Aim: To investigate a simple method for assaying acrosin activity for the evaluation of male fertility. Methods:The acrosin activity of 7.5 × 10~6 sperm without seminal plasma and acrosin activity inhibitors was ...Aim: To investigate a simple method for assaying acrosin activity for the evaluation of male fertility. Methods:The acrosin activity of 7.5 × 10~6 sperm without seminal plasma and acrosin activity inhibitors was assayed using N-α-benzoyl-DL-arginine-p-nitroanilide (BAPNA) and detergent (Triton X-100) as substrate. Results: The acrosin ac-tivity of 60 normal fertile men (35 ± 10 μIU/10~6 sperm ) was higher than that of 168 infertile men ( 16 ± 8 μIU/10~6sperm) (P <0.01). It was indicated that there was a significant positive correlation between the acrosin activity andthe sperm motility ( r ≥ 0.6534, P < 0.01) and a significant negative correlation between the sperm malformed rateand the WBC number ( r ≤ -0.5426, P < 0.01). The temperature and time of incubation and the sperm concentrationcould influence the assay results. Conclusion: Acrosin activity is an important index for the evaluation of male fer-tility. The approach developed by the authors is a simple method for the determination of acrosin activity.展开更多
AIM: To detect the expression of Fas ligand (FasL) in colon cancer tissues and cell lines and analyze the function of FasL-expressing colon cancer cells in inducing Fas-sensitive T lymphocyte apoptosis. METHODS: Ninet...AIM: To detect the expression of Fas ligand (FasL) in colon cancer tissues and cell lines and analyze the function of FasL-expressing colon cancer cells in inducing Fas-sensitive T lymphocyte apoptosis. METHODS: Ninety surgically resected colon cancer tissues and 15 hepatic metastasis specimens were investigatedby immunohistochemical method with normal colon mucosa and colon adenoma as control. The relationship between FasL expression and pathologic features was also analyzed.FasL expression of 4 colon cancer cell lines, SW620, Lovo, LS-174T and SW1116, were detected by Western blotting assay. The function of FasL expressed on colon cancer cells was determined by coculture assay with Jurkat T lymphocytes, the apoptotic rate of which was detectedby flow cytometry assay.RESULTS: Fifty-six (62.22%) cases of all the 90 colon cancer tissues and all (100%) the liver metastasis specimens expressed FasL, significantly higher than normal colon mucosa and colonic adenoma. Higher expression of FasL was found in more advanced stage of colon cancer and in cancer tissues with lymphatic or hepatic metastasis.All the colon cancer cell lines were found to express FasL.After coculture with the SW1116 cells for 24 h with an effector: target ratio 10:1, the rate of apoptosis of Jurkat cells rose from 1.9% to 21.0%.CONCLUSION: The expression of FasL is upregulated in colon cancer and the functionally expressed FasL can induce apoptosis of Fas-expressing T lymphocytes.展开更多
Aim: To study the effect of smoking on the semen quality in infertile men in Shandong Province, China. Methods:Adult non-drinker males attending the infertility clinic, including 110 non-smokers and 191 smokers, were ...Aim: To study the effect of smoking on the semen quality in infertile men in Shandong Province, China. Methods:Adult non-drinker males attending the infertility clinic, including 110 non-smokers and 191 smokers, were recruited forthe study. Sixty-one fertile, non-smoker and non-drinker males, who had one or more children, served as the controls.The smokers were divided into subgroups according to the amount and duration of smoking. Semen parameters (semenvolume and sperm density, viability, motility, and morphology) were examined and seminal plasma contents of Zn,Cu and superoxide dismutase (SOD) determined. Results: The semen volume and acidity, and the sperm density,viability and forward progression, as well as the seminal plasma contents of Zn, Cu and SOD were much lower in themedium, heavy and long-term smokers than in the non-smokers ( P < 0.01). The sperm density, viability and forwardprogression, and the seminal plasma Zn, Cu and SOD levels were negatively correlated with the amount and duration ofcigarette smoking (P < 0.01). Conclusion: Medium, heavy and long-term smoking adversely affected the semenquality in a population of men visiting the infertility clinic in Shandong, China.展开更多
Compression waves propagating through molten metals are contributed to degassing, accelerating reaction rate,removing exclusions from molten metals and refining solidification structures during metallurgical processin...Compression waves propagating through molten metals are contributed to degassing, accelerating reaction rate,removing exclusions from molten metals and refining solidification structures during metallurgical processing of materials. In the present study, two electromagnetic methods are proposed to generate intense compression wavesdirectly in liquid metals. One is the simultaneous imposition of a high frequency electrical current field and a staticmagnetic field; the other is that of a high frequency magnetic field and a static magnetic field. A mathematical modelbased on compressible fluid dynamics and electromagnetic fields theory has been developed to derive pressure distributions of the generated waves in a metal. It shows that the intensity of compression waves is proportional to thatof the high frequency electromagnetic force. And the frequency is the same as that of the imposed electromagneticforce. On the basis of theoretical analyses, pressure change in liquid gallium was examined by a pressure transducerunder various conditions. The observed results approximately agreed with the predictions derived from the theoreticalanalyses and calculations. Moreover, the effect of the generated waves on improvement of solidification structureswas also examined. It shows that the generated compression waves can refine solidification structures when they wereapplied to solidification process of Sn-Pb alloy. This study indicates a new method to generate compression wavesby imposing high frequency electromagnetic force locally on molten metals and this kind of compression waves canprobably overcome the difficulties when waves are excited by mechanical vibration in high temperature environments.展开更多
Research on microcapsules has been conducted in recent years given trends in miniaturization and novel functionalization.In this work,we designed and prepared a series of unique shape memory polyurethane(SMPU)microcap...Research on microcapsules has been conducted in recent years given trends in miniaturization and novel functionalization.In this work,we designed and prepared a series of unique shape memory polyurethane(SMPU)microcapsules with stimuli-responsive func-tions.The microcapsule has a core-shell structure in which the surface morphology can be adjusted,and it has a certain loadbearing capacity.In addition,the SMPU microcapsule has a stimuliresponsive function for shape memory and solvent response.The temperature of its shape recovery is approximately body tempera-ture,and it can swell to rupture under the stimulation of organic solvents.Thus,the SMPU microcapsule has potential applications in biomedical fields,such as drug release.展开更多
To clone and identify the proteins involved in regulating the transcription of hTERT and study the role of genes in both hTERT transcription and telomerase activity. Methods The full cDNA of COUP-TFII was clon...To clone and identify the proteins involved in regulating the transcription of hTERT and study the role of genes in both hTERT transcription and telomerase activity. Methods The full cDNA of COUP-TFII was cloned from HeLa cDNA library by hTERT promoter-based yeast one-hybrid assay and then in-frame inserted into His-tag fusion expression vector pEK318. The His-tag COUP-TFII fusion proteins were purified by Ni-NTA chromatography. The interaction of COUP-TFII with hTERT promoter in vitro was identified by electrophoretic mobility shift assay and Footprint. The role of COUP-TFII in both hTERT transcription and telomerase activity were probed through Luciferase reporter assay, Northern blot, and TRAP-PCR ELISA. Results COUP-TFII could firmly bind to the downstream E-box and the other two binding sites in hTERT promoter. Luciferase reporter assay indicated COUP-TFII could suppress hTERT promoter activity and stable introduction of COUP-TFII into HeLa cells also decreased both endogenous hTERT transcription and telomerase activity. Conclusion The human COUP-TFII can firmly bind to hTERT promoter, and inhibit telomerase activity through decreasing hTERT transcription. It will greatly facilitate understanding of telomerase regulation in normal and cancer cells展开更多
The oxidation of a,b-unsaturated primary and secondary alcohols to corresponding aldehydes and ketones by manganese dioxide in ionic liquids as a safe recyclable and accelerative reaction medium under mild conditions ...The oxidation of a,b-unsaturated primary and secondary alcohols to corresponding aldehydes and ketones by manganese dioxide in ionic liquids as a safe recyclable and accelerative reaction medium under mild conditions are described. The rate of the oxidation reaction is faster and the yield is higher than that with conventional procedures.展开更多
The marginal sea and back-arc basins in the Western Pacific Ocean have become the focus of tectonics due to their unique tectonic location.To understand the deep crustal structure in the back-arc region,we present a 5...The marginal sea and back-arc basins in the Western Pacific Ocean have become the focus of tectonics due to their unique tectonic location.To understand the deep crustal structure in the back-arc region,we present a 545-kmlong active-source ocean bottom seismometer(OBS)wide-angle reflection/refraction profile in the East China Sea.The P wave velocity model shows that the Moho depth rises significantly,from approximately 30 km in the East China Sea shelf to approximately 16 km in the axis of the Okinawa Trough.The lower crustal high-velocity zone(HVZ)in the southern Okinawa Trough,with V_(p) of 6.8-7.3 km/s,is a remarkable manifestation of the mantle material upwelling and accretion to the lower crust.This confirms that the lower crustal high-velocity mantle accretion is developed in the southern Okinawa Trough.During the process of back-arc extension,the crustal structure of the southern Okinawa Trough is completely invaded and penetrated by the upper mantle material in the axis region.In some areas of the southern central graben,the crust may has broken up and entered the initial stage of seafloor spreading.The discontinuous HVZs in the lower crust in the back-arc region also indicate the migration of spreading centers in the back-arc region since the Cenozoic.The asthenosphere material upwelling in the continent-ocean transition zone is constantly driving the lithosphere eastward for episodic extension,and is causing evident tectonic migration in the Western Pacific back-arc region.展开更多
A new designed surface strengthening method, surface spinning strengthening （3S）, was applied to achieve gradient microstructure in the surface layer of a Cu-11 at.%A1 alloy. According to the level of grain refineme...A new designed surface strengthening method, surface spinning strengthening （3S）, was applied to achieve gradient microstructure in the surface layer of a Cu-11 at.%A1 alloy. According to the level of grain refinement, the gradient microstructure can be divided into four zones, including nanoscale grain zone, ultra-fine grain zone, fine grain zone and coarse grain zone from the surface to the matrix. Meanwhile, a plenty of grain boundaries and twin boundaries were introduced to inhibit the dislocation motion in the surface layer during the plastic deformation process. Consequently, the hardened layer with a microhardness gradient and high residual compressive stress was produced on the samples, and the yield strength of the Cu-11 at.%A1 alloy was effectively improved after 3S processing due to the strengthening effect caused by the gradient microstructurc.展开更多
Understanding and modulating the interaction between various reactive molecules and oxygen carriers are the key issue to achieve process intensification of chemical looping technology.C1 chemical molecules play an imp...Understanding and modulating the interaction between various reactive molecules and oxygen carriers are the key issue to achieve process intensification of chemical looping technology.C1 chemical molecules play an important role in many reactions involved with chemical looping processes.However,up to now,there is still a lack of systematic and in-depth understanding of the adsorption mechanism of C1 molecules on the surface of oxygen carriers(OCs).In this work,the intrinsic interaction between a series of C1 molecules composed of CH4,CO,CO2,CH3OH,HCHO and HCOOH and surface of Ni O OCs in the chemical looping process have been studied using density functional theory calculations.Various adsorption configurations of C1 molecules and also different adsorption sites of Ni O have been considered.The structural features of stable configuration of C1 molecules on the surface of NiO OCs have been obtained.Further,the interacted sites,types and strengths of C1 molecules on the surface of NiO have been directly pictured by the independent gradient model methods.Also,the nature of the interaction between C1 molecule and Ni O surface has been investigated with the aid of energy decomposition analysis from a quantitative view.展开更多
OBJECTIVE To summarize the experience in diagnosing and treating parathyroid adenoma.METHODS Twenty-four patients were diagnosed with parathyroid adenoma and received parathyroidectomy in our hospital. Sixteen of them...OBJECTIVE To summarize the experience in diagnosing and treating parathyroid adenoma.METHODS Twenty-four patients were diagnosed with parathyroid adenoma and received parathyroidectomy in our hospital. Sixteen of them presented with hyperparathyroidism. The patients received ultrasounography, CT or ^99mTc-MIBI to locate the tumor site. Serum concentrations of PTH and calcium were checked before the operation. All operations were performed under general anesthesia. The adenomas were resected and the four glands explored.RESULTS All of the patients were cured and there was no mortality in our group. The symptoms of hyperparathyroidism remitted to various degrees after the operation. PTH dropped to the normal range 2 days after operation.Serum calcium concentrations declined to different levels from the first day after operation. Seven patients developed hypocalcemia post-operation but recovered by injection of calcium gluconate. Only one of the patients with parathyroid adenoma recurred 2 years after the operation and was found to have malignancy of the parathyroid adenoma.CONCLUSION Not all the patients with parathyroid adenoma had clinical manifestations. The CT and ^99mTc-MIBI were more accurate than ultrasounography in locating the adenoma. The four glands should be explored during the operation. Protecting the recurrent laryngeal nerve from being injuried and maintaning secure hemastasis were most important.展开更多
This paper describes a novel method for tracking complex non-rigid motions bylearning the intrinsic object structure. The approach builds on and extends the studies onnon-linear dimensionality reduction for object rep...This paper describes a novel method for tracking complex non-rigid motions bylearning the intrinsic object structure. The approach builds on and extends the studies onnon-linear dimensionality reduction for object representation, object dynamics modeling and particlefilter style tracking. First, the dimensionality reduction and density estimation algorithm isderived for unsupervised learning of object intrinsic representation, and the obtained non-rigidpart of object state reduces even to 2-3 dimensions. Secondly the dynamical model is derived andtrained based on this intrinsic representation. Thirdly the learned intrinsic object structure isintegrated into a particle filter style tracker. It is shown that this intrinsic objectrepresentation has some interesting properties and based on which the newly derived dynamical modelmakes particle filter style tracker more robust and reliable. Extensive experiments are done on thetracking of challenging non-rigid motions such as fish twisting with self-occlusion, largeinter-frame lip motion and facial expressions with global head rotation. Quantitative results aregiven to make comparisons between the newly proposed tracker and the existing tracker. The proposedmethod also has the potential to solve other type of tracking problems.展开更多
We will present the concept and definition about software architecture and software reuse. And then discuss the software architecture that can be reused. Finally we present a demo of reusable software system in a MIS ...We will present the concept and definition about software architecture and software reuse. And then discuss the software architecture that can be reused. Finally we present a demo of reusable software system in a MIS project.展开更多
Due to its controlled reaction with water and biofluids, Mg as a dissolvable conductor has enabled the development of many transient electronic devices. In addition, Mg is a novel plasmonic material with high extincti...Due to its controlled reaction with water and biofluids, Mg as a dissolvable conductor has enabled the development of many transient electronic devices. In addition, Mg is a novel plasmonic material with high extinction efficiency but its transient optical properties have not been explored thoroughly. In this study for the first time, we exploit the transient and tunable plasmonic properties of Mg in environmental and biomedical sensor applications. We used soft nanoimprint lithography to fabricate flexible and large-area Mg plasmonic structures that can be applied on the human skin. Their resonance （or color） can be tuned in the visible range by gradual Mg dissolution in a water fluid or vapor-rich environment; these structures can be easily implemented as passive optical sensors without the need for complex electronic circuits or a power supply. We demonstrate the applications of our optical sensors in the accurate monitoring of environmental humidity and physiological detection of sweat loss on the human skin during exercise. Our devices could be used as decomposable/resorbable optical sensors and can help minimize long-term health effects and environmental risks associated with consumer device waste, which will lead to many new possibilities in transient photonic device applications.展开更多
AIM: To study and compare the difference of activationinduced cell death (AICD) in peripheral blood T-lymphocytes (PBL-Ts) from patients with chronic hepatitis B (CHB) and the normal people in vitro, and to explore th...AIM: To study and compare the difference of activationinduced cell death (AICD) in peripheral blood T-lymphocytes (PBL-Ts) from patients with chronic hepatitis B (CHB) and the normal people in vitro, and to explore the role of AICD in chronic hepatitis B virus (HBV) infection and the pathogenesis of CHB.METHODS: Twenty-five patients and fourteen healthy people were selected for isolation of PBL-Ts. During cultivation, antiCD3 mAb, PMA and ionomycin were used for AICD of PBL-Ts.AICD ratio of PBL-Ts was detected with TdT-mediated dUTP nick end labeling and assessed by flow cytometry.RESULTS: When induced with anti-CD3, PMA and ionomycin in vitro, AICD ratio of PBL-Ts from CHB patients was significantly higher than that from healthy control (17.24±1.21VS. 6.63±1.00, P＜0.01) and that from CHB patients without induction (17.24±1.21 VS. 9.88±1.36, P＜0.0L). There was a similar AICD ratio of PBL-Ts between induction group and without induction group, but no difference was found before and after induction in healthy control. The density of INF-γ in culture media of induction groups of CHB was lower than that of other groups (P＜0.01). There was no difference between these groups in density of IL-10 (P＞0.05).CONCLUSION: When induced during cultivation in vitro,PBL-Ts from CHB have AICD very commonly. This phenomenon has a potentially important relation with pathogenesis of CHB and chronicity of HBV infection.展开更多
Background： It is important to achieve the definitive pathogen identification in hospital-acquired pneumonia （HAP）, but the traditional culture results always delay the target antibiotic therapy. We assessed the me...Background： It is important to achieve the definitive pathogen identification in hospital-acquired pneumonia （HAP）, but the traditional culture results always delay the target antibiotic therapy. We assessed the method called quantitative loop-mediated isothermal amplification （qLAMP） as a new implement for steering of the antibiotic decision-making in HAP. Methods： Totally, 76 respiratory tract aspiration samples were prospectively collected from 60 HAP patients. DNA was isolated from these samples. Specific DNA fragments for identifying 11 pneumonia-related bacteria were amplified by qLAMP assay. Culture results of these patients were compared with the qLAMP results. Clinical data and treatment strategies were analyzed to evaluate the effects of qLAMP results on clinical data. McNemar test and Fisher＇s exact test were used for statistical analysis. Results： The detection of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Stenotrophomonas maltophilia, Streptococcus pneumonia, and Acinetobacter baumannii by qLAMP was consistent with sputum culture （P 〉 0.05）. The qLAMP results of 4 samples for Haemophilus influenzae, Legionella pneumophila, or Mvcoplasma pneumonia （MP） were inconsistent with culture results; however, clinical data revealed that the qLAMP results were all reliable except 1 MP positive sample due to the lack of specific species identified in the final diagnosis. The improvement of clinical condition was more significant （P 〈 0.001） in patients with pathogen target-driven therapy based on qLAMP results than those with empirical therapy. Conclusion： qLAMP is a more promising method for detection of pathogens in an early, rapid, sensitive, and specific manner than culture.展开更多
文摘Aim: To investigate a simple method for assaying acrosin activity for the evaluation of male fertility. Methods:The acrosin activity of 7.5 × 10~6 sperm without seminal plasma and acrosin activity inhibitors was assayed using N-α-benzoyl-DL-arginine-p-nitroanilide (BAPNA) and detergent (Triton X-100) as substrate. Results: The acrosin ac-tivity of 60 normal fertile men (35 ± 10 μIU/10~6 sperm ) was higher than that of 168 infertile men ( 16 ± 8 μIU/10~6sperm) (P <0.01). It was indicated that there was a significant positive correlation between the acrosin activity andthe sperm motility ( r ≥ 0.6534, P < 0.01) and a significant negative correlation between the sperm malformed rateand the WBC number ( r ≤ -0.5426, P < 0.01). The temperature and time of incubation and the sperm concentrationcould influence the assay results. Conclusion: Acrosin activity is an important index for the evaluation of male fer-tility. The approach developed by the authors is a simple method for the determination of acrosin activity.
基金Supported by the National Natural Science Foundation of China,No.39900143
文摘AIM: To detect the expression of Fas ligand (FasL) in colon cancer tissues and cell lines and analyze the function of FasL-expressing colon cancer cells in inducing Fas-sensitive T lymphocyte apoptosis. METHODS: Ninety surgically resected colon cancer tissues and 15 hepatic metastasis specimens were investigatedby immunohistochemical method with normal colon mucosa and colon adenoma as control. The relationship between FasL expression and pathologic features was also analyzed.FasL expression of 4 colon cancer cell lines, SW620, Lovo, LS-174T and SW1116, were detected by Western blotting assay. The function of FasL expressed on colon cancer cells was determined by coculture assay with Jurkat T lymphocytes, the apoptotic rate of which was detectedby flow cytometry assay.RESULTS: Fifty-six (62.22%) cases of all the 90 colon cancer tissues and all (100%) the liver metastasis specimens expressed FasL, significantly higher than normal colon mucosa and colonic adenoma. Higher expression of FasL was found in more advanced stage of colon cancer and in cancer tissues with lymphatic or hepatic metastasis.All the colon cancer cell lines were found to express FasL.After coculture with the SW1116 cells for 24 h with an effector: target ratio 10:1, the rate of apoptosis of Jurkat cells rose from 1.9% to 21.0%.CONCLUSION: The expression of FasL is upregulated in colon cancer and the functionally expressed FasL can induce apoptosis of Fas-expressing T lymphocytes.
文摘Aim: To study the effect of smoking on the semen quality in infertile men in Shandong Province, China. Methods:Adult non-drinker males attending the infertility clinic, including 110 non-smokers and 191 smokers, were recruited forthe study. Sixty-one fertile, non-smoker and non-drinker males, who had one or more children, served as the controls.The smokers were divided into subgroups according to the amount and duration of smoking. Semen parameters (semenvolume and sperm density, viability, motility, and morphology) were examined and seminal plasma contents of Zn,Cu and superoxide dismutase (SOD) determined. Results: The semen volume and acidity, and the sperm density,viability and forward progression, as well as the seminal plasma contents of Zn, Cu and SOD were much lower in themedium, heavy and long-term smokers than in the non-smokers ( P < 0.01). The sperm density, viability and forwardprogression, and the seminal plasma Zn, Cu and SOD levels were negatively correlated with the amount and duration ofcigarette smoking (P < 0.01). Conclusion: Medium, heavy and long-term smoking adversely affected the semenquality in a population of men visiting the infertility clinic in Shandong, China.
基金This work has been partially performed under the financial supports from the National Natural Science Foundation of China (Grant No.50204004)the National Fundamental Research Project - new generation steel research project of China (Grant No.G1998061
文摘Compression waves propagating through molten metals are contributed to degassing, accelerating reaction rate,removing exclusions from molten metals and refining solidification structures during metallurgical processing of materials. In the present study, two electromagnetic methods are proposed to generate intense compression wavesdirectly in liquid metals. One is the simultaneous imposition of a high frequency electrical current field and a staticmagnetic field; the other is that of a high frequency magnetic field and a static magnetic field. A mathematical modelbased on compressible fluid dynamics and electromagnetic fields theory has been developed to derive pressure distributions of the generated waves in a metal. It shows that the intensity of compression waves is proportional to thatof the high frequency electromagnetic force. And the frequency is the same as that of the imposed electromagneticforce. On the basis of theoretical analyses, pressure change in liquid gallium was examined by a pressure transducerunder various conditions. The observed results approximately agreed with the predictions derived from the theoreticalanalyses and calculations. Moreover, the effect of the generated waves on improvement of solidification structureswas also examined. It shows that the generated compression waves can refine solidification structures when they wereapplied to solidification process of Sn-Pb alloy. This study indicates a new method to generate compression wavesby imposing high frequency electromagnetic force locally on molten metals and this kind of compression waves canprobably overcome the difficulties when waves are excited by mechanical vibration in high temperature environments.
基金the National Natural Science Foundation of China(Grant No.11802075,12072094).
文摘Research on microcapsules has been conducted in recent years given trends in miniaturization and novel functionalization.In this work,we designed and prepared a series of unique shape memory polyurethane(SMPU)microcapsules with stimuli-responsive func-tions.The microcapsule has a core-shell structure in which the surface morphology can be adjusted,and it has a certain loadbearing capacity.In addition,the SMPU microcapsule has a stimuliresponsive function for shape memory and solvent response.The temperature of its shape recovery is approximately body tempera-ture,and it can swell to rupture under the stimulation of organic solvents.Thus,the SMPU microcapsule has potential applications in biomedical fields,such as drug release.
文摘To clone and identify the proteins involved in regulating the transcription of hTERT and study the role of genes in both hTERT transcription and telomerase activity. Methods The full cDNA of COUP-TFII was cloned from HeLa cDNA library by hTERT promoter-based yeast one-hybrid assay and then in-frame inserted into His-tag fusion expression vector pEK318. The His-tag COUP-TFII fusion proteins were purified by Ni-NTA chromatography. The interaction of COUP-TFII with hTERT promoter in vitro was identified by electrophoretic mobility shift assay and Footprint. The role of COUP-TFII in both hTERT transcription and telomerase activity were probed through Luciferase reporter assay, Northern blot, and TRAP-PCR ELISA. Results COUP-TFII could firmly bind to the downstream E-box and the other two binding sites in hTERT promoter. Luciferase reporter assay indicated COUP-TFII could suppress hTERT promoter activity and stable introduction of COUP-TFII into HeLa cells also decreased both endogenous hTERT transcription and telomerase activity. Conclusion The human COUP-TFII can firmly bind to hTERT promoter, and inhibit telomerase activity through decreasing hTERT transcription. It will greatly facilitate understanding of telomerase regulation in normal and cancer cells
文摘The oxidation of a,b-unsaturated primary and secondary alcohols to corresponding aldehydes and ketones by manganese dioxide in ionic liquids as a safe recyclable and accelerative reaction medium under mild conditions are described. The rate of the oxidation reaction is faster and the yield is higher than that with conventional procedures.
基金supported by the National Key Basic Research Program of China(Grant No.2013CB429701)the National Natural Science Foundation of China(Grant Nos.41606083,91958210,41606050 and 41210005)+1 种基金AoShan Technological Innovation Projects of National Laboratory for Marine Science and Technology(Qingdao)(2015ASKJ03)National Marine Geological Special Project(DD20190236,DD20190365,DD20190377)。
文摘The marginal sea and back-arc basins in the Western Pacific Ocean have become the focus of tectonics due to their unique tectonic location.To understand the deep crustal structure in the back-arc region,we present a 545-kmlong active-source ocean bottom seismometer(OBS)wide-angle reflection/refraction profile in the East China Sea.The P wave velocity model shows that the Moho depth rises significantly,from approximately 30 km in the East China Sea shelf to approximately 16 km in the axis of the Okinawa Trough.The lower crustal high-velocity zone(HVZ)in the southern Okinawa Trough,with V_(p) of 6.8-7.3 km/s,is a remarkable manifestation of the mantle material upwelling and accretion to the lower crust.This confirms that the lower crustal high-velocity mantle accretion is developed in the southern Okinawa Trough.During the process of back-arc extension,the crustal structure of the southern Okinawa Trough is completely invaded and penetrated by the upper mantle material in the axis region.In some areas of the southern central graben,the crust may has broken up and entered the initial stage of seafloor spreading.The discontinuous HVZs in the lower crust in the back-arc region also indicate the migration of spreading centers in the back-arc region since the Cenozoic.The asthenosphere material upwelling in the continent-ocean transition zone is constantly driving the lithosphere eastward for episodic extension,and is causing evident tectonic migration in the Western Pacific back-arc region.
基金financially supported by the National Natural Science Foundation of China(NSFC) under Grant Nos.51331007 and 51501198
文摘A new designed surface strengthening method, surface spinning strengthening （3S）, was applied to achieve gradient microstructure in the surface layer of a Cu-11 at.%A1 alloy. According to the level of grain refinement, the gradient microstructure can be divided into four zones, including nanoscale grain zone, ultra-fine grain zone, fine grain zone and coarse grain zone from the surface to the matrix. Meanwhile, a plenty of grain boundaries and twin boundaries were introduced to inhibit the dislocation motion in the surface layer during the plastic deformation process. Consequently, the hardened layer with a microhardness gradient and high residual compressive stress was produced on the samples, and the yield strength of the Cu-11 at.%A1 alloy was effectively improved after 3S processing due to the strengthening effect caused by the gradient microstructurc.
基金supported by Natural Science Founda tion of Ningxia(No.2020AAC03018)financial supports from Key R&D Projects of Ningxia(No.2018BCE01002)National Academic Subjects Construction Project of Ningxia(Chemical Engineering and Technology,NXYLXK2017A04)。
文摘Understanding and modulating the interaction between various reactive molecules and oxygen carriers are the key issue to achieve process intensification of chemical looping technology.C1 chemical molecules play an important role in many reactions involved with chemical looping processes.However,up to now,there is still a lack of systematic and in-depth understanding of the adsorption mechanism of C1 molecules on the surface of oxygen carriers(OCs).In this work,the intrinsic interaction between a series of C1 molecules composed of CH4,CO,CO2,CH3OH,HCHO and HCOOH and surface of Ni O OCs in the chemical looping process have been studied using density functional theory calculations.Various adsorption configurations of C1 molecules and also different adsorption sites of Ni O have been considered.The structural features of stable configuration of C1 molecules on the surface of NiO OCs have been obtained.Further,the interacted sites,types and strengths of C1 molecules on the surface of NiO have been directly pictured by the independent gradient model methods.Also,the nature of the interaction between C1 molecule and Ni O surface has been investigated with the aid of energy decomposition analysis from a quantitative view.
文摘OBJECTIVE To summarize the experience in diagnosing and treating parathyroid adenoma.METHODS Twenty-four patients were diagnosed with parathyroid adenoma and received parathyroidectomy in our hospital. Sixteen of them presented with hyperparathyroidism. The patients received ultrasounography, CT or ^99mTc-MIBI to locate the tumor site. Serum concentrations of PTH and calcium were checked before the operation. All operations were performed under general anesthesia. The adenomas were resected and the four glands explored.RESULTS All of the patients were cured and there was no mortality in our group. The symptoms of hyperparathyroidism remitted to various degrees after the operation. PTH dropped to the normal range 2 days after operation.Serum calcium concentrations declined to different levels from the first day after operation. Seven patients developed hypocalcemia post-operation but recovered by injection of calcium gluconate. Only one of the patients with parathyroid adenoma recurred 2 years after the operation and was found to have malignancy of the parathyroid adenoma.CONCLUSION Not all the patients with parathyroid adenoma had clinical manifestations. The CT and ^99mTc-MIBI were more accurate than ultrasounography in locating the adenoma. The four glands should be explored during the operation. Protecting the recurrent laryngeal nerve from being injuried and maintaning secure hemastasis were most important.
文摘This paper describes a novel method for tracking complex non-rigid motions bylearning the intrinsic object structure. The approach builds on and extends the studies onnon-linear dimensionality reduction for object representation, object dynamics modeling and particlefilter style tracking. First, the dimensionality reduction and density estimation algorithm isderived for unsupervised learning of object intrinsic representation, and the obtained non-rigidpart of object state reduces even to 2-3 dimensions. Secondly the dynamical model is derived andtrained based on this intrinsic representation. Thirdly the learned intrinsic object structure isintegrated into a particle filter style tracker. It is shown that this intrinsic objectrepresentation has some interesting properties and based on which the newly derived dynamical modelmakes particle filter style tracker more robust and reliable. Extensive experiments are done on thetracking of challenging non-rigid motions such as fish twisting with self-occlusion, largeinter-frame lip motion and facial expressions with global head rotation. Quantitative results aregiven to make comparisons between the newly proposed tracker and the existing tracker. The proposedmethod also has the potential to solve other type of tracking problems.
文摘We will present the concept and definition about software architecture and software reuse. And then discuss the software architecture that can be reused. Finally we present a demo of reusable software system in a MIS project.
文摘Due to its controlled reaction with water and biofluids, Mg as a dissolvable conductor has enabled the development of many transient electronic devices. In addition, Mg is a novel plasmonic material with high extinction efficiency but its transient optical properties have not been explored thoroughly. In this study for the first time, we exploit the transient and tunable plasmonic properties of Mg in environmental and biomedical sensor applications. We used soft nanoimprint lithography to fabricate flexible and large-area Mg plasmonic structures that can be applied on the human skin. Their resonance （or color） can be tuned in the visible range by gradual Mg dissolution in a water fluid or vapor-rich environment; these structures can be easily implemented as passive optical sensors without the need for complex electronic circuits or a power supply. We demonstrate the applications of our optical sensors in the accurate monitoring of environmental humidity and physiological detection of sweat loss on the human skin during exercise. Our devices could be used as decomposable/resorbable optical sensors and can help minimize long-term health effects and environmental risks associated with consumer device waste, which will lead to many new possibilities in transient photonic device applications.
基金the National Natural Science Foundation of China,No.39570655
文摘AIM: To study and compare the difference of activationinduced cell death (AICD) in peripheral blood T-lymphocytes (PBL-Ts) from patients with chronic hepatitis B (CHB) and the normal people in vitro, and to explore the role of AICD in chronic hepatitis B virus (HBV) infection and the pathogenesis of CHB.METHODS: Twenty-five patients and fourteen healthy people were selected for isolation of PBL-Ts. During cultivation, antiCD3 mAb, PMA and ionomycin were used for AICD of PBL-Ts.AICD ratio of PBL-Ts was detected with TdT-mediated dUTP nick end labeling and assessed by flow cytometry.RESULTS: When induced with anti-CD3, PMA and ionomycin in vitro, AICD ratio of PBL-Ts from CHB patients was significantly higher than that from healthy control (17.24±1.21VS. 6.63±1.00, P＜0.01) and that from CHB patients without induction (17.24±1.21 VS. 9.88±1.36, P＜0.0L). There was a similar AICD ratio of PBL-Ts between induction group and without induction group, but no difference was found before and after induction in healthy control. The density of INF-γ in culture media of induction groups of CHB was lower than that of other groups (P＜0.01). There was no difference between these groups in density of IL-10 (P＞0.05).CONCLUSION: When induced during cultivation in vitro,PBL-Ts from CHB have AICD very commonly. This phenomenon has a potentially important relation with pathogenesis of CHB and chronicity of HBV infection.
文摘Background： It is important to achieve the definitive pathogen identification in hospital-acquired pneumonia （HAP）, but the traditional culture results always delay the target antibiotic therapy. We assessed the method called quantitative loop-mediated isothermal amplification （qLAMP） as a new implement for steering of the antibiotic decision-making in HAP. Methods： Totally, 76 respiratory tract aspiration samples were prospectively collected from 60 HAP patients. DNA was isolated from these samples. Specific DNA fragments for identifying 11 pneumonia-related bacteria were amplified by qLAMP assay. Culture results of these patients were compared with the qLAMP results. Clinical data and treatment strategies were analyzed to evaluate the effects of qLAMP results on clinical data. McNemar test and Fisher＇s exact test were used for statistical analysis. Results： The detection of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Stenotrophomonas maltophilia, Streptococcus pneumonia, and Acinetobacter baumannii by qLAMP was consistent with sputum culture （P 〉 0.05）. The qLAMP results of 4 samples for Haemophilus influenzae, Legionella pneumophila, or Mvcoplasma pneumonia （MP） were inconsistent with culture results; however, clinical data revealed that the qLAMP results were all reliable except 1 MP positive sample due to the lack of specific species identified in the final diagnosis. The improvement of clinical condition was more significant （P 〈 0.001） in patients with pathogen target-driven therapy based on qLAMP results than those with empirical therapy. Conclusion： qLAMP is a more promising method for detection of pathogens in an early, rapid, sensitive, and specific manner than culture.
