Background Omeprazole, usually used in the antiplatelet therapy during percutaneous coronary intervention （PCI） in acute coronary syndrome （ACS）, has been reported to increase ischemic events in retrospective stud...Background Omeprazole, usually used in the antiplatelet therapy during percutaneous coronary intervention （PCI） in acute coronary syndrome （ACS）, has been reported to increase ischemic events in retrospective studies. However, other clinical trials gave paradoxical results. The aim of this study was to assess the effects of omeprazole on clopidogrel efficacy and clinical events.Methods All patients （n=172） received aspirin （loading dose 300 mg and maintenance dose 100 mg/d） and clopidogrel （loading dose 600 mg and maintenance dose 75 mg/d） during the therapy. They were randomized to receive omeprazole （20 mg/d） or placebo for 30 days. Residual platelet activities in the adenosine 5＇-diphosphate （ADP） pathway were detected on the fifth day after PCI with thrombelastography （TEG）-mapping. The clinical events were recorded after one month.Results According to the five levels of platelet activities, the frequency distributions of the inhibition rates were significantly different （P=0.0062）. However, no significant change was seen in the distribution among the highest or the lowest inhibiting levels （〉95% and 〈30% inhibition rate）. And there were no significant differences （P 〉0.05） in events incidence, while gastra-intesternal bleeding decreased in co-administration of omeprazole.Conclusions Omeprazole significantly blunts clopidogreal efficacy while not exacerbates ischimic events in ACS undergoing PCI. Omeprazaole even can decrease gastra-intestinal bleeding in those patients.展开更多
1.Introduction Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac causes.It is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symp...1.Introduction Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac causes.It is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symptoms that are atypical and not easily detected[1].According to a recent study,nearly 544000 patients suffer from SCD every year in China[2].展开更多
To the Editor:Otitis media with effusion(OME),also known as secretory otitis,is a disease in which secreted fluid accumulates in the middle ear cavity and is a major cause of hearing loss,both in children and adults.[...To the Editor:Otitis media with effusion(OME),also known as secretory otitis,is a disease in which secreted fluid accumulates in the middle ear cavity and is a major cause of hearing loss,both in children and adults.[1]Causes of OME include recurrent infections,inflammatory conditions,malformations,and eustachian tube dysfunctions.[2]Although most patients could recover spontaneously,by medication,or by surgery,some patients show frequent recurrence of otitis media for more than 3 months,called chronic otitis media with effusion(COME).[1]The pathogenesis of COME is not completely understood.Increasing evidence suggests a central role of immunologic cells and th&r associated cytokines in the chronic inflammation that characterizes COME.展开更多
p21^wafl/cipl, best known as a broad-specificity inhibitor of cyclin/cyclin-dependent kinase complexes, can interact with various target proteins, and this ability relies on its structural plasticity. Therefore, studi...p21^wafl/cipl, best known as a broad-specificity inhibitor of cyclin/cyclin-dependent kinase complexes, can interact with various target proteins, and this ability relies on its structural plasticity. Therefore, studies on the structural properties of p21 are very important to understand its structure-function relationship. However, detailed studies on its secondary structure and biophysical propertics have been comparatively sparse. A human p21 gene was cloned into the temperature expression vector pBV220 and transformed into Escherichia coli strain JM109. Recombinant protein was expressed as a non-fusion protein and purified by gel filtration and anion exchange chromatography. The purified protein was verified by Western blot and the functional activity was recognized by pull-down assay. Furthermore, circular dichroism, fluorescence spectroscopy, and fluorescence quenching methods were used to characterize the conformational properties of the purified protein. The results indicate that it was largely unstructured under the native solution conditions, and its tryptophan residues were exposed and located in a positively charged microenvironment. This study lays a good foundation for further study of p21 binding to its different partners.展开更多
The complex [(ButC5H’4)2YbCl]2, crystallized in the triclinic system,space group P1, with a=8. 392(3), b=9. 402(3), c=12. 418(5) , a=68. 78(3),P= 77. 43(3), γ=86. 41 (3)°, V= 891. 4(5) , Z=1, Dc= 1. 68 g/cm3, M...The complex [(ButC5H’4)2YbCl]2, crystallized in the triclinic system,space group P1, with a=8. 392(3), b=9. 402(3), c=12. 418(5) , a=68. 78(3),P= 77. 43(3), γ=86. 41 (3)°, V= 891. 4(5) , Z=1, Dc= 1. 68 g/cm3, Mr=901. 79, λ=0. 71069, F(000) = 464, μ= 56. 49 cm-1. T= 298 K. Final R=0.073, Rw= 0.081 for 2214 reflections with I> 3σ(I0). The molecule has a dimerstructure with two asymmetrical chlorine bridges. Two Bu5C5H4 and two chlorineatoms around the central atom Yb form a distorted tetrahedral geometry.展开更多
文摘Background Omeprazole, usually used in the antiplatelet therapy during percutaneous coronary intervention （PCI） in acute coronary syndrome （ACS）, has been reported to increase ischemic events in retrospective studies. However, other clinical trials gave paradoxical results. The aim of this study was to assess the effects of omeprazole on clopidogrel efficacy and clinical events.Methods All patients （n=172） received aspirin （loading dose 300 mg and maintenance dose 100 mg/d） and clopidogrel （loading dose 600 mg and maintenance dose 75 mg/d） during the therapy. They were randomized to receive omeprazole （20 mg/d） or placebo for 30 days. Residual platelet activities in the adenosine 5＇-diphosphate （ADP） pathway were detected on the fifth day after PCI with thrombelastography （TEG）-mapping. The clinical events were recorded after one month.Results According to the five levels of platelet activities, the frequency distributions of the inhibition rates were significantly different （P=0.0062）. However, no significant change was seen in the distribution among the highest or the lowest inhibiting levels （〉95% and 〈30% inhibition rate）. And there were no significant differences （P 〉0.05） in events incidence, while gastra-intesternal bleeding decreased in co-administration of omeprazole.Conclusions Omeprazole significantly blunts clopidogreal efficacy while not exacerbates ischimic events in ACS undergoing PCI. Omeprazaole even can decrease gastra-intestinal bleeding in those patients.
基金Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(No.2019-I2M-5-023)Hainan Provincial Science and Technology Major Project(No.ZDKJ201804)+2 种基金National Natural Science Foundation of China(No.81871611)National Natural Science Foundation of China(No.81760352)Project of Hainan Provincial Department of Education(No.Hnjg2019ZD-16)
文摘1.Introduction Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac causes.It is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symptoms that are atypical and not easily detected[1].According to a recent study,nearly 544000 patients suffer from SCD every year in China[2].
基金a grant from the National Natural Science Foundation of China(No.81770989).
文摘To the Editor:Otitis media with effusion(OME),also known as secretory otitis,is a disease in which secreted fluid accumulates in the middle ear cavity and is a major cause of hearing loss,both in children and adults.[1]Causes of OME include recurrent infections,inflammatory conditions,malformations,and eustachian tube dysfunctions.[2]Although most patients could recover spontaneously,by medication,or by surgery,some patients show frequent recurrence of otitis media for more than 3 months,called chronic otitis media with effusion(COME).[1]The pathogenesis of COME is not completely understood.Increasing evidence suggests a central role of immunologic cells and th&r associated cytokines in the chronic inflammation that characterizes COME.
基金Supported by the National Natural Science Foundation of China(Nos20225102, 20331020, 20325101 and 20473084)Hundred People Program of Chinese Academy of Sciences
文摘p21^wafl/cipl, best known as a broad-specificity inhibitor of cyclin/cyclin-dependent kinase complexes, can interact with various target proteins, and this ability relies on its structural plasticity. Therefore, studies on the structural properties of p21 are very important to understand its structure-function relationship. However, detailed studies on its secondary structure and biophysical propertics have been comparatively sparse. A human p21 gene was cloned into the temperature expression vector pBV220 and transformed into Escherichia coli strain JM109. Recombinant protein was expressed as a non-fusion protein and purified by gel filtration and anion exchange chromatography. The purified protein was verified by Western blot and the functional activity was recognized by pull-down assay. Furthermore, circular dichroism, fluorescence spectroscopy, and fluorescence quenching methods were used to characterize the conformational properties of the purified protein. The results indicate that it was largely unstructured under the native solution conditions, and its tryptophan residues were exposed and located in a positively charged microenvironment. This study lays a good foundation for further study of p21 binding to its different partners.
文摘The complex [(ButC5H’4)2YbCl]2, crystallized in the triclinic system,space group P1, with a=8. 392(3), b=9. 402(3), c=12. 418(5) , a=68. 78(3),P= 77. 43(3), γ=86. 41 (3)°, V= 891. 4(5) , Z=1, Dc= 1. 68 g/cm3, Mr=901. 79, λ=0. 71069, F(000) = 464, μ= 56. 49 cm-1. T= 298 K. Final R=0.073, Rw= 0.081 for 2214 reflections with I> 3σ(I0). The molecule has a dimerstructure with two asymmetrical chlorine bridges. Two Bu5C5H4 and two chlorineatoms around the central atom Yb form a distorted tetrahedral geometry.
