Objective:To explore the expression of Nrf2/ARE pathway in hindbrain tissue after the traumatic brain injury（TBI） and its anli-oxidative stress effect in the secondary nerve injury.Methods: The mice with Nrf2 gene k...Objective:To explore the expression of Nrf2/ARE pathway in hindbrain tissue after the traumatic brain injury（TBI） and its anli-oxidative stress effect in the secondary nerve injury.Methods: The mice with Nrf2 gene knockout were used for the establishment of brain injury model.The experimental animals were divided into four groups:(Nrf2<sup>+/+</sup>) sham-operation group,(Nrf2<sup>+/+</sup>) brain injury group,（Nrf2T） sham-operation group and(Nrf2<sup>-/-</sup>) brain injury group.The specimen 24 h after cerebral trauma was selected.Then RT-PCR method was adopted to detect the expression of Nrf2 mRNA in brain;Western blotting method was adopted to detect the levels of Nrf2,HO-1 and NQO1 proteins in brain;EUSA method was adopted to detect the oxidative stress indicators: protein carbonyls,4-hydroxy-2-nonenal（4-HNE） and 8-hydroxy-2’-deoxyguanosine（8-OHdG）. ResuHs:The Nrf2 mRNA and protein of Nrf2<sup>-/-</sup> mice were not expressed,and the diflerence of the relative amount of Nrf2 mRNA between Nrf2<sup>+/+</sup> TBI group and Nrf2<sup>+/+</sup> sham-operation group was not statistically significant（P】0.05）;the level of Nrf2 protein in Nrf2<sup>+/+</sup> TBI group increased significantly compared with the Nrf2<sup>+/+</sup> sham-operation group（P【0.01）;in the sham-operation groups,the levels of HO-1 and NQO1 proteins of Nrf2<sup>-/-</sup> mice decreased obviously compared with the Nrf2<sup>+/+</sup> mice（P【0.01）;after brain injury,the levels of HO-1 and NQO1 proteins of Nrf2<sup>+/+</sup> mice increased obviously compared with the corresponding sham-operation group（P【0.01）;the levels of HO-1 and NQOl proteins of Nrf2<sup>-/-</sup> mice in TBI group had no obvious change compared with the corresponding sham-operation group（P】0.05）;there was only a little amount of expression of protein carbonyls,4-HNE and 8-OHdG proteins in brain tissues in the Nrf2<sup>-/+</sup> and Nrf2<sup>-/-</sup> shamoperation groups,and the difference was not statistically significant（P&展开更多
文摘Objective:To explore the expression of Nrf2/ARE pathway in hindbrain tissue after the traumatic brain injury（TBI） and its anli-oxidative stress effect in the secondary nerve injury.Methods: The mice with Nrf2 gene knockout were used for the establishment of brain injury model.The experimental animals were divided into four groups:(Nrf2<sup>+/+</sup>) sham-operation group,(Nrf2<sup>+/+</sup>) brain injury group,（Nrf2T） sham-operation group and(Nrf2<sup>-/-</sup>) brain injury group.The specimen 24 h after cerebral trauma was selected.Then RT-PCR method was adopted to detect the expression of Nrf2 mRNA in brain;Western blotting method was adopted to detect the levels of Nrf2,HO-1 and NQO1 proteins in brain;EUSA method was adopted to detect the oxidative stress indicators: protein carbonyls,4-hydroxy-2-nonenal（4-HNE） and 8-hydroxy-2’-deoxyguanosine（8-OHdG）. ResuHs:The Nrf2 mRNA and protein of Nrf2<sup>-/-</sup> mice were not expressed,and the diflerence of the relative amount of Nrf2 mRNA between Nrf2<sup>+/+</sup> TBI group and Nrf2<sup>+/+</sup> sham-operation group was not statistically significant（P】0.05）;the level of Nrf2 protein in Nrf2<sup>+/+</sup> TBI group increased significantly compared with the Nrf2<sup>+/+</sup> sham-operation group（P【0.01）;in the sham-operation groups,the levels of HO-1 and NQO1 proteins of Nrf2<sup>-/-</sup> mice decreased obviously compared with the Nrf2<sup>+/+</sup> mice（P【0.01）;after brain injury,the levels of HO-1 and NQO1 proteins of Nrf2<sup>+/+</sup> mice increased obviously compared with the corresponding sham-operation group（P【0.01）;the levels of HO-1 and NQOl proteins of Nrf2<sup>-/-</sup> mice in TBI group had no obvious change compared with the corresponding sham-operation group（P】0.05）;there was only a little amount of expression of protein carbonyls,4-HNE and 8-OHdG proteins in brain tissues in the Nrf2<sup>-/+</sup> and Nrf2<sup>-/-</sup> shamoperation groups,and the difference was not statistically significant（P&
