Rheumatoid arthritis(RA)is a common autoimmune disease leading to pain,disability,and even death.Although studies have revealed that aberrant activation of STING was implicated in various autoimmune diseases,the role ...Rheumatoid arthritis(RA)is a common autoimmune disease leading to pain,disability,and even death.Although studies have revealed that aberrant activation of STING was implicated in various autoimmune diseases,the role of STING in RA remains unclear.In the current study,we demonstrated that STING activation was pivotal in RA pathogenesis.As the accumulation of dsDNA,a specific stimulus for STING,is a feature of RA,we developed a spherical polyethyleneimine-coated mesoporous polydopamine nanoparticles loaded with STING antagonist C-176(PEI-PDA@C-176 NPs)for treating RA.The fabricated NPs with biocompatibility had high DNA adsorption ability and could effectively inhibit the STING pathway and inflammation in macrophages.Intra-articular administration of PEI-PDA@C-176 NPs could effectively reduce joint damage in mice models of dsDNA-induced arthritis and collagen-induced arthritis by inhibiting STING pathway.We concluded that materials with synergistic effects of STING inhibition might be an efficacious strategy to treat RA.展开更多
Nanoliposomes have a broad range of applications in the treatment of autoimmune inflammatory diseases because of their ability to considerably enhance drug transport.For their clinical application,nanoliposomes must b...Nanoliposomes have a broad range of applications in the treatment of autoimmune inflammatory diseases because of their ability to considerably enhance drug transport.For their clinical application,nanoliposomes must be able to realize on-demand release of drugs at disease sites to maximize drug-delivery efficacy and minimize side effects.Therefore,responsive drug-release strategies for inflammation treatment have been explored;however,no specific design has been realized for a responsive drug-delivery system based on pyroptosis-related inflammation.Herein,we report a pioneering strategy for self-adaptive pyroptosis-responsive liposomes(R8-cardiolipin-containing nanoliposomes encapsulating dimethyl fumarate,RC-NL@DMF)that pre-cisely release encapsulated anti-pyroptotic drugs into pyroptotic cells.The activated key pyroptotic protein,the N-terminal domain of gasdermin E,selectively integrates with the cardiolipin of liposomes,thus forming pores for controlled drug release,pyroptosis,and inflammation inhibition.Therefore,RC-NL@DMF exhibited effective therapeutic efficacies to alleviate autoimmune inflammatory damages in zymosan-induced arthritis mice and dextran sulfate sodium-induced inflammatory bowel disease mice.Our novel approach holds great promise for self-adaptive pyroptosis-responsive on-demand drug delivery,suppressing pyroptosis and treating autoimmune inflammatory diseases.展开更多
An experiment on 100 k J laser facility is performed to study the motive features and radiation properties of plasmas from different areas inside gas-filled cylindrical hohlraums.These hohlraums are designed to posses...An experiment on 100 k J laser facility is performed to study the motive features and radiation properties of plasmas from different areas inside gas-filled cylindrical hohlraums.These hohlraums are designed to possess one open end and one laser entrance hole(LEH)with different diameters,which would or not result in the blocking of the LEH.An x-ray streak camera that is set at 16 degrees with respect to the hohlraum axis is applied to acquire the timeresolved x-ray images from the open end.Based on the images,we can study the evolutions of the wall plasma,corona bubble plasma and LEH plasma simultaneously through an equivalent view field of hohlraum interior.Multi-group flat response x-ray detectors are applied to measure the x-ray fluxes.In order to understand these characteristics,our two-dimensional radiation hydrodynamic code is used to simulate the experimental results.For the accuracy of reproduction,dielectronic recombination and two parameter corrections are applied in our code.Based on the comparison between experiments and simulations,we quantitatively understand the blocking process of LEH and the motion effects of other plasmas.The calibrated code is beneficial to design the gas-filled hohlraum in a nearby parameter space,especially the limit size of LEH.展开更多
Due to the fact that a high share of renewable energy sources(RESs)are connected to high-voltage direct current(HVDC)sending-end AC power systems,the voltage and frequency regulation capabilities of HVDC sending-end A...Due to the fact that a high share of renewable energy sources(RESs)are connected to high-voltage direct current(HVDC)sending-end AC power systems,the voltage and frequency regulation capabilities of HVDC sending-end AC power systems have diminished.This has resulted in potential system operating problems such as overvoltage and overfrequency,which occur simultaneously when block faults exist in the HVDC link.In this study,a steady-state voltage security-constrained optimal frequency control method for weak HVDC sending-end AC power systems is proposed.The integrated virtual inertia control of RESs is employed for system frequency regulation.Additional dynamic reactive power compensation devices are utilized to control the voltage of all nodes meet voltage security constraints.Then,an optimization model that simultaneously considers the frequency and steady-state voltage security constraints for weak HVDC sending-end AC power systems is established.The optimal control scheme with the minimum total cost of generation tripping and additional dynamic reactive power compensation required is obtained through the optimization solution.Simulations are conducted on a modified IEEE 9-bus test system and practical Qing-Yu line commutated converter based HVDC(LCC-HVDC)sending-end AC power system to verify the effectiveness of the proposed method.展开更多
Since Shuji Nakamura first demonstrated the nitride laser in 1996[1],the domain of semiconductor laser technology has undergone a period of remarkable growth[2,3].Al In Ga N-based diode lasers(LDs)have proven their ex...Since Shuji Nakamura first demonstrated the nitride laser in 1996[1],the domain of semiconductor laser technology has undergone a period of remarkable growth[2,3].Al In Ga N-based diode lasers(LDs)have proven their exceptional capabilities across a spectrum of pivotal applications,including high-density data storage,laser displays,laser lighting,and quantum technology[4].展开更多
The China Central Orogenic System(CCOS),extending in an east-west direction in the middle part of China,is composed of the Early Paleozoic Altyn-North Qilian-North Qaidam-East Kunlun-North Qinling-North Tongbai orogen...The China Central Orogenic System(CCOS),extending in an east-west direction in the middle part of China,is composed of the Early Paleozoic Altyn-North Qilian-North Qaidam-East Kunlun-North Qinling-North Tongbai orogens in the west and the Late Paleozoic to Early Mesozoic South Tongbai-Hong'an-Dabie-Sulu orogens in the east.They were produced by oceanic subduction and continental subduction/collision during the closure of the Proto-Tethys and the Paleo-Tethys oceans,respectively.Different types of metamorphic rocks with various ages are extensively exposed in these orogens,and they were produced at different geothermal gradients in different stages during the tectonic evolution of convergent continental margins,making them ideal targets to reconstruct the spatiotemporal evolution of the Eastern Tethys tectonic domain.In this article,an integrated study of metamorphic temperature(T)-pressure(P)-time(t)records is presented for metamorphic rocks along the CCOS,aiming to ascertain the change of metamorphic T/P ratios in both time and space,and then shed light on the tectonic evolution of the East Tethys tectonic domain in association with the thermal state change of convergent continental margins.The results indicate that despite the difference in metamorphic ages,metamorphic rocks in different orogens show a common trend with clockwise P-T-t paths.With respect to plate convergence for subduction and collision,regional metamorphism is categorized into three stages:(1)an early convergent stage,corresponding to low T/P Alpine-type blueschist-to eclogite-facies high-P to ultrahigh-P metamorphism;(2)a later convergent stage,corresponding to the medium T/P Barrovian-type medium-P amphibolite to high-P granulite-facies metamorphism;and(3)a post-convergent stage,corresponding to the high T/P Buchan-type lowP amphibolite to MP granulite-facies metamorphism.Nonetheless,a few metamorphic rocks only record a two-sage metamorphic evolution,with an early Barrovian-type high-P granulite-facies metamorphism and a late Buchan-type lo展开更多
Artificial metamaterials have attracted widespread attention of research communities due to their anomalous physical properties compared to those of conventional materials.In this study,we designed a three-dimensiona...Artificial metamaterials have attracted widespread attention of research communities due to their anomalous physical properties compared to those of conventional materials.In this study,we designed a three-dimensional(3D)lightweight metaarchitecture consisting of 6-connected anti-chiral honeycombs.The mechanical properties(e.g.Young’s modulus,compression strength,and Poisson’s ratio)of the proposed meta-architecture could be programmed by adjusting a series of geometric parameters,as shown through numerical simulations.Moreover,an optically sensitive polymer-based 3D meta-architecture with 6-connected anti-chiral features was constructed by the stereolithography method.Owing to the regulation of the negative Poisson’s ratio,3D meta-architecture achieved a greater ductility under compression than those of traditional truss structures while retaining a relatively high strength and low density.Compression experiments validated the excellent tunability of the mechanical properties of the proposed 3D 6-connected antichiral structure.The results suggest the promising applications of this structure in lightweight aircraft,vibration isolation,and mechanical sensors.展开更多
The first laser–plasma interaction experiment using lasers of eight beams grouped into one octad has been conducted on the Shenguang Octopus facility.Although each beam intensity is below its individual threshold for...The first laser–plasma interaction experiment using lasers of eight beams grouped into one octad has been conducted on the Shenguang Octopus facility.Although each beam intensity is below its individual threshold for stimulated Brillouin backscattering(SBS),collective behaviors are excited to enhance the octad SBS.In particular,when two-color/cone lasers with wavelength separation 0.3 nm are used,the backward SBS reflectivities show novel behavior in which beams of longer wavelength achieve higher SBS gain.This property of SBS can be attributed to the rotation of the wave vectors of common ion acoustic waves due to the competition of detunings between geometrical angle and wavelength separation.This mechanism is confirmed using massively parallel supercomputer simulations with the three-dimensional laser–plasma interaction code LAP3D.展开更多
In this research,the dynamics of wet spray nozzles with different geometries,used to accelerate shotcrete,are investigated on the basis of a suitable three-dimensional mathematical model and related numerical method.S...In this research,the dynamics of wet spray nozzles with different geometries,used to accelerate shotcrete,are investigated on the basis of a suitable three-dimensional mathematical model and related numerical method.Simulations have been conducted in the frame of the SIMPLEC algorithm.The k-εturbulence model has been used to account for turbulent effects.The study shows that when the angle of the convergent section is less than 3°,it has a scarce effect on the dynamics of the jet of shotcrete;with the increase of the convergence angle,the shotcrete jet velocity decreases and the nozzle wear increases;when this angle is greater than 6°,the concrete outlet jet velocity is very small and the nozzle can easily be blocked.Experimental results are in good agreement with the outcomes of the numerical simulations,which indicates that the used approach is reliable.展开更多
A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contamin...A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contaminants in DPW,which was pretreated with a membrane bioreactor(MBR).The strain was identified as Arthrospira platensis(A.platensis)based on its morphological characteristics and the 16S rDNA sequencing analysis.The effect of chrominance on growth of A.platensis ZJWST-S1 was investigated in a field raceway pond filled with MBR effluent or MBR effluent decolorized with ozonation.After ozonation,the chrominance of MBR effluent was decreased from 700 mg Pt/L to 150 mg Pt/L.Two runs of cultivation showed that A.platensis ZJWST-S1 grew faster in the ozone decolorized MBR effluent,the averaged biomass concentration being 0.907 g/L after 10 days of cultivation,close to that in a Zarrouk medium(0.969 g/L).By comparison,the biomass grew much slower in the non-decolorized MBR effluent(0.624 g/L).The pollutant removal was also benefited from the accelerative growth of A.platensis ZJWST-S1 in the decolorized MBR effluent.Almost all ammonium,61.2%of nitrate and 68.1%of phosphate were removed by the A.platensis ZJWST-S1 in the decolorized MBR effluent,much higher than the corresponding values of almost all ammonium,25.4%of nitrate and 36.5%of phosphate in the MBR effluent.Furthermore,the Arthrospira biomass harvested from the ozone decolorized MBR effluent after 10 d cultivation was with crude protein content of 59.1%±3.5%in dry algae powder.The content of Pb,As,Cd and Hg in biomass was also low enough to meet the Chinese Arthrospira Standard for Animal Feed(GB/T 17243-1998).This study showed that the new strain A.platensis ZJWST-S1 possessed potential to be used for producing animal feed and simultaneous removal of nitrogen and phosphorus in DPW.展开更多
基金supported by the National Natural Science Foundation of China(81772382,22161132027 and 82102599)the Key Research Program in Zhejiang Province,Science Technology Department of Zhejiang Province(2020C03042)the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SN-ZJU-SIAS-006).
文摘Rheumatoid arthritis(RA)is a common autoimmune disease leading to pain,disability,and even death.Although studies have revealed that aberrant activation of STING was implicated in various autoimmune diseases,the role of STING in RA remains unclear.In the current study,we demonstrated that STING activation was pivotal in RA pathogenesis.As the accumulation of dsDNA,a specific stimulus for STING,is a feature of RA,we developed a spherical polyethyleneimine-coated mesoporous polydopamine nanoparticles loaded with STING antagonist C-176(PEI-PDA@C-176 NPs)for treating RA.The fabricated NPs with biocompatibility had high DNA adsorption ability and could effectively inhibit the STING pathway and inflammation in macrophages.Intra-articular administration of PEI-PDA@C-176 NPs could effectively reduce joint damage in mice models of dsDNA-induced arthritis and collagen-induced arthritis by inhibiting STING pathway.We concluded that materials with synergistic effects of STING inhibition might be an efficacious strategy to treat RA.
基金sustained by the National Natural Science Foundation of China(82072512,52273152,22161132027)Zhejiang Provincial Natural Science Foundation of China(LY23H060013,LY21H070001,LY20H160044,LBY21H060003).
文摘Nanoliposomes have a broad range of applications in the treatment of autoimmune inflammatory diseases because of their ability to considerably enhance drug transport.For their clinical application,nanoliposomes must be able to realize on-demand release of drugs at disease sites to maximize drug-delivery efficacy and minimize side effects.Therefore,responsive drug-release strategies for inflammation treatment have been explored;however,no specific design has been realized for a responsive drug-delivery system based on pyroptosis-related inflammation.Herein,we report a pioneering strategy for self-adaptive pyroptosis-responsive liposomes(R8-cardiolipin-containing nanoliposomes encapsulating dimethyl fumarate,RC-NL@DMF)that pre-cisely release encapsulated anti-pyroptotic drugs into pyroptotic cells.The activated key pyroptotic protein,the N-terminal domain of gasdermin E,selectively integrates with the cardiolipin of liposomes,thus forming pores for controlled drug release,pyroptosis,and inflammation inhibition.Therefore,RC-NL@DMF exhibited effective therapeutic efficacies to alleviate autoimmune inflammatory damages in zymosan-induced arthritis mice and dextran sulfate sodium-induced inflammatory bowel disease mice.Our novel approach holds great promise for self-adaptive pyroptosis-responsive on-demand drug delivery,suppressing pyroptosis and treating autoimmune inflammatory diseases.
基金supported by National Natural Science Foundation of China(Nos.12075219,12105269 and 12175210)。
文摘An experiment on 100 k J laser facility is performed to study the motive features and radiation properties of plasmas from different areas inside gas-filled cylindrical hohlraums.These hohlraums are designed to possess one open end and one laser entrance hole(LEH)with different diameters,which would or not result in the blocking of the LEH.An x-ray streak camera that is set at 16 degrees with respect to the hohlraum axis is applied to acquire the timeresolved x-ray images from the open end.Based on the images,we can study the evolutions of the wall plasma,corona bubble plasma and LEH plasma simultaneously through an equivalent view field of hohlraum interior.Multi-group flat response x-ray detectors are applied to measure the x-ray fluxes.In order to understand these characteristics,our two-dimensional radiation hydrodynamic code is used to simulate the experimental results.For the accuracy of reproduction,dielectronic recombination and two parameter corrections are applied in our code.Based on the comparison between experiments and simulations,we quantitatively understand the blocking process of LEH and the motion effects of other plasmas.The calibrated code is beneficial to design the gas-filled hohlraum in a nearby parameter space,especially the limit size of LEH.
基金supported in part by the National Key R&D Program of China(No.2022YFB2402700)the Science and Technology Project of State Grid Corporation of China(No.52272222001J).
文摘Due to the fact that a high share of renewable energy sources(RESs)are connected to high-voltage direct current(HVDC)sending-end AC power systems,the voltage and frequency regulation capabilities of HVDC sending-end AC power systems have diminished.This has resulted in potential system operating problems such as overvoltage and overfrequency,which occur simultaneously when block faults exist in the HVDC link.In this study,a steady-state voltage security-constrained optimal frequency control method for weak HVDC sending-end AC power systems is proposed.The integrated virtual inertia control of RESs is employed for system frequency regulation.Additional dynamic reactive power compensation devices are utilized to control the voltage of all nodes meet voltage security constraints.Then,an optimization model that simultaneously considers the frequency and steady-state voltage security constraints for weak HVDC sending-end AC power systems is established.The optimal control scheme with the minimum total cost of generation tripping and additional dynamic reactive power compensation required is obtained through the optimization solution.Simulations are conducted on a modified IEEE 9-bus test system and practical Qing-Yu line commutated converter based HVDC(LCC-HVDC)sending-end AC power system to verify the effectiveness of the proposed method.
基金funded by the National Key Research and Development Program(Grant No.2023YFB4604400)the National Natural Science Foundation of China(Grant Nos.62225405,62350002,61991443)+2 种基金the Key R&D Program of Jiangsu ProvinceChina(Grant No.BE2020004)the Collaborative Innovation Centre of Solid-State Lighting and EnergySaving Electronics。
文摘Since Shuji Nakamura first demonstrated the nitride laser in 1996[1],the domain of semiconductor laser technology has undergone a period of remarkable growth[2,3].Al In Ga N-based diode lasers(LDs)have proven their exceptional capabilities across a spectrum of pivotal applications,including high-density data storage,laser displays,laser lighting,and quantum technology[4].
基金supported by the National Natural Science Foundation of China (Grant No.92155306)。
文摘The China Central Orogenic System(CCOS),extending in an east-west direction in the middle part of China,is composed of the Early Paleozoic Altyn-North Qilian-North Qaidam-East Kunlun-North Qinling-North Tongbai orogens in the west and the Late Paleozoic to Early Mesozoic South Tongbai-Hong'an-Dabie-Sulu orogens in the east.They were produced by oceanic subduction and continental subduction/collision during the closure of the Proto-Tethys and the Paleo-Tethys oceans,respectively.Different types of metamorphic rocks with various ages are extensively exposed in these orogens,and they were produced at different geothermal gradients in different stages during the tectonic evolution of convergent continental margins,making them ideal targets to reconstruct the spatiotemporal evolution of the Eastern Tethys tectonic domain.In this article,an integrated study of metamorphic temperature(T)-pressure(P)-time(t)records is presented for metamorphic rocks along the CCOS,aiming to ascertain the change of metamorphic T/P ratios in both time and space,and then shed light on the tectonic evolution of the East Tethys tectonic domain in association with the thermal state change of convergent continental margins.The results indicate that despite the difference in metamorphic ages,metamorphic rocks in different orogens show a common trend with clockwise P-T-t paths.With respect to plate convergence for subduction and collision,regional metamorphism is categorized into three stages:(1)an early convergent stage,corresponding to low T/P Alpine-type blueschist-to eclogite-facies high-P to ultrahigh-P metamorphism;(2)a later convergent stage,corresponding to the medium T/P Barrovian-type medium-P amphibolite to high-P granulite-facies metamorphism;and(3)a post-convergent stage,corresponding to the high T/P Buchan-type lowP amphibolite to MP granulite-facies metamorphism.Nonetheless,a few metamorphic rocks only record a two-sage metamorphic evolution,with an early Barrovian-type high-P granulite-facies metamorphism and a late Buchan-type lo
基金financially supported by the National Natural Science Foundation of China(Grant No.52073132),Science Fund for Distinguished Young Scholars of Gansu Province(Grant No.18JR3RA263),the Fundamental Research Funds for the Central Universities(Grant No.lzujbky-2020-ct05),the Talent Innovation and Entrepreneurship Project of Lanzhou(Grant No:2019-RC-42),and the 2019 Civil-Military Integration Project of Lanzhou(Grant No:GF-2019-ZA-QT-05)。
文摘Artificial metamaterials have attracted widespread attention of research communities due to their anomalous physical properties compared to those of conventional materials.In this study,we designed a three-dimensional(3D)lightweight metaarchitecture consisting of 6-connected anti-chiral honeycombs.The mechanical properties(e.g.Young’s modulus,compression strength,and Poisson’s ratio)of the proposed meta-architecture could be programmed by adjusting a series of geometric parameters,as shown through numerical simulations.Moreover,an optically sensitive polymer-based 3D meta-architecture with 6-connected anti-chiral features was constructed by the stereolithography method.Owing to the regulation of the negative Poisson’s ratio,3D meta-architecture achieved a greater ductility under compression than those of traditional truss structures while retaining a relatively high strength and low density.Compression experiments validated the excellent tunability of the mechanical properties of the proposed 3D 6-connected antichiral structure.The results suggest the promising applications of this structure in lightweight aircraft,vibration isolation,and mechanical sensors.
基金supported by the Natural Science Foundation of China(Grant Nos.11975059,12005021,and 11875241).
文摘The first laser–plasma interaction experiment using lasers of eight beams grouped into one octad has been conducted on the Shenguang Octopus facility.Although each beam intensity is below its individual threshold for stimulated Brillouin backscattering(SBS),collective behaviors are excited to enhance the octad SBS.In particular,when two-color/cone lasers with wavelength separation 0.3 nm are used,the backward SBS reflectivities show novel behavior in which beams of longer wavelength achieve higher SBS gain.This property of SBS can be attributed to the rotation of the wave vectors of common ion acoustic waves due to the competition of detunings between geometrical angle and wavelength separation.This mechanism is confirmed using massively parallel supercomputer simulations with the three-dimensional laser–plasma interaction code LAP3D.
基金financially supported by the Foundation of State Key Laboratory of Safety and Health for Metal Mines(2018-JSKSSYS-05).
文摘In this research,the dynamics of wet spray nozzles with different geometries,used to accelerate shotcrete,are investigated on the basis of a suitable three-dimensional mathematical model and related numerical method.Simulations have been conducted in the frame of the SIMPLEC algorithm.The k-εturbulence model has been used to account for turbulent effects.The study shows that when the angle of the convergent section is less than 3°,it has a scarce effect on the dynamics of the jet of shotcrete;with the increase of the convergence angle,the shotcrete jet velocity decreases and the nozzle wear increases;when this angle is greater than 6°,the concrete outlet jet velocity is very small and the nozzle can easily be blocked.Experimental results are in good agreement with the outcomes of the numerical simulations,which indicates that the used approach is reliable.
基金Zhejiang Provincial Key Research Project(2014C03022).
文摘A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contaminants in DPW,which was pretreated with a membrane bioreactor(MBR).The strain was identified as Arthrospira platensis(A.platensis)based on its morphological characteristics and the 16S rDNA sequencing analysis.The effect of chrominance on growth of A.platensis ZJWST-S1 was investigated in a field raceway pond filled with MBR effluent or MBR effluent decolorized with ozonation.After ozonation,the chrominance of MBR effluent was decreased from 700 mg Pt/L to 150 mg Pt/L.Two runs of cultivation showed that A.platensis ZJWST-S1 grew faster in the ozone decolorized MBR effluent,the averaged biomass concentration being 0.907 g/L after 10 days of cultivation,close to that in a Zarrouk medium(0.969 g/L).By comparison,the biomass grew much slower in the non-decolorized MBR effluent(0.624 g/L).The pollutant removal was also benefited from the accelerative growth of A.platensis ZJWST-S1 in the decolorized MBR effluent.Almost all ammonium,61.2%of nitrate and 68.1%of phosphate were removed by the A.platensis ZJWST-S1 in the decolorized MBR effluent,much higher than the corresponding values of almost all ammonium,25.4%of nitrate and 36.5%of phosphate in the MBR effluent.Furthermore,the Arthrospira biomass harvested from the ozone decolorized MBR effluent after 10 d cultivation was with crude protein content of 59.1%±3.5%in dry algae powder.The content of Pb,As,Cd and Hg in biomass was also low enough to meet the Chinese Arthrospira Standard for Animal Feed(GB/T 17243-1998).This study showed that the new strain A.platensis ZJWST-S1 possessed potential to be used for producing animal feed and simultaneous removal of nitrogen and phosphorus in DPW.
