BACKGROUND Cholecystectomy is a successful treatment option for gallstones,although the inci-dence of colorectal cancer(CRC)has notably increased in post-cholecystectomy(PC)patients.However,it remains uncertain whethe...BACKGROUND Cholecystectomy is a successful treatment option for gallstones,although the inci-dence of colorectal cancer(CRC)has notably increased in post-cholecystectomy(PC)patients.However,it remains uncertain whether the altered mucosal micro-biota in the ascending colon is related.In total,30 PC patients and 28 healthy controls underwent colonoscopies to collect mucosal biopsy samples.PC patients were divided based on their clinical features.Then,16S-rRNA gene sequencing was used to analyze the amplicon,alpha diversity,beta diversity,and composition of the bacterial communities.Addi-tionally,the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt)database,sourced from the Kyoto Encyclopedia of Genes and Genomes,was used to predict the functional capabilities of the bac-teria.RESULTS PC patients were comparable with healthy controls.However,PC patients older than 60 years had a distinct composition compared to those under 60 years old.Bacteroidetes richness was considerably higher at the phylum level in PC patients.Bacteroides,Parabacteroides,and Bilophila were more abundant in the PC group than in the control group.Furthermore,PC patients exhibited greater enrichment in metabolic pathways,specifically those related to lipopolysaccharide biosynthesis and vancomycin group antibiotic production,than controls.CONCLUSION This study indicated that the mucosal microbiota in PC patients was altered,perhaps offering new perspectives on the treatment possibilities for CRC and diarrhea following cholecystectomy.展开更多
RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed ...RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.展开更多
BACKGROUND Nodular lymphoid hyperplasia(NLH)in the small intestine is a rare benign lesion characterized by multiple small nodules on the intestinal surface.Patients with terminal ileal NLH may experience long-term ab...BACKGROUND Nodular lymphoid hyperplasia(NLH)in the small intestine is a rare benign lesion characterized by multiple small nodules on the intestinal surface.Patients with terminal ileal NLH may experience long-term abdominal pain,diarrhea,and abdominal distension,among other symptoms.Supplementation with probiotics could mitigate these symptoms.NLH is linked to the immune system,and it may result from accumulation of plasma-cell precursors due to a maturational defect during the development of B lymphocytes.The intestinal microbiome plays an essential role in the immune system.Thus,we speculate that the gut flora plays a key role in terminal ileal NLH.AIM To explore the correlation between intestinal flora and terminal ileal NLH.METHODS We collected mucosal biopsy samples that were obtained via colonoscopy from 15 patients with terminal ileal NLH(the test group)and 15 normal subjects(the control group).We subsequently performed 16 S-r RNA gene amplicon sequencing of these samples,and the results were evaluated using alpha diversity,beta diversity and microbial composition analyses.The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States was used to predict the metabolic pathways and orthologous groups according to the Kyoto Encyclopedia of Genes and Genomes database.RESULTS Compared with the control group,the terminal ileal NLH group showed an increased alpha diversity(P<0.05).The overall intestinal microbiota in the NLH group was significantly different from that of the control group(P<0.05),implying that there was the dysbiosis in the terminal ileal NLH patients.The relative abundance of phylum Bacteroidetes was significantly lower in the NLH group,while that of Patescibacteria and Campilobacterota was significantly higher.The genus Bacteroides was the dominant gut microbiota in both groups,but its abundance was significantly lower in the test group than it was in the control group.Conversely,the relative abundances of Haemophilus,Streptococcus,Pseudomonas,Actinomyces,TM7 X,Fusobacte展开更多
Aim: The purpose of this study is to investigate the incidence, distribution and risk factors of cerebral microbleeds (CMBs) and the relation between CMBs and inflammation in ischemic cerebrovascular disease. Methods:...Aim: The purpose of this study is to investigate the incidence, distribution and risk factors of cerebral microbleeds (CMBs) and the relation between CMBs and inflammation in ischemic cerebrovascular disease. Methods: Two hundred and one patients without acute infarction or transient ischemic attack were enrolled. The presence and number of CMB were assessed on susceptibility-weighted imaging. The traditional risk factors of CMB were recorded. Levels of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and matrix metalloproteinase-9 (MMP-9) were tested. Logistic regression analyses were used for multiple-factor analysis of risk factors of CMB. Results: Of the 201 patients, 49 (24.38%) had CMB. Multivariate logistic regression analyses showed that the age, the prevalence of hypertension, silent lacunar infarction, white matter lesion, Montreal Cognitive Assessment Score, the using rate of antithrombotic drugs and levels of hs-CRP, IL-6, MMP-9 were the risk factors for CMB. After adjustments for traditional risk factors, inflammatory marker levels remained to be associated with CMBs. The adjusted odd ratios of hs-CRP, IL-6 and MMP-9 were 1.745 (1.342-2.270), 1.223 (1.018-1.533) and 1.284 (1.082-1.423), respectively. Furthermore, inflammatory marker levels were the risk factor for deep or infratentorial CMBs and lobar CMBs. Conclusion: The age, prevalence of hypertension, silent lacunar infarction, white matter lesion, MoCA Score, the using rate of antithrombotic drugs and serum hs-CRP, IL-6, and MMP-9 levels were the independent risk factors for CMBs.展开更多
基金the Shanghai Natural Science Foundation Project,No.22ZR1453500and Jiading District Health Commission Scientific Project,No.2023-KY-01.
文摘BACKGROUND Cholecystectomy is a successful treatment option for gallstones,although the inci-dence of colorectal cancer(CRC)has notably increased in post-cholecystectomy(PC)patients.However,it remains uncertain whether the altered mucosal micro-biota in the ascending colon is related.In total,30 PC patients and 28 healthy controls underwent colonoscopies to collect mucosal biopsy samples.PC patients were divided based on their clinical features.Then,16S-rRNA gene sequencing was used to analyze the amplicon,alpha diversity,beta diversity,and composition of the bacterial communities.Addi-tionally,the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt)database,sourced from the Kyoto Encyclopedia of Genes and Genomes,was used to predict the functional capabilities of the bac-teria.RESULTS PC patients were comparable with healthy controls.However,PC patients older than 60 years had a distinct composition compared to those under 60 years old.Bacteroidetes richness was considerably higher at the phylum level in PC patients.Bacteroides,Parabacteroides,and Bilophila were more abundant in the PC group than in the control group.Furthermore,PC patients exhibited greater enrichment in metabolic pathways,specifically those related to lipopolysaccharide biosynthesis and vancomycin group antibiotic production,than controls.CONCLUSION This study indicated that the mucosal microbiota in PC patients was altered,perhaps offering new perspectives on the treatment possibilities for CRC and diarrhea following cholecystectomy.
基金The Nation "863" Program of China(2006AA02A226)The Joint Funds of National Science Foundation of China (U0632010)+2 种基金The State KeyLaboratory of Phytochemistry and Plant Resources in West ChinaChinese Academy of Sciences (O807B11211, O807E21211)"211 grant of MOE"
文摘RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
基金Supported by the Science and Technology Project of Jiading Hospital,Shanghai General Hospital,No.202134A,No.202125A。
文摘BACKGROUND Nodular lymphoid hyperplasia(NLH)in the small intestine is a rare benign lesion characterized by multiple small nodules on the intestinal surface.Patients with terminal ileal NLH may experience long-term abdominal pain,diarrhea,and abdominal distension,among other symptoms.Supplementation with probiotics could mitigate these symptoms.NLH is linked to the immune system,and it may result from accumulation of plasma-cell precursors due to a maturational defect during the development of B lymphocytes.The intestinal microbiome plays an essential role in the immune system.Thus,we speculate that the gut flora plays a key role in terminal ileal NLH.AIM To explore the correlation between intestinal flora and terminal ileal NLH.METHODS We collected mucosal biopsy samples that were obtained via colonoscopy from 15 patients with terminal ileal NLH(the test group)and 15 normal subjects(the control group).We subsequently performed 16 S-r RNA gene amplicon sequencing of these samples,and the results were evaluated using alpha diversity,beta diversity and microbial composition analyses.The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States was used to predict the metabolic pathways and orthologous groups according to the Kyoto Encyclopedia of Genes and Genomes database.RESULTS Compared with the control group,the terminal ileal NLH group showed an increased alpha diversity(P<0.05).The overall intestinal microbiota in the NLH group was significantly different from that of the control group(P<0.05),implying that there was the dysbiosis in the terminal ileal NLH patients.The relative abundance of phylum Bacteroidetes was significantly lower in the NLH group,while that of Patescibacteria and Campilobacterota was significantly higher.The genus Bacteroides was the dominant gut microbiota in both groups,but its abundance was significantly lower in the test group than it was in the control group.Conversely,the relative abundances of Haemophilus,Streptococcus,Pseudomonas,Actinomyces,TM7 X,Fusobacte
文摘Aim: The purpose of this study is to investigate the incidence, distribution and risk factors of cerebral microbleeds (CMBs) and the relation between CMBs and inflammation in ischemic cerebrovascular disease. Methods: Two hundred and one patients without acute infarction or transient ischemic attack were enrolled. The presence and number of CMB were assessed on susceptibility-weighted imaging. The traditional risk factors of CMB were recorded. Levels of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and matrix metalloproteinase-9 (MMP-9) were tested. Logistic regression analyses were used for multiple-factor analysis of risk factors of CMB. Results: Of the 201 patients, 49 (24.38%) had CMB. Multivariate logistic regression analyses showed that the age, the prevalence of hypertension, silent lacunar infarction, white matter lesion, Montreal Cognitive Assessment Score, the using rate of antithrombotic drugs and levels of hs-CRP, IL-6, MMP-9 were the risk factors for CMB. After adjustments for traditional risk factors, inflammatory marker levels remained to be associated with CMBs. The adjusted odd ratios of hs-CRP, IL-6 and MMP-9 were 1.745 (1.342-2.270), 1.223 (1.018-1.533) and 1.284 (1.082-1.423), respectively. Furthermore, inflammatory marker levels were the risk factor for deep or infratentorial CMBs and lobar CMBs. Conclusion: The age, prevalence of hypertension, silent lacunar infarction, white matter lesion, MoCA Score, the using rate of antithrombotic drugs and serum hs-CRP, IL-6, and MMP-9 levels were the independent risk factors for CMBs.
文摘通过分析中医药治疗微血管性心绞痛(microvascular angina,MVA)的随机对照试验(RCT)中结局指标使用现状,探讨存在的问题和可能的解决策略,为开展高质量临床试验设计和建立MVA核心结局指标集提供参考。该研究系统检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、中国生物医学文献服务系统(SinoMed)、PubMed、EMbase、Cochrane Library、Web of Science和2个临床试验注册中心,根据预先制定的标准筛选中医药治疗MVA的RCT并提取资料,采用Cochrane工作组的偏倚风险评估工具对纳入文献进行方法学质量评价,汇总分析结局指标使用情况。最终纳入69篇RCTs,提取到100种结局指标,出现频次430次,分为8个指标域:临床有效率、症状体征、理化检查、中医疗效、安全性、生活质量、经济学评估、远期预后,其中理化检查的种类及频次最多(70种,211次),其次为临床有效率(7种,73次)、症状体征(7种,54次)等。使用频次较高的结局指标依次为不良反应、心绞痛发作频率、临床疗效、内皮素-1、运动平板持续总时间、超敏C反应蛋白等。目前中医药治疗MVA的RCT尚存在以下问题:不良反应报告不规范、指标种类多但使用频次低、不重视终点指标的应用、中医辨证及证候疗效指标使用不足、评价标准不规范且未体现中医基本特点等。建议制定统一规范的MVA证候诊断标准,在此基础上建立以患者报告结局为出发点突出中医特色的MVA疗效评价体系与核心结局指标集,提高临床研究水平和研究价值。
文摘结构面的几何参数和力学参数随机分布导致岩体工程的稳定性具有高度不确定性.以我国某地下水封石油储备库项目为背景,把结构面倾向、倾角、黏聚力及内摩擦角作为随机变量,运用可靠度理论,在数值模拟的基础上,研究了围岩块体稳定性和支护系统可靠性,并对支护参数进行检验与优化.结果表明:Ⅰ~Ⅳ级围岩块体稳定性可靠度指标分别为4.23,1.68,0.65和-0.07;对Ⅱ级、Ⅲ级和Ⅳ级围岩块体采取支护措施后,可靠度指标分别为3.3,3.0和2.0;并对其支护间排距进行优化,推荐值分别为2.8,2.0和1.6 m.
