In this paper,we provide a phylogenetic overview of Basidiomycota and related phyla in relation to ten years of DNA based phylogenetic studies since the AFTOL publications in 2007.We selected 529 species to address ph...In this paper,we provide a phylogenetic overview of Basidiomycota and related phyla in relation to ten years of DNA based phylogenetic studies since the AFTOL publications in 2007.We selected 529 species to address phylogenetic relationships of higher-level taxa using a maximumlikelihood framework and sequence data from six genes traditionally used in fungal molecular systematics(nrLSU,nrSSU,5.8S,tef1-a,rpb1 and rpb2).These species represent 18 classes,62 orders,183 families,and 392 genera from the phyla Basidiomycota(including the newly recognized subphylum Wallemiomycotina)and Entorrhizomycota,and 13 species representing 13 classes of Ascomycota as outgroup taxa.We also conducted a molecular dating analysis based on these six genes for 116 species representing 17 classes and 54 orders of Basidiomycota and Entorrhizomycota.Finally we performed a phyloproteomics analysis from 109 Basidiomycota species and 6 outgroup taxa using amino-acid sequences retrieved from 396 orthologous genes.Recognition of higher taxa follows the criteria in Zhao et al(Fungal Divers 78:239–292,2016):(i)taxa must be monophyletic and statistically well-supported in molecular dating analyses,(ii)their respective stem ages should be roughly equivalent,and(iii)stem ages of higher taxa must be older than those of lower level taxa.The time-tree indicates that the mean of stem ages of Basidiomycota and Entorrhizomycota are ca.530 Ma;subphyla of Basidiomycota are 406–490 Ma;most classes are 358–393 Ma for those of Agaricomycotina and 245–356 Ma for those of Pucciniomycotina and Ustilaginomycotina;most orders of those subphyla split 120–290 Ma.Monophyly of most higherlevel taxa of Basidiomycota are generally supported,especially those taxa introduced in the recent ten years:phylum Entorrhizomycota,classes Malasseziomycetes,Moniliellomycetes,Spiculogloeomycetes,Tritirachiomycetes and orders Amylocorticiales,Golubeviales,Holtermanniales,Jaapiales,Lepidostromatales,Robbauerales,Stereopsidales and Trichosporonales.However,the younger diverge展开更多
AIM:Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI...AIM:Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent p-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells. METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubGl population analysis, caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage. RESULTS: PEITC significantly induced apoptosis in HCT116 cells as assessed by SubGl population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described. CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the 展开更多
Handaxes are often regarded as the"tool-type-specimenoof the Acheulean techno-complex,not only because they are a very common artefact but because they contain rich technological,behavioral,and cognitive data tha...Handaxes are often regarded as the"tool-type-specimenoof the Acheulean techno-complex,not only because they are a very common artefact but because they contain rich technological,behavioral,and cognitive data that have been used to highlight trends in human evolution and lithic production strategies.To this end,the handaxe is the earliest tool possessing consistent morphological features,in particular,the appearance of a symmetrical concept in handaxe shape when compared with other Acheulean large shaping tools(e.g.,cleavers),and their manufacture is widely regarded as a significant milestone in the evolution of lithic technology,indicating improvements in tool-making hominins’abilities for complex thought and cognition.展开更多
We report a 27-year-old female who presented with abdominal distension, saddle anesthesia, and lower back pain. CT and MRI of the abdomen/spine were performed which showed a large anterior sacral meningocele occupying...We report a 27-year-old female who presented with abdominal distension, saddle anesthesia, and lower back pain. CT and MRI of the abdomen/spine were performed which showed a large anterior sacral meningocele occupying most of the pelvic and abdominal cavity and displacement of their respective contents. Anterior approach was chosen, uterine didelphys was identified and mobilized, meningocele identified and gentle drainage with subsequent resection of the cyst wall and watertight closure was achieved. Subsequent MRI demonstrated resolution of most the sacral meningocele. Patient symptoms improved and are doing well.展开更多
Novel analytical methods were developed for the determination of nitrapyrin and 6-chloropicolinic acid (6-CPA) residues in agricultural products. Nitrapyrin was extracted with hexane: toluene (1:1, v/v) and 6-CPA with...Novel analytical methods were developed for the determination of nitrapyrin and 6-chloropicolinic acid (6-CPA) residues in agricultural products. Nitrapyrin was extracted with hexane: toluene (1:1, v/v) and 6-CPA with 0.1 N sodium hydroxide (NaOH). For nitrapyrin, samples were cleaned with silica solid phase extraction (SPE) and for 6-CPA with C18 or carbon SPE. Analysis was performed by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) with external calibration (0.010 to 0.0010 µg∙mL−1 for nitrapyrin and 0.0050 to 0.00025 µg∙mL−1 for 6-CPA). Recoveries for nitrapyrin and 6-CPA were 68% - 102% and 66% - 90%, respectively. Nitrapyrin was detected in celery and onion (−1) and 6-CPA in onion, mustard greens and lettuce (−1). Lower level method validation (LLMV) for nitrapyrin and 6-CPA was 0.020 µg∙g−1 and 0.050 µg∙g−1, respectively. The methods presented in this study are robust and were utilized for the registration of nitrapyrin on agricultural commodities in the United States.展开更多
An accommodation-free displays,also known as Maxwellian displays,keep the displayed image sharp regardless of the viewer’s focal distance.However,they typically sufer from a small eye-box and limited efective feld of...An accommodation-free displays,also known as Maxwellian displays,keep the displayed image sharp regardless of the viewer’s focal distance.However,they typically sufer from a small eye-box and limited efective feld of view(FOV)which requires careful alignment before a viewer can see the image.Tis paper presents a high-quality accommodation-free head mounted display(aHMD)based on pixel beam scanning for direct image forming on retina.It has an enlarged eye-box and FOV for easy viewing by replicating the viewing points with an array of beam splitters.A prototype aHMD is built using this concept,which shows high defnition,low colour aberration 3D augmented reality(AR)images with an FOV of 36∘.Te advantage of the proposed design over other head mounted display(HMD)architectures is that,due to the narrow,collimated pixel beams,the high image quality is unafected by changes in eye accommodation,and the approach to enlarge the eye-box is scalable.Most importantly,such an aHMD can deliver realistic three-dimensional(3D)viewing perception with no vergence-accommodation confict(VAC).It is found that viewing the accommodation-free 3D images with the aHMD presented in this work is comfortable for viewers and does not cause the nausea or eyestrain side efects commonly associated with conventional stereoscopic 3D or HMD displays,even for all day use.展开更多
Traumatic brain injury remains a global health crisis that spans all demographics,yet there exist limited treatment options that may effectively curtail its lingering symptoms.Traumatic brain injury pathology entails ...Traumatic brain injury remains a global health crisis that spans all demographics,yet there exist limited treatment options that may effectively curtail its lingering symptoms.Traumatic brain injury pathology entails a progression from primary injury to inflammation-mediated secondary cell death.Sequestering this inflammation as a means of ameliorating the greater symptomology of traumatic brain injury has emerged as an attractive treatment prospect.In this review,we recapitulate and evaluate the important developments relating to regulating traumatic brain injury-induced neuroinflammation,edema,and blood-brain barrier disintegration through pharmacotherapy and stem cell transplants.Although these studies of stand-alone treatments have yielded some positive results,more therapeutic outcomes have been documented from the promising area of combined drug and stem cell therapy.Harnessing the facilitatory properties of certain pharmaceuticals with the anti-inflammatory and regenerative effects of stem cell transplants creates a synergistic effect greater than the sum of its parts.The burgeoning evidence in favor of combined drug and stem cell therapies warrants more elaborate preclinical studies on this topic in order to pave the way for later clinical trials.展开更多
Background:Wernicke encephalopathy is caused by thiamine(vitamin B1)defi ciency.It is generally considered to be a disease of adult alcoholics.However,it is known to occur in the pediatric population and in non-alcoho...Background:Wernicke encephalopathy is caused by thiamine(vitamin B1)defi ciency.It is generally considered to be a disease of adult alcoholics.However,it is known to occur in the pediatric population and in non-alcoholic conditions.Data sources:We searched PubMed with the key words Wernicke,thiamine,pediatric,children and adolescents and selected publications that were deemed appropriate.Results:The global prevalence rates of hunger,poverty and resultant nutrient deprivation have decreased in the 21st century.However,several scenarios which may predispose to Wernicke encephalopathy may be increasingly prevalent in children and adolescents such as malignancies,intensive care unit stays and surgical procedures for the treatment of obesity.Other predisposing conditions include magnesium defi ciency and defects in the SLC19A3 gene causing thiamine transporter-2 deficiency.The classic triad consists of encephalopathy,oculomotor dysfunction and gait ataxia but is not seen in a majority of patients.Treatment should be instituted immediately when the diagnosis is suspected clinically without waiting for laboratory confi rmation.Common magnetic resonance findings include symmetric T2 hyperintensities in dorsal medial thalamus,mammillary bodies,periaqueductal gray matter,and tectal plate.Conclusions:Wernicke encephalopathy is a medical emergency.Delay in its recognition and treatment may lead to significant morbidity,irreversible neurological damage or even death.This article aims to raise the awareness of this condition among pediatricians.展开更多
Background People living with HIV/AIDS(PLWHA)must contend with a significant burden of disease.However,current studies of this demographic have yielded wide variations in the incidence of suicidality(defined as suicid...Background People living with HIV/AIDS(PLWHA)must contend with a significant burden of disease.However,current studies of this demographic have yielded wide variations in the incidence of suicidality(defined as suicidal ideation,suicide attempt and suicide deaths).Aims This systematic review and meta-analysis aimed to assess the lifetime incidence and prevalence of suicidality in PLWHA.Methods Publications were identified from PubMed(MEDLINE),SCOPUS,OVID(MEDLINE),Joanna Briggs Institute EBP and Cochrane Library databases(from inception to before 1 February 2020).The search strategy included a combination of Medical Subject Headings associated with suicide and HIV.Researchers independently screened records,extracted outcome measures and assessed study quality.Data were pooled using a random-effects model.Subgroup and meta-regression analyses were conducted to explore the associated risk factors and to identify the sources of heterogeneity.Main outcomes were lifetime incidence of suicide completion and lifetime incidence and prevalence of suicidal ideation and suicide attempt.Results A total of 185199 PLWHA were identified from 40 studies(12 cohorts,27 cross-sectional and 1 nested case-control).The overall incidence of suicide completion in PLWHA was 10.2/1000 persons(95%CI:4.5 to 23.1),translating to 100-fold higher suicide deaths than the global general population rate of 0.11/1000 persons.The lifetime prevalence of suicide attempts was 158.3/1000 persons(95%CI:106.9 to 228.2)and of suicidal ideation was 228.3/1000 persons(95%CI:150.8 to 330.1).Meta-regression revealed that for every 10-percentage point increase in the proportion of people living with HIV with advanced disease(AIDS),the risk of suicide completion increased by 34 per 1000 persons.The quality of evidence by Grading of Recommendations,Assessment,Development and Evaluations for the suicide deaths was graded as‘moderate’quality.Conclusions The risk of suicide death is 100-fold higher in people living with HIV than in the general population.Lifetim展开更多
Objective:The objective of this study was to adapt an evidence-based smoking cessation intervention initially developed in the United States to the Chinese context and evaluate the ef-fectiveness of this intervention ...Objective:The objective of this study was to adapt an evidence-based smoking cessation intervention initially developed in the United States to the Chinese context and evaluate the ef-fectiveness of this intervention in China.Method:A smoking cessation program from United States was adapted and implemented through an Employee Assistance Program(EAP)in China.The intervention consisted of recom-mending medication and psychological counseling.Local EAP counselors received training on the program and delivered the intervention to clients.Program evaluation examined the intervention's effectiveness in helping clients quit or reduce the amount of smoking and nicotine dependence.Results:Ninety-day follow-up showed that those who had completed the program were more likely to remain abstinent,or stop smoking daily and have lower levels of nicotine dependence(p<0.05)than those who had dropped out.Conclusions:The evidence-based cessation program is effective in helping Chinese smok-ers quit or reduce the amount of smoking.Moreover,implementing such programs in an EAP setting is a practical approach to providing a wider spectrum of smokers with access to cessation assistance in China.展开更多
Paleogene sedimentary basins exposed across much of the central and eastern Tibetan Plateau may record the early history of plateau uplift related to the Indo\|Asian collision. We conducted sedimentological and strati...Paleogene sedimentary basins exposed across much of the central and eastern Tibetan Plateau may record the early history of plateau uplift related to the Indo\|Asian collision. We conducted sedimentological and stratigraphic investigations in the northeastern Qiangtang terrane, eastern Tibetan Plateau. Our results indicate development of several nonmarine basins during Paleogene(?) time, probably synchronous with northeast\|southwest contractional deformation. The Nangqian and Xialaxiu basins (96°～97°E, 32°～33°N) are composed of 500～ 20000m thick successions of primarily clastic sediment indicative of lacustrine and alluvial\|fan depositional processes. Paleocurrent measurements and sediment compositional data indicate local sediment source areas composed of Carboniferous\|Triassic carbonate and sandstone and minor Tertiary volcanic rocks. The large variability of provenance and facies types suggest that each basin evolved independently, as opposed to regional development of a single integrated basin which was partitioned by later deformation.展开更多
The biomass of wetland plants is highly responsive to environmental factors and plays a crucial role in the dynamics of the soil organic carbon(SOC)pool.In this study,we collected and analyzed global data on wetland p...The biomass of wetland plants is highly responsive to environmental factors and plays a crucial role in the dynamics of the soil organic carbon(SOC)pool.In this study,we collected and analyzed global data on wetland plant biomass from 1980 to 2021.By examining 1134 observations from 182 published papers on wetland ecosystems,we created a comprehensive database of wetland plant above-ground biomass(AGB)and below-ground biomass(BGB).Using this database,we analyzed the biomass characteristics of different climate zones,wetland types and plant species globally.Based on this,we analyzed the differences between the biomass of different plant species and the linkage between AGB and BGB and organic carbon.Our study has revealed that wetland plant AGB is greater in equatorial regions but BGB is highest in polar areas,and lowest in arid and equatorial zones.For plant species,the BGB of the Poales is higher than the AGB but Caryophyllales,Cyperales and Lamiales have higher AGB.Moreover,our findings indicate that BGB plays a more significant role in contributing to the organic carbon pool compared to AGB.Notably,when BGB is less than 1 t C ha^(-1),even slight changes in biomass can have a significant impact on the organic carbon pool.And we observed that the SOC increases by 5.7 t C ha^(-1)when the BGB content is low,indicating that the SOC is more sensitive to changes in biomass under such circumstances.Our study provides a basis for the global response of AGB and BGB of wetland plants to organic carbon.展开更多
The protection and management of the wetland should consider the changes in hydrological connectivity(HC)caused by the structural modifications of the soil macropores.The main purpose of our work is to clarify and qua...The protection and management of the wetland should consider the changes in hydrological connectivity(HC)caused by the structural modifications of the soil macropores.The main purpose of our work is to clarify and quantify the influence of the soil macropores volume on the vertical soil hydrodynamic process mechanically and statistically by taking the form of a case study in Yellow River Delta(YRD),and further reveal the vertical hydrological connectivity in this area.Based on X-ray computed tomography and constant head permeability test,the results showed a highly spatial heterogeneity of the soil structure in the YRD,hydraulic parameter(K_(s))was negatively correlated with bulk density and positively with soil macropore volume,soil aeration and maximum water capacity.Using Hydrus 1-D software and the Green–Ampt model,we estimated the characteristics of the hydrodynamic process in the soil without macropores,then evaluated the effect of the soil macropore on soil hydrodynamic process by comparing the experimental results with the simulation results.We found that increasing soil microporosity improved the convenience of water movement,which would enhance the HC of the region.The results will further help to reveal the eco-hydrological process at a vertical scale in soil and provide a theoretical guide for wetland conservation and restoration.展开更多
Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed t...Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 emission.Conclusions: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.展开更多
基金supported by grants from the National Natural Science Foundation of China to R.-L.Zhao(Project IDs 31470152 and 31360014)and G.-J.Li(Project ID 31500013)the Innovative Group of Edible Mushrooms Industry of Beijing(Project ID:BAIC05-2017)+2 种基金the Key Research and Development Program from Government of Guangxi Zhuang Autonomous Region(Project ID:2016AB05317)R.-L.Zhao,the Thailand Research Fund to K.D.Hyde(Grant BRG 5580009)the Natural Sciences and Engineering Research Council of Canada and the ROM Governors to J.-M.Moncalvo.
文摘In this paper,we provide a phylogenetic overview of Basidiomycota and related phyla in relation to ten years of DNA based phylogenetic studies since the AFTOL publications in 2007.We selected 529 species to address phylogenetic relationships of higher-level taxa using a maximumlikelihood framework and sequence data from six genes traditionally used in fungal molecular systematics(nrLSU,nrSSU,5.8S,tef1-a,rpb1 and rpb2).These species represent 18 classes,62 orders,183 families,and 392 genera from the phyla Basidiomycota(including the newly recognized subphylum Wallemiomycotina)and Entorrhizomycota,and 13 species representing 13 classes of Ascomycota as outgroup taxa.We also conducted a molecular dating analysis based on these six genes for 116 species representing 17 classes and 54 orders of Basidiomycota and Entorrhizomycota.Finally we performed a phyloproteomics analysis from 109 Basidiomycota species and 6 outgroup taxa using amino-acid sequences retrieved from 396 orthologous genes.Recognition of higher taxa follows the criteria in Zhao et al(Fungal Divers 78:239–292,2016):(i)taxa must be monophyletic and statistically well-supported in molecular dating analyses,(ii)their respective stem ages should be roughly equivalent,and(iii)stem ages of higher taxa must be older than those of lower level taxa.The time-tree indicates that the mean of stem ages of Basidiomycota and Entorrhizomycota are ca.530 Ma;subphyla of Basidiomycota are 406–490 Ma;most classes are 358–393 Ma for those of Agaricomycotina and 245–356 Ma for those of Pucciniomycotina and Ustilaginomycotina;most orders of those subphyla split 120–290 Ma.Monophyly of most higherlevel taxa of Basidiomycota are generally supported,especially those taxa introduced in the recent ten years:phylum Entorrhizomycota,classes Malasseziomycetes,Moniliellomycetes,Spiculogloeomycetes,Tritirachiomycetes and orders Amylocorticiales,Golubeviales,Holtermanniales,Jaapiales,Lepidostromatales,Robbauerales,Stereopsidales and Trichosporonales.However,the younger diverge
文摘AIM:Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent p-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells. METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubGl population analysis, caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage. RESULTS: PEITC significantly induced apoptosis in HCT116 cells as assessed by SubGl population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described. CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the
基金supported by the National Natural Science Foundation of China BSCTPES project(41988101)。
文摘Handaxes are often regarded as the"tool-type-specimenoof the Acheulean techno-complex,not only because they are a very common artefact but because they contain rich technological,behavioral,and cognitive data that have been used to highlight trends in human evolution and lithic production strategies.To this end,the handaxe is the earliest tool possessing consistent morphological features,in particular,the appearance of a symmetrical concept in handaxe shape when compared with other Acheulean large shaping tools(e.g.,cleavers),and their manufacture is widely regarded as a significant milestone in the evolution of lithic technology,indicating improvements in tool-making hominins’abilities for complex thought and cognition.
文摘We report a 27-year-old female who presented with abdominal distension, saddle anesthesia, and lower back pain. CT and MRI of the abdomen/spine were performed which showed a large anterior sacral meningocele occupying most of the pelvic and abdominal cavity and displacement of their respective contents. Anterior approach was chosen, uterine didelphys was identified and mobilized, meningocele identified and gentle drainage with subsequent resection of the cyst wall and watertight closure was achieved. Subsequent MRI demonstrated resolution of most the sacral meningocele. Patient symptoms improved and are doing well.
文摘Novel analytical methods were developed for the determination of nitrapyrin and 6-chloropicolinic acid (6-CPA) residues in agricultural products. Nitrapyrin was extracted with hexane: toluene (1:1, v/v) and 6-CPA with 0.1 N sodium hydroxide (NaOH). For nitrapyrin, samples were cleaned with silica solid phase extraction (SPE) and for 6-CPA with C18 or carbon SPE. Analysis was performed by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) with external calibration (0.010 to 0.0010 µg∙mL−1 for nitrapyrin and 0.0050 to 0.00025 µg∙mL−1 for 6-CPA). Recoveries for nitrapyrin and 6-CPA were 68% - 102% and 66% - 90%, respectively. Nitrapyrin was detected in celery and onion (−1) and 6-CPA in onion, mustard greens and lettuce (−1). Lower level method validation (LLMV) for nitrapyrin and 6-CPA was 0.020 µg∙g−1 and 0.050 µg∙g−1, respectively. The methods presented in this study are robust and were utilized for the registration of nitrapyrin on agricultural commodities in the United States.
基金The authors would like to thank the fnancial support of Huawei Technologies Co.,Ltd.,through the HIRP FLAGSHIP ProgramUK Engineering and Physical Sciences Research Council(EPSRC)through the EPSRC Centre for Innovative Manufacturing in Ultra Precision(EP/I033491/1).
文摘An accommodation-free displays,also known as Maxwellian displays,keep the displayed image sharp regardless of the viewer’s focal distance.However,they typically sufer from a small eye-box and limited efective feld of view(FOV)which requires careful alignment before a viewer can see the image.Tis paper presents a high-quality accommodation-free head mounted display(aHMD)based on pixel beam scanning for direct image forming on retina.It has an enlarged eye-box and FOV for easy viewing by replicating the viewing points with an array of beam splitters.A prototype aHMD is built using this concept,which shows high defnition,low colour aberration 3D augmented reality(AR)images with an FOV of 36∘.Te advantage of the proposed design over other head mounted display(HMD)architectures is that,due to the narrow,collimated pixel beams,the high image quality is unafected by changes in eye accommodation,and the approach to enlarge the eye-box is scalable.Most importantly,such an aHMD can deliver realistic three-dimensional(3D)viewing perception with no vergence-accommodation confict(VAC).It is found that viewing the accommodation-free 3D images with the aHMD presented in this work is comfortable for viewers and does not cause the nausea or eyestrain side efects commonly associated with conventional stereoscopic 3D or HMD displays,even for all day use.
基金CVB was funded by National Institutes of Health(NIH)R01NS071956,NIH R01NS090962,NIH R21NS089851,NIH R21NS094087Veterans Affairs Merit Review I01 BX001407
文摘Traumatic brain injury remains a global health crisis that spans all demographics,yet there exist limited treatment options that may effectively curtail its lingering symptoms.Traumatic brain injury pathology entails a progression from primary injury to inflammation-mediated secondary cell death.Sequestering this inflammation as a means of ameliorating the greater symptomology of traumatic brain injury has emerged as an attractive treatment prospect.In this review,we recapitulate and evaluate the important developments relating to regulating traumatic brain injury-induced neuroinflammation,edema,and blood-brain barrier disintegration through pharmacotherapy and stem cell transplants.Although these studies of stand-alone treatments have yielded some positive results,more therapeutic outcomes have been documented from the promising area of combined drug and stem cell therapy.Harnessing the facilitatory properties of certain pharmaceuticals with the anti-inflammatory and regenerative effects of stem cell transplants creates a synergistic effect greater than the sum of its parts.The burgeoning evidence in favor of combined drug and stem cell therapies warrants more elaborate preclinical studies on this topic in order to pave the way for later clinical trials.
基金Funding:The authors report receipt of no financial support for the research and/or authorship of this article
文摘Background:Wernicke encephalopathy is caused by thiamine(vitamin B1)defi ciency.It is generally considered to be a disease of adult alcoholics.However,it is known to occur in the pediatric population and in non-alcoholic conditions.Data sources:We searched PubMed with the key words Wernicke,thiamine,pediatric,children and adolescents and selected publications that were deemed appropriate.Results:The global prevalence rates of hunger,poverty and resultant nutrient deprivation have decreased in the 21st century.However,several scenarios which may predispose to Wernicke encephalopathy may be increasingly prevalent in children and adolescents such as malignancies,intensive care unit stays and surgical procedures for the treatment of obesity.Other predisposing conditions include magnesium defi ciency and defects in the SLC19A3 gene causing thiamine transporter-2 deficiency.The classic triad consists of encephalopathy,oculomotor dysfunction and gait ataxia but is not seen in a majority of patients.Treatment should be instituted immediately when the diagnosis is suspected clinically without waiting for laboratory confi rmation.Common magnetic resonance findings include symmetric T2 hyperintensities in dorsal medial thalamus,mammillary bodies,periaqueductal gray matter,and tectal plate.Conclusions:Wernicke encephalopathy is a medical emergency.Delay in its recognition and treatment may lead to significant morbidity,irreversible neurological damage or even death.This article aims to raise the awareness of this condition among pediatricians.
基金This work was supported by a U.S.National Institutes of Health(NIH)Director’s Transformative Award 1R01AI145057(PS)。
文摘Background People living with HIV/AIDS(PLWHA)must contend with a significant burden of disease.However,current studies of this demographic have yielded wide variations in the incidence of suicidality(defined as suicidal ideation,suicide attempt and suicide deaths).Aims This systematic review and meta-analysis aimed to assess the lifetime incidence and prevalence of suicidality in PLWHA.Methods Publications were identified from PubMed(MEDLINE),SCOPUS,OVID(MEDLINE),Joanna Briggs Institute EBP and Cochrane Library databases(from inception to before 1 February 2020).The search strategy included a combination of Medical Subject Headings associated with suicide and HIV.Researchers independently screened records,extracted outcome measures and assessed study quality.Data were pooled using a random-effects model.Subgroup and meta-regression analyses were conducted to explore the associated risk factors and to identify the sources of heterogeneity.Main outcomes were lifetime incidence of suicide completion and lifetime incidence and prevalence of suicidal ideation and suicide attempt.Results A total of 185199 PLWHA were identified from 40 studies(12 cohorts,27 cross-sectional and 1 nested case-control).The overall incidence of suicide completion in PLWHA was 10.2/1000 persons(95%CI:4.5 to 23.1),translating to 100-fold higher suicide deaths than the global general population rate of 0.11/1000 persons.The lifetime prevalence of suicide attempts was 158.3/1000 persons(95%CI:106.9 to 228.2)and of suicidal ideation was 228.3/1000 persons(95%CI:150.8 to 330.1).Meta-regression revealed that for every 10-percentage point increase in the proportion of people living with HIV with advanced disease(AIDS),the risk of suicide completion increased by 34 per 1000 persons.The quality of evidence by Grading of Recommendations,Assessment,Development and Evaluations for the suicide deaths was graded as‘moderate’quality.Conclusions The risk of suicide death is 100-fold higher in people living with HIV than in the general population.Lifetim
基金supported by an educational grant from Pfizer,Inc.
文摘Objective:The objective of this study was to adapt an evidence-based smoking cessation intervention initially developed in the United States to the Chinese context and evaluate the ef-fectiveness of this intervention in China.Method:A smoking cessation program from United States was adapted and implemented through an Employee Assistance Program(EAP)in China.The intervention consisted of recom-mending medication and psychological counseling.Local EAP counselors received training on the program and delivered the intervention to clients.Program evaluation examined the intervention's effectiveness in helping clients quit or reduce the amount of smoking and nicotine dependence.Results:Ninety-day follow-up showed that those who had completed the program were more likely to remain abstinent,or stop smoking daily and have lower levels of nicotine dependence(p<0.05)than those who had dropped out.Conclusions:The evidence-based cessation program is effective in helping Chinese smok-ers quit or reduce the amount of smoking.Moreover,implementing such programs in an EAP setting is a practical approach to providing a wider spectrum of smokers with access to cessation assistance in China.
文摘Paleogene sedimentary basins exposed across much of the central and eastern Tibetan Plateau may record the early history of plateau uplift related to the Indo\|Asian collision. We conducted sedimentological and stratigraphic investigations in the northeastern Qiangtang terrane, eastern Tibetan Plateau. Our results indicate development of several nonmarine basins during Paleogene(?) time, probably synchronous with northeast\|southwest contractional deformation. The Nangqian and Xialaxiu basins (96°～97°E, 32°～33°N) are composed of 500～ 20000m thick successions of primarily clastic sediment indicative of lacustrine and alluvial\|fan depositional processes. Paleocurrent measurements and sediment compositional data indicate local sediment source areas composed of Carboniferous\|Triassic carbonate and sandstone and minor Tertiary volcanic rocks. The large variability of provenance and facies types suggest that each basin evolved independently, as opposed to regional development of a single integrated basin which was partitioned by later deformation.
基金supported by the Fundamental Research Funds for the Central Universities(2022BLRD004 and BH2022-03)。
文摘The biomass of wetland plants is highly responsive to environmental factors and plays a crucial role in the dynamics of the soil organic carbon(SOC)pool.In this study,we collected and analyzed global data on wetland plant biomass from 1980 to 2021.By examining 1134 observations from 182 published papers on wetland ecosystems,we created a comprehensive database of wetland plant above-ground biomass(AGB)and below-ground biomass(BGB).Using this database,we analyzed the biomass characteristics of different climate zones,wetland types and plant species globally.Based on this,we analyzed the differences between the biomass of different plant species and the linkage between AGB and BGB and organic carbon.Our study has revealed that wetland plant AGB is greater in equatorial regions but BGB is highest in polar areas,and lowest in arid and equatorial zones.For plant species,the BGB of the Poales is higher than the AGB but Caryophyllales,Cyperales and Lamiales have higher AGB.Moreover,our findings indicate that BGB plays a more significant role in contributing to the organic carbon pool compared to AGB.Notably,when BGB is less than 1 t C ha^(-1),even slight changes in biomass can have a significant impact on the organic carbon pool.And we observed that the SOC increases by 5.7 t C ha^(-1)when the BGB content is low,indicating that the SOC is more sensitive to changes in biomass under such circumstances.Our study provides a basis for the global response of AGB and BGB of wetland plants to organic carbon.
基金supported by the Young Scientists Fund of the National Natural Science Foundation of China(42307573)Fundamental Research Funds for the Central Universities in China(BLX202250).
文摘The protection and management of the wetland should consider the changes in hydrological connectivity(HC)caused by the structural modifications of the soil macropores.The main purpose of our work is to clarify and quantify the influence of the soil macropores volume on the vertical soil hydrodynamic process mechanically and statistically by taking the form of a case study in Yellow River Delta(YRD),and further reveal the vertical hydrological connectivity in this area.Based on X-ray computed tomography and constant head permeability test,the results showed a highly spatial heterogeneity of the soil structure in the YRD,hydraulic parameter(K_(s))was negatively correlated with bulk density and positively with soil macropore volume,soil aeration and maximum water capacity.Using Hydrus 1-D software and the Green–Ampt model,we estimated the characteristics of the hydrodynamic process in the soil without macropores,then evaluated the effect of the soil macropore on soil hydrodynamic process by comparing the experimental results with the simulation results.We found that increasing soil microporosity improved the convenience of water movement,which would enhance the HC of the region.The results will further help to reveal the eco-hydrological process at a vertical scale in soil and provide a theoretical guide for wetland conservation and restoration.
基金supported by grants from the Natural Science Foundation of China (31272472)project grants from Meat & Livestock Australia,Fisheries and Forestry in Australia and Commonwealth Scientific and Industrial Research Organization (CSIRO)。
文摘Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 emission.Conclusions: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.
