Shenqi Fuzheng injection is extracted from the Chinese herbs Radix Astragali and Radix Codonopsis. The aim of the present study was to investigate the neuroprotective effects of Shenqi Fuzheng injection in cerebral is...Shenqi Fuzheng injection is extracted from the Chinese herbs Radix Astragali and Radix Codonopsis. The aim of the present study was to investigate the neuroprotective effects of Shenqi Fuzheng injection in cerebral ischemia and reperfusion. Aged rats（20–22 months） were divided into three groups： sham, model, and treatment. Shenqi Fuzheng injection or saline（40 m L/kg） was injected into the tail vein daily for 1 week, after which a cerebral ischemia/reperfusion injury model was established. Compared with model rats that received saline, rats in the treatment group had smaller infarct volumes, lower brain water and malondialdehyde content, lower brain Ca2＋ levels, lower activities of serum lactate dehydrogenase and creatine kinase, and higher superoxide dismutase activity. In addition, the treatment group showed less damage to the brain tissue ultrastructure and better neurological function. Our findings indicate that Shenqi Fuzheng injection exerts neuroprotective effects in aged rats with cerebral ischemia/reperfusion injury, and that the underlying mechanism relies on oxygen free radical scavenging and inhibition of brain Ca2＋ accumulation.展开更多
To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed d...To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed during the key period of female gametophyte abortion in mutant clones. The change of ABA content showed a similar pattern between normal and mutant clones, but the ABA content of the mutant clone was always much higher than that of the normal clone. In the normal clone, the IAA content decreased significantly at the early stage of the period and reached its minimum during the first ten days of April and stabilized thereafter. The endogenous ZR levels increased sharply at the early stages and reached a maximum in the middle of April, then decreased dramatically. The IAA and ZR contents of the mutant clone did not change perceptively through the entire period. We conclude that the high levels of ABA and the deficit of IAA and ZR may be the reasons of female gametophyte abortion in the female-sterile clones of P. tabulaeformis.展开更多
BACKGROUND Postpartum depression refers to a depressive episode or depressive symptoms up to 12 mo after delivery.Trait mindfulness has presented a protective factor for postpartum depressive symptoms and proved effic...BACKGROUND Postpartum depression refers to a depressive episode or depressive symptoms up to 12 mo after delivery.Trait mindfulness has presented a protective factor for postpartum depressive symptoms and proved efficient in improving relationship satisfaction among couples.AIM To investigate the correlations among mindfulness,marital quality,anxiety,and depression in a large city in western China during the post-corona virus infectious disease-2019 era and determine whether trait mindfulness mediates the relationship between marital quality and postpartum anxiety and depression among primiparas.METHODS A cross-sectional study was conducted.The self-administered questionnaire was submitted online through smartphones.The levels of mindfulness,anxiety,depression,and marital quality were respectively investigated by the mindful attention awareness scale(MAAS),the self-rating anxiety scale(SAS),the selfrating depression scale(SDS),and the marriage perception scale(MPS)in these enrolled Han and Tujia primiparas.RESULTS No statistical significance was observed in the prevalence of postpartum anxiety and depression,nor scores of MAAS and MPS-Total in different regions or ethnicities(P>0.05).However,MPSMarital interaction(P<0.05),MPS-Family relationship(MPS-FR)(P<0.01),and MPS-Marital conflict(MPS-MC)(P<0.01)scores of urban primiparas were higher than those of rural primiparas.The MPS-MC score of Han primiparas was higher than that of Tujia primiparas(P<0.05).Negative correlations were observed between MAAS and SAS(r=-0.457,P<0.01),and MAAS and SDS(r=-0.439,P<0.01).SAS has revealed a highly positive correlation with SDS(r=0.720,P<0.01)and a weak negative correlation with MPS(r=-0.200,P<0.05).Besides,a weak negative correlation was observed between MAAS and MPS-MC(r=-0.184,P<0.05),and a weak positive correlation was noticed between SAS and MPS-MC(r=-0.225,P<0.01).Mediation analysis demonstrated a full mediation effect of mindfulness level on the relationship between MPS-FR and postpartum anxiety(P<0.05,95%CI:-0.384 to 0.0展开更多
Objective:This study aims to explore the mechanism of Chinese medicine Zizhu substance in the ranging of diabetic foot ulcer model from the perspective of inflammatory response.Methods:High-fat feed and streptozotocin...Objective:This study aims to explore the mechanism of Chinese medicine Zizhu substance in the ranging of diabetic foot ulcer model from the perspective of inflammatory response.Methods:High-fat feed and streptozotocin(STZ)were 2 to establish diabetes,And an experimental model of diabetic foot ulcer was established with constant temperature and constant pressure.Scald The intervention control of The experimental model that included physiologicalEnzyme-linked immunosorbent assay(ELISA)was used to detect serum nitric oxide NO,C-reactive protein(CRP)and interleukin-6(il-6),Western blot(WB)detection of nuclear factor-bp65(NF-B P65),c-jun n-terminal kinase(JNK),Phosphorylated phosatidylinositol 3-kinase(p-pi3k),Immunohistochemistry(IHC)detection of vascular endothelial growth factor(VEGF)and leukemic cell leuk-34(CD34)of wound tissues,A cold atomic absorption method to detect blood mercury(Hg)content,study purple The effect of Zhu substance on The inflammation of diabetic foot ulcers and The safety of drugs.Results:The expression of inflammatory factors in diabetic foot ulcer rats was who that in ordinary ulcers,Zizhu substance could reduce The expression of inflammatory factors(NO,CRP and IL-6)in diabetic foot ulcer rats.In the diabetic foot ulcer model,the activation of the nf-kappa B,JNK,PI3K pathway,Zizhu substance can significantly inhibit the phosphorylation of JNK and nf-kappa B P65,PI3K,reduce the inflammation of the wound,happens the expression of VEGF,CD34 and promote the wound Angiogenesis and the detection of blood mercury content in rats treated with Zizhu lought indicate that the drug has good safety.Conclusion:Zizhu substance can promote diabetic foot ulcer tissue repair and improve wound ranging by inhibiting inflammation-related pathways,Showing that Zizhu substance is a safe and effective drug for the treatment of diabetic foot ulcers.展开更多
Objective:To review the systematic reviews of acupuncture for diabetic peripheral neuropathy(DPN)and to provide evidence for clinical decisions.Methods:Published systematic reviews targeting acupuncture treatment of D...Objective:To review the systematic reviews of acupuncture for diabetic peripheral neuropathy(DPN)and to provide evidence for clinical decisions.Methods:Published systematic reviews targeting acupuncture treatment of DPN were searched using computer through both Chinese and English databases till July 1,2019.Two researchers screened the papers based on inclusion and exclusion criteria and conducted report quality evaluation,methodological quality assessment and evidence quality grading using the preferred reporting items for systematic reviews and meta-analyses(PRISMA),assessment of multiple systematic review 2(AMSTAR 2)and grading of recommendations assessment,development and evaluation(GRADE).Results:Ten systematic reviews were included,involving 11 outcome measures.According to PRISMA,6 items were sufficiently reported while 1 item was not;AMSTAR 2 appraised that all the included systematic reviews were of low quality in the methodological evaluation;according to GRADE,of the 30 clinical evidences,only 5 were graded moderate while the remained were graded low or extremely low.Descriptive analysis showed that acupuncture can significantly improve DPN symptoms,accelerate the conduction velocities of sensory and motor nerves,and up-regulate the content of plasma nitric oxide(NO),while the adverse reaction rate was low.Conclusion:Acupuncture can produce satisfactory clinical efficacy in treating DPN,but the existing problems,such as low-quality evidence,unitary outcome measures,poor methodological quality of systematic reviews and nonstandard reporting,need to be treated cautiously;meanwhile,more high-quality clinical trials are required to elevate the level of evidence.展开更多
Objective:Based on data mining,to explore the clinical characteristics of traditional Chinese medicine ointment in the treatment of diabetic foot ulcer.Methods:to search the literatures about the treatment of diabetic...Objective:Based on data mining,to explore the clinical characteristics of traditional Chinese medicine ointment in the treatment of diabetic foot ulcer.Methods:to search the literatures about the treatment of diabetic foot ulcer by traditional Chinese medicine ointment in CNKI,Wangfang and VIP.In this paper,the prescription of traditional Chinese medicine ointment in the literature of treating diabetic foot ulcer was extracted,and the database of prescription was established by using v2.5 to analyze and mine the rule of medication.Results:95 prescriptions for diabetic foot ulcer were sorted out,involving 158 prescriptions.Among them,dragon's blood and Lithospermum are the most frequently used drug combinations.The core combination of 10 groups and 5 new prescriptions are obtained by evolution.Conclusion:the treatment of diabetic foot ulcer with traditional Chinese medicine ointment mainly includes clearing away heat,promoting blood circulation and removing blood stasis,and tonifying deficiency.展开更多
Objective:To to investigate the correlation between the level of immune function and diabetic foot in type 2 diabetic patients.Methods:76 diabetic patients in our hospital from January 2017 to November 2019 were colle...Objective:To to investigate the correlation between the level of immune function and diabetic foot in type 2 diabetic patients.Methods:76 diabetic patients in our hospital from January 2017 to November 2019 were collected as the observation group(the observation group was divided into two subgroups:38 cases in DM group and 38 cases in DM+DF group)and 76 healthy people in the same period as the healthy control group.The level of cellular immunity in serum of the observation group and the healthy control group were compared,and the relationship between cellular immunity and diabetic foot was further explored Department.Results:there were significant differences in glycosylated hemoglobin and fasting blood glucose between the two groups(P<0.001);there were significant differences in CD8,CD25 and CD28 between the two groups(P<0.001).There were statistical differences in the course and age of diabetes between the two subgroups in the observation group(P<0.05);there were statistical differences in CD2,CD8,CD25 and CD28 between the two subgroups(P<0.05).Logistic regression analysis showed that CD8[or95%CI 0.920(0.850,0.995)]and CD25[or95%CI 0.764(0.619,0.943)]were independent influencing factors and risk factors of diabetic foot.Conclusion:the increased levels of CD8 and CD25 may increase the risk of diabetic foot.展开更多
Objective: To observe the effect of Zizhu ointment on acute wound healing and its effect on the expression of autophagy proteins LC3 and Becin1. Method: SD rat model with acute skin injury were randomly divided into g...Objective: To observe the effect of Zizhu ointment on acute wound healing and its effect on the expression of autophagy proteins LC3 and Becin1. Method: SD rat model with acute skin injury were randomly divided into groups. The experimental group was treated with external application of Zizhu ointment, and the control group was given external saline. The wound healing rate of the two groups was compared at 4d, 7d, 14d and 21d after trauma. The morphology of wounds was observed by HE staining, and the autophagosomes of wounds were observed by transmission electron microscopy. Expression and changes of autophagy marker proteins Beclin-1 and LC3 were detected by immunohistochemistry and WB assay. Result: Compared with the control group, experimental group had a high healing rate, a short healing time, and a faster epithelialization rate. Under the electron microscope, autophagosomes of experimental group were reduced, and the expression of LC3 and Beclin-1 was significantly decreased. Conclusion: Zizhu ointment may promote wound healing by inhibiting autophagy activity of cells.展开更多
Primary erythema limb pain is a very rare disease,and its pathogenesis is not clear.Usually,the pain symptoms of patients exist for a long time,which seriously affect the daily life of patients and increase the econom...Primary erythema limb pain is a very rare disease,and its pathogenesis is not clear.Usually,the pain symptoms of patients exist for a long time,which seriously affect the daily life of patients and increase the economic burden of patients.Professor Liu Guobin,who has been engaged in the diagnosis and treatment of peripheral vascular diseases for nearly 30 years,believes that heat-toxin and blood stasis collaterals are closely related to the disease,and emphasizes the principle of heat-toxin and evil qi causing the disease.It is emphasized that clearing heat and detoxification,promoting blood circulation and dispersing blood stasis are the main treatment principles.This paper selects a typical case to describe Professor Liu's clinical experience in the treatment of primary erythema limb pain by oral and external use of traditional Chinese medicine,so as to provide an effective method of traditional Chinese medicine for the treatment of primary erythema limb pain.展开更多
Venous ulcer of lower extremities is a serious manifestation of venous insufficiency of lower extremities in the later stage,which is characterized by inflammatory exudation at the initial stage,followed by skin ruptu...Venous ulcer of lower extremities is a serious manifestation of venous insufficiency of lower extremities in the later stage,which is characterized by inflammatory exudation at the initial stage,followed by skin rupture,and finally forms the whole layer of skin atrophy and necrosis,which does not heal for a long time.How to promote ulcer healing is the most important problem in the treatment of venous ulcers of lower extremities.Vacuum sealing drainage(VSD)is constantly used in clinic and plays a unique advantage in wound healing.This paper mainly reviews the present situation of venous ulcer of lower extremities and summary,principle,application progress,attentions and prospect of VSD at home and abroad.展开更多
Objective:To explore the relationship between the changes of serum vitamin level in patients with type 2 diabetes mellitus and diabetic foot.Method Totally 68 patients with type 2 diabetes admitted to our hospital fro...Objective:To explore the relationship between the changes of serum vitamin level in patients with type 2 diabetes mellitus and diabetic foot.Method Totally 68 patients with type 2 diabetes admitted to our hospital from September 2018 to March 2019 were selected as observation group(DM group:Totally 32 patients without diabetic foot group,DM+DF group of 34 patients)and 68 patients with physical examination in our hospital(healthy control group)at the same time,and the serum vitamin levels of the two groups of patients were compared.On this basis,the relationship between serum vitamin level and diabetic foot was further explored.Results There were differences between the observation group and the healthy control group in the history of hypertension,2h postprandial blood glucose and glycosylated hemoglobin between the two groups(all P<0.001).Compared with the vitamin levels of the observation group and the healthy control group,there were statistical differences in vitamin B6 and vitamin B1 and vitamin B9 and Vitamin C and vitamin D(P<0.001),but there were no differences in vitamin A,vitamin B2 and vitamin E(P>0.05).Compared with the vitamin levels in the two subgroups of the observation group,there were statistical differences in vitamin B6 and vitamin B1 and vitamin B9 and Vitamin C(P<0.001).Logistic regression analysis showed that vitamin B6[OR95%CI 0.633(0.434,0.924)],vitamin B1[OR95%CI 0.705(0.524,0.949),vitamin B9[OR95%CI 0.248(0.063,0.978),and Vitamin C[OR95%CI 0.584(0.409,0.835)were risk factors for diabetic foot.Conclusion Vitamin B6,vitamin B1,vitamin B9 and Vitamin C can be used as indicators to predict the risk of DF in T2DM patients.展开更多
Objective: To analyze the advantages of Traditional Chinese Cutting Therapy at the present stage and the characteristics of clinical research and application, so as to provide a basis for clinical treatment and resear...Objective: To analyze the advantages of Traditional Chinese Cutting Therapy at the present stage and the characteristics of clinical research and application, so as to provide a basis for clinical treatment and research. Methods: Search the Chinese and English databases of relevant literatures on Traditional Chinese Cutting Therapy, and make statistics and Analysis on the distribution, types, distribution, indications and dominant diseases, the number of cases, clinical efficiency, intervention measures and operation sites of the published literatures. Results: A total of 311 articles were included. There are 40 indications for Traditional Chinese Cutting Therapy at this stage. The dominant diseases are psoriasis, asthma, facial paralysis, rhinitis, neurodermatitis, facial neuritis, chloasma, acne, migraine and insomnia. Traditional Chinese Cutting Therapy is closely related to meridian theory. At present, there are few theoretical and basic studies, the quality of relevant clinical studies is low, and the writing standard of research reports is inadequate. Conclusion: At present, the indications of Traditional Chinese Cutting Therapy are extensive, and the dominant diseases are concentrated, with dermatological diseases and neurological diseases as the main diseases. In the future, we should increase theoretical research and basic research, attach importance to the combination of meridian theory and Traditional Chinese Cutting Therapy, and standardize the implementation of clinical research and the writing of reports.展开更多
Objective: To overview the systematic reviews of acupuncture for Post-stroke Dysphagia. Methods: The Chinese and English databases were searched for the systematic reviews of dysphagia after acupuncture treatment. The...Objective: To overview the systematic reviews of acupuncture for Post-stroke Dysphagia. Methods: The Chinese and English databases were searched for the systematic reviews of dysphagia after acupuncture treatment. The retrieval time was until October 30, 2019. The final literature was evaluated for bias risk, methodology quality and evidence quality by using ROBIS tool, AMSTER-2 scale and GRADE method. Results: 9 systematic reviews and 36 outcomes were included. ROBIS bias risk assessment results show that all the studies are high bias risk;AMSTER-2 methodology quality assessment results show that all the systems are of very low quality;GRADE grading shows that there are only 7 intermediate evidences in 36 clinical evidences, the rest are low-level evidences or very low-level evidences, and there are multiple intermediate evidences in the clinical efficacy of acupuncture in the treatment of dysphagia after stroke. In addition, acupuncture can significantly improve the scores of all kinds of swallowing function related scales, such as Tengdao food intake swallowing function grade, swallowing disorder specific quality of life score, standard swallowing function evaluation scale, and the incidence of adverse reactions is low. Conclusion: Acupuncture is effective in the treatment of dysphagia after stroke. It can improve the scores of various swallowing function scales and has high safety. However, the risk of systematic evaluation bias is high, the quality of methodology is low, and the level of clinical evidence is low as a whole. In the future, the relevant research design should be more rigorous, and the research report should be written in strict accordance with the PRISMA statement.展开更多
Objective: It is expected to establish a diabetic ulcer rat model infected with Escherichia coli to provide an appropriate model for the experimental study of diabetic foot ulcer. Method: 32 SD male rats were randomly...Objective: It is expected to establish a diabetic ulcer rat model infected with Escherichia coli to provide an appropriate model for the experimental study of diabetic foot ulcer. Method: 32 SD male rats were randomly divided into three groups including the common ulcer group (N), the diabetic ulcer group (DU) and the model group (M) and Befuji group (B). The stability and practicability of the model were evaluated by HE performed on the local skin tissue of the wounds and ELISA detection detecting the content of High sensitive c-reactive protein (HCRP), basic fibroblast growth factor (bFGF) in rat serum after modeling and HCRP, bFGF, tumor necrosis factor-alpha (TNF-α), advanced glycation end products (AGEs), Interleukin-1β (IL-1β) and Interleukin-6 (IL-6) levels after wound healing. Results: After modeling, the HCRP content in N group was lower than that in the other three groups. There was no significant difference in bFGF content between the groups. After wound healing, the HCRP content in serum of rats in each group showed statistical difference. The level of HCRP in group B was lower than that in group M. There was no difference in bFGF content between the groups. The level of bFGF in group B was higher than that in group N. The contents of AGEs in each group were statistically different. The contents of AGEs in group B were lower than those in group DU and group M. The contents of TNF-α, IL-1βand IL-6 in each group were different. The levels of TNF-α, IL-1βand IL-6 in group B were lower than those in group M. Compared with DU group, the levels of TNF-α, IL-1βin M group were higher . Wound healing time in group M was significantly delayed than that in group N, group DU and group B. Conclusion:This model is more in line with the local environmental characteristics of infected diabetic ulcer and provides a reliable tool for the study of diabetic infections.展开更多
Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats...Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats were randomly divided into five groups: general ulcer group, diabetic ulcer group, diabetic ulcer treatment group, model group, and model treatment group. After modeling, take local skin tissue for HE staining.Detection of high-sensitivity C-reactive protein, fibroblast growth factor, tumor necrosis factor-α, advanced glycosylation products, interleukin-1β, interleukin-6, and nitric oxide in rat serum by enzyme-linked immunosorbent Level, and record the wound healing time, and evaluate the stability and practicability of the model. Results:The healing time of ulcer in the model group was significantly delayed compared with other groups (p <0.001). The serum H-CRP levels in the model group and the model treatment group were higher than those in the common ulcer group, the diabetic ulcer group, and the diabetic ulcer treatment group (p <0.05). After healing, the levels of H-CRP, bFGF, AGEs, TNF-α, IL-1β, IL-6, and NO in the serum of the diabetic ulcer group were higher than those in the common ulcer group (all p <0.05);The levels of TNF-α, IL-1β, IL-6, and NO were higher than those in other groups (all p <0.05);HCRP, AGEs, TNF-α, IL-1β, IL-6, and NO levels in the serum of the model treatment group They were all higher than those in diabetic ulcer treatment group (all p <0.05). Conclusion: This model is in line with the local environment of infectious diabetic ulcers and provides a reliable tool for the study of diabetic infections.展开更多
Venous hypertension is a potential mechanism for the formation of venous leg ulcers.Improving venous hypertension has become a key point in the treatment of venous leg ulcers.compression therapy is non-invasive and sa...Venous hypertension is a potential mechanism for the formation of venous leg ulcers.Improving venous hypertension has become a key point in the treatment of venous leg ulcers.compression therapy is non-invasive and safe and has become the most basic method for the treatment of venous leg ulcers and runs through the whole treatment process.This article summarizes the preparation before pressure treatment,matters needing attention in use and different types of pressure devices,so as to provide safe and effective treatment schemes for the vast number of doctors.展开更多
Diabetic foot is one of the serious complications of diabetes, which formed foot ulcer in the later stage of diabetes mellitus. The treatment of diabetic foot ulcer is difficult, greatly increasing the risk of amputat...Diabetic foot is one of the serious complications of diabetes, which formed foot ulcer in the later stage of diabetes mellitus. The treatment of diabetic foot ulcer is difficult, greatly increasing the risk of amputation, which will seriously affect the quality of life of patients. Promoting the healing of ulcer in the early stage has become the guiding ideology for the treatment of diabetic foot ulcer. Macrophages, as important immune cells, play a regulatory role throughout the healing process of diabetic foot ulcers. Therefore, we can provide a new direction for the treatment of diabetic foot ulcers by deeply understanding the mechanism of macrophages and the healing of diabetic foot ulcers.展开更多
基金supported by a grant from Key Technology Research and Development Program of Shaanxi Province of China,No.2003K10-G86the National Natural Science Foundation of China,No.30070731
文摘Shenqi Fuzheng injection is extracted from the Chinese herbs Radix Astragali and Radix Codonopsis. The aim of the present study was to investigate the neuroprotective effects of Shenqi Fuzheng injection in cerebral ischemia and reperfusion. Aged rats（20–22 months） were divided into three groups： sham, model, and treatment. Shenqi Fuzheng injection or saline（40 m L/kg） was injected into the tail vein daily for 1 week, after which a cerebral ischemia/reperfusion injury model was established. Compared with model rats that received saline, rats in the treatment group had smaller infarct volumes, lower brain water and malondialdehyde content, lower brain Ca2＋ levels, lower activities of serum lactate dehydrogenase and creatine kinase, and higher superoxide dismutase activity. In addition, the treatment group showed less damage to the brain tissue ultrastructure and better neurological function. Our findings indicate that Shenqi Fuzheng injection exerts neuroprotective effects in aged rats with cerebral ischemia/reperfusion injury, and that the underlying mechanism relies on oxygen free radical scavenging and inhibition of brain Ca2＋ accumulation.
基金Supported by the National Natural Science Foundation of China (Grant No. 30371144) and Postgraduate Foundation of Beijing Forestry University
文摘To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed during the key period of female gametophyte abortion in mutant clones. The change of ABA content showed a similar pattern between normal and mutant clones, but the ABA content of the mutant clone was always much higher than that of the normal clone. In the normal clone, the IAA content decreased significantly at the early stage of the period and reached its minimum during the first ten days of April and stabilized thereafter. The endogenous ZR levels increased sharply at the early stages and reached a maximum in the middle of April, then decreased dramatically. The IAA and ZR contents of the mutant clone did not change perceptively through the entire period. We conclude that the high levels of ABA and the deficit of IAA and ZR may be the reasons of female gametophyte abortion in the female-sterile clones of P. tabulaeformis.
基金Supported by Chongqing Science and Health Joint Medical Research Project,No.2021MSXM034.
文摘BACKGROUND Postpartum depression refers to a depressive episode or depressive symptoms up to 12 mo after delivery.Trait mindfulness has presented a protective factor for postpartum depressive symptoms and proved efficient in improving relationship satisfaction among couples.AIM To investigate the correlations among mindfulness,marital quality,anxiety,and depression in a large city in western China during the post-corona virus infectious disease-2019 era and determine whether trait mindfulness mediates the relationship between marital quality and postpartum anxiety and depression among primiparas.METHODS A cross-sectional study was conducted.The self-administered questionnaire was submitted online through smartphones.The levels of mindfulness,anxiety,depression,and marital quality were respectively investigated by the mindful attention awareness scale(MAAS),the self-rating anxiety scale(SAS),the selfrating depression scale(SDS),and the marriage perception scale(MPS)in these enrolled Han and Tujia primiparas.RESULTS No statistical significance was observed in the prevalence of postpartum anxiety and depression,nor scores of MAAS and MPS-Total in different regions or ethnicities(P>0.05).However,MPSMarital interaction(P<0.05),MPS-Family relationship(MPS-FR)(P<0.01),and MPS-Marital conflict(MPS-MC)(P<0.01)scores of urban primiparas were higher than those of rural primiparas.The MPS-MC score of Han primiparas was higher than that of Tujia primiparas(P<0.05).Negative correlations were observed between MAAS and SAS(r=-0.457,P<0.01),and MAAS and SDS(r=-0.439,P<0.01).SAS has revealed a highly positive correlation with SDS(r=0.720,P<0.01)and a weak negative correlation with MPS(r=-0.200,P<0.05).Besides,a weak negative correlation was observed between MAAS and MPS-MC(r=-0.184,P<0.05),and a weak positive correlation was noticed between SAS and MPS-MC(r=-0.225,P<0.01).Mediation analysis demonstrated a full mediation effect of mindfulness level on the relationship between MPS-FR and postpartum anxiety(P<0.05,95%CI:-0.384 to 0.0
基金National Natural Science Foundation of China Youth Program(No.81804095,81804095)Project of Collaborative Innovation Center for Traditional Chinese Medicine Health Services in Shanghai(No.ZYJKFW201701002)
文摘Objective:This study aims to explore the mechanism of Chinese medicine Zizhu substance in the ranging of diabetic foot ulcer model from the perspective of inflammatory response.Methods:High-fat feed and streptozotocin(STZ)were 2 to establish diabetes,And an experimental model of diabetic foot ulcer was established with constant temperature and constant pressure.Scald The intervention control of The experimental model that included physiologicalEnzyme-linked immunosorbent assay(ELISA)was used to detect serum nitric oxide NO,C-reactive protein(CRP)and interleukin-6(il-6),Western blot(WB)detection of nuclear factor-bp65(NF-B P65),c-jun n-terminal kinase(JNK),Phosphorylated phosatidylinositol 3-kinase(p-pi3k),Immunohistochemistry(IHC)detection of vascular endothelial growth factor(VEGF)and leukemic cell leuk-34(CD34)of wound tissues,A cold atomic absorption method to detect blood mercury(Hg)content,study purple The effect of Zhu substance on The inflammation of diabetic foot ulcers and The safety of drugs.Results:The expression of inflammatory factors in diabetic foot ulcer rats was who that in ordinary ulcers,Zizhu substance could reduce The expression of inflammatory factors(NO,CRP and IL-6)in diabetic foot ulcer rats.In the diabetic foot ulcer model,the activation of the nf-kappa B,JNK,PI3K pathway,Zizhu substance can significantly inhibit the phosphorylation of JNK and nf-kappa B P65,PI3K,reduce the inflammation of the wound,happens the expression of VEGF,CD34 and promote the wound Angiogenesis and the detection of blood mercury content in rats treated with Zizhu lought indicate that the drug has good safety.Conclusion:Zizhu substance can promote diabetic foot ulcer tissue repair and improve wound ranging by inhibiting inflammation-related pathways,Showing that Zizhu substance is a safe and effective drug for the treatment of diabetic foot ulcers.
文摘Objective:To review the systematic reviews of acupuncture for diabetic peripheral neuropathy(DPN)and to provide evidence for clinical decisions.Methods:Published systematic reviews targeting acupuncture treatment of DPN were searched using computer through both Chinese and English databases till July 1,2019.Two researchers screened the papers based on inclusion and exclusion criteria and conducted report quality evaluation,methodological quality assessment and evidence quality grading using the preferred reporting items for systematic reviews and meta-analyses(PRISMA),assessment of multiple systematic review 2(AMSTAR 2)and grading of recommendations assessment,development and evaluation(GRADE).Results:Ten systematic reviews were included,involving 11 outcome measures.According to PRISMA,6 items were sufficiently reported while 1 item was not;AMSTAR 2 appraised that all the included systematic reviews were of low quality in the methodological evaluation;according to GRADE,of the 30 clinical evidences,only 5 were graded moderate while the remained were graded low or extremely low.Descriptive analysis showed that acupuncture can significantly improve DPN symptoms,accelerate the conduction velocities of sensory and motor nerves,and up-regulate the content of plasma nitric oxide(NO),while the adverse reaction rate was low.Conclusion:Acupuncture can produce satisfactory clinical efficacy in treating DPN,but the existing problems,such as low-quality evidence,unitary outcome measures,poor methodological quality of systematic reviews and nonstandard reporting,need to be treated cautiously;meanwhile,more high-quality clinical trials are required to elevate the level of evidence.
基金General program of National nature science foundation(No.81774310)National nature science foundation for youth(No.81804095)Project for healthcare collaborative innovation center of TCM in Shanghai(No.ZYJKFW201701002)。
文摘Objective:Based on data mining,to explore the clinical characteristics of traditional Chinese medicine ointment in the treatment of diabetic foot ulcer.Methods:to search the literatures about the treatment of diabetic foot ulcer by traditional Chinese medicine ointment in CNKI,Wangfang and VIP.In this paper,the prescription of traditional Chinese medicine ointment in the literature of treating diabetic foot ulcer was extracted,and the database of prescription was established by using v2.5 to analyze and mine the rule of medication.Results:95 prescriptions for diabetic foot ulcer were sorted out,involving 158 prescriptions.Among them,dragon's blood and Lithospermum are the most frequently used drug combinations.The core combination of 10 groups and 5 new prescriptions are obtained by evolution.Conclusion:the treatment of diabetic foot ulcer with traditional Chinese medicine ointment mainly includes clearing away heat,promoting blood circulation and removing blood stasis,and tonifying deficiency.
基金NSFC(No.81774310)the Youth Project of National Natural Science Foundation(No.81804095)Shanghai TCM Health Service Collaborative Innovation Center Project(No.ZYJKFW201701002)
文摘Objective:To to investigate the correlation between the level of immune function and diabetic foot in type 2 diabetic patients.Methods:76 diabetic patients in our hospital from January 2017 to November 2019 were collected as the observation group(the observation group was divided into two subgroups:38 cases in DM group and 38 cases in DM+DF group)and 76 healthy people in the same period as the healthy control group.The level of cellular immunity in serum of the observation group and the healthy control group were compared,and the relationship between cellular immunity and diabetic foot was further explored Department.Results:there were significant differences in glycosylated hemoglobin and fasting blood glucose between the two groups(P<0.001);there were significant differences in CD8,CD25 and CD28 between the two groups(P<0.001).There were statistical differences in the course and age of diabetes between the two subgroups in the observation group(P<0.05);there were statistical differences in CD2,CD8,CD25 and CD28 between the two subgroups(P<0.05).Logistic regression analysis showed that CD8[or95%CI 0.920(0.850,0.995)]and CD25[or95%CI 0.764(0.619,0.943)]were independent influencing factors and risk factors of diabetic foot.Conclusion:the increased levels of CD8 and CD25 may increase the risk of diabetic foot.
基金National Natural Science Foundation of China(No.81774310)National Natural Science Foundation of China Youth Project(No.81804095)Shanghai Traditional Chinese Medicine Health Service Collaborative Innovation Center Project(No.ZYJKFW201701002).
文摘Objective: To observe the effect of Zizhu ointment on acute wound healing and its effect on the expression of autophagy proteins LC3 and Becin1. Method: SD rat model with acute skin injury were randomly divided into groups. The experimental group was treated with external application of Zizhu ointment, and the control group was given external saline. The wound healing rate of the two groups was compared at 4d, 7d, 14d and 21d after trauma. The morphology of wounds was observed by HE staining, and the autophagosomes of wounds were observed by transmission electron microscopy. Expression and changes of autophagy marker proteins Beclin-1 and LC3 were detected by immunohistochemistry and WB assay. Result: Compared with the control group, experimental group had a high healing rate, a short healing time, and a faster epithelialization rate. Under the electron microscope, autophagosomes of experimental group were reduced, and the expression of LC3 and Beclin-1 was significantly decreased. Conclusion: Zizhu ointment may promote wound healing by inhibiting autophagy activity of cells.
基金Youth programs of national natural science foundation of China(No.81804095)Funding project of Shanghai innovation center of TCM health service(No.ZYJKFW201701002
文摘Primary erythema limb pain is a very rare disease,and its pathogenesis is not clear.Usually,the pain symptoms of patients exist for a long time,which seriously affect the daily life of patients and increase the economic burden of patients.Professor Liu Guobin,who has been engaged in the diagnosis and treatment of peripheral vascular diseases for nearly 30 years,believes that heat-toxin and blood stasis collaterals are closely related to the disease,and emphasizes the principle of heat-toxin and evil qi causing the disease.It is emphasized that clearing heat and detoxification,promoting blood circulation and dispersing blood stasis are the main treatment principles.This paper selects a typical case to describe Professor Liu's clinical experience in the treatment of primary erythema limb pain by oral and external use of traditional Chinese medicine,so as to provide an effective method of traditional Chinese medicine for the treatment of primary erythema limb pain.
基金General project of national natural science foundation(No.81774310)The national natural science foundation youth project(No.81804095).
文摘Venous ulcer of lower extremities is a serious manifestation of venous insufficiency of lower extremities in the later stage,which is characterized by inflammatory exudation at the initial stage,followed by skin rupture,and finally forms the whole layer of skin atrophy and necrosis,which does not heal for a long time.How to promote ulcer healing is the most important problem in the treatment of venous ulcers of lower extremities.Vacuum sealing drainage(VSD)is constantly used in clinic and plays a unique advantage in wound healing.This paper mainly reviews the present situation of venous ulcer of lower extremities and summary,principle,application progress,attentions and prospect of VSD at home and abroad.
基金National Natural Science Foundation of China(81774310)National Natural Science Foundation of China Youth Project(81804095)Shanghai Synergistic Innovation Center for Chinese Medicine Health Services(ZYJKFW201701002).
文摘Objective:To explore the relationship between the changes of serum vitamin level in patients with type 2 diabetes mellitus and diabetic foot.Method Totally 68 patients with type 2 diabetes admitted to our hospital from September 2018 to March 2019 were selected as observation group(DM group:Totally 32 patients without diabetic foot group,DM+DF group of 34 patients)and 68 patients with physical examination in our hospital(healthy control group)at the same time,and the serum vitamin levels of the two groups of patients were compared.On this basis,the relationship between serum vitamin level and diabetic foot was further explored.Results There were differences between the observation group and the healthy control group in the history of hypertension,2h postprandial blood glucose and glycosylated hemoglobin between the two groups(all P<0.001).Compared with the vitamin levels of the observation group and the healthy control group,there were statistical differences in vitamin B6 and vitamin B1 and vitamin B9 and Vitamin C and vitamin D(P<0.001),but there were no differences in vitamin A,vitamin B2 and vitamin E(P>0.05).Compared with the vitamin levels in the two subgroups of the observation group,there were statistical differences in vitamin B6 and vitamin B1 and vitamin B9 and Vitamin C(P<0.001).Logistic regression analysis showed that vitamin B6[OR95%CI 0.633(0.434,0.924)],vitamin B1[OR95%CI 0.705(0.524,0.949),vitamin B9[OR95%CI 0.248(0.063,0.978),and Vitamin C[OR95%CI 0.584(0.409,0.835)were risk factors for diabetic foot.Conclusion Vitamin B6,vitamin B1,vitamin B9 and Vitamin C can be used as indicators to predict the risk of DF in T2DM patients.
基金National natural science foundation of China youth program(81804095)Shanghai TCM health service collaborative innovation center project(ZYJKFW201701002).
文摘Objective: To analyze the advantages of Traditional Chinese Cutting Therapy at the present stage and the characteristics of clinical research and application, so as to provide a basis for clinical treatment and research. Methods: Search the Chinese and English databases of relevant literatures on Traditional Chinese Cutting Therapy, and make statistics and Analysis on the distribution, types, distribution, indications and dominant diseases, the number of cases, clinical efficiency, intervention measures and operation sites of the published literatures. Results: A total of 311 articles were included. There are 40 indications for Traditional Chinese Cutting Therapy at this stage. The dominant diseases are psoriasis, asthma, facial paralysis, rhinitis, neurodermatitis, facial neuritis, chloasma, acne, migraine and insomnia. Traditional Chinese Cutting Therapy is closely related to meridian theory. At present, there are few theoretical and basic studies, the quality of relevant clinical studies is low, and the writing standard of research reports is inadequate. Conclusion: At present, the indications of Traditional Chinese Cutting Therapy are extensive, and the dominant diseases are concentrated, with dermatological diseases and neurological diseases as the main diseases. In the future, we should increase theoretical research and basic research, attach importance to the combination of meridian theory and Traditional Chinese Cutting Therapy, and standardize the implementation of clinical research and the writing of reports.
基金Youth Project of National Natural Science Foundation (81804095)
文摘Objective: To overview the systematic reviews of acupuncture for Post-stroke Dysphagia. Methods: The Chinese and English databases were searched for the systematic reviews of dysphagia after acupuncture treatment. The retrieval time was until October 30, 2019. The final literature was evaluated for bias risk, methodology quality and evidence quality by using ROBIS tool, AMSTER-2 scale and GRADE method. Results: 9 systematic reviews and 36 outcomes were included. ROBIS bias risk assessment results show that all the studies are high bias risk;AMSTER-2 methodology quality assessment results show that all the systems are of very low quality;GRADE grading shows that there are only 7 intermediate evidences in 36 clinical evidences, the rest are low-level evidences or very low-level evidences, and there are multiple intermediate evidences in the clinical efficacy of acupuncture in the treatment of dysphagia after stroke. In addition, acupuncture can significantly improve the scores of all kinds of swallowing function related scales, such as Tengdao food intake swallowing function grade, swallowing disorder specific quality of life score, standard swallowing function evaluation scale, and the incidence of adverse reactions is low. Conclusion: Acupuncture is effective in the treatment of dysphagia after stroke. It can improve the scores of various swallowing function scales and has high safety. However, the risk of systematic evaluation bias is high, the quality of methodology is low, and the level of clinical evidence is low as a whole. In the future, the relevant research design should be more rigorous, and the research report should be written in strict accordance with the PRISMA statement.
基金1.General Project of National Natural Science Foundation of China in 20172.Shanghai TCM Health Service Collaborative Innovation Center Project.Project No:1.817743102.ZYJKFW201701002.
文摘Objective: It is expected to establish a diabetic ulcer rat model infected with Escherichia coli to provide an appropriate model for the experimental study of diabetic foot ulcer. Method: 32 SD male rats were randomly divided into three groups including the common ulcer group (N), the diabetic ulcer group (DU) and the model group (M) and Befuji group (B). The stability and practicability of the model were evaluated by HE performed on the local skin tissue of the wounds and ELISA detection detecting the content of High sensitive c-reactive protein (HCRP), basic fibroblast growth factor (bFGF) in rat serum after modeling and HCRP, bFGF, tumor necrosis factor-alpha (TNF-α), advanced glycation end products (AGEs), Interleukin-1β (IL-1β) and Interleukin-6 (IL-6) levels after wound healing. Results: After modeling, the HCRP content in N group was lower than that in the other three groups. There was no significant difference in bFGF content between the groups. After wound healing, the HCRP content in serum of rats in each group showed statistical difference. The level of HCRP in group B was lower than that in group M. There was no difference in bFGF content between the groups. The level of bFGF in group B was higher than that in group N. The contents of AGEs in each group were statistically different. The contents of AGEs in group B were lower than those in group DU and group M. The contents of TNF-α, IL-1βand IL-6 in each group were different. The levels of TNF-α, IL-1βand IL-6 in group B were lower than those in group M. Compared with DU group, the levels of TNF-α, IL-1βin M group were higher . Wound healing time in group M was significantly delayed than that in group N, group DU and group B. Conclusion:This model is more in line with the local environmental characteristics of infected diabetic ulcer and provides a reliable tool for the study of diabetic infections.
基金NSFC(No.81774310)National natural science foundation of China youth program(No.81804095)Shanghai TCM health service collaborative innovation center project(No.ZYJKFW201701002).
文摘Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats were randomly divided into five groups: general ulcer group, diabetic ulcer group, diabetic ulcer treatment group, model group, and model treatment group. After modeling, take local skin tissue for HE staining.Detection of high-sensitivity C-reactive protein, fibroblast growth factor, tumor necrosis factor-α, advanced glycosylation products, interleukin-1β, interleukin-6, and nitric oxide in rat serum by enzyme-linked immunosorbent Level, and record the wound healing time, and evaluate the stability and practicability of the model. Results:The healing time of ulcer in the model group was significantly delayed compared with other groups (p <0.001). The serum H-CRP levels in the model group and the model treatment group were higher than those in the common ulcer group, the diabetic ulcer group, and the diabetic ulcer treatment group (p <0.05). After healing, the levels of H-CRP, bFGF, AGEs, TNF-α, IL-1β, IL-6, and NO in the serum of the diabetic ulcer group were higher than those in the common ulcer group (all p <0.05);The levels of TNF-α, IL-1β, IL-6, and NO were higher than those in other groups (all p <0.05);HCRP, AGEs, TNF-α, IL-1β, IL-6, and NO levels in the serum of the model treatment group They were all higher than those in diabetic ulcer treatment group (all p <0.05). Conclusion: This model is in line with the local environment of infectious diabetic ulcers and provides a reliable tool for the study of diabetic infections.
基金National natural science foundation(81774310)National natural science foundation of China youth program(81804095)Shanghai TCM health service collaborative innovation center project(ZYJKFW201701002).
文摘Venous hypertension is a potential mechanism for the formation of venous leg ulcers.Improving venous hypertension has become a key point in the treatment of venous leg ulcers.compression therapy is non-invasive and safe and has become the most basic method for the treatment of venous leg ulcers and runs through the whole treatment process.This article summarizes the preparation before pressure treatment,matters needing attention in use and different types of pressure devices,so as to provide safe and effective treatment schemes for the vast number of doctors.
基金National Natural Science Foundation ItemProject of Scientific Research Plan of Shanghai Municipal Science and Technology Commission.Project No:8177431016401902200.
文摘Diabetic foot is one of the serious complications of diabetes, which formed foot ulcer in the later stage of diabetes mellitus. The treatment of diabetic foot ulcer is difficult, greatly increasing the risk of amputation, which will seriously affect the quality of life of patients. Promoting the healing of ulcer in the early stage has become the guiding ideology for the treatment of diabetic foot ulcer. Macrophages, as important immune cells, play a regulatory role throughout the healing process of diabetic foot ulcers. Therefore, we can provide a new direction for the treatment of diabetic foot ulcers by deeply understanding the mechanism of macrophages and the healing of diabetic foot ulcers.
