Background Enhanced external counterpulsation (EECP) has been demonstrated to be effective in the treatment of patients with coronary artery disease (CAD). It has been proposed that the beneficial effects of EECP obse...Background Enhanced external counterpulsation (EECP) has been demonstrated to be effective in the treatment of patients with coronary artery disease (CAD). It has been proposed that the beneficial effects of EECP observed in clinical studies may be due to the formation of new blood vessels (angiogenesis) and collateral development. However, there is a relative paucity of basic studies to support the proposed mechanisms. Methods Twelve Beagle dogs were anesthetized with 3% sodium pentobarbital, 1 mg/kg intraperitoneal injection and mechanically ventilated for the development of myocardial infarction. After coronary occlusion, all animals were randomly assigned to either EECP or control. EECP was given one hour per day, 5 days a week, for a total of 28 to 30 hours treatment over a 6-week course. Immunohistochemical studies of alpha-actin and von Willebrand factor (vWF) were used to detect newly developed microvessels. Systemic and local vascular endothelial growth factor (VEGF) were identified by enzyme linked immunosorbent assay 9(ELISA) and reverse-transcriptional polymerase chain reaction (RT-PCR) analysis. Results There was a significant increase in the density of microvessels per mm(2) in the infarcted regions of EECP group compared to control group (vWF, 15.2 +/- 6.3 versus 4.9 +/- 2.1, P < 0.05; alpha-actin, 11.8 +/- 5.3 versus 3.4 +/- 1.2, P < 0.05), along with significant increase of positive vWF and a-actin stained area. Both immunohistochemical staining and RT-PCR analysis documented a significant increase in VEGF expression. These factors associated with angiogenesis corresponded to improved myocardial perfusion by Tc-99m-sestamibi single-photon emission computed tomography.展开更多
The Snowmelt Runoff Model (SRM) is one of a very few models in the world today that requires remote sensing derived snow cover as model input. Owing to its simple data requirements and use of remote sensing to provide...The Snowmelt Runoff Model (SRM) is one of a very few models in the world today that requires remote sensing derived snow cover as model input. Owing to its simple data requirements and use of remote sensing to provide snow cover information, SRM is ideal for use in data sparse regions, particularly in remote and inaccessible high mountain watersheds. In order to verify the applicability of SRM in an environment of continental climate, a test of SRM is performed for the Gongnaisi River basin in the western Tianshan Mountains, the results show that two SRM average goodness-of-fit statistics for simulations, Nash-Sutcliff coefficient (R2) and volume difference (DV), are 0.87 and 0.90%, respectively. As compared with the application results over 80 basins in 25 different countries around the world, SRM performs well in the Gongnaisi River basin. The results also show that SRM can be a validated snowmelt runoff model capable of being applied in the western Tianshan Mountains. On the basis of snowmelt runoff simulation, together with a set of simplified hypothetical climate scenarios, SRM is also used to simulate the effects of climate change on snow cover and the consecutive snowmelt runoff. For a given hypothetical temperature increase of 4℃, the snow coverage and snowmelt season shift towards earlier dates, and the snowmelt runoff, as a result, is changed significantly at the same time. The simulation results show that the snow cover is sensitive to changes of climate, especially to the increase of temperature, the major effect of climate change will be a time shifting of snowmelt runoff to early spring months, resulting in a redistribution of seasonally runoff throughout the whole snowmelt season.展开更多
A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with Co γ-Ray. Genetic analysis indicated that the 60 male-sterile phenotype was controlled by a single re...A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with Co γ-Ray. Genetic analysis indicated that the 60 male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells ex- panded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L lo- cus, an F2 population was constructed from the cross be- tween the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.展开更多
Background Chronic heart failure (CHF) is associated with calcium transients and calcium handling proteins. Angiotensin converting enzyme (ACE) inhibitor has been demonstrated to have beneficial effect on CHF. Yet st...Background Chronic heart failure (CHF) is associated with calcium transients and calcium handling proteins. Angiotensin converting enzyme (ACE) inhibitor has been demonstrated to have beneficial effect on CHF. Yet studies addressed to the relationship between ACE inhibitor and calcium transients in CHF are rare. The aim of this study was to investigate the influence of ACE inhibitor (perindopril) on the contractility and calcium transients and calcium handling proteins in ventricular myocytes from rats with experimental heart failure.Methods Male Wistar rats were randomized to heart failure group treated with perindopril (CHF-T, 3 mg·kg -1 ·d -1 ), heart failure group without treatment (CHF-C) and sham-operated group (PS). Heart failure was induced by abdominal aortic constriction. All groups were further followed up for 12 weeks. Left ventricular myocytes were then isolated. Single cell shortening fraction and [Ca 2+ ]_i were simultaneously measured by laser scanning confocal microscope under the field stimulation (1.0 Hz). Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to evaluate the changes of mRNA and protein of Na +-Ca 2+ exchanger (NCX_1), sarcoplasmic reticulum Ca 2+ -ATPase (SERCA_2) and phospholamban (PLB).Results The fraction of cell shortening (FS%) and [Ca 2+ ]_ imax (nmol/L) were significantly reduced in group CHF-C compared with group PS (FS%: 7.51±1.15 vs 13.21±1.49;[Ca 2+ ]_ imax :330.85±50.05 vs 498.16±14.07; both P <0.01), and restored at least partially in CHF-T group. In CHF-C group, the left ventricular mRNA of NCX_1 and PLB were significantly upregulated in comparing with PS group (R_ NCX1/β-Actin : 0.51±0.12 vs 0.19±0.06, P <0.01; R_ PLB/β-Actin : 0.26±0.12 vs 0.20±0.08, P <0.05), while SERCA_2 mRNA was downregulated (0.48±0.10 vs 0.80±0.11, P <0.01). The mRNA levels of NCX_1 and SERCA_2 in CHF-T group were between the CHF-C and PS group, and the differences of the latter two groups were significant (all 展开更多
This paper aims to identify problems in the teaching of tertiary extensive reading in China, and to recommend solutions to these problems. The author explores three major impediments to the teaching of extensive readi...This paper aims to identify problems in the teaching of tertiary extensive reading in China, and to recommend solutions to these problems. The author explores three major impediments to the teaching of extensive reading: a misconception about extensive reading, unsuitable reading material, and poor reading habits. Based on her years of teaching experience and extensive reading of literature works, she presents a new perspective and puts forward recommemtations ]'or removing these problems, including an adjustment to teaching goals, a change in reading material selection and an appropriate reading model. Meanwhile, the author recommends classroom-teaching practices that will benefit both teachers and students.展开更多
This paper is concerned with a high characteristic image processing and recognition system that is used for inspecting real-time blemishes, streaks and cracks on the inner walls of high accuracy pipes. As a regular de...This paper is concerned with a high characteristic image processing and recognition system that is used for inspecting real-time blemishes, streaks and cracks on the inner walls of high accuracy pipes. As a regular detector, the BP neural network is used for extracting features of the image inspected and classifying these images, it takes fully advantage of the function of artificial neural network, such as the information distributed memory, large scale self-adapting parallel processing, high fault-tolerant ability and so forth. Besides, an improved BP algorithm is used in the system for training the network, and making the learning procedure of the net converges to the minimum of overall situation at high rate.展开更多
The paper researched effect of adjuvants on activity and persistence of soil-applied herbicide Anwei.There were five adjuvants (fertility, Q7, oil, anionic and nonionic surfactants) in the experiment. We explored the ...The paper researched effect of adjuvants on activity and persistence of soil-applied herbicide Anwei.There were five adjuvants (fertility, Q7, oil, anionic and nonionic surfactants) in the experiment. We explored the influence of adjuvants on activity and persistence of Anwei by field trial. The results indicated that Q7, oil, anionic surfactant can increased activity of Anwei, and can lengthened its persistence for about five days.展开更多
文摘Background Enhanced external counterpulsation (EECP) has been demonstrated to be effective in the treatment of patients with coronary artery disease (CAD). It has been proposed that the beneficial effects of EECP observed in clinical studies may be due to the formation of new blood vessels (angiogenesis) and collateral development. However, there is a relative paucity of basic studies to support the proposed mechanisms. Methods Twelve Beagle dogs were anesthetized with 3% sodium pentobarbital, 1 mg/kg intraperitoneal injection and mechanically ventilated for the development of myocardial infarction. After coronary occlusion, all animals were randomly assigned to either EECP or control. EECP was given one hour per day, 5 days a week, for a total of 28 to 30 hours treatment over a 6-week course. Immunohistochemical studies of alpha-actin and von Willebrand factor (vWF) were used to detect newly developed microvessels. Systemic and local vascular endothelial growth factor (VEGF) were identified by enzyme linked immunosorbent assay 9(ELISA) and reverse-transcriptional polymerase chain reaction (RT-PCR) analysis. Results There was a significant increase in the density of microvessels per mm(2) in the infarcted regions of EECP group compared to control group (vWF, 15.2 +/- 6.3 versus 4.9 +/- 2.1, P < 0.05; alpha-actin, 11.8 +/- 5.3 versus 3.4 +/- 1.2, P < 0.05), along with significant increase of positive vWF and a-actin stained area. Both immunohistochemical staining and RT-PCR analysis documented a significant increase in VEGF expression. These factors associated with angiogenesis corresponded to improved myocardial perfusion by Tc-99m-sestamibi single-photon emission computed tomography.
基金supported by the National Natural Science Foundation of China(Grand No.40235053)Resources&Ecological Environment Key Projects of the Chinese Academy of Sciences(Grant No.kz951-b1-213).
文摘The Snowmelt Runoff Model (SRM) is one of a very few models in the world today that requires remote sensing derived snow cover as model input. Owing to its simple data requirements and use of remote sensing to provide snow cover information, SRM is ideal for use in data sparse regions, particularly in remote and inaccessible high mountain watersheds. In order to verify the applicability of SRM in an environment of continental climate, a test of SRM is performed for the Gongnaisi River basin in the western Tianshan Mountains, the results show that two SRM average goodness-of-fit statistics for simulations, Nash-Sutcliff coefficient (R2) and volume difference (DV), are 0.87 and 0.90%, respectively. As compared with the application results over 80 basins in 25 different countries around the world, SRM performs well in the Gongnaisi River basin. The results also show that SRM can be a validated snowmelt runoff model capable of being applied in the western Tianshan Mountains. On the basis of snowmelt runoff simulation, together with a set of simplified hypothetical climate scenarios, SRM is also used to simulate the effects of climate change on snow cover and the consecutive snowmelt runoff. For a given hypothetical temperature increase of 4℃, the snow coverage and snowmelt season shift towards earlier dates, and the snowmelt runoff, as a result, is changed significantly at the same time. The simulation results show that the snow cover is sensitive to changes of climate, especially to the increase of temperature, the major effect of climate change will be a time shifting of snowmelt runoff to early spring months, resulting in a redistribution of seasonally runoff throughout the whole snowmelt season.
基金This work Was supported by The National Basic Research Program of China(Grant No.TG1999011605)the Shanghai Municipal Committee of Science and Technology(Grant No.03DJ14016).
文摘A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with Co γ-Ray. Genetic analysis indicated that the 60 male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells ex- panded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L lo- cus, an F2 population was constructed from the cross be- tween the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.
文摘Background Chronic heart failure (CHF) is associated with calcium transients and calcium handling proteins. Angiotensin converting enzyme (ACE) inhibitor has been demonstrated to have beneficial effect on CHF. Yet studies addressed to the relationship between ACE inhibitor and calcium transients in CHF are rare. The aim of this study was to investigate the influence of ACE inhibitor (perindopril) on the contractility and calcium transients and calcium handling proteins in ventricular myocytes from rats with experimental heart failure.Methods Male Wistar rats were randomized to heart failure group treated with perindopril (CHF-T, 3 mg·kg -1 ·d -1 ), heart failure group without treatment (CHF-C) and sham-operated group (PS). Heart failure was induced by abdominal aortic constriction. All groups were further followed up for 12 weeks. Left ventricular myocytes were then isolated. Single cell shortening fraction and [Ca 2+ ]_i were simultaneously measured by laser scanning confocal microscope under the field stimulation (1.0 Hz). Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to evaluate the changes of mRNA and protein of Na +-Ca 2+ exchanger (NCX_1), sarcoplasmic reticulum Ca 2+ -ATPase (SERCA_2) and phospholamban (PLB).Results The fraction of cell shortening (FS%) and [Ca 2+ ]_ imax (nmol/L) were significantly reduced in group CHF-C compared with group PS (FS%: 7.51±1.15 vs 13.21±1.49;[Ca 2+ ]_ imax :330.85±50.05 vs 498.16±14.07; both P <0.01), and restored at least partially in CHF-T group. In CHF-C group, the left ventricular mRNA of NCX_1 and PLB were significantly upregulated in comparing with PS group (R_ NCX1/β-Actin : 0.51±0.12 vs 0.19±0.06, P <0.01; R_ PLB/β-Actin : 0.26±0.12 vs 0.20±0.08, P <0.05), while SERCA_2 mRNA was downregulated (0.48±0.10 vs 0.80±0.11, P <0.01). The mRNA levels of NCX_1 and SERCA_2 in CHF-T group were between the CHF-C and PS group, and the differences of the latter two groups were significant (all
文摘This paper aims to identify problems in the teaching of tertiary extensive reading in China, and to recommend solutions to these problems. The author explores three major impediments to the teaching of extensive reading: a misconception about extensive reading, unsuitable reading material, and poor reading habits. Based on her years of teaching experience and extensive reading of literature works, she presents a new perspective and puts forward recommemtations ]'or removing these problems, including an adjustment to teaching goals, a change in reading material selection and an appropriate reading model. Meanwhile, the author recommends classroom-teaching practices that will benefit both teachers and students.
文摘This paper is concerned with a high characteristic image processing and recognition system that is used for inspecting real-time blemishes, streaks and cracks on the inner walls of high accuracy pipes. As a regular detector, the BP neural network is used for extracting features of the image inspected and classifying these images, it takes fully advantage of the function of artificial neural network, such as the information distributed memory, large scale self-adapting parallel processing, high fault-tolerant ability and so forth. Besides, an improved BP algorithm is used in the system for training the network, and making the learning procedure of the net converges to the minimum of overall situation at high rate.
文摘The paper researched effect of adjuvants on activity and persistence of soil-applied herbicide Anwei.There were five adjuvants (fertility, Q7, oil, anionic and nonionic surfactants) in the experiment. We explored the influence of adjuvants on activity and persistence of Anwei by field trial. The results indicated that Q7, oil, anionic surfactant can increased activity of Anwei, and can lengthened its persistence for about five days.
