Background: Necrotizing fasciitis is a soft tissue infection that occurs predominantly in adults. Although rare, only 70 cases have been reported in neonates. This entity, most commonly caused by multiple organisms is...Background: Necrotizing fasciitis is a soft tissue infection that occurs predominantly in adults. Although rare, only 70 cases have been reported in neonates. This entity, most commonly caused by multiple organisms is often fatal, with 50% mortality. The combination of a low incidence and high mortality in this subgroup strengthens the need for early diagnosis, prompt recognition and immediate surgical treatment in order to improve survival. Aim: The aim of this case report is to bring awareness of a clinical condition that relies mostly on physical examination and where early diagnosis and immediate surgical treatment are needed in order to improve survival. Case presentation: We report the case of an 8-day-old boy presenting neonatal necrotizing fasciitis after circumcision. Patient presented with lower back erythema and areas of fluctuation. He was started on antibiotic therapy and 24 hours after admission, he was taken to the operating room for multiple fasciotomies, debridement and cleansing of affected areas. Findings were consistent with necrotizing fasciitis. After surgical intervention, local care was given until complete healing was achieved, approximately one month after admission. Conclusion: The surgeon must be attentive for the possible occurrence of this lethal infection that may develop post operatively after clean or clean-contaminated procedures. Preparation for treating these patients immediately with adequate fluid resuscitation, appropriate antibiotics and aggressive surgical debridement of non-viable tissue will provide the maximal potential for recovery. These prompt measures will offer the best chance for survival and eventually decrease the 50% mortality rate associated to the neonatal population.展开更多
Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with human...Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.展开更多
Cervical uterine cancer represents the fourth most common malignant neoplasm worldwide in the female sex in terms of incidence,<span><span><span style="color:black;"> </span></span...Cervical uterine cancer represents the fourth most common malignant neoplasm worldwide in the female sex in terms of incidence,<span><span><span style="color:black;"> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">principally from epithelial origen. The high expression of EGFR in this tumor leads to the search for therapeutic alternatives. An Expanded Access Clinical Program was carried out in parallel groups, randomized, multicenter and prospective study, to evaluate the survival of patients with advanced cervical carcinoma, without therapeutic alternative, who would be treated with the therapeutic vaccine CIMAvax-EGF<sup></sup></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="white-space:nowrap;"><sup>®</sup></span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">, the humanized mAb nimotuzumab </span><span style="font-family:Verdana;">or</span><span style="font-family:Verdana;"> the combination of both products, which targeted EGF and EGFR respectively. The patients were included between 2008 and 2010 with </span><span style="font-family:Verdana;">a more</span><span style="font-family:Verdana;"> than five years follow-up. The results show that the serious adverse events related to the experimental treatments were 0.9</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">%</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">;1.1</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">%</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> and 2.6% and a median ITT survival of 9.1, 23.5, and 16.3 months fo展开更多
文摘Background: Necrotizing fasciitis is a soft tissue infection that occurs predominantly in adults. Although rare, only 70 cases have been reported in neonates. This entity, most commonly caused by multiple organisms is often fatal, with 50% mortality. The combination of a low incidence and high mortality in this subgroup strengthens the need for early diagnosis, prompt recognition and immediate surgical treatment in order to improve survival. Aim: The aim of this case report is to bring awareness of a clinical condition that relies mostly on physical examination and where early diagnosis and immediate surgical treatment are needed in order to improve survival. Case presentation: We report the case of an 8-day-old boy presenting neonatal necrotizing fasciitis after circumcision. Patient presented with lower back erythema and areas of fluctuation. He was started on antibiotic therapy and 24 hours after admission, he was taken to the operating room for multiple fasciotomies, debridement and cleansing of affected areas. Findings were consistent with necrotizing fasciitis. After surgical intervention, local care was given until complete healing was achieved, approximately one month after admission. Conclusion: The surgeon must be attentive for the possible occurrence of this lethal infection that may develop post operatively after clean or clean-contaminated procedures. Preparation for treating these patients immediately with adequate fluid resuscitation, appropriate antibiotics and aggressive surgical debridement of non-viable tissue will provide the maximal potential for recovery. These prompt measures will offer the best chance for survival and eventually decrease the 50% mortality rate associated to the neonatal population.
文摘Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.
文摘Cervical uterine cancer represents the fourth most common malignant neoplasm worldwide in the female sex in terms of incidence,<span><span><span style="color:black;"> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">principally from epithelial origen. The high expression of EGFR in this tumor leads to the search for therapeutic alternatives. An Expanded Access Clinical Program was carried out in parallel groups, randomized, multicenter and prospective study, to evaluate the survival of patients with advanced cervical carcinoma, without therapeutic alternative, who would be treated with the therapeutic vaccine CIMAvax-EGF<sup></sup></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="white-space:nowrap;"><sup>®</sup></span></span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">, the humanized mAb nimotuzumab </span><span style="font-family:Verdana;">or</span><span style="font-family:Verdana;"> the combination of both products, which targeted EGF and EGFR respectively. The patients were included between 2008 and 2010 with </span><span style="font-family:Verdana;">a more</span><span style="font-family:Verdana;"> than five years follow-up. The results show that the serious adverse events related to the experimental treatments were 0.9</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">%</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">;1.1</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">%</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> and 2.6% and a median ITT survival of 9.1, 23.5, and 16.3 months fo
