Purpose: To evaluate the profile of in vitro susceptibility of yeasts isolated from cases of primary and espisodic vulvovaginitis to two antifungal agents. Methods: 40 Candida isolates from episodes of vulvovaginal ca...Purpose: To evaluate the profile of in vitro susceptibility of yeasts isolated from cases of primary and espisodic vulvovaginitis to two antifungal agents. Methods: 40 Candida isolates from episodes of vulvovaginal candidiasis were identified by classic methodologies. The susceptibility testing of the in vitro fluconazole and ketoconazole activity against the isolates was accessed by E-test. Results: C. albicans was the most common species identified in 70% of the occurrences followed by C. glabrata (20%), C. tropicalis (7.5%), and C. guilliermondii (2.5%). In the susceptibility profile to antifungal agents, 12.5% and 16.7% of the isolates obtained from primary and episodic vulvovaginal candidiasis were resistant to fluconazole, respectively. To ketoconazole, we found that 6.25% and 12.5% of the isolates respectively from primary vulvovaginal candidiasis (PVVC) and episodic vulvovaginal candidiasis (EVVC) had high MIC values. Conclusions: E-test is a reliable method for the susceptibility testing of Candida spp. due to its simplicity, reproducibility, and lack of specialized equipment. Resistant strains and non-albicans species were verified more in cases of EVVC than in PVVC. Clinical and mycological cure of patients with episodic vulvovaginal candidiasis or complicated cases occurred after prolonged treatment and sometimes with multiple antifungals use.展开更多
<b><span style="font-family:Verdana;">Aim</span></b><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "...<b><span style="font-family:Verdana;">Aim</span></b><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> To evaluate photodynamic therapy (PDT) </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> to reduce the growth of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp, and its synergy with the antifungals fluconazole and ketoconazole for inhibition of resistant, DDS and susceptible isolates from asymptomatic carriers and with complicated vulvovaginitis. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> Between 2017 and 2020</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> we evaluated 230 women with vulvovaginal candidiasis and 400 asymptomatic. We obtained 130 positive cultures for </span><i><span style="font-family:Verdana;">Candida </span></i><span style="font-family:Verdana;">spp from vulvovaginitis and 94 asymptomatic. Yeasts were characterized by classical and molecular tests. Sensitivity to fluconazole and ketoconazole was evaluated by E-test. We used photodynamic light through blue LED, wavelengths between 450 to 470 nm, power of 260 mW, energy</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">f</span><span style="font-family:Verdana;">l</span><span style="font-family:;" "=""><span style="font-family:Verdana;">uence of 270 J/cm</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, for 15 minutes over all colonies of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp</span></span><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">Methylene blue (MB) at 450 mg/mL, 2% gentian violet (VG) and 50</span><span style="font-family:;" "=""> </span><span style="font-fa展开更多
文摘Purpose: To evaluate the profile of in vitro susceptibility of yeasts isolated from cases of primary and espisodic vulvovaginitis to two antifungal agents. Methods: 40 Candida isolates from episodes of vulvovaginal candidiasis were identified by classic methodologies. The susceptibility testing of the in vitro fluconazole and ketoconazole activity against the isolates was accessed by E-test. Results: C. albicans was the most common species identified in 70% of the occurrences followed by C. glabrata (20%), C. tropicalis (7.5%), and C. guilliermondii (2.5%). In the susceptibility profile to antifungal agents, 12.5% and 16.7% of the isolates obtained from primary and episodic vulvovaginal candidiasis were resistant to fluconazole, respectively. To ketoconazole, we found that 6.25% and 12.5% of the isolates respectively from primary vulvovaginal candidiasis (PVVC) and episodic vulvovaginal candidiasis (EVVC) had high MIC values. Conclusions: E-test is a reliable method for the susceptibility testing of Candida spp. due to its simplicity, reproducibility, and lack of specialized equipment. Resistant strains and non-albicans species were verified more in cases of EVVC than in PVVC. Clinical and mycological cure of patients with episodic vulvovaginal candidiasis or complicated cases occurred after prolonged treatment and sometimes with multiple antifungals use.
文摘<b><span style="font-family:Verdana;">Aim</span></b><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> To evaluate photodynamic therapy (PDT) </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> to reduce the growth of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp, and its synergy with the antifungals fluconazole and ketoconazole for inhibition of resistant, DDS and susceptible isolates from asymptomatic carriers and with complicated vulvovaginitis. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> Between 2017 and 2020</span></span><span style="font-family:Verdana;">,</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> we evaluated 230 women with vulvovaginal candidiasis and 400 asymptomatic. We obtained 130 positive cultures for </span><i><span style="font-family:Verdana;">Candida </span></i><span style="font-family:Verdana;">spp from vulvovaginitis and 94 asymptomatic. Yeasts were characterized by classical and molecular tests. Sensitivity to fluconazole and ketoconazole was evaluated by E-test. We used photodynamic light through blue LED, wavelengths between 450 to 470 nm, power of 260 mW, energy</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">f</span><span style="font-family:Verdana;">l</span><span style="font-family:;" "=""><span style="font-family:Verdana;">uence of 270 J/cm</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, for 15 minutes over all colonies of </span><i><span style="font-family:Verdana;">Candida</span></i><span style="font-family:Verdana;"> spp</span></span><span style="font-family:Verdana;">. </span><span style="font-family:Verdana;">Methylene blue (MB) at 450 mg/mL, 2% gentian violet (VG) and 50</span><span style="font-family:;" "=""> </span><span style="font-fa
