Little is known about how chronic inflammation contributes to the progression of hepatoceUular carcinoma （HCC）, especially the initiation of cancer. To uncover the critical transition from chronic inflammation to HC...Little is known about how chronic inflammation contributes to the progression of hepatoceUular carcinoma （HCC）, especially the initiation of cancer. To uncover the critical transition from chronic inflammation to HCC and the molecular mechanisms at a network level, we analyzed the time-series proteomic data of woodchuck hepatitis virus/c.myc mice and age-matched wt-C57BL/6 mice using our dynamical network biomarker （DNB） model. DNB analysis indicated that the 5th month after birth of transgenic mice was the critical period of cancer initiation, just before the critical transition, which is consistent with clinical symptoms. Meanwhile, the DNB-associated network showed a drastic inversion of protein expression and coexpression levels before and after the critical transition. Two members of DNB, PLA2G6 and CYP2C44, along with their associated differentially expressed proteins, were found to induce dysfunction of arachidonic acid metabolism, further activate inflammatory responses through inflammatory mediator regulation of transient receptor potential channels, and finally lead to impairments of liver detoxification and malignant transition to cancer. As a c-Myc target, PLA2G6 positively correlated with c-Myc in expression, showing a trend from decreasing to increasing during carcinogenesis, with the minimal point at the critical transition or tipping point. Such trend of homologous PLA2G6 and c-Myc was also observed during human hepatocarcinogenesis, with the minimal point at high-grade dysplastic nodules （a stage just before the carcinogenesis）. Our study implies that PLA2G6 might function as an oncogene like famous c-Myc during hepatocar- cinogenesis, while downregulation of PLA2G6 and c-Myc could be a warning signal indicating imminent carcinogenesis.展开更多
The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphe...The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the inhibition activity for peroxidation of linoleic acid, and the reduction power. The methanol-extracts of moso bamboo culms and madake bamboo leaves presented stronger antioxidant activity compared with DPPH scavenging activity. Methanol-extract of moso bamboo culms was further fractionated by different solvents and n-butanol soluble fraction exhibited the most significant activity in the DPPH scavenging assay. The fractionation of n-butanol soluble extract was isolated by silica gel column with gradient mixture solvent of chloroform and methanol. The isolated fractions were directed by the antioxidant activity measured by scavenging the stable DPPH free radical. It was observed that most of the eluted fractions showed the antioxidative activity. Fractions acquired from elution with the mixture solvent of chloroform and methanol (10:1–5:1) showed stronger antioxidant activity than the other fractions.展开更多
Sortilin-related receptor 1(SORL1)is a critical gene associated with late-onset Alzheimer’s disease.SORL1 contributes to the development and progression of this neurodegenerative condition by affecting the transport ...Sortilin-related receptor 1(SORL1)is a critical gene associated with late-onset Alzheimer’s disease.SORL1 contributes to the development and progression of this neurodegenerative condition by affecting the transport and metabolism of intracellularβ-amyloid precursor protein.To better understand the underlying mechanisms of SORL1 in the pathogenesis of late-onset Alzheimer s disease,in this study,we established a mouse model of SorI1 gene knockout using cluste red regularly inters paced short palindro mic repeats-associated protein 9 technology.We found that Sorl1-knocko ut mice displayed deficits in learning and memory.Furthermore,the expression of brain-derived neurotrophic factor was significantly downregulated in the hippocampus and co rtex,and amyloidβ-protein deposits were observed in the brains of 5orl1-knockout mice.In vitro,hippocampal neuronal cell synapses from homozygous Sorl1-knockout mice were impaired.The expression of synaptic proteins,including Drebrin and NR2B,was significantly reduced,and also their colocalization.Additionally,by knocking out the Sorl1 gene in N2a cells,we found that expression of the N-methyl-D-aspartate receptor,NR2B,and cyclic adenosine monophosphate-response element binding protein was also inhibited.These findings suggest that SORL1 participates in the pathogenesis of late-onset Alzheimer s disease by regulating the N-methyl-D-aspartate receptor NR2B/cyclic adenosine monophosphate-response element binding protein signaling axis.展开更多
Mutations in the microrchidia CW-type zinc finger protein 2(MORC2)gene are the causative agent of Charcot-Marie-Tooth disease type 2Z(CMT2Z),and the hotspot mutation p.S87L is associated with a more seve re spinal mus...Mutations in the microrchidia CW-type zinc finger protein 2(MORC2)gene are the causative agent of Charcot-Marie-Tooth disease type 2Z(CMT2Z),and the hotspot mutation p.S87L is associated with a more seve re spinal muscular atrophy-like clinical phenotype.The aims of this study were to determine the mechanism of the severe phenotype caused by the MORC2 p.S87L mutation and to explore potential treatment strategies.Epithelial cells were isolated from urine samples from a spinal muscular atrophy(SMA)-like patient[MORC2 p.S87L),a CMT2Z patient[MORC2 p.Q400R),and a healthy control and induced to generate pluripotent stem cells,which were then differentiated into motor neuron precursor cells.Next-generation RNA sequencing followed by KEGG pathway enrichment analysis revealed that differentially expressed genes involved in the PI3K/Akt and MAP K/ERK signaling pathways were enriched in the p.S87L SMA-like patient group and were significantly downregulated in induced pluripotent stem cells.Reduced proliferation was observed in the induced pluripotent stem cells and motor neuron precursor cells derived from the p.S87L SMA-like patient group compared with the CMT2Z patient group and the healthy control.G0/G1 phase cell cycle arrest was observed in induced pluripotent stem cells derived from the p.S87L SMA-like patient.MORC2 p.S87Lspecific antisense oligonucleotides(p.S87L-ASO-targeting)showed significant efficacy in improving cell prolife ration and activating the PI3K/Akt and MAP K/ERK pathways in induced pluripotent stem cells.Howeve r,p.S87L-ASO-ta rgeting did not rescue prolife ration of motor neuron precursor cells.These findings suggest that downregulation of the PI3K/Akt and MAP K/ERK signaling pathways leading to reduced cell proliferation and G0/G1 phase cell cycle arrest in induced pluripotent stem cells might be the underlying mechanism of the severe p.S87L SMA-like phenotype.p.S87L-ASO-targeting treatment can alleviate disordered cell proliferation in the early stage of pluripotent stem cell induction.展开更多
Powering a moon base,especially keeping it warm during the long lunar night,is a big challenge.This paper introduces a photovoltaic/thermal(PV/T)system incorporating regolith thermal storage to solve the challenge of ...Powering a moon base,especially keeping it warm during the long lunar night,is a big challenge.This paper introduces a photovoltaic/thermal(PV/T)system incorporating regolith thermal storage to solve the challenge of power and heat provision for the lunar base simultaneously.The vacuum of space around the moon helps this system by reducing heat loss.During the moon's daytime,the system not only generates electricity but also captures heat.This stored heat in the regolith is then used at night,reducing the amount of equipment we need to send from Earth.The spectrally selective PV/T panels are designed to absorb a wide range of sunlight(0.3–2.5μm)while minimizing heat loss in the infrared range(3–30μm).Simulation results of the hybrid solar energy system indicate the average value of the overall efficiency is 45.9%,which relatively elevates 56.1%compared to the PV system.The launch mass of the proposed PV/T system is only 8.4%of a traditional photovoltaic-lithium battery system with the same amount of energy storage.And the total specific energy of the proposed system is 7.3 kWh kg^(-1),while that of the photovoltaic-lithium battery system is about 0.3 kWh kg^(-1).In summary,this study proposes an alternative combined heat and electricity supply system for the lunar base,which can greatly reduce the launch mass and free up load for other scientific research equipment.展开更多
Our lab has constructed a new nonviral vec-tor—hrDNA targeting vector(pHrneo). pHrneo is a human derived vector that can target gene into human ribosomal DNA(hrDNA) locus. In this study, we inserted expression casset...Our lab has constructed a new nonviral vec-tor—hrDNA targeting vector(pHrneo). pHrneo is a human derived vector that can target gene into human ribosomal DNA(hrDNA) locus. In this study, we inserted expression cassette of reconstructive hF Ⅷ (hFVIII-BDDAK39) to pHrneo to construct targeting vector: pHrneo-BDDAK39. Through electroporation of pHrneo-BDDAK39 into HT1080 cells, we identified the homologous recombinants by PCR and Southen blotting, and tested the expression of hFVIII- BDDAK39 in the hrDNA locus. The hFⅧ-BDDAK39 was successfully targeted into hrDNA locus of HT-1080 by pHrneo-BDDAK39, and the efficiency of site-specific inte-gration was 2.0×10?5. hFⅧ-BDDAK39 in hrDNA locus of HT-1080 is found to be able to express efficiently (32±5 ng·106 cells?1·24 h?1). Targeting vector pHrneo-BDDAK39 can find use in gene therapy for hemophilia.展开更多
Segregation and inclusion precipitation are the common behaviours of steel solidification, which are resulted from the redistribution and diffusion of the solute elements at the solid-liquid interface. The effect of t...Segregation and inclusion precipitation are the common behaviours of steel solidification, which are resulted from the redistribution and diffusion of the solute elements at the solid-liquid interface. The effect of the phase transition of high-sulfur free-cutting steel is quantified in the present work for the solute partition coefficient(ki), inclusion precipitation, and microsegregation by establishing a coupling model of microsegregation and inclusion precipitation, wherein the quantified dependencies of ki in terms of temperature, phase and carbon(C) content were applied. Results showed that the solidification temperature range and phase transition of high-sulfur steel that under different solidification paths and C contents were quite different, leading to differences in ki and eventually in microsegregation. kC,kP, and kS were mainly affected by phase composition and kSi was primarily by temperature, while kMn depended on both phase composition and temperature during solidification. Increasing the C content within the interval 0.07-0.48 wt%, the ‘proportion of the δ phase maintained temperature region during solidification’(Pδ), kave Pand kave S(kiave, the average value of the ki across the whole stages of solidification)decreased monotonically, whereas kave Cincreased linearly. The peritectic reaction impacted on the phase composition and ki, leading to the change in microsegregation. Such effect of the peritectic reaction was more significant at the last stage of solidification. When the Pδ was between 75% and 100%(corresponding to 0.07-0.16 wt% C), the solidification path resulted in a greater effect on the microsegregation of solutes C, P, and S because of the peritectic reaction. The microsegregation of solutes Mn and S were comprehensively influenced by kMn, kS and Mn S precipitation as well. The studies would help reveal the solute redistribution at the solid-liquid interface, and improve the segregation of high-sulfur steel by controlling the solidification and precipitation in practice.展开更多
Disparities in the substrate affinity and tolerance threshold for ammonia have been believed to play a key role in driving niche differentiation between ammonia-oxidizing archaea (AOA) and bacteria (AOB);however, rece...Disparities in the substrate affinity and tolerance threshold for ammonia have been believed to play a key role in driving niche differentiation between ammonia-oxidizing archaea (AOA) and bacteria (AOB);however, recent surveys argue that direct competition between AOA and AOB is also important in this phenomenon. Accordingly, it is reasonable to predict that diverse AOA lineages would grow in ammonium (NH_(4)^(+))-rich alkaline arable soils if AOB growth is suppressed. To test this hypothesis, a microcosm study was established using three different types of alkaline arable soils, in which a high NH_(4)^(+) concentration (200 μg N g^(-1) dry soil) was maintained by routinely replenishing urea and the activities of AOB were selectively inhibited by 1-octyne or 3,4-dimethylpyrazole phosphate (DMPP). Compared with amendment with urea alone, 1-octyne partially retarded AOB growth, while DMPP completely inhibited AOB. Both inhibitors accelerated the growth of AOA, with significantly higher ratios of abundance of AOA to AOB observed with DMPP amendment across soils. Nonmetric multidimensional scaling analysis (NMDS) indicated that different treatments significantly altered the community structures of both AOA and AOB and AOA OTUs enriched by high-NH_(4)^(+) amendment were taxonomically constrained across the soils tested and closely related to Nitrososphaera viennensis EN76 and N. garnensis. Given that these representative strains have been demonstrated to be sensitive to high ammonia concentrations, our results suggest that it is the competitiveness for ammonia, rather than disparities in substrate affinity and tolerance threshold for ammonia, that drives niche differentiation between these phylotypes and AOB in NH_(4)^(+)-rich alkaline soils.展开更多
Landslides induced by the 2008 Wenchuan earthquake in the Longmenshan area were relatively well instrumented, which makes it possible to investigate the landslides using ground motion records. Firstly, this paper anal...Landslides induced by the 2008 Wenchuan earthquake in the Longmenshan area were relatively well instrumented, which makes it possible to investigate the landslides using ground motion records. Firstly, this paper analyzes the data from Wenchuan earthquake on both regional and local site scale. The analyses show that the Newmark accumulative displacement calculated from the ground motion recorded in a particular geological hazard zone corresponds to the hazard intensity in that zone; the larger the displacement, the more serious the geologic hazard. The calculated result also shows that the displacement is related to the Arias intensity, which represents the total energy released during the earthquake at the observation site. Secondly, this paper constructs an evaluation model of Newmark displacement calculated with Arias intensities to estimate the subsequent slope failure resulting from the earthquake. The calculated results based on the model fit well with the distribution of actual landslides, suggesting that this method is useful for hazard evaluation. Therefore, this type of model can be used for estimating regional-scale distribution of earthquake-induced landslides and their associated hazards immediately after an earthquake.展开更多
Compression molding of glass optics is gradually becoming a viable fabrication technique for high precision optical lenses. However, refractive index variation was observed in compression molded glass lenses, which wo...Compression molding of glass optics is gradually becoming a viable fabrication technique for high precision optical lenses. However, refractive index variation was observed in compression molded glass lenses, which would contribute to image quality degradation. In this research, annealing experiments were applied to control the refractive index variation in molded glass lenses. The refractive index variations pre and post annealing experiment in molded lenses were measured by an experiment setup based on Mach-Zehnder interferometer. The experimental results showed that the refractive index variation can be controlled providing that a proper cooling process is applied during cooling.展开更多
With the rapid development of big data and artificial intelligence(AI),the cloud platform architecture system is constantly developing,optimizing,and improving.As such,new applications,like deep computing and high-per...With the rapid development of big data and artificial intelligence(AI),the cloud platform architecture system is constantly developing,optimizing,and improving.As such,new applications,like deep computing and high-performance computing,require enhanced computing power.To meet this requirement,a non-uniform memory access(NUMA)configuration method is proposed for the cloud computing system according to the affinity,adaptability,and availability of the NUMA architecture processor platform.The proposed method is verified based on the test environment of a domestic central processing unit(CPU).展开更多
Objective:This study investigates the efficacy of analyzing fetal heart rate(FHR)signals based on Artificial Intelligence to obtain a baseline calculation and identify accelerations/decelerations in the FHR through el...Objective:This study investigates the efficacy of analyzing fetal heart rate(FHR)signals based on Artificial Intelligence to obtain a baseline calculation and identify accelerations/decelerations in the FHR through electronic fetal monitoring during labor.Methods:A total of 43,888 cardiotocograph(CTG)records of female patients in labor from January 2012 to December 2020 were collected from the NanFang Hospital of Southern Medical University.After filtering the data,2341 FHR records were used for the study.The ObVue fetal monitoring system,manufactured by Lian-Med Technology Co.Ltd.,was used to monitor the FHR signals for these pregnant women from the beginning of the first stage of labor to the end of delivery.Two obstetric experts together annotated the FHR signals in the system to determine the baseline as well as accelerations/decelerations of the FHR.Our cardiotocograph network(CTGNet)as well as traditional methods were then used to automatically analyze the baseline and acceleration/deceleration of the FHR signals.The results of calculations were compared with the annotations provided by the obstetric experts,and ten-fold cross-validation was applied to evaluate them.The root-mean-square difference(RMSD)between the baselines,acceleration F-measure(Acc.F-measure),deceleration F-measure(Dec.F-measure),coefficient of synthetic inconsistency(SI)and the morphological analysis discordance index(MADI)were used as evaluation metrics.The data were analyzed by using a pairedt-test.Results:The proposed CTGNet was superior to the best traditional method,proposed by Mantel,in terms of the RMSD.BL(1.7935±0.8099vs.2.0293±0.9267,t=-3.55,P=0.004),Acc.F-measure(86.8562±10.9422vs.72.2367±14.2096,t=12.43,P<0.001),Dec.F-measure(72.1038±33.2592vs.58.5040±38.0276,t=4.10,P<0.001),SI(34.8277±20.9595vs.54.8049±25.0265,t=-9.39,P<0.001),and MADI(3.1741±1.9901vs.3.7289±2.7253,t=-2.74,P=0.012).The proposed CTGNet thus had significant advantages over the best traditional method on all evaluation metrics.Conclusion:The proposed A展开更多
Dear Editor,Nowadays,two autologous CAR19-T drugs,Tisagenlecleucel(Kymriah^(TM))and axicabtagene ciloleucel(Yescarta^(TM)),have been approved for the treatment of B cell leukemia and lymphoma and achieved unprecedente...Dear Editor,Nowadays,two autologous CAR19-T drugs,Tisagenlecleucel(Kymriah^(TM))and axicabtagene ciloleucel(Yescarta^(TM)),have been approved for the treatment of B cell leukemia and lymphoma and achieved unprecedented successes.However,about 10-20%of B-ALL patients receiving CAR19-T drugs didn't achieve complete remission(CR),while 30~50%of patients achieved CR would relapse mainly within 1 year.Moreover,the high CR rate of CAR19-T therapy for B-ALL can't be recaptured in other B-NHLs,'such as Burkitt's lymphoma(BL).Therefore,there is an urgent need to improve the therapeutic efficacy of CAR19-T cells.3.展开更多
文摘Little is known about how chronic inflammation contributes to the progression of hepatoceUular carcinoma （HCC）, especially the initiation of cancer. To uncover the critical transition from chronic inflammation to HCC and the molecular mechanisms at a network level, we analyzed the time-series proteomic data of woodchuck hepatitis virus/c.myc mice and age-matched wt-C57BL/6 mice using our dynamical network biomarker （DNB） model. DNB analysis indicated that the 5th month after birth of transgenic mice was the critical period of cancer initiation, just before the critical transition, which is consistent with clinical symptoms. Meanwhile, the DNB-associated network showed a drastic inversion of protein expression and coexpression levels before and after the critical transition. Two members of DNB, PLA2G6 and CYP2C44, along with their associated differentially expressed proteins, were found to induce dysfunction of arachidonic acid metabolism, further activate inflammatory responses through inflammatory mediator regulation of transient receptor potential channels, and finally lead to impairments of liver detoxification and malignant transition to cancer. As a c-Myc target, PLA2G6 positively correlated with c-Myc in expression, showing a trend from decreasing to increasing during carcinogenesis, with the minimal point at the critical transition or tipping point. Such trend of homologous PLA2G6 and c-Myc was also observed during human hepatocarcinogenesis, with the minimal point at high-grade dysplastic nodules （a stage just before the carcinogenesis）. Our study implies that PLA2G6 might function as an oncogene like famous c-Myc during hepatocar- cinogenesis, while downregulation of PLA2G6 and c-Myc could be a warning signal indicating imminent carcinogenesis.
文摘The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the inhibition activity for peroxidation of linoleic acid, and the reduction power. The methanol-extracts of moso bamboo culms and madake bamboo leaves presented stronger antioxidant activity compared with DPPH scavenging activity. Methanol-extract of moso bamboo culms was further fractionated by different solvents and n-butanol soluble fraction exhibited the most significant activity in the DPPH scavenging assay. The fractionation of n-butanol soluble extract was isolated by silica gel column with gradient mixture solvent of chloroform and methanol. The isolated fractions were directed by the antioxidant activity measured by scavenging the stable DPPH free radical. It was observed that most of the eluted fractions showed the antioxidative activity. Fractions acquired from elution with the mixture solvent of chloroform and methanol (10:1–5:1) showed stronger antioxidant activity than the other fractions.
基金supported by the Community Development Office of Hunan Provincial Science and Technology DepartmentChina,Nos.2020SK53613(to DH),21JJ31006(to DH)the Fundamental Research Funds of Central South University,Nos.CX20220375(to TX),2023zzts215(to MZ)。
文摘Sortilin-related receptor 1(SORL1)is a critical gene associated with late-onset Alzheimer’s disease.SORL1 contributes to the development and progression of this neurodegenerative condition by affecting the transport and metabolism of intracellularβ-amyloid precursor protein.To better understand the underlying mechanisms of SORL1 in the pathogenesis of late-onset Alzheimer s disease,in this study,we established a mouse model of SorI1 gene knockout using cluste red regularly inters paced short palindro mic repeats-associated protein 9 technology.We found that Sorl1-knocko ut mice displayed deficits in learning and memory.Furthermore,the expression of brain-derived neurotrophic factor was significantly downregulated in the hippocampus and co rtex,and amyloidβ-protein deposits were observed in the brains of 5orl1-knockout mice.In vitro,hippocampal neuronal cell synapses from homozygous Sorl1-knockout mice were impaired.The expression of synaptic proteins,including Drebrin and NR2B,was significantly reduced,and also their colocalization.Additionally,by knocking out the Sorl1 gene in N2a cells,we found that expression of the N-methyl-D-aspartate receptor,NR2B,and cyclic adenosine monophosphate-response element binding protein was also inhibited.These findings suggest that SORL1 participates in the pathogenesis of late-onset Alzheimer s disease by regulating the N-methyl-D-aspartate receptor NR2B/cyclic adenosine monophosphate-response element binding protein signaling axis.
基金supported by the National Natural Science Foundation of China,Nos.82171172(to RZ)and 81771366(to RZ)Fundamental Research Funds for the Central Universities of Central South University,Nos.2021zzts1095(to SZ)and 2022zzts0832(to HY)。
文摘Mutations in the microrchidia CW-type zinc finger protein 2(MORC2)gene are the causative agent of Charcot-Marie-Tooth disease type 2Z(CMT2Z),and the hotspot mutation p.S87L is associated with a more seve re spinal muscular atrophy-like clinical phenotype.The aims of this study were to determine the mechanism of the severe phenotype caused by the MORC2 p.S87L mutation and to explore potential treatment strategies.Epithelial cells were isolated from urine samples from a spinal muscular atrophy(SMA)-like patient[MORC2 p.S87L),a CMT2Z patient[MORC2 p.Q400R),and a healthy control and induced to generate pluripotent stem cells,which were then differentiated into motor neuron precursor cells.Next-generation RNA sequencing followed by KEGG pathway enrichment analysis revealed that differentially expressed genes involved in the PI3K/Akt and MAP K/ERK signaling pathways were enriched in the p.S87L SMA-like patient group and were significantly downregulated in induced pluripotent stem cells.Reduced proliferation was observed in the induced pluripotent stem cells and motor neuron precursor cells derived from the p.S87L SMA-like patient group compared with the CMT2Z patient group and the healthy control.G0/G1 phase cell cycle arrest was observed in induced pluripotent stem cells derived from the p.S87L SMA-like patient.MORC2 p.S87Lspecific antisense oligonucleotides(p.S87L-ASO-targeting)showed significant efficacy in improving cell prolife ration and activating the PI3K/Akt and MAP K/ERK pathways in induced pluripotent stem cells.Howeve r,p.S87L-ASO-ta rgeting did not rescue prolife ration of motor neuron precursor cells.These findings suggest that downregulation of the PI3K/Akt and MAP K/ERK signaling pathways leading to reduced cell proliferation and G0/G1 phase cell cycle arrest in induced pluripotent stem cells might be the underlying mechanism of the severe p.S87L SMA-like phenotype.p.S87L-ASO-targeting treatment can alleviate disordered cell proliferation in the early stage of pluripotent stem cell induction.
基金supported by the National Natural Science Foundation of China(Grant Nos.52106276 and 52130601)Fundamental Research Funds for the Central Universities(Grant No.WK2090000038)the Joint research center for multi-energy complementation and conversion of USTC。
文摘Powering a moon base,especially keeping it warm during the long lunar night,is a big challenge.This paper introduces a photovoltaic/thermal(PV/T)system incorporating regolith thermal storage to solve the challenge of power and heat provision for the lunar base simultaneously.The vacuum of space around the moon helps this system by reducing heat loss.During the moon's daytime,the system not only generates electricity but also captures heat.This stored heat in the regolith is then used at night,reducing the amount of equipment we need to send from Earth.The spectrally selective PV/T panels are designed to absorb a wide range of sunlight(0.3–2.5μm)while minimizing heat loss in the infrared range(3–30μm).Simulation results of the hybrid solar energy system indicate the average value of the overall efficiency is 45.9%,which relatively elevates 56.1%compared to the PV system.The launch mass of the proposed PV/T system is only 8.4%of a traditional photovoltaic-lithium battery system with the same amount of energy storage.And the total specific energy of the proposed system is 7.3 kWh kg^(-1),while that of the photovoltaic-lithium battery system is about 0.3 kWh kg^(-1).In summary,this study proposes an alternative combined heat and electricity supply system for the lunar base,which can greatly reduce the launch mass and free up load for other scientific research equipment.
基金This work was supported by Chinese 973 Projects (Grant No. 2004CB518800)； 863 Projects (Grant Nos. 2002BA7IIA07-08, 2002BA7l1A07-03 & 2002AA227011)；the National Natural Science Foundation of China (Grant No. 31830200) ； Life Science Research Foundation of Hunan Province.
文摘Our lab has constructed a new nonviral vec-tor—hrDNA targeting vector(pHrneo). pHrneo is a human derived vector that can target gene into human ribosomal DNA(hrDNA) locus. In this study, we inserted expression cassette of reconstructive hF Ⅷ (hFVIII-BDDAK39) to pHrneo to construct targeting vector: pHrneo-BDDAK39. Through electroporation of pHrneo-BDDAK39 into HT1080 cells, we identified the homologous recombinants by PCR and Southen blotting, and tested the expression of hFVIII- BDDAK39 in the hrDNA locus. The hFⅧ-BDDAK39 was successfully targeted into hrDNA locus of HT-1080 by pHrneo-BDDAK39, and the efficiency of site-specific inte-gration was 2.0×10?5. hFⅧ-BDDAK39 in hrDNA locus of HT-1080 is found to be able to express efficiently (32±5 ng·106 cells?1·24 h?1). Targeting vector pHrneo-BDDAK39 can find use in gene therapy for hemophilia.
基金financially supported by the National Natural Science Foundation of China (Nos. 51504048, 51611130062, 51874059 and 51874060)the Natural Science Foundation of Chongqing (No. cstc2018jcyjAX0647)the Fundamental Research Funds for the Central Universities of China (No. cqu2018CDHB1B05)
文摘Segregation and inclusion precipitation are the common behaviours of steel solidification, which are resulted from the redistribution and diffusion of the solute elements at the solid-liquid interface. The effect of the phase transition of high-sulfur free-cutting steel is quantified in the present work for the solute partition coefficient(ki), inclusion precipitation, and microsegregation by establishing a coupling model of microsegregation and inclusion precipitation, wherein the quantified dependencies of ki in terms of temperature, phase and carbon(C) content were applied. Results showed that the solidification temperature range and phase transition of high-sulfur steel that under different solidification paths and C contents were quite different, leading to differences in ki and eventually in microsegregation. kC,kP, and kS were mainly affected by phase composition and kSi was primarily by temperature, while kMn depended on both phase composition and temperature during solidification. Increasing the C content within the interval 0.07-0.48 wt%, the ‘proportion of the δ phase maintained temperature region during solidification’(Pδ), kave Pand kave S(kiave, the average value of the ki across the whole stages of solidification)decreased monotonically, whereas kave Cincreased linearly. The peritectic reaction impacted on the phase composition and ki, leading to the change in microsegregation. Such effect of the peritectic reaction was more significant at the last stage of solidification. When the Pδ was between 75% and 100%(corresponding to 0.07-0.16 wt% C), the solidification path resulted in a greater effect on the microsegregation of solutes C, P, and S because of the peritectic reaction. The microsegregation of solutes Mn and S were comprehensively influenced by kMn, kS and Mn S precipitation as well. The studies would help reveal the solute redistribution at the solid-liquid interface, and improve the segregation of high-sulfur steel by controlling the solidification and precipitation in practice.
基金supported by the National Key Research and Development Program of China(Nos.2017YFD0200707 and 2017YFD0200102)the Fundamental Research Funds for the Central Universities of China(No.2019FZJD007)for Yongchao LIANGthe National Natural Science Foundation of China(No.31800418)for Chang YIN.
文摘Disparities in the substrate affinity and tolerance threshold for ammonia have been believed to play a key role in driving niche differentiation between ammonia-oxidizing archaea (AOA) and bacteria (AOB);however, recent surveys argue that direct competition between AOA and AOB is also important in this phenomenon. Accordingly, it is reasonable to predict that diverse AOA lineages would grow in ammonium (NH_(4)^(+))-rich alkaline arable soils if AOB growth is suppressed. To test this hypothesis, a microcosm study was established using three different types of alkaline arable soils, in which a high NH_(4)^(+) concentration (200 μg N g^(-1) dry soil) was maintained by routinely replenishing urea and the activities of AOB were selectively inhibited by 1-octyne or 3,4-dimethylpyrazole phosphate (DMPP). Compared with amendment with urea alone, 1-octyne partially retarded AOB growth, while DMPP completely inhibited AOB. Both inhibitors accelerated the growth of AOA, with significantly higher ratios of abundance of AOA to AOB observed with DMPP amendment across soils. Nonmetric multidimensional scaling analysis (NMDS) indicated that different treatments significantly altered the community structures of both AOA and AOB and AOA OTUs enriched by high-NH_(4)^(+) amendment were taxonomically constrained across the soils tested and closely related to Nitrososphaera viennensis EN76 and N. garnensis. Given that these representative strains have been demonstrated to be sensitive to high ammonia concentrations, our results suggest that it is the competitiveness for ammonia, rather than disparities in substrate affinity and tolerance threshold for ammonia, that drives niche differentiation between these phylotypes and AOB in NH_(4)^(+)-rich alkaline soils.
基金supported by Institute of Crustal Dynamics,China Earthquake Administration(No.ZDJ2010-28)the National Natural Science Foundation of China(No.40872209)
文摘Landslides induced by the 2008 Wenchuan earthquake in the Longmenshan area were relatively well instrumented, which makes it possible to investigate the landslides using ground motion records. Firstly, this paper analyzes the data from Wenchuan earthquake on both regional and local site scale. The analyses show that the Newmark accumulative displacement calculated from the ground motion recorded in a particular geological hazard zone corresponds to the hazard intensity in that zone; the larger the displacement, the more serious the geologic hazard. The calculated result also shows that the displacement is related to the Arias intensity, which represents the total energy released during the earthquake at the observation site. Secondly, this paper constructs an evaluation model of Newmark displacement calculated with Arias intensities to estimate the subsequent slope failure resulting from the earthquake. The calculated results based on the model fit well with the distribution of actual landslides, suggesting that this method is useful for hazard evaluation. Therefore, this type of model can be used for estimating regional-scale distribution of earthquake-induced landslides and their associated hazards immediately after an earthquake.
文摘Compression molding of glass optics is gradually becoming a viable fabrication technique for high precision optical lenses. However, refractive index variation was observed in compression molded glass lenses, which would contribute to image quality degradation. In this research, annealing experiments were applied to control the refractive index variation in molded glass lenses. The refractive index variations pre and post annealing experiment in molded lenses were measured by an experiment setup based on Mach-Zehnder interferometer. The experimental results showed that the refractive index variation can be controlled providing that a proper cooling process is applied during cooling.
基金the National Key Research and Development Program of China(No.2017YFC0212100)National High-tech R&D Program of China(No.2015AA015308).
文摘With the rapid development of big data and artificial intelligence(AI),the cloud platform architecture system is constantly developing,optimizing,and improving.As such,new applications,like deep computing and high-performance computing,require enhanced computing power.To meet this requirement,a non-uniform memory access(NUMA)configuration method is proposed for the cloud computing system according to the affinity,adaptability,and availability of the NUMA architecture processor platform.The proposed method is verified based on the test environment of a domestic central processing unit(CPU).
基金supported by National Key Research and Development Project(2019YFC0121907 and 2019YFC0120100)Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization(2021B1212040007)。
文摘Objective:This study investigates the efficacy of analyzing fetal heart rate(FHR)signals based on Artificial Intelligence to obtain a baseline calculation and identify accelerations/decelerations in the FHR through electronic fetal monitoring during labor.Methods:A total of 43,888 cardiotocograph(CTG)records of female patients in labor from January 2012 to December 2020 were collected from the NanFang Hospital of Southern Medical University.After filtering the data,2341 FHR records were used for the study.The ObVue fetal monitoring system,manufactured by Lian-Med Technology Co.Ltd.,was used to monitor the FHR signals for these pregnant women from the beginning of the first stage of labor to the end of delivery.Two obstetric experts together annotated the FHR signals in the system to determine the baseline as well as accelerations/decelerations of the FHR.Our cardiotocograph network(CTGNet)as well as traditional methods were then used to automatically analyze the baseline and acceleration/deceleration of the FHR signals.The results of calculations were compared with the annotations provided by the obstetric experts,and ten-fold cross-validation was applied to evaluate them.The root-mean-square difference(RMSD)between the baselines,acceleration F-measure(Acc.F-measure),deceleration F-measure(Dec.F-measure),coefficient of synthetic inconsistency(SI)and the morphological analysis discordance index(MADI)were used as evaluation metrics.The data were analyzed by using a pairedt-test.Results:The proposed CTGNet was superior to the best traditional method,proposed by Mantel,in terms of the RMSD.BL(1.7935±0.8099vs.2.0293±0.9267,t=-3.55,P=0.004),Acc.F-measure(86.8562±10.9422vs.72.2367±14.2096,t=12.43,P<0.001),Dec.F-measure(72.1038±33.2592vs.58.5040±38.0276,t=4.10,P<0.001),SI(34.8277±20.9595vs.54.8049±25.0265,t=-9.39,P<0.001),and MADI(3.1741±1.9901vs.3.7289±2.7253,t=-2.74,P=0.012).The proposed CTGNet thus had significant advantages over the best traditional method on all evaluation metrics.Conclusion:The proposed A
基金This work was supported by the National Key Research and Development Program of China(2016YFC0905100)the National Natural Science Foundation of China(81770200)the Major Scientifc and Technological Projects for Collaborative Prevention and Control of Birth Defects in Hunan Province(20195K1010).
文摘Dear Editor,Nowadays,two autologous CAR19-T drugs,Tisagenlecleucel(Kymriah^(TM))and axicabtagene ciloleucel(Yescarta^(TM)),have been approved for the treatment of B cell leukemia and lymphoma and achieved unprecedented successes.However,about 10-20%of B-ALL patients receiving CAR19-T drugs didn't achieve complete remission(CR),while 30~50%of patients achieved CR would relapse mainly within 1 year.Moreover,the high CR rate of CAR19-T therapy for B-ALL can't be recaptured in other B-NHLs,'such as Burkitt's lymphoma(BL).Therefore,there is an urgent need to improve the therapeutic efficacy of CAR19-T cells.3.
