Aims: Rotavirus-associated enteritis is a major problem in livestock, notably in young piglets and calves, and is also a zoonosis. It is also associated with diarrhoea mainly in children less than five years of age. I...Aims: Rotavirus-associated enteritis is a major problem in livestock, notably in young piglets and calves, and is also a zoonosis. It is also associated with diarrhoea mainly in children less than five years of age. In Tanzania however, no study has addressed Rotavirus in livestock species. Following our previous report on Rotavirus infection in children within urban and peri-urban Arusha, we sought to understand the disease situation in livestock in the same area. Study Design: Place and Duration of Study: In this study, we investigated the prevalence of Rotavirus in pigs of suckling, weaning and post weaning/grazing/fattening age categories in Lemara, Moshono and Sokoni I areas of Arusha peri-urban. Methodology: Molecular detection of Rotavirus in stool samples was done using conventional PCR with primers targeting Group A Rotavirus (GARV). Using a standardized questionnaire, we sought to find out risk factors associated with positive cases of Rotavirus including age, sex, location, diarrhoea status, recent diarrhoea case in the farm, breed, type of grazing system and type of feeding of individual pigs. Results: Out of a total of 110 pigs sampled (fecal samples), 41.8% were positive for Rotavirus. Chi Square’s (χ<sup>2</sup>) Fisher’s Exact Test was used to relate PCR test results with various possible risk factors. Recent diarrhoea case in the farm was significantly (p < 0.05) associated with Rotavirus infection in pigs indicating the possible role of cross-infection within farm and also the environmental resistance and persistence of the virus in the farm. Conclusions: This was the first study to report on Rotavirus infection in pigs in Tanzania. The information obtained should form the platform for further studies to address the molecular epidemiology and relatedness of Rotavirus from human and porcine positive cases.展开更多
Aims: Anaemia in children aged 6 - 59 months is an important public health problem associated with increasing hospital costs, lengths of hospital stay, and development of complications later in life. Due to the signif...Aims: Anaemia in children aged 6 - 59 months is an important public health problem associated with increasing hospital costs, lengths of hospital stay, and development of complications later in life. Due to the significant caregiver roles of mothers, the level of mothers’ knowledge, beliefs and practices are important in addressing anaemia in children. This study investigated knowledge, attitudes, beliefs, and practices of select mothers on anaemia and linked these with known factors for anaemia. Methodology: The case study was conducted at Mkuranga District Hospital, Paediatric Ward between December 2014 and April 2015. A cross-sectional design was used to recruit a convenience sample of 40 mothers whose children had a confirmed diagnosis of anaemia (through routine laboratory testing). Results: Within the sample, the majority of children were male (52.5%);aged 6 - 39 months (87.5%);and had a diagnosis of severe anaemia (75.0%) according to the World Health Organization’s definition. Over one third (35%) of mothers reported a prior history of anemia in their other children, and the majority (55%) had heard about anaemia prior to their child’s hospitalization. Maternal anaemia was reported by 67.5% of mothers. Mothers reported that maternal anemia (17.5%) and feeding practices (32.5%) are known contributing factors to anaemia in children. Mothers reported that anemia could be prevented (55.0%) and cured by herbal preparations (47.5%). In addition, some mothers indicated that anaemia was caused by witchcraft (22.5%) and eating lemons (2.5%). Conclusion: Severe anaemia was high among the studied population which aligned with their hospitalization status. Findings suggested potential gaps in control and management of anemia in children possibly related to low awareness or incorrect knowledge of the relationship between maternal and child anaemia. The findings also highlighted important cultural beliefs related to anaemia. There is an imperative for culturally and socially appropriate knowledge translation and 展开更多
文摘Aims: Rotavirus-associated enteritis is a major problem in livestock, notably in young piglets and calves, and is also a zoonosis. It is also associated with diarrhoea mainly in children less than five years of age. In Tanzania however, no study has addressed Rotavirus in livestock species. Following our previous report on Rotavirus infection in children within urban and peri-urban Arusha, we sought to understand the disease situation in livestock in the same area. Study Design: Place and Duration of Study: In this study, we investigated the prevalence of Rotavirus in pigs of suckling, weaning and post weaning/grazing/fattening age categories in Lemara, Moshono and Sokoni I areas of Arusha peri-urban. Methodology: Molecular detection of Rotavirus in stool samples was done using conventional PCR with primers targeting Group A Rotavirus (GARV). Using a standardized questionnaire, we sought to find out risk factors associated with positive cases of Rotavirus including age, sex, location, diarrhoea status, recent diarrhoea case in the farm, breed, type of grazing system and type of feeding of individual pigs. Results: Out of a total of 110 pigs sampled (fecal samples), 41.8% were positive for Rotavirus. Chi Square’s (χ<sup>2</sup>) Fisher’s Exact Test was used to relate PCR test results with various possible risk factors. Recent diarrhoea case in the farm was significantly (p < 0.05) associated with Rotavirus infection in pigs indicating the possible role of cross-infection within farm and also the environmental resistance and persistence of the virus in the farm. Conclusions: This was the first study to report on Rotavirus infection in pigs in Tanzania. The information obtained should form the platform for further studies to address the molecular epidemiology and relatedness of Rotavirus from human and porcine positive cases.
文摘Aims: Anaemia in children aged 6 - 59 months is an important public health problem associated with increasing hospital costs, lengths of hospital stay, and development of complications later in life. Due to the significant caregiver roles of mothers, the level of mothers’ knowledge, beliefs and practices are important in addressing anaemia in children. This study investigated knowledge, attitudes, beliefs, and practices of select mothers on anaemia and linked these with known factors for anaemia. Methodology: The case study was conducted at Mkuranga District Hospital, Paediatric Ward between December 2014 and April 2015. A cross-sectional design was used to recruit a convenience sample of 40 mothers whose children had a confirmed diagnosis of anaemia (through routine laboratory testing). Results: Within the sample, the majority of children were male (52.5%);aged 6 - 39 months (87.5%);and had a diagnosis of severe anaemia (75.0%) according to the World Health Organization’s definition. Over one third (35%) of mothers reported a prior history of anemia in their other children, and the majority (55%) had heard about anaemia prior to their child’s hospitalization. Maternal anaemia was reported by 67.5% of mothers. Mothers reported that maternal anemia (17.5%) and feeding practices (32.5%) are known contributing factors to anaemia in children. Mothers reported that anemia could be prevented (55.0%) and cured by herbal preparations (47.5%). In addition, some mothers indicated that anaemia was caused by witchcraft (22.5%) and eating lemons (2.5%). Conclusion: Severe anaemia was high among the studied population which aligned with their hospitalization status. Findings suggested potential gaps in control and management of anemia in children possibly related to low awareness or incorrect knowledge of the relationship between maternal and child anaemia. The findings also highlighted important cultural beliefs related to anaemia. There is an imperative for culturally and socially appropriate knowledge translation and
