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海南省奶源和养殖环境主要肠杆菌科细菌的分布及基因分型
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作者 郝若晨 唐敏嘉 +5 位作者 刘光亮 张艳 muhammad shoaib 尚若锋 曹宗喜 蒲万霞 《畜牧兽医学报》 CAS CSCD 北大核心 2023年第12期5184-5197,共14页
本研究从2021年海南省两个奶牛场采集的52份样本中分离肠杆菌科细菌,并对分离菌株进行了毒力基因、质粒型和生物被膜形成能力检测及肠杆菌科细菌基因间重复序列分子分型(ERIC-PCR)研究,以期明确牛奶及奶牛场环境中肠杆菌科细菌分布流行... 本研究从2021年海南省两个奶牛场采集的52份样本中分离肠杆菌科细菌,并对分离菌株进行了毒力基因、质粒型和生物被膜形成能力检测及肠杆菌科细菌基因间重复序列分子分型(ERIC-PCR)研究,以期明确牛奶及奶牛场环境中肠杆菌科细菌分布流行状况及其生物学特性。首先,采用16S rDNA PCR和ERIC-PCR对分离的肠杆菌科细菌进行鉴定和去重,结果显示52份样本中分离到肠杆菌科细菌49株,其中大肠埃希菌(Escherichia coli)32株,肺炎克雷伯菌(Klebsiella pneumoniae)13株,阴沟肠杆菌(Enterobacter cloacae)3株,产气肠杆菌(Enterobacter aerogenes)1株;其次,采用PCR方法进行了分离菌株的毒力基因检测,结果显示,ompA的检出率最高;之后,采用CARATTOLI创建的基于PCR的质粒复制子分型,结果显示K质粒的检出率最高;采用刚果红琼脂法对分离菌株进行了生物被膜形成能力定性检测,发现49株肠杆菌科细菌中有37株指示有生物被膜形成能力;最后,通过ERIC-PCR分型进行了肠杆菌科细菌的亲缘关系和遗传多样性分析,发现49株肠杆菌科菌株可划分为17型(Ⅰ~ⅩⅦ),Ⅵ型为优势型(均为Escherichia coli),共20株。整体研究表明肠杆菌科细菌在奶源环境中广泛存在、生物特性多样,推测具有一定的致病力和耐药性,引起人类和动物疾病风险较高,提示养殖、运输中的卫生和规范操作至关重要。 展开更多
关键词 肠杆菌科细菌 分子分型 质粒型 生物被膜
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Aggregate Suitability Assessment of Wargal Limestone for Pavement Construction in Pakistan 被引量:2
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作者 Javid Hussain Jiaming Zhang +4 位作者 Fitriani Fitria muhammad shoaib Hadi Hussain Ali Asghar Sadam Hussain 《Open Journal of Civil Engineering》 2022年第1期56-74,共19页
This paper presents the evaluation of the potential aggregate source for pavement construction in Pakistan. Recently the demand for construction materials has been increased significantly due to the establishment of t... This paper presents the evaluation of the potential aggregate source for pavement construction in Pakistan. Recently the demand for construction materials has been increased significantly due to the establishment of the China-Pakistan Economic Corridor (CPEC) Projects. Therefore, it is essential to look for new resources of construction materials along with the CPEC routes in consideration of this increasing demand. In this context, a Physical and Mechanical characterization investigation is carried out on the Permian Wargal Limestone from Zaluch Nala, Salt Range to explore their potential to utilize as construction materials. The studied samples have tolerable values for all standard engineering parameters, proposed by various national and international agencies such as AASHTO, ASTM, BS, and NHA. Furthermore, as a performance indicator of aggregate overall quality, the evaluated mechanical qualities were integrated into a single characteristic, Toughness Index (TI). The TI values also suggested that the Permian Wargal limestone aggregates meet international quality standards for pavement construction. On the basis of geotechnical testing and Toughness Index (TI), the late Permian Wargal limestone, Zaluch Nala, Salt Range, is strongly recommended as a potential aggregate resource for mega projects such as the China-Pakistan Economic Corridor (CPEC) and other construction projects. 展开更多
关键词 CPEC Potential Aggregates Geotechnical Properties Toughness Index (TI) Wargal Limestone
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PCR Based Detection and Phylogenetic Analysis of Fowl Adenovirus Strains Isolated from 2019 Epidemic from Punjab and Sindh, Pakistan 被引量:1
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作者 Nadeem Sharif muhammad Danish Mehmood +5 位作者 Syed Zeeshan Haider Naqvi Huma Anwar ul-Haq Syed shoaib Ahmed muhammad Usman Ghani muhammad shoaib Mubashir Hussain 《American Journal of Molecular Biology》 2020年第3期246-258,共13页
Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in deva... Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in devastating damage, causing huge economic losses to its farmers. In present study, molecular typing is performed on basis of partially conserved hexon gene sequences, using a unique set of primers having common reverse oligo for simultaneous detection of FAdV1, FAdV-4 and FAdV-11. A total of 14 fowl adeno virus strains were isolated from 100 suspected adeno virus liver samples, collected from different districts in Pakistan, between 2018 and 2019. FASTA’s sequence alignment and phylogenetic analysis revealed that out of the 14, one isolate which belonged to group A showed 27% similarity with FAdV-1, while three isolates showed 99%, 95% & 45% similarity to FAdV-4 (Group C). Whereas, ten isolates showed more than 99% similarity to FAdV-11 (Group D). The serotypes FAdV1, FAdV-4 and FAdV-11 are prevailing in the breeder and broilers. These results hold great importance in rapid, reliable and simultaneous detection of the three FAdV serotypes. Therefore, fowl adeno virus vaccine production for commercial poultry shall be according to the prevalent field serotypes. 展开更多
关键词 Hydro-Pericardium Hepatitis (HPH) Hexon Gene Polymerase Chain Reaction Phylogenetic Analysis Sanger’s Sequence
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Measurement of Acceleration Due to Gravity Using Arduino and Ultrasonic Sensor
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作者 muhammad shoaib Asad muhammad Iqbal muhammad Arham Imran 《Journal of Sensor Technology》 2021年第4期55-63,共9页
In this study, a simple Arduino-based experiment was designed to examine the acceleration of the object during free fall and to calculate the value of “g” (acceleration due to gravity). Experimental data on the free... In this study, a simple Arduino-based experiment was designed to examine the acceleration of the object during free fall and to calculate the value of “g” (acceleration due to gravity). Experimental data on the free fall of a plastic box through the air was gathered with the help of an ultrasonic distance sensor (HC-SR04). Readings were taken at different intervals during the fall to obtain distance time curves. Acceleration during the free fall was then determined by applying the standard kinematic equations. The shape of the distance-time graphs obtained from the experimental setup was in good agreement with the predicted graphs and the calculated values of g lie within the expected range. After repeated experiments, value of gravitational acceleration was found to be 9.805 m/s2. Hardware and software prepared for the experiment are sufficient to examine movement of ordinary objects during free fall, therefore the experiment can be easily settled in a laboratory for the purpose of learning and teaching. 展开更多
关键词 ARDUINO Acceleration Due to Gravity Basic Physics Experiment
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和阻抗继电器的改进形式及其动作性能分析 被引量:5
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作者 吕冉 李慧 +8 位作者 刘鹏 林湘宁 童宁 李正天 黄景光 邓科 Owolabi Sunday Adio muhammad shoaib Khalid 韩雄辉 《中国电机工程学报》 EI CSCD 北大核心 2018年第7期2001-2011,共11页
现有高压输电线路保护对于单相高阻接地故障灵敏度不足,长期以来一直是继电保护领域的一个遗留问题。该文根据线路双端测量阻抗之和在正常运行及单相故障场景下的差异性,针对原有和阻抗继电器不能动态适应线路电容电流变化的缺陷,提出... 现有高压输电线路保护对于单相高阻接地故障灵敏度不足,长期以来一直是继电保护领域的一个遗留问题。该文根据线路双端测量阻抗之和在正常运行及单相故障场景下的差异性,针对原有和阻抗继电器不能动态适应线路电容电流变化的缺陷,提出一种改进和阻抗继电器,并设计能够保障其区内全线高阻接地速动能力的自适应浮动门槛。仿真结果证明所提出的原理实现简单、无需双端信息同步,能够快速响应区内任意故障点单相高阻故障,具备适应系统非全相运行能力,在绝大多数情况下,耐受过渡电阻能力均能达到300Ω。通过与现有主保护配合使用,可显著提升高压输电线路保护对各种系统运行工况下单相高阻接地故障的响应能力。 展开更多
关键词 输电线路 高阻接地 改进和阻抗继电器 过渡电阻 自适应门槛
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乙酰唑胺对低氧应激下SD大鼠肺泡膜通透性的影响 被引量:1
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作者 杜忠蕾 muhammad shoaib Tahir +6 位作者 高源 马倩倩 朱沁芳 刘杰 刘美恒 张欢 张伟 《解放军医学院学报》 CAS 北大核心 2022年第3期340-346,共7页
背景急进高原容易产生高原肺水肿(high altitude pulmonary edema,HAPE)。乙酰唑胺作为国际通用预防急性高原病的首选药,在低氧应激过程中的调节作用尚不清楚,尤其对肺泡膜通透性的影响还不明确。目的研究乙酰唑胺对低氧应激下SD大鼠肺... 背景急进高原容易产生高原肺水肿(high altitude pulmonary edema,HAPE)。乙酰唑胺作为国际通用预防急性高原病的首选药,在低氧应激过程中的调节作用尚不清楚,尤其对肺泡膜通透性的影响还不明确。目的研究乙酰唑胺对低氧应激下SD大鼠肺泡膜通透性的影响。方法将雄性SD大鼠随机分为4组(每组12只):对照组(CN组,西宁海拔2260 m)、低氧组(H7K组,模拟海拔7000 m)、乙酰唑胺组(NAZ组,西宁海拔2260 m)、低氧+乙酰唑胺组(HAZ组,模拟海拔7000 m)。NAZ、HAZ组给予1%乙酰唑胺(50 mg/kg),CN、H7K组给予等容量的0.9%氯化钠注射液(45 mg/kg),灌胃(Bid,共5 d)。灌胃3 d后将H7K、HAZ组大鼠置于低压氧舱中2 d(模拟海拔7000 m,大气压305 mmHg,PO_(2)63.7 mmHg;1 mmHg=0.133 kPa)。测定大鼠动脉pH、二氧化碳分压(PaCO_(2))、氧分压(PaO_(2))、碳酸氢盐(HCO_(3)^(-))等;测定右肺组织湿重/干重(W/D);ELISA测定支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及血清白细胞介素6(interleukin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)含量;qRT-PCR检测左肺组织水通道蛋白1(aquaporin 1,AQP1)、AQP5的mRNA表达量;免疫组化检测肺组织中AQP1和AQP5的表达水平。观察各组肺组织形态结构变化。结果与CN组比较,NAZ组pH、PaCO_(2)下降;H7K组动脉血pH值下降,PaO_(2)降低(P<0.05),右肺组织的W/D升高;BALF及血清中IL-6、TNF-α含量增高;左肺组织的AQP1、AQP5 mRNA及蛋白表达量均升高(P<0.05)。H7K组的肺组织切片HE染色见肺泡上皮细胞肿胀明显,肺泡间隔增宽伴渗出。与H7K组比较,HAZ组动脉血PaO_(2)升高(P<0.05);W/D比值降低(P<0.05);BALF中IL-6含量降低,血清中IL-6、TNF-α含量降低(P<0.05)。左肺组织的AQP1、AQP5 mRNA及蛋白表达量减少(P<0.05)。HAZ组的肺组织切片HE染色可见肺泡上皮细胞偶有肿胀,肺泡间隔略增宽,肺水肿程度减轻。结论乙酰唑胺预处理能减少IL-6、TNF-α的释放和AQP1、AQP5的 展开更多
关键词 低氧 应激 肺泡膜 通透性 乙酰唑胺 水通道蛋白
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Development and Aging Behaviour of Solvent-Based Polychloroprene Rubber Nano-Adhesive Using Multiwall Carbon Nanotubes
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作者 muhammad Awais muhammad Shahid +3 位作者 Mohsin Saleem Fariz Aneeq muhammad shoaib Butt Malik Adeel Umer 《Journal of Materials Science and Chemical Engineering》 2020年第9期31-44,共14页
Polychloroprene (PC) based contact adhesives are widely used in various applications;however, there is a possibility to improve the properties of PC adhesive. Modifications of polymers can enhance the properties of th... Polychloroprene (PC) based contact adhesives are widely used in various applications;however, there is a possibility to improve the properties of PC adhesive. Modifications of polymers can enhance the properties of the material, e.g. increase in thermal stability, compatibility, rigidity, physical response, flexibility and improve the polymer process ability. In the current study, improved formulation of solvent-based adhesive was developed, and the properties were further enhanced by the addition of nano-reinforcement of multiwall carbon nanotubes (MWCNTs). The addition of nano-reinforcement was optimized to obtain improvement in the bond strength and also to enhance its resistance at a high temperature (~100<span style="white-space:nowrap;">&#176;</span>C). This paper discusses the uniform dispersion of MWCNTs during the synthesis of polychloroprene solvent-based adhesive, thereby improving its structural properties. Incorporation of MWCNTs-solvent-based adhesives resulted in a 20% - 35% improvement in 180<span style="white-space:nowrap;">&#176;</span> peel strength determined on flexible substrates such as canvas, leather. The reinforced based adhesive also exhibited improved thermal stability and weather resistance compared with unreinforced adhesive. The MWCNTs- solvent-based contact adhesives is a potential candidate in an industrially relevant branch of adhesives commonly used in structural applications, e.g., footwear, plastic, leather, automobile, construction industries, etc. 展开更多
关键词 NANOCOMPOSITE POLYCHLOROPRENE ADHESIVE Peel Strength Carbon Nanotubes
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Power Efficient Scheduled-Based Medium Access Control Protocol over Wireless Sensor Networks
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作者 muhammad shoaib Khan Abrar Alajlan Marwah Almasri 《Wireless Sensor Network》 2016年第2期13-23,共11页
The flexible use of sensors has made them an attractive device to be deployed in almost every field of life such as health, military and home. Recent advancement in electronics and wireless communications has witnesse... The flexible use of sensors has made them an attractive device to be deployed in almost every field of life such as health, military and home. Recent advancement in electronics and wireless communications has witnessed the development of low cost-sensor devices. While wireless sensor networks (WSNs) are flexible to use and less costly, they need to be more energy-efficient as they are operated by the battery. Mostly they are deployed in harsh environments where it is very difficult to change the batteries frequently. Several medium access control (MAC) algorithms have been developed for the energy-efficient acquisition of the wireless channel, however, none of them are satisfactory. In this paper, we proposed a medium access control algorithm called MAC-PE. MAC-PE is based on the concept of prioritized frames where prioritized frames are transmitted urgently. In addition, it uses scheduled-based MAC instead of accessing channel randomly. We found MAC-PE (Power-Efficient MAC) was efficient in terms of power consumption without sacrificing on the performance using NS-2. 展开更多
关键词 PE-MAC S-MAC G-MAC T-MAC Power Consumption MOBILITY
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