Wallerian degeneration （WD） remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely...Wallerian degeneration （WD） remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentiatly-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration.展开更多
Wallerian degeneration is an important area of research in modern neuroscience. A large number of genes are differentially regulated in the various stages of Wallerian degeneration, especially during the early respons...Wallerian degeneration is an important area of research in modern neuroscience. A large number of genes are differentially regulated in the various stages of Wallerian degeneration, especially during the early response. In this study, we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0, 0.5, 1,6, 12 and 24 hours after rat sciatic nerve injury using gene chip microarrays. We screened for differentially-expressed genes and gene expression patterns. We examined the data for Gene Ontology, and explored the Kyoto EncycLopedia of Genes and Genomes Pathway. This allowed us to identify key regulatory factors and recurrent network motifs. We identified 1 546 differentially-expressed genes and 21 distinct patterns ofgene expression in early Wallerian degeneration, and an enrichment of genes associated with the immune response, acute inflammation, apoptosis, cell adhesion, ion transport and the extracellular matrix. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-13, T cell receptor and calcium signaling pathways. Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine （C-C motif） ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral lAP repeat-containing 3 and Rac. The data were validated with real-time quantitative PCR. This study provides a global view of gene expression profiles in eady Wallerian degeneration of the rat sciatic nerve. Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration, and the regulation of nerve degeneration and regeneration.展开更多
1.Introduction High entropy alloys(HEAs),arising from the incorporation of multiple principal elements[1,2],have garnered extensive atten-tion due to their great potential to be utilized under extreme con-ditions[3-5].
[Objectives]To study the anti-inflammatory effects of extracts from different parts of Ficus pumila L.[Methods]Inflammatory models of xylene-induced ear swelling,glacial acetic acid-induced celiac capillary permeabili...[Objectives]To study the anti-inflammatory effects of extracts from different parts of Ficus pumila L.[Methods]Inflammatory models of xylene-induced ear swelling,glacial acetic acid-induced celiac capillary permeability and carrageenan-induced toe swelling were established to evaluate the effects of extracts from different parts of F.pumila L.on inflammation.The inflammatory model of ear swelling in mice with bilateral adrenalectomy induced by xylene was established to determine whether its anti-inflammatory effect depends on hypothalamus-pituitary-adrenal axis(HPAA)system.[Results]Compared with the blank control group,except that there was no statistical significance in the low dose group(P>0.05),the high and middle dose groups of extracts from different parts of F.pumila L.had significant inhibitory effects on xylene-induced ear swelling in mice.The high,middle and low dose groups of F.pumila L.extract could significantly increase the celiac capillary permeability of mice induced by glacial acetic acid,and significantly inhibit the toe swelling induced by carrageenan.Compared with the blank control group,the high and middle dose groups of extracts from different parts of F.pumila L.could significantly inhibit xylene-induced ear swelling and carrageenan-induced toe swelling in bilateral adrenalectomy mice.[Conclusions]The extracts from different parts of F.pumila L.had anti-inflammatory activity,and its anti-inflammatory effect was independent of hypothalamus-pituitary-adrenal axis(HPAA)system.展开更多
Based on the generalization and summary of the research on the functional constituents of chickpea at home and abroad in recent years,the research advances in some rich functional constituents including isoflavones,pr...Based on the generalization and summary of the research on the functional constituents of chickpea at home and abroad in recent years,the research advances in some rich functional constituents including isoflavones,proteins and peptides,carbohydrates,saponins and trace elements in chickpeas was reviewed in this paper. It provides a basis for the research,development and utilization of the functional constituents of chickpea in the future.展开更多
生淀粉酶可以在淀粉糊化温度以下的温度下直接降解生淀粉,具有巨大应用价值。丝状真菌生淀粉酶的产生受转录因子的严格调控。但是,草酸青霉(Penicillium oxalicum)中生淀粉酶产生的调控机制仍不清楚。前期工作中,通过比较基因组学获得...生淀粉酶可以在淀粉糊化温度以下的温度下直接降解生淀粉,具有巨大应用价值。丝状真菌生淀粉酶的产生受转录因子的严格调控。但是,草酸青霉(Penicillium oxalicum)中生淀粉酶产生的调控机制仍不清楚。前期工作中,通过比较基因组学获得了草酸青霉HP7-1中调控生淀粉酶产量的候选调控基因集。本研究以草酸青霉ΔPoxKu70为出发菌株,用同源重组技术敲除了其中一个候选调控基因POX03446,获得了缺失突变株ΔPOX03446。在可溶性淀粉培养条件下,与出发菌株ΔPoxKu70相比,转接后第2天,ΔPOX03446的生淀粉酶产量显著下降29.8%~40.3%(p<0.01;Student’s t test);转接后第4天,生淀粉酶产量显著下降14.6%~29.7%(p<0.01;Student’s t test),表明基因POX03446正向调控草酸青霉生淀粉酶的产生。NCBI BlastP比对分析显示POX03446与草酸青霉调控木聚糖酶基因和纤维素酶基因表达的转录因子PoXlnR一致性为46%。这是第一次报道POX03446调控草酸青霉生淀粉酶的产生。展开更多
[Objectives]To observe the effect of aqueous extract of Houttuynia cordata on the content of serum TNF-αand IL-6 in mice with abdominal infection caused by Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginos...[Objectives]To observe the effect of aqueous extract of Houttuynia cordata on the content of serum TNF-αand IL-6 in mice with abdominal infection caused by Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Proteus vulgaris and Klebsiella pneumoniae,so as to investigate the antibacterial effect of aqueous extract of Houttuynia cordata in vivo.[Methods]A total of 72 KM mice were randomly divided into six groups:model group,blank group,cefdinir dispersible tablet group(0.25 g/kg),high,medium and low dose groups of aqueous extract of Houttuynia cordata(40,20,10 g/kg crude drug).There were 12 mice in each group,half male and half female,continuously given intragastric administration for three days,once a day.The blank group and the model group were intragastrically infused with normal saline,and the other groups were given corresponding drugs with a volume of 0.02 mL/g.After 30 min of administration on the third day,the mice were injected intraperitoneally with 0.5 mL of bacterial suspension to make bacterial infection.The mice in the blank group were intraperitoneally injected with the same amount of normal saline,followed by intragastric administration 6 h after intraperitoneal injection and intragastric administration again after 24 h.The mice were observed for three days,and the changes of body weight before and after infection were recorded.On the fourth day of infection,eyeball blood was taken,serum was separated,and the concentration of inflammatory factors TNF-αand IL-6 in serum was determined.[Results]Compared with the model group,the high,medium and low dose groups of H.cordata aqueous extract had protective effects on mice with abdominal infection caused by S.aureus,E.coli,P.aeruginosa,P.vulgaris and K.pneumoniae,and decreased the content of TNF-αand IL-6 in serum.[Conclusions]The aqueous extract of H.cordata had a certain antibacterial effect on acute abdominal infection caused by S.aureus,E.coli,P.aeruginosa,P.vulgaris and K.pneumoniae,and its mechanism was related to TNF-αand IL-6.展开更多
Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands...Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.展开更多
[Objectives]This study was conducted to optimize the extraction process of rutin in Flos Sophorae.[Methods]With the percentage content of rutin extracted from Flos Sophorae as the evaluation index and rutin as the ref...[Objectives]This study was conducted to optimize the extraction process of rutin in Flos Sophorae.[Methods]With the percentage content of rutin extracted from Flos Sophorae as the evaluation index and rutin as the reference substance,the content of rutin in Flos Sophorae extracted ultrasonically was determined by high performance liquid chromatography.L_(9)(3^(4))orthogonal experiments were carried out to optimize the three factors(solvent concentration,material-to-liquid ratio,extraction time)that affect the effect of ultrasonic extraction of rutin from Flos Sophorae.[Results]The extraction effect of the crude medicinal powder was best with the methanol concentration of 80%,the ratio of material to liquid at 0.1∶30,and the extraction time of 40 min.[Conclusions]This study provides a reference for optimizing the extraction process of rutin in Flos Sophorae.展开更多
[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate metha...[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate methanol solution was used for color development,and the absorbance was measured at 516 nm wavelength;the mobile phase was methanol-0.1%phosphoric acid(76∶24)aqueous solution,the detection wavelength was set at 256 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample size was 10μL.[Results]The content of total anthraquinones in rhubarb was determined by UV spectrophotometry.The linear range was 0.98-23.52μg/mL,R2=0.9993,the average recoveries were 100.12%,99.66%and 99.15%,with RSDs of 1.58%,0.94%and 1.41%,respectively.The linear ranges of emodin,emodin,emodin,chrysophanol and emodin methyl ether were 0.0207-0.414(R2=0.9999),0.1721-3.442(R2=0.9996),0.0203-0.406(R2=0.9999),0.148-2.96(R2=0.9998)and 0.164-3.28(R2=0.9993),respectively.[Conclusions]The contents of total anthraquinones and free anthraquinones in different processed products of rhubarb were determined by UV spectrophotometry and HPLC.The method can be used for quality control of rhubarb and its different processed products.展开更多
[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the an...[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the analytical column,acetonitrile-water(55∶45)was used as the mobile phase,the flow rate was 0.5 mL/min,and the column temperature was 30℃and the detection wavelength was 306 nm.[Results]The linear range of the established serum sample analysis method was 1.0-20.0μg/mL,and the correlation coefficient was r=0.9994;the intraday and interday RSD of Wistar rat serum was less than 3.0%,and the accuracy was higher than 90%.[Conclusions]This method is sensitive,accurate,and rapid.It is suitable for monitoring the concentration of polydatin in serum after intragastric administration,and can also be used for pharmacokinetics and bioavailability studies.展开更多
基金supported by grants from the National Natural Science Foundation of China (81130080, 30870811)the Scientific Research Foundation for Returned Overseas Chinese Scholars, Ministry of Education of China+1 种基金the Natural Science Foundation of Jiangsu Province, China (BK2010282)the Priority Academic Program Development of Higher Education Institutions of Jiangsu Province, China
文摘Wallerian degeneration （WD） remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentiatly-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration.
基金supported by the National Natural Science Foundation of China (Key Program),No. 81130080the National Natural Science Foundation of China,No. 30870811+2 种基金Scientific Research Foundation for Returned Scholars,Ministry of Education of Chinathe Natural Science Foundation of Jiangsu Province,No. BK2010282A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,PAPD
文摘Wallerian degeneration is an important area of research in modern neuroscience. A large number of genes are differentially regulated in the various stages of Wallerian degeneration, especially during the early response. In this study, we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0, 0.5, 1,6, 12 and 24 hours after rat sciatic nerve injury using gene chip microarrays. We screened for differentially-expressed genes and gene expression patterns. We examined the data for Gene Ontology, and explored the Kyoto EncycLopedia of Genes and Genomes Pathway. This allowed us to identify key regulatory factors and recurrent network motifs. We identified 1 546 differentially-expressed genes and 21 distinct patterns ofgene expression in early Wallerian degeneration, and an enrichment of genes associated with the immune response, acute inflammation, apoptosis, cell adhesion, ion transport and the extracellular matrix. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-13, T cell receptor and calcium signaling pathways. Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine （C-C motif） ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral lAP repeat-containing 3 and Rac. The data were validated with real-time quantitative PCR. This study provides a global view of gene expression profiles in eady Wallerian degeneration of the rat sciatic nerve. Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration, and the regulation of nerve degeneration and regeneration.
基金National Natural Science Foundation of China(Nos.51971017,52271003,and 52071024)National Science Fund for Distinguished Young Scholars(No.52225103)+1 种基金Funds for Creative Research Groups of China(No.51921001)National Key Research and Development Program of China(No.2022YFB4602101).
文摘1.Introduction High entropy alloys(HEAs),arising from the incorporation of multiple principal elements[1,2],have garnered extensive atten-tion due to their great potential to be utilized under extreme con-ditions[3-5].
文摘[Objectives]To study the anti-inflammatory effects of extracts from different parts of Ficus pumila L.[Methods]Inflammatory models of xylene-induced ear swelling,glacial acetic acid-induced celiac capillary permeability and carrageenan-induced toe swelling were established to evaluate the effects of extracts from different parts of F.pumila L.on inflammation.The inflammatory model of ear swelling in mice with bilateral adrenalectomy induced by xylene was established to determine whether its anti-inflammatory effect depends on hypothalamus-pituitary-adrenal axis(HPAA)system.[Results]Compared with the blank control group,except that there was no statistical significance in the low dose group(P>0.05),the high and middle dose groups of extracts from different parts of F.pumila L.had significant inhibitory effects on xylene-induced ear swelling in mice.The high,middle and low dose groups of F.pumila L.extract could significantly increase the celiac capillary permeability of mice induced by glacial acetic acid,and significantly inhibit the toe swelling induced by carrageenan.Compared with the blank control group,the high and middle dose groups of extracts from different parts of F.pumila L.could significantly inhibit xylene-induced ear swelling and carrageenan-induced toe swelling in bilateral adrenalectomy mice.[Conclusions]The extracts from different parts of F.pumila L.had anti-inflammatory activity,and its anti-inflammatory effect was independent of hypothalamus-pituitary-adrenal axis(HPAA)system.
基金Supported by Key New Product Development Project of Yunnan Provincial of Science and Technology(2016BB002)Identification,Cataloguing and Propagation of Chickpea and Lupine Resources into National Crop Genebank of China(2018NWB036-07-B)
文摘Based on the generalization and summary of the research on the functional constituents of chickpea at home and abroad in recent years,the research advances in some rich functional constituents including isoflavones,proteins and peptides,carbohydrates,saponins and trace elements in chickpeas was reviewed in this paper. It provides a basis for the research,development and utilization of the functional constituents of chickpea in the future.
文摘生淀粉酶可以在淀粉糊化温度以下的温度下直接降解生淀粉,具有巨大应用价值。丝状真菌生淀粉酶的产生受转录因子的严格调控。但是,草酸青霉(Penicillium oxalicum)中生淀粉酶产生的调控机制仍不清楚。前期工作中,通过比较基因组学获得了草酸青霉HP7-1中调控生淀粉酶产量的候选调控基因集。本研究以草酸青霉ΔPoxKu70为出发菌株,用同源重组技术敲除了其中一个候选调控基因POX03446,获得了缺失突变株ΔPOX03446。在可溶性淀粉培养条件下,与出发菌株ΔPoxKu70相比,转接后第2天,ΔPOX03446的生淀粉酶产量显著下降29.8%~40.3%(p<0.01;Student’s t test);转接后第4天,生淀粉酶产量显著下降14.6%~29.7%(p<0.01;Student’s t test),表明基因POX03446正向调控草酸青霉生淀粉酶的产生。NCBI BlastP比对分析显示POX03446与草酸青霉调控木聚糖酶基因和纤维素酶基因表达的转录因子PoXlnR一致性为46%。这是第一次报道POX03446调控草酸青霉生淀粉酶的产生。
基金Project of Improving the Basic Scientific Research Ability of Young and Middle-aged Teachers in Guangxi Colleges and Universities in 2019(2019KY0341)Laboratory of Pharmaceutical Chemistry and Quality Analysis of Chinese(Zhuang)Medicine in the Third-level Scientific Research Laboratory of the State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa 200921).
文摘[Objectives]To observe the effect of aqueous extract of Houttuynia cordata on the content of serum TNF-αand IL-6 in mice with abdominal infection caused by Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Proteus vulgaris and Klebsiella pneumoniae,so as to investigate the antibacterial effect of aqueous extract of Houttuynia cordata in vivo.[Methods]A total of 72 KM mice were randomly divided into six groups:model group,blank group,cefdinir dispersible tablet group(0.25 g/kg),high,medium and low dose groups of aqueous extract of Houttuynia cordata(40,20,10 g/kg crude drug).There were 12 mice in each group,half male and half female,continuously given intragastric administration for three days,once a day.The blank group and the model group were intragastrically infused with normal saline,and the other groups were given corresponding drugs with a volume of 0.02 mL/g.After 30 min of administration on the third day,the mice were injected intraperitoneally with 0.5 mL of bacterial suspension to make bacterial infection.The mice in the blank group were intraperitoneally injected with the same amount of normal saline,followed by intragastric administration 6 h after intraperitoneal injection and intragastric administration again after 24 h.The mice were observed for three days,and the changes of body weight before and after infection were recorded.On the fourth day of infection,eyeball blood was taken,serum was separated,and the concentration of inflammatory factors TNF-αand IL-6 in serum was determined.[Results]Compared with the model group,the high,medium and low dose groups of H.cordata aqueous extract had protective effects on mice with abdominal infection caused by S.aureus,E.coli,P.aeruginosa,P.vulgaris and K.pneumoniae,and decreased the content of TNF-αand IL-6 in serum.[Conclusions]The aqueous extract of H.cordata had a certain antibacterial effect on acute abdominal infection caused by S.aureus,E.coli,P.aeruginosa,P.vulgaris and K.pneumoniae,and its mechanism was related to TNF-αand IL-6.
基金Supported by National Fund of Natural Sciences(No.30671456,30771503)Key Science & Technology Programsof Fujian Province(No.2006S1001)+1 种基金Fujian Fund of Natural Sciences(No.B0620004)Key S&T InnovationItems of Fujian Academy of Agricultural Sciences(2007)
文摘Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.
基金Supported by 2020 Guangxi University Middle-aged and Young Teachers'Basic Research Ability Improvement Project(No.2020KY13034)Scientific Research Task of Administration of Traditional Chinese Medicine of the Guangxi Zhuang Autonomous Region(No.GZZC2019147)+1 种基金the First Batch of High-level Talent Research Project in the Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(No.Y20196311)School-level Scientific Research Project of Youjiang Medical University for Nationalities(No.yy2018ky018)。
文摘[Objectives]This study was conducted to optimize the extraction process of rutin in Flos Sophorae.[Methods]With the percentage content of rutin extracted from Flos Sophorae as the evaluation index and rutin as the reference substance,the content of rutin in Flos Sophorae extracted ultrasonically was determined by high performance liquid chromatography.L_(9)(3^(4))orthogonal experiments were carried out to optimize the three factors(solvent concentration,material-to-liquid ratio,extraction time)that affect the effect of ultrasonic extraction of rutin from Flos Sophorae.[Results]The extraction effect of the crude medicinal powder was best with the methanol concentration of 80%,the ratio of material to liquid at 0.1∶30,and the extraction time of 40 min.[Conclusions]This study provides a reference for optimizing the extraction process of rutin in Flos Sophorae.
基金the Training Project of National Traditional Chinese Medicine Characteristic Technology Inheritance Talents(T20184828005,201548160100).
文摘[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate methanol solution was used for color development,and the absorbance was measured at 516 nm wavelength;the mobile phase was methanol-0.1%phosphoric acid(76∶24)aqueous solution,the detection wavelength was set at 256 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample size was 10μL.[Results]The content of total anthraquinones in rhubarb was determined by UV spectrophotometry.The linear range was 0.98-23.52μg/mL,R2=0.9993,the average recoveries were 100.12%,99.66%and 99.15%,with RSDs of 1.58%,0.94%and 1.41%,respectively.The linear ranges of emodin,emodin,emodin,chrysophanol and emodin methyl ether were 0.0207-0.414(R2=0.9999),0.1721-3.442(R2=0.9996),0.0203-0.406(R2=0.9999),0.148-2.96(R2=0.9998)and 0.164-3.28(R2=0.9993),respectively.[Conclusions]The contents of total anthraquinones and free anthraquinones in different processed products of rhubarb were determined by UV spectrophotometry and HPLC.The method can be used for quality control of rhubarb and its different processed products.
基金Project of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GZZC2019147)Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2020(2020KY13034)+2 种基金The First Batch High-level Talent Scientific Research Project of The Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311)National Traditional Chinese Medicine Characteristic Technology Heritage Talent Training Program(2015481601003)Program of Youjiang Medical University for Nationalities(yy2018ky018).
文摘[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the analytical column,acetonitrile-water(55∶45)was used as the mobile phase,the flow rate was 0.5 mL/min,and the column temperature was 30℃and the detection wavelength was 306 nm.[Results]The linear range of the established serum sample analysis method was 1.0-20.0μg/mL,and the correlation coefficient was r=0.9994;the intraday and interday RSD of Wistar rat serum was less than 3.0%,and the accuracy was higher than 90%.[Conclusions]This method is sensitive,accurate,and rapid.It is suitable for monitoring the concentration of polydatin in serum after intragastric administration,and can also be used for pharmacokinetics and bioavailability studies.
