Light-emitting diode(LED)irradiation can affect postharvest vegetable and fruit shelf life and nutritional quality.In this study,11 kinds of glucosinolates(GLS)and 6 types of GLS breakdown products were identified in ...Light-emitting diode(LED)irradiation can affect postharvest vegetable and fruit shelf life and nutritional quality.In this study,11 kinds of glucosinolates(GLS)and 6 types of GLS breakdown products were identified in cabbage leaves.To investigate the effects of LED irradiation on quality and GLS metabolism,postharvest cabbage was irradiated with 25μmol/(m2·s)green LED(GL)light at room temperature for 12 d.The dark condition and natural light were used as controls.GL irradiation inhibited the respiration rate and the accumulation of malondialdehyde,and maintained higher cabbage sensory score,chlorophyll content,and antioxidant enzyme activities,thus extending its postharvest shelf life.Additionally,GL irradiation promoted GLS profile accumulation,including glucoraphanin,glucoiberin,gluconapin,gluconasturtiin,glucobrassicin,and sinigrin,which could have resulted from upregulated expression of the transcription factors MYB28 and MYB51,and the key genes involved in GLS biosynthesis(CYP83A1,CYP83B1,ST5b,and UGT74B1).Furthermore,GLS degradation was promoted by this treatment during the late stage of shelf life,as higher myrosinase activity and relative MYR and ESM expression levels were found in treated cabbage leaves,resulting in higher isothiocyanate(ITC)and indole-3-carbinol contents after day 6.Therefore,GL irradiation was beneficial for cabbage preservation.展开更多
Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed...Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed the senescence of pak choi stored at 20°C.A total of 1121 differentially expressed genes(DEGs)were identified in an MT-treated group in comparison to untreated pak choi.Analysis of DEGs revealed that MT-induced delay in senescence involved reduction in the expression of chlorophyll metabolism genes and regulation of the expression of hormone-related genes and leaf senescence-related transcription factors.MT decreased the activity of chlorophyll metabolism-related enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase,decreased the concentrations of abscisic acid and jasmonic acid,and increased auxin concentration.MT delayed the impact of senescence-related transcription factors BcNAC41 and BcNAC87 on tobacco leaf senescence.This study provides an insight into the molecular mechanisms of MT-induced delay of postharvest senescence in pak choi and confirms its potential as a preservation technique.展开更多
基金the Key and General Programs of Modern Agriculture in Jiangsu Province(No.BE2022368)Jiangsu Agricultural Science and Technology Innovation Fund(No.CX(20)1008),China.
文摘Light-emitting diode(LED)irradiation can affect postharvest vegetable and fruit shelf life and nutritional quality.In this study,11 kinds of glucosinolates(GLS)and 6 types of GLS breakdown products were identified in cabbage leaves.To investigate the effects of LED irradiation on quality and GLS metabolism,postharvest cabbage was irradiated with 25μmol/(m2·s)green LED(GL)light at room temperature for 12 d.The dark condition and natural light were used as controls.GL irradiation inhibited the respiration rate and the accumulation of malondialdehyde,and maintained higher cabbage sensory score,chlorophyll content,and antioxidant enzyme activities,thus extending its postharvest shelf life.Additionally,GL irradiation promoted GLS profile accumulation,including glucoraphanin,glucoiberin,gluconapin,gluconasturtiin,glucobrassicin,and sinigrin,which could have resulted from upregulated expression of the transcription factors MYB28 and MYB51,and the key genes involved in GLS biosynthesis(CYP83A1,CYP83B1,ST5b,and UGT74B1).Furthermore,GLS degradation was promoted by this treatment during the late stage of shelf life,as higher myrosinase activity and relative MYR and ESM expression levels were found in treated cabbage leaves,resulting in higher isothiocyanate(ITC)and indole-3-carbinol contents after day 6.Therefore,GL irradiation was beneficial for cabbage preservation.
基金the National Natural Science Foundation of China(No.32001451)the Key and General Programs of Modern Agriculture in Jiangsu Province(No.BE2022368),China.
文摘Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed the senescence of pak choi stored at 20°C.A total of 1121 differentially expressed genes(DEGs)were identified in an MT-treated group in comparison to untreated pak choi.Analysis of DEGs revealed that MT-induced delay in senescence involved reduction in the expression of chlorophyll metabolism genes and regulation of the expression of hormone-related genes and leaf senescence-related transcription factors.MT decreased the activity of chlorophyll metabolism-related enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase,decreased the concentrations of abscisic acid and jasmonic acid,and increased auxin concentration.MT delayed the impact of senescence-related transcription factors BcNAC41 and BcNAC87 on tobacco leaf senescence.This study provides an insight into the molecular mechanisms of MT-induced delay of postharvest senescence in pak choi and confirms its potential as a preservation technique.
