Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of ba...Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of baicalin in a neonatal rat model of hypoxic-ischemic encephalopathy.Seven-day-old pups underwent left common carotid artery ligation followed by hypoxia（8% oxygen at 37°C） for 2 hours,before being injected with baicalin（120 mg/kg intraperitoneally） and examined 24 hours later.Baicalin effectively reduced cerebral infarct volume and neuronal loss,inhibited apoptosis,and upregulated the expression of p-Akt and glutamate transporter 1.Intracerebroventricular injection of the phosphoinositide 3-kinase/protein kinase B（PI3 K/Akt） inhibitor LY294002 30 minutes before injury blocked the effect of baicalin on p-Akt and glutamate transporter 1,and weakened the associated neuroprotective effect.Our findings provide the first evidence,to our knowledge that baicalin can protect neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the PI3 K/Akt signaling pathway.展开更多
Biomedical polymers have been extensively developed for promising applications in a lot of biomedical fields, such as therapeutic medicine delivery, disease detection and diagnosis, biosensing, regenerative medicine, ...Biomedical polymers have been extensively developed for promising applications in a lot of biomedical fields, such as therapeutic medicine delivery, disease detection and diagnosis, biosensing, regenerative medicine, and disease treatment. In this review, we summarize the most recent advances in the synthesis and application of biomedical polymers, and discuss the comprehensive understanding of their property-function relationship for corresponding biomedical applications. In particular, a few burgeoning bioactive polymers, such as peptide/biomembrane/microorganism/cell-based biomedical polymers, are also introduced and highlighted as the emerging biomaterials for cancer precision therapy. Furthermore, the foreseeable challenges and outlook of the development of more efficient, healthier and safer biomedical polymers are discussed. We wish this systemic and comprehensive review on highlighting frontier progress of biomedical polymers could inspire and promote new breakthrough in fundamental research and clinical translation.展开更多
Epigenetic clocks are accurate predictors of human chronological age based on the analysis of DNA methylation(DNAm)at specific CpG sites.However,a systematic comparison between DNA methylation data and other omics dat...Epigenetic clocks are accurate predictors of human chronological age based on the analysis of DNA methylation(DNAm)at specific CpG sites.However,a systematic comparison between DNA methylation data and other omics datasets has not yet been performed.Moreover,available DNAm age predictors are based on datasets with limited ethnic representation.To address these knowledge gaps,we generated and analyzed DNA methylation datasets from two independent Chinese cohorts,revealing age-related DNAm changes.Additionally,a DNA methylation aging clock(iCAS-DNAmAge)and a group of DNAm-based multi-modal clocks for Chinese individuals were developed,with most of them demonstrating strong predictive capabilities for chronological age.The clocks were further employed to predict factors influencing aging rates.The DNAm aging clock,derived from multi-modal aging features(compositeAge-DNAmAge),exhibited a close association with multi-omics changes,lifestyles,and disease status,underscoring its robust potential for precise biological age assessment.Our findings offer novel insights into the regulatory mechanism of age-related DNAm changes and extend the application of the DNAm clock for measuring biological age and aging pace,providing the basis for evaluating aging intervention strategies.展开更多
目的探讨应用儿童急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)临床路径(2010年版)治疗APL患儿的疗效及预后,为APL患儿的治疗提供参考。方法回顾性分析2010年10月1日至2017年9月1日30例初诊为APL患儿的临床资料。根据患儿...目的探讨应用儿童急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)临床路径(2010年版)治疗APL患儿的疗效及预后,为APL患儿的治疗提供参考。方法回顾性分析2010年10月1日至2017年9月1日30例初诊为APL患儿的临床资料。根据患儿入院时外周血WBC、PLT计数分为低危组(WBC≤10×109 L^-1,PLT>40×109 L^-1, n =6)、中危组(WBC≤10×109 L^-1,PLT≤40×109 L^-1, n =12)、高危组(WBC> 10×109 L^-1, n =12),比较3组的发病情况、疗效及预后。结果 30例患儿发病初期多以皮肤黏膜出血、面色苍白和发热为临床表现。弥散性血管内凝血(DIC)发生率为50.0%(15/30),患儿危险度越高,其发生率越高(χ2 =6.460, P =0.034)。诱导治疗中,高危组2例因DIC致颅内出血死亡,另28例患儿诱导化疗后(5周)血液学完全缓解27例(96.4%)。维持治疗中有1例复发。随访中位时间为40个月(0.2~70.0个月),截至2017年9月1日28例患儿存活。高危组5年总生存率、无事件生存率与低、中危组比较,差异无统计学意义(χ2 =3.13、 P =0.209,χ2 =5.34、 P =0.679)。结论 APL的治愈率较高,但高危组早期病死率及复发率仍较高。对DIC及出凝血障碍进行早期干预,制定合理方案减少早期病死率、复发率仍是治疗APL的关键。展开更多
Excited states in the odd-A nucleus 71Ga have been studied via the ^(70)Zn(7Li,a2n)^(71)Ga fusion-evaporation reaction with incident beam energies of 30 and 35 MeV.The level scheme is established up to spin I^(n)=(29/...Excited states in the odd-A nucleus 71Ga have been studied via the ^(70)Zn(7Li,a2n)^(71)Ga fusion-evaporation reaction with incident beam energies of 30 and 35 MeV.The level scheme is established up to spin I^(n)=(29/2^(+))and an excitation energy-6.6 MeV.A previously known sequence built on the 9/2^(+) state is extended as a novel rotational band originating from the v(8^(2)_(9/2)alignment.Furthermore,a negative-parity sequence Is also reported.The observed energy levels of Ga have been interpreted in the framework of the nuclear shell model(SM).展开更多
选取前期实验中从长庆油田措施废液集中处理后残渣中筛选的3株高效石油烃降解菌D1、D2、D5作为研究对象,采用吸附法制备固定化菌剂,制备过程的最佳条件为:秸秆和草炭固定化菌剂的最佳固定化时间均为36 h,木炭的最佳固定化时间为30 h;秸...选取前期实验中从长庆油田措施废液集中处理后残渣中筛选的3株高效石油烃降解菌D1、D2、D5作为研究对象,采用吸附法制备固定化菌剂,制备过程的最佳条件为:秸秆和草炭固定化菌剂的最佳固定化时间均为36 h,木炭的最佳固定化时间为30 h;秸秆固定化菌剂和草炭固定化菌剂的最优载体加入量为3.0g/100 m L菌悬液;木炭固定化菌剂的最优载体加入量为2.0 g/100 m L菌悬液。秸秆固定化的最佳pH为7.0,草炭和木炭固定化的最佳pH为7.5;三种载体固定化菌剂的最佳固定化温度均为35℃。在未灭菌的含油土壤40d的室内原位模拟修复过程中,各固定化菌剂对石油烃的降解率大小依次为:草炭固定化菌剂74.12%>木炭固定化菌剂70.25%>秸秆固定化菌剂60.74%>游离混合菌35.48%>不加菌对照11.98%。在灭菌的含油土壤的修复过程中,几种处理方式对石油烃的降解率大小依次为:木炭固定化菌剂70.75%>草炭固定化菌剂69.90%>秸秆固定化菌剂68.28%>游离混合菌44.30%>不加菌对照2.21%。展开更多
Background:Recent genome-wide association studies have identified an important role of T-cell receptor α(TRA) gene in the development of narcolepsy type 1.However,the role of TRA haplotype polymorphisms in the sympto...Background:Recent genome-wide association studies have identified an important role of T-cell receptor α(TRA) gene in the development of narcolepsy type 1.However,the role of TRA haplotype polymorphisms in the symptomatic diversity of narcolepsy remains unclear.This study aimed to investigate whether TRA polymorphisms can influence the symptomatic diversity of narcolepsy.Methods:Totally,903 patients with narcolepsy type 1 were included in the study.Patients were divided into different groups according to their symptoms.First,13 genotyped single nucleotide polymorphisms in the TRA were assessed for their association with symptoms of narcolepsy.We used the Chi-square test to determine differences in genotype frequencies in patients with narcolepsy.Further,we identified the haplotypes and variations of the TRA and tested their association with the symptoms of narcolepsy using a logistic regression model.Results:According to the results of the logistic regression,TRA haplotypes TG and CT were significantly associated with auditory hallucination,with odds ratios of 1.235 (95% confidence interval [CI],1.012-1.507) and 1.236 (95% CI,1.012-1.511),respectively (P < 0.05).Contusions:The patterns of haplotype in TRA (haplotypes TG and CT) are associated with hypnagogic auditory hallucination in patients with narcolepsy type 1.However,further studies are needed to confirm our results and explore the underlying mechanisms.展开更多
Lifetimes of the 2_(1)^(+),4_(1)^(+),7_(2)^(-),10_(2)^(+),12_(2)^(+),and 14_(1)^(+) states in 138 Nd populated via the 123 Sb(19 F,4n)138 Nd fusion–evaporation reaction were measured with the recoil distance Doppler ...Lifetimes of the 2_(1)^(+),4_(1)^(+),7_(2)^(-),10_(2)^(+),12_(2)^(+),and 14_(1)^(+) states in 138 Nd populated via the 123 Sb(19 F,4n)138 Nd fusion–evaporation reaction were measured with the recoil distance Doppler shift technique in combination with the differential decay curve method.The B(E2;2_(1)^(+)→0_(1)^(+))value fit well with the systematic trend in the Nd isotope chain and Grodzins rule,which proved that 138Nd is a transitional nucleus.展开更多
The bias dependence of radiation-induced narrow-width channel effects（RINCEs） in 65-nm n-type metal-oxidesemiconductor field-effect transistors（NMOSFETs） is investigated. The threshold voltage of the narrow-width6...The bias dependence of radiation-induced narrow-width channel effects（RINCEs） in 65-nm n-type metal-oxidesemiconductor field-effect transistors（NMOSFETs） is investigated. The threshold voltage of the narrow-width65 nm NMOSFET is negatively shifted by total ionizing dose irradiation, due to the RINCE. The experimental results show that the 65 nm narrow-channel NMOSFET has a larger threshold shift when the gate terminal is kept in the ground, which is contrary to the conclusion obtained in the old generation devices. Depending on the three-dimensional simulation, we conclude that electric field distribution alteration caused by shallow trench isolation scaling is responsible for the anomalous RINCE bias dependence in 65 nm technology.展开更多
基金supported by the Chinese Medicine Research Foundation of Jiangxi Provincial Health Department of China,No.2013A040the Science and Technology Program of Jiangxi Provincial Health Department of China,No.20123023the Science and Technology Support Program of Jiangxi Province of China,No.2009BSB11209
文摘Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of baicalin in a neonatal rat model of hypoxic-ischemic encephalopathy.Seven-day-old pups underwent left common carotid artery ligation followed by hypoxia（8% oxygen at 37°C） for 2 hours,before being injected with baicalin（120 mg/kg intraperitoneally） and examined 24 hours later.Baicalin effectively reduced cerebral infarct volume and neuronal loss,inhibited apoptosis,and upregulated the expression of p-Akt and glutamate transporter 1.Intracerebroventricular injection of the phosphoinositide 3-kinase/protein kinase B（PI3 K/Akt） inhibitor LY294002 30 minutes before injury blocked the effect of baicalin on p-Akt and glutamate transporter 1,and weakened the associated neuroprotective effect.Our findings provide the first evidence,to our knowledge that baicalin can protect neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the PI3 K/Akt signaling pathway.
基金supported by the National Natural Science Foundation of China (52073218, 22135005, 51873162, 51933006,51988102, 52122310, 22075050, 51833008, 51733006, 51733001,52122304)Jiangsu Province Science Foundation for Youths(BK20200241)+3 种基金Science and Technology Commission of Shanghai Municipality (20JC1414902, 21511104900)Shanghai Municipal Education Commission (2017-01-07-00-07-E00062)the National Key Research and Development Program (2021YFA1201200) of Chinathe Zhejiang Provincial Key Research and Development Program (2020C01123)。
文摘Biomedical polymers have been extensively developed for promising applications in a lot of biomedical fields, such as therapeutic medicine delivery, disease detection and diagnosis, biosensing, regenerative medicine, and disease treatment. In this review, we summarize the most recent advances in the synthesis and application of biomedical polymers, and discuss the comprehensive understanding of their property-function relationship for corresponding biomedical applications. In particular, a few burgeoning bioactive polymers, such as peptide/biomembrane/microorganism/cell-based biomedical polymers, are also introduced and highlighted as the emerging biomaterials for cancer precision therapy. Furthermore, the foreseeable challenges and outlook of the development of more efficient, healthier and safer biomedical polymers are discussed. We wish this systemic and comprehensive review on highlighting frontier progress of biomedical polymers could inspire and promote new breakthrough in fundamental research and clinical translation.
基金supported by the National Key Research and Development Program of China(2021YFF1201000,2022YFA1103700)the Quzhou Technology Projects(2022K46)+13 种基金the National Natural Science Foundation of China(Grant Nos.32121001,81921006,82125011,92149301,82361148131,82192863)the National Key Research and Development Program of China(2020YFA0804000,2020YFA0112200,the STI2030-Major Projects-2021ZD0202400,2021YFA1101000)the National Natural Science Foundation of China(Grant Nos.92168201,92049304,92049116,82122024,82071588,32000510,8236114813082271600,82322025,82330044,32341001)CAS Project for Young Scientists in Basic Research(YSBR-076,YSBR-012)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB38010400)the Science and Technology Service Network Initiative of Chinese Academy of Sciences(KFJSTS-QYZD-2021-08-001)the Beijing Natural Science Foundation(Z230011,5242024)the Informatization Plan of Chinese Academy of Sciences(CAS-WX2021SF-0301,CAS-WX2022SDC-XK14,CAS-WX2021SF-0101)New Cormerstone Science Foundation through the XPLORER PRIZE(2021-1045)YouthInnovation Promotion Association of CAS(E1CAZW0401,2022083)Excellent Young Talents Program of Capital Medical University(12300927)the Project for Technology Development of Beijing-affliated Medical ResearchInstitutes(11000023T000002036310)ExcellentYoung Talents Training Program for the Construction of Beijing Municipal University Teacher Team(BPHR202203105)Young Elite Scientists Sponsorship Program by CAST(2021QNRC001)Beijing Municipal Public Welfare Development and Reform Pilot Project for Medical Research Institutes(JYY202X-X).
文摘Epigenetic clocks are accurate predictors of human chronological age based on the analysis of DNA methylation(DNAm)at specific CpG sites.However,a systematic comparison between DNA methylation data and other omics datasets has not yet been performed.Moreover,available DNAm age predictors are based on datasets with limited ethnic representation.To address these knowledge gaps,we generated and analyzed DNA methylation datasets from two independent Chinese cohorts,revealing age-related DNAm changes.Additionally,a DNA methylation aging clock(iCAS-DNAmAge)and a group of DNAm-based multi-modal clocks for Chinese individuals were developed,with most of them demonstrating strong predictive capabilities for chronological age.The clocks were further employed to predict factors influencing aging rates.The DNAm aging clock,derived from multi-modal aging features(compositeAge-DNAmAge),exhibited a close association with multi-omics changes,lifestyles,and disease status,underscoring its robust potential for precise biological age assessment.Our findings offer novel insights into the regulatory mechanism of age-related DNAm changes and extend the application of the DNAm clock for measuring biological age and aging pace,providing the basis for evaluating aging intervention strategies.
文摘目的探讨应用儿童急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)临床路径(2010年版)治疗APL患儿的疗效及预后,为APL患儿的治疗提供参考。方法回顾性分析2010年10月1日至2017年9月1日30例初诊为APL患儿的临床资料。根据患儿入院时外周血WBC、PLT计数分为低危组(WBC≤10×109 L^-1,PLT>40×109 L^-1, n =6)、中危组(WBC≤10×109 L^-1,PLT≤40×109 L^-1, n =12)、高危组(WBC> 10×109 L^-1, n =12),比较3组的发病情况、疗效及预后。结果 30例患儿发病初期多以皮肤黏膜出血、面色苍白和发热为临床表现。弥散性血管内凝血(DIC)发生率为50.0%(15/30),患儿危险度越高,其发生率越高(χ2 =6.460, P =0.034)。诱导治疗中,高危组2例因DIC致颅内出血死亡,另28例患儿诱导化疗后(5周)血液学完全缓解27例(96.4%)。维持治疗中有1例复发。随访中位时间为40个月(0.2~70.0个月),截至2017年9月1日28例患儿存活。高危组5年总生存率、无事件生存率与低、中危组比较,差异无统计学意义(χ2 =3.13、 P =0.209,χ2 =5.34、 P =0.679)。结论 APL的治愈率较高,但高危组早期病死率及复发率仍较高。对DIC及出凝血障碍进行早期干预,制定合理方案减少早期病死率、复发率仍是治疗APL的关键。
基金Supported by the National Natural Science Foundation of China(U1932209,11975315,U1867210,11905134)the Leading Innovation Project under Grant(LC192209000701,LC202309000201)+1 种基金the Continuous Basic Scientific Research Project(BJ20002501,WDJC-2019-13)China National Nuclear Corporation(FA18000201)。
文摘Excited states in the odd-A nucleus 71Ga have been studied via the ^(70)Zn(7Li,a2n)^(71)Ga fusion-evaporation reaction with incident beam energies of 30 and 35 MeV.The level scheme is established up to spin I^(n)=(29/2^(+))and an excitation energy-6.6 MeV.A previously known sequence built on the 9/2^(+) state is extended as a novel rotational band originating from the v(8^(2)_(9/2)alignment.Furthermore,a negative-parity sequence Is also reported.The observed energy levels of Ga have been interpreted in the framework of the nuclear shell model(SM).
文摘选取前期实验中从长庆油田措施废液集中处理后残渣中筛选的3株高效石油烃降解菌D1、D2、D5作为研究对象,采用吸附法制备固定化菌剂,制备过程的最佳条件为:秸秆和草炭固定化菌剂的最佳固定化时间均为36 h,木炭的最佳固定化时间为30 h;秸秆固定化菌剂和草炭固定化菌剂的最优载体加入量为3.0g/100 m L菌悬液;木炭固定化菌剂的最优载体加入量为2.0 g/100 m L菌悬液。秸秆固定化的最佳pH为7.0,草炭和木炭固定化的最佳pH为7.5;三种载体固定化菌剂的最佳固定化温度均为35℃。在未灭菌的含油土壤40d的室内原位模拟修复过程中,各固定化菌剂对石油烃的降解率大小依次为:草炭固定化菌剂74.12%>木炭固定化菌剂70.25%>秸秆固定化菌剂60.74%>游离混合菌35.48%>不加菌对照11.98%。在灭菌的含油土壤的修复过程中,几种处理方式对石油烃的降解率大小依次为:木炭固定化菌剂70.75%>草炭固定化菌剂69.90%>秸秆固定化菌剂68.28%>游离混合菌44.30%>不加菌对照2.21%。
文摘Background:Recent genome-wide association studies have identified an important role of T-cell receptor α(TRA) gene in the development of narcolepsy type 1.However,the role of TRA haplotype polymorphisms in the symptomatic diversity of narcolepsy remains unclear.This study aimed to investigate whether TRA polymorphisms can influence the symptomatic diversity of narcolepsy.Methods:Totally,903 patients with narcolepsy type 1 were included in the study.Patients were divided into different groups according to their symptoms.First,13 genotyped single nucleotide polymorphisms in the TRA were assessed for their association with symptoms of narcolepsy.We used the Chi-square test to determine differences in genotype frequencies in patients with narcolepsy.Further,we identified the haplotypes and variations of the TRA and tested their association with the symptoms of narcolepsy using a logistic regression model.Results:According to the results of the logistic regression,TRA haplotypes TG and CT were significantly associated with auditory hallucination,with odds ratios of 1.235 (95% confidence interval [CI],1.012-1.507) and 1.236 (95% CI,1.012-1.511),respectively (P < 0.05).Contusions:The patterns of haplotype in TRA (haplotypes TG and CT) are associated with hypnagogic auditory hallucination in patients with narcolepsy type 1.However,further studies are needed to confirm our results and explore the underlying mechanisms.
基金This work was supported by the National Natural Science Foundation of China(Nos.U1932209,11975315,U1867210,and 11905134)the Leading Innovation Project(Nos.LC192209000701 and LC202309000201)+1 种基金the Continuous Basic Scientific Research Project(Nos.BJ20002501 and WDJC-2019-13)the China National Nuclear Corporation(No.FA18000201).
文摘Lifetimes of the 2_(1)^(+),4_(1)^(+),7_(2)^(-),10_(2)^(+),12_(2)^(+),and 14_(1)^(+) states in 138 Nd populated via the 123 Sb(19 F,4n)138 Nd fusion–evaporation reaction were measured with the recoil distance Doppler shift technique in combination with the differential decay curve method.The B(E2;2_(1)^(+)→0_(1)^(+))value fit well with the systematic trend in the Nd isotope chain and Grodzins rule,which proved that 138Nd is a transitional nucleus.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11605282,11505282 and U1532261the West Light Foundation of the Chinese Academy of Sciences under Grant No 2015-XBQN-B-15
文摘The bias dependence of radiation-induced narrow-width channel effects（RINCEs） in 65-nm n-type metal-oxidesemiconductor field-effect transistors（NMOSFETs） is investigated. The threshold voltage of the narrow-width65 nm NMOSFET is negatively shifted by total ionizing dose irradiation, due to the RINCE. The experimental results show that the 65 nm narrow-channel NMOSFET has a larger threshold shift when the gate terminal is kept in the ground, which is contrary to the conclusion obtained in the old generation devices. Depending on the three-dimensional simulation, we conclude that electric field distribution alteration caused by shallow trench isolation scaling is responsible for the anomalous RINCE bias dependence in 65 nm technology.
