signal-regulated kinase(ERK),p38 MAPK and Jun N-terminal kinase(JNK)in HDLECs.However,only inhibition of the JNK pathway rescued the reduction of HDLEC proliferation induced by CEACAM1 silence.Conclusion:Our results suggested that hypoxia downregulates CEACAM1 expression by activation of the JNK pathway,leading to inhibition of HDLEC proliferation.These findings may help to understand the mechanisms of LEC-specific response to hypoxia and develop novel therapies for pathological lymphangiogenesis.展开更多
将银耳蒂头制成超微粉,添加到制作面包原料中制成银耳蒂头面包。以感官评分为指标,研究银耳蒂头超微粉添加量、酵母添加量、水分添加量对银耳蒂头面包感官评分的影响。在单因素试验基础上,采用模糊数学综合评价法,通过Box-Benhnken响应...将银耳蒂头制成超微粉,添加到制作面包原料中制成银耳蒂头面包。以感官评分为指标,研究银耳蒂头超微粉添加量、酵母添加量、水分添加量对银耳蒂头面包感官评分的影响。在单因素试验基础上,采用模糊数学综合评价法,通过Box-Benhnken响应面法对银耳蒂头面包工艺配方进行优化。结果表明:银耳蒂头面包制作最佳制作工艺配方(以占高筋面粉百分比计算)为高筋面粉300g (100%)、银耳蒂头超微粉31.32g (10.44%)、细砂糖60g (20%)、黄油24g(8%)、酵母3.99 g (1.33%)、食盐1.8 g (0.8%)、鸡蛋液36 g (12%)、水138.39 g(46.13%)。采用该最佳制作工艺配方生产的银耳蒂头面包外表呈金黄色,外形饱满,表面光滑,具有面包特有的香味,手感柔软有弹性,口感柔软,不黏牙。展开更多
基金supported by grants from the National Natural Science Foundation of China(No.81873473 and No.91939110)Academic Promotion Program of Shandong First Medical University(No.2019QL014)Shandong Taishan Scholarship(Ju Liu).
文摘Objective:Lymphatic endothelial cell(LEC)proliferation is essential for lymphangiogenesis.Hypoxia induces lymphangiogenesis,but it directly inhibits LEC proliferation and the underlying mechanisms have not been fully understood.The aim of this study was to investigate the role of carcinoembryonic antigen-related cell adhesion molecule 1(CEACAM1)in hypoxia-repressed LEC proliferation.Methods:Human dermal lymphatic endothelial cells(HDLECs)were cultured under normoxic or hypoxic conditions,and cell proliferation was determined using MTT or CCK-8 assays.CEACAM1 expression was silenced by siRNA transfection.Activation of mitogen-activated protein kinases(MAPKs)was examined by Western blotting and blocked by specific inhibitors.Results:Under hypoxia,HDLECs proliferation was suppressed and CEACAM1 expression was downregulated.Silence of CEACAM1 in normoxia inhibited HDLECs proliferation and did not further decrease proliferation in HDLECs in response to hypoxia,suggesting that CEACAM1 may mediate hypoxia-induced inhibition of HDLECs proliferation.In addition,silence of CEACAM1 increased phosphorylation of MAPK molecules:extracellular signal-regulated kinase(ERK),p38 MAPK and Jun N-terminal kinase(JNK)in HDLECs.However,only inhibition of the JNK pathway rescued the reduction of HDLEC proliferation induced by CEACAM1 silence.Conclusion:Our results suggested that hypoxia downregulates CEACAM1 expression by activation of the JNK pathway,leading to inhibition of HDLEC proliferation.These findings may help to understand the mechanisms of LEC-specific response to hypoxia and develop novel therapies for pathological lymphangiogenesis.
文摘将银耳蒂头制成超微粉,添加到制作面包原料中制成银耳蒂头面包。以感官评分为指标,研究银耳蒂头超微粉添加量、酵母添加量、水分添加量对银耳蒂头面包感官评分的影响。在单因素试验基础上,采用模糊数学综合评价法,通过Box-Benhnken响应面法对银耳蒂头面包工艺配方进行优化。结果表明:银耳蒂头面包制作最佳制作工艺配方(以占高筋面粉百分比计算)为高筋面粉300g (100%)、银耳蒂头超微粉31.32g (10.44%)、细砂糖60g (20%)、黄油24g(8%)、酵母3.99 g (1.33%)、食盐1.8 g (0.8%)、鸡蛋液36 g (12%)、水138.39 g(46.13%)。采用该最佳制作工艺配方生产的银耳蒂头面包外表呈金黄色,外形饱满,表面光滑,具有面包特有的香味,手感柔软有弹性,口感柔软,不黏牙。
