Nanocapsules (NC) of antioxidant rich fraction of roasted <span>Moringa </span>leaves were prepared using emulsion coacervation technique with alginate (ALG) and/or chitosan (CTS) as biopolymers. NC were c...Nanocapsules (NC) of antioxidant rich fraction of roasted <span>Moringa </span>leaves were prepared using emulsion coacervation technique with alginate (ALG) and/or chitosan (CTS) as biopolymers. NC were characterized based on particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency (EE) and loading capacity (LC). Substituting CTS with ALG in NC caused a reduction in particle size and PDI, and enhanced EE. Mean particle size dropped from 1209 nm in 1:3 to 413 nm in 3:1 ALG/CTS-NC;PDI decreased from 0.9% to 0.2% and zeta potential from </span></span><span><span><span style="font-family:"">-</span></span></span><span><span><span style="font-family:"">5.4 to </span></span></span><span><span><span style="font-family:"">-</span></span></span><span><span><span style="font-family:"">28.1 mV. </span></span></span><span><span><span style="font-family:"">The </span></span></span><span><span><span style="font-family:"">highest EE (87.6%) and LC (13%) were obtained with ALG-CTS-NC (3:1). ALG-NC were spherical while both CTS and ALG-CTS-NC were ovoid. ALG and ALG-CTS-NC were oil/water emulsions while CTS-NC formed water/oil emulsions. 60% and 70% of bioactives in ALG-CTS-NC (3:1) were released in simulated gastric and intestinal fluids respectively after 400 min. Release of antioxidants from NC is concentration-dependent (First order model) and involves simultaneously diffusion (Higuchi model), swelling (korsmeyer-Peppas model) and erosion (Hixson-Crowell model) mechanisms.展开更多
文摘Nanocapsules (NC) of antioxidant rich fraction of roasted <span>Moringa </span>leaves were prepared using emulsion coacervation technique with alginate (ALG) and/or chitosan (CTS) as biopolymers. NC were characterized based on particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency (EE) and loading capacity (LC). Substituting CTS with ALG in NC caused a reduction in particle size and PDI, and enhanced EE. Mean particle size dropped from 1209 nm in 1:3 to 413 nm in 3:1 ALG/CTS-NC;PDI decreased from 0.9% to 0.2% and zeta potential from </span></span><span><span><span style="font-family:"">-</span></span></span><span><span><span style="font-family:"">5.4 to </span></span></span><span><span><span style="font-family:"">-</span></span></span><span><span><span style="font-family:"">28.1 mV. </span></span></span><span><span><span style="font-family:"">The </span></span></span><span><span><span style="font-family:"">highest EE (87.6%) and LC (13%) were obtained with ALG-CTS-NC (3:1). ALG-NC were spherical while both CTS and ALG-CTS-NC were ovoid. ALG and ALG-CTS-NC were oil/water emulsions while CTS-NC formed water/oil emulsions. 60% and 70% of bioactives in ALG-CTS-NC (3:1) were released in simulated gastric and intestinal fluids respectively after 400 min. Release of antioxidants from NC is concentration-dependent (First order model) and involves simultaneously diffusion (Higuchi model), swelling (korsmeyer-Peppas model) and erosion (Hixson-Crowell model) mechanisms.
