AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province.METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-s...AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province.METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics.RESULTS: Allele frequency (AF) of HLA-DRB1·0901 was significantly higher in esophageal carcinoma patients than that in the normal controls (0.2500 vs0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1·0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles.CONCLUSION: HLA-DRB1·0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1·0901may be susceptible to esophageal carcinoma.展开更多
AIM:To develop a tumor DNA vaccine encoding a fusion protein of murine AFP and CTLA4,and to study its ability to induce specific CTL response and its protective effect against AFP-producing tumor.METHODS:Murine α-fet...AIM:To develop a tumor DNA vaccine encoding a fusion protein of murine AFP and CTLA4,and to study its ability to induce specific CTL response and its protective effect against AFP-producing tumor.METHODS:Murine α-fetoprotein (mAFP) gene was cloned from total RNA of Hepal-6 cells by RT-PCR.A DNA vaccine was constructed by fusion murine α-fetoprotein gene and extramembrane domain of murine CTLA4 gene.The DNA vaccine was identified by restriction enzyme analysis,sequencing and expression.EL-4 (mAFP) was developed by stable transfection of EL-4 cells with pmAFP.The frequency of cells producing IFN-γ in splenocytes harvested from the immunized mice was measured by ELISPOT.Mice immunized with DNA vaccine were inoculated with EL-4(mAFP) cells in back to observe the protective effect of immunization on tumor. On the other hand,blood samples were collected from the immunized mice to check the functions of liver and kidney.RESULTS:1.8kb mAFP cDNA was cloned from total RNA of Hepal-6 cells by RT-PCR.The DNA vaccine encoding a fusion protein of mAFP-CTLA4 was constructed and confirmed by restriction enzyme analysis,sequencing and expression.The expression of mAFP mRNA in EL-4 (mAFP) was confirmed by RT-PCR.The ELISPOT results showedthat the number of IFN-γ-producing cells in pmAFP-CTLA4 group was significantly higher than that in pmAFP,pcDNA3.1 and PBS group.The tumor volume in pmAFP-CTLA4 group was significantly smaller than that in pmAFP,pcDNA3.1 and PBS group, respectively. The hepatic and kidney functions in each group were not altered.CONCLUSION: AFP-CTLA4 DNA vaccine can stimulate potent specific CTL responses and has distinctive antitumor effect on AFP-producing tumor.The vaccine has no impact on the function of mouse liver and kidney.展开更多
LATELY, the ferroelectric random access memories (FRAM) have become one of the most attractive subjects in solid devices due to faster access time as dynamic RAM, the non-volatility as erasable programmable read-only ...LATELY, the ferroelectric random access memories (FRAM) have become one of the most attractive subjects in solid devices due to faster access time as dynamic RAM, the non-volatility as erasable programmable read-only memory, radiation-hardness and lower power展开更多
To analyze the polymorphism of TAP gene and the shared rates of alleles between mothers and their infants in Chinese patients with pre-eclampsia,TAP1 and TAP2 genotyping was performed by the amplification refractory m...To analyze the polymorphism of TAP gene and the shared rates of alleles between mothers and their infants in Chinese patients with pre-eclampsia,TAP1 and TAP2 genotyping was performed by the amplification refractory mutation system-polymerase chain reaction(ARMS-PCR)in 42 patients,106 normal pregnant women,and their neonates.The allelic frequency of TAP and the alleles shared in maternal-fetus were compared and analyzed in the two groups.Our results showed that,with totally eight alleles of TAP1 and TAP2 examined in the samples,no significant difference was found in allelic frequencies between pre-eclampsia group and control group,as well as between mothers and their neonates.Similar finding was obtained in the comparison with shared alleles.In conclusion,our results do not support a role for the polymorphisms of TAP in the etiology of pre-eclampsia. Cellular & Molecular Immunology.2005;2(2):141-144.展开更多
The development of sericultural industry in the Pearl River Delta has contributed greatly to good ecological environment and economic prosperity of the people’s livelihood.Comprehensive utilization of sericulture res...The development of sericultural industry in the Pearl River Delta has contributed greatly to good ecological environment and economic prosperity of the people’s livelihood.Comprehensive utilization of sericulture resources can turn waste into valuable resources and expand the development approaches of sericultural industry.For example,using abundant silkworm excrement to produce chlorophyll is one of the good practices in comprehensive utilization of sericulture resources,which has leading technologies and good economic benefits.In addition to chlorophyll,the products produced from silkworm excrement can be used as the main raw materials of medicine,food,plant growth regulator and cosmetics.For instances,phytol,the important raw material for synthesis of vitamin E and vitamin K,was extracted from silkworm excrement,enabling China to become an important production base of vitamin E and vitamin K.Triacontanol extracted from silkworm excrement is a new crop growth regulator,which can increase the yield of rice and vegetable.Pectin,dietary fiber and carotenoid extracted from silkworm excrement as well can be used as natural raw material in many fields.China is the biggest manufacturer and supplier of silkworm excrement products in the world,which has a development history of about 50 years and generated high export revenue.It is believed that close combination of academic research and industry development is the only way to promote the industrial development of silkworm excrement resources.展开更多
目的评价TRA对HER2阳性BC患者肝毒性和胃肠道不良反应的风险。方法检索PubMed、Cochrane Library、Embase、Web of Science、CNKI及万方数据库,收集TRA治疗HER2阳性BC患者肝毒性和胃肠道不良反应的RCT研究。采用RevMan5.3软件进行Meta...目的评价TRA对HER2阳性BC患者肝毒性和胃肠道不良反应的风险。方法检索PubMed、Cochrane Library、Embase、Web of Science、CNKI及万方数据库,收集TRA治疗HER2阳性BC患者肝毒性和胃肠道不良反应的RCT研究。采用RevMan5.3软件进行Meta分析。结果共纳入23项RCT,结果显示,ALT升高[RR=1.90,95%CI(1.52,2.38),P<0.01]、AST升高[RR=2.18,95%CI(1.79,2.65),P<0.01]发生率显著高于对照组,而恶心、呕吐发生率与对照组相比差异无统计学意义。结论在TRA治疗HER2阳性BC患者后,肝毒性发生率明显升高,胃肠道不良反应发生率无显著差异。因此,在临床上需密切监测肝功能并注意胃肠道不良反应。展开更多
文摘AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province.METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics.RESULTS: Allele frequency (AF) of HLA-DRB1·0901 was significantly higher in esophageal carcinoma patients than that in the normal controls (0.2500 vs0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1·0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles.CONCLUSION: HLA-DRB1·0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1·0901may be susceptible to esophageal carcinoma.
文摘AIM:To develop a tumor DNA vaccine encoding a fusion protein of murine AFP and CTLA4,and to study its ability to induce specific CTL response and its protective effect against AFP-producing tumor.METHODS:Murine α-fetoprotein (mAFP) gene was cloned from total RNA of Hepal-6 cells by RT-PCR.A DNA vaccine was constructed by fusion murine α-fetoprotein gene and extramembrane domain of murine CTLA4 gene.The DNA vaccine was identified by restriction enzyme analysis,sequencing and expression.EL-4 (mAFP) was developed by stable transfection of EL-4 cells with pmAFP.The frequency of cells producing IFN-γ in splenocytes harvested from the immunized mice was measured by ELISPOT.Mice immunized with DNA vaccine were inoculated with EL-4(mAFP) cells in back to observe the protective effect of immunization on tumor. On the other hand,blood samples were collected from the immunized mice to check the functions of liver and kidney.RESULTS:1.8kb mAFP cDNA was cloned from total RNA of Hepal-6 cells by RT-PCR.The DNA vaccine encoding a fusion protein of mAFP-CTLA4 was constructed and confirmed by restriction enzyme analysis,sequencing and expression.The expression of mAFP mRNA in EL-4 (mAFP) was confirmed by RT-PCR.The ELISPOT results showedthat the number of IFN-γ-producing cells in pmAFP-CTLA4 group was significantly higher than that in pmAFP,pcDNA3.1 and PBS group.The tumor volume in pmAFP-CTLA4 group was significantly smaller than that in pmAFP,pcDNA3.1 and PBS group, respectively. The hepatic and kidney functions in each group were not altered.CONCLUSION: AFP-CTLA4 DNA vaccine can stimulate potent specific CTL responses and has distinctive antitumor effect on AFP-producing tumor.The vaccine has no impact on the function of mouse liver and kidney.
文摘LATELY, the ferroelectric random access memories (FRAM) have become one of the most attractive subjects in solid devices due to faster access time as dynamic RAM, the non-volatility as erasable programmable read-only memory, radiation-hardness and lower power
文摘To analyze the polymorphism of TAP gene and the shared rates of alleles between mothers and their infants in Chinese patients with pre-eclampsia,TAP1 and TAP2 genotyping was performed by the amplification refractory mutation system-polymerase chain reaction(ARMS-PCR)in 42 patients,106 normal pregnant women,and their neonates.The allelic frequency of TAP and the alleles shared in maternal-fetus were compared and analyzed in the two groups.Our results showed that,with totally eight alleles of TAP1 and TAP2 examined in the samples,no significant difference was found in allelic frequencies between pre-eclampsia group and control group,as well as between mothers and their neonates.Similar finding was obtained in the comparison with shared alleles.In conclusion,our results do not support a role for the polymorphisms of TAP in the etiology of pre-eclampsia. Cellular & Molecular Immunology.2005;2(2):141-144.
文摘The development of sericultural industry in the Pearl River Delta has contributed greatly to good ecological environment and economic prosperity of the people’s livelihood.Comprehensive utilization of sericulture resources can turn waste into valuable resources and expand the development approaches of sericultural industry.For example,using abundant silkworm excrement to produce chlorophyll is one of the good practices in comprehensive utilization of sericulture resources,which has leading technologies and good economic benefits.In addition to chlorophyll,the products produced from silkworm excrement can be used as the main raw materials of medicine,food,plant growth regulator and cosmetics.For instances,phytol,the important raw material for synthesis of vitamin E and vitamin K,was extracted from silkworm excrement,enabling China to become an important production base of vitamin E and vitamin K.Triacontanol extracted from silkworm excrement is a new crop growth regulator,which can increase the yield of rice and vegetable.Pectin,dietary fiber and carotenoid extracted from silkworm excrement as well can be used as natural raw material in many fields.China is the biggest manufacturer and supplier of silkworm excrement products in the world,which has a development history of about 50 years and generated high export revenue.It is believed that close combination of academic research and industry development is the only way to promote the industrial development of silkworm excrement resources.
文摘目的评价TRA对HER2阳性BC患者肝毒性和胃肠道不良反应的风险。方法检索PubMed、Cochrane Library、Embase、Web of Science、CNKI及万方数据库,收集TRA治疗HER2阳性BC患者肝毒性和胃肠道不良反应的RCT研究。采用RevMan5.3软件进行Meta分析。结果共纳入23项RCT,结果显示,ALT升高[RR=1.90,95%CI(1.52,2.38),P<0.01]、AST升高[RR=2.18,95%CI(1.79,2.65),P<0.01]发生率显著高于对照组,而恶心、呕吐发生率与对照组相比差异无统计学意义。结论在TRA治疗HER2阳性BC患者后,肝毒性发生率明显升高,胃肠道不良反应发生率无显著差异。因此,在临床上需密切监测肝功能并注意胃肠道不良反应。
