Despite widespread adoption and outstanding performance, machine learning models are considered as ‘‘blackboxes’’, since it is very difficult to understand how such models operate in practice. Therefore, in the po...Despite widespread adoption and outstanding performance, machine learning models are considered as ‘‘blackboxes’’, since it is very difficult to understand how such models operate in practice. Therefore, in the powersystems field, which requires a high level of accountability, it is hard for experts to trust and justify decisionsand recommendations made by these models. Meanwhile, in the last couple of years, Explainable ArtificialIntelligence (XAI) techniques have been developed to improve the explainability of machine learning models,such that their output can be better understood. In this light, it is the purpose of this paper to highlight thepotential of using XAI for power system applications. We first present the common challenges of using XAI insuch applications and then review and analyze the recent works on this topic, and the on-going trends in theresearch community. We hope that this paper will trigger fruitful discussions and encourage further researchon this important emerging topic.展开更多
The Hippo/Yap pathwayis a wll-conserved signaling cascade that regulates cell proliferation and dfferentiationto control organsize and stem/progenitor cell behavior.Following airway injury,Yap was dynamically regulate...The Hippo/Yap pathwayis a wll-conserved signaling cascade that regulates cell proliferation and dfferentiationto control organsize and stem/progenitor cell behavior.Following airway injury,Yap was dynamically regulated in regenerating airway epithelial clls.To deter.mine the role ofHippo signaling in the lung,the mammalian Hippo kinases,Mst1 and Mst2,were deleted in epithelial cells ofthe embry-onic and mature mouse lung.Mst1/2 deletion in the fetal lung enhanced proliferation and inhibited sacculation and epithelial cell differentiation.The transcriptional inhibition of cell proliferation and activation of differentiation during normal perinatal lung maturation were inversely regulated followving embryonic Mst1/2 deletion.Ablation of Mst1/2 from bronchiolar epithelial cells in the adult lung caused airway hyperplasia and altered differentiation.Inhibitory Yap phosphorylation was decreased and Yap nuclear localization and transcriptional targets were increased after Mst1/2 deletion,consistent with canonical Hippo/Yap signaling.YAP potentiated cell prolif-eration and inhibited dfferentiation of human bronchial epithelial cells in vitro.Loss of Mst1/2 and expression ofYAP regulated transcrip-tional targets controlling cell proliferation and diferentiation,including Ajuba LIM protein.Ajuba was required forthe effects ofYAP oncell proliferation in vitro.Hippo/Yapsignaling regulates Ajuba and controls proliferation and diferentiation oflung epithelial progenitor cells.展开更多
This perspective discussed the available evidence on the involvement of mTOR pathway in antiphospholipid syndrome(APS),from the aspects of endothelial cells,platelets,monocytes and anti-phospholipid antibodies(PLs),wh...This perspective discussed the available evidence on the involvement of mTOR pathway in antiphospholipid syndrome(APS),from the aspects of endothelial cells,platelets,monocytes and anti-phospholipid antibodies(PLs),which may lead to future therapeutic applications of mTOR inhibition in APS.展开更多
Advanced machine learning(ML)algorithms have outperformed traditional approaches in various forecasting applications,especially electricity price forecasting(EPF).However,the prediction accuracy of ML reduces substant...Advanced machine learning(ML)algorithms have outperformed traditional approaches in various forecasting applications,especially electricity price forecasting(EPF).However,the prediction accuracy of ML reduces substantially if the input data is not similar to the ones seen by the model during training.This is often observed in EPF problems when market dynamics change owing to a rise in fuel prices,an increase in renewable penetration,a change in operational policies,etc.While the dip in model accuracy for unseen data is a cause for concern,what is more,challenging is not knowing when the ML model would respond in such a manner.Such uncertainty makes the power market participants,like bidding agents and retailers,vulnerable to substantial financial loss caused by the prediction errors of EPF models.Therefore,it becomes essential to identify whether or not the model prediction at a given instance is trustworthy.In this light,this paper proposes a trust algorithm for EPF users based on explainable artificial intelligence techniques.The suggested algorithm generates trust scores that reflect the model’s prediction quality for each new input.These scores are formulated in two stages:in the first stage,the coarse version of the score is formed using correlations of local and global explanations,and in the second stage,the score is fine-tuned further by the Shapley additive explanations values of different features.Such score-based explanations are more straightforward than feature-based visual explanations for EPF users like asset managers and traders.A dataset from Italy’s and ERCOT’s electricity market validates the efficacy of the proposed algorithm.Results show that the algorithm has more than 85%accuracy in identifying good predictions when the data distribution is similar to the training dataset.In the case of distribution shift,the algorithm shows the same accuracy level in identifying bad predictions.展开更多
Wuschel-related homeobox （WOX） genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quick...Wuschel-related homeobox （WOX） genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOXgene family member from grape （Vitis vinifera L. cv. Cabernet Sauvignon）, and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem （SAM） of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L-1 2,4-D, 1 mg L-1 NAA and 1 mg L-1 BAP treatments, while downregulation was monitored by 1 mg L^-1 IBA treatment, and there was no response to 0.5 mg L-1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine.展开更多
Non-embryogenic calli （NEC） was inevitably and heavily produced when grape embryogenic calli （EC） was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform ...Non-embryogenic calli （NEC） was inevitably and heavily produced when grape embryogenic calli （EC） was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and EC.In this research,a vector pA5 containing a chitinase signal sequence fused to gfp （green fluorescent protein） and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera L.cv.Chardonnay NEC.It was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone （AS） was optimized as protocol.The use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher efficiency.Kanamycin at 200 mg L-1 was picked for positive expression selection.The stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques （RT-PCR） and fluorescent microscopy after three sub-cultures of the selected cell line.Highly efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for EC.This transformation platform could facilitate the verification of candidate somatic embryogenesis （SE） decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition.展开更多
<strong>Introduction</strong><strong>:</strong> The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent t...<strong>Introduction</strong><strong>:</strong> The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent team and is not easy to estimate by just watching matches. According to the specific tactical team behavior of “attack vs. defense” professional football matches are investigated based on a simulation approach, professional football matches are investigated according to the specific tactical team behavior of “attack vs. defense.” <strong>Methods:</strong> The formation patterns of all the sample games are categorized by SOCCER<span style="white-space:nowrap;">©</span> for defense and attack. Monte Carlo-Simulation can evaluate the mathematical, optimal strategy. The interaction simulation between attack and defense shows optimal flexibility rates for both tactical groups. <strong>Approach: </strong>A simulation approach based on 40 position data sets of the 2014/15 German Bundesliga has been conducted to analyze and optimize such strategic team behavior in professional soccer. <strong>Results:</strong> The results revealed that both attack and defense have optimal planning rates to be more successful. The more complex the success indicator, the more successful attacking player groups get. The results also show that defensive player groups always succeed in attacking groups below a specific planning rate value.<strong> Conclusion:</strong> Groups are always succeeding. The simulation-based position data analysis shows successful strategic behavior patterns for attack and defense. Attacking player groups need very high flexibility to be successful (stay in ball possession). In contrast, defensive player groups only need to be below a defined flexibility rate to be guaranteed more success.展开更多
Overstraining gun tubes has a twofold advantage. First, it enables the increase of the Safe Maximum Pressure(SMP) in the tube, resulting in a higher muzzle velocity which extends the gun's range and its projectile...Overstraining gun tubes has a twofold advantage. First, it enables the increase of the Safe Maximum Pressure(SMP) in the tube, resulting in a higher muzzle velocity which extends the gun's range and its projectile kinetic energy. Second, it reduces the tube's susceptibility to internal cracking which prolongs its fatigue life. Unfortunately, autofrettage also bears an inherent detrimental effect as it considerably increases the tensile hoop stress at the outer portion of the barrel's wall, which enhances external cracking of the tube by increasing the prevailing Stress Intensity Factor(SIF). In order to quantify this disadvantageous effect, 3-D Mode I SIFs distributions along the front of a single external radial semielliptical crack initiating from the outer surface of an autofrettaged modern gun barrel, overstrained by either the Swage or the Hydraulic autofrettage processes, are evaluated. The analysis is performed by the finite element(FE) method, using singular elements along the crack front. Innovative residual stress fields(RSFs), incorporating the Bauschinger effect for both types of autofrettage are applied to the barrel.Hill's [1] RSF is also applied to the tube for comparison reasons. All three RSFs are incorporated in the FE analysis, using equivalent temperature fields, Values for K_(IA)-the SIF resulting from the tensile residual stresses induced by autofrettage are evaluated for: a typical barrel of radii ratio R_o/R_i = 2, crack depth to wall-thickness ratios(a/t = 0.005-0.1),crack ellipticities(a/c = 0.2-1.0),and five levels of Swage,Hydraulic and Hill's autofrettage(e = 40%,60%,70%,80%,and 100%). In total,375 different 3-D cases are analyzed. The analysis demonstrates undoubtedly the detrimental effect of all types of autofrettage in increasing the prevailing effective stress intensity factor of external cracks, resulting in crack initiation enhancement and crack growth rate acceleration which considerably shortens the total fatigue life of the barrel. Nonetheless, the detrimental effect is auto展开更多
基金supported by Israel Science Foundation,grant No.1227/18.
文摘Despite widespread adoption and outstanding performance, machine learning models are considered as ‘‘blackboxes’’, since it is very difficult to understand how such models operate in practice. Therefore, in the powersystems field, which requires a high level of accountability, it is hard for experts to trust and justify decisionsand recommendations made by these models. Meanwhile, in the last couple of years, Explainable ArtificialIntelligence (XAI) techniques have been developed to improve the explainability of machine learning models,such that their output can be better understood. In this light, it is the purpose of this paper to highlight thepotential of using XAI for power system applications. We first present the common challenges of using XAI insuch applications and then review and analyze the recent works on this topic, and the on-going trends in theresearch community. We hope that this paper will trigger fruitful discussions and encourage further researchon this important emerging topic.
基金This work was supported by National Heart,Lung,and Blood Institute and Lung Repair and Re generation Consortium(LRRC)U01-110964 to JA.W.and A-K.PLung Map U01-HL122642 to JA.W.,B.R.S..and A-K.P.
文摘The Hippo/Yap pathwayis a wll-conserved signaling cascade that regulates cell proliferation and dfferentiationto control organsize and stem/progenitor cell behavior.Following airway injury,Yap was dynamically regulated in regenerating airway epithelial clls.To deter.mine the role ofHippo signaling in the lung,the mammalian Hippo kinases,Mst1 and Mst2,were deleted in epithelial cells ofthe embry-onic and mature mouse lung.Mst1/2 deletion in the fetal lung enhanced proliferation and inhibited sacculation and epithelial cell differentiation.The transcriptional inhibition of cell proliferation and activation of differentiation during normal perinatal lung maturation were inversely regulated followving embryonic Mst1/2 deletion.Ablation of Mst1/2 from bronchiolar epithelial cells in the adult lung caused airway hyperplasia and altered differentiation.Inhibitory Yap phosphorylation was decreased and Yap nuclear localization and transcriptional targets were increased after Mst1/2 deletion,consistent with canonical Hippo/Yap signaling.YAP potentiated cell prolif-eration and inhibited dfferentiation of human bronchial epithelial cells in vitro.Loss of Mst1/2 and expression ofYAP regulated transcrip-tional targets controlling cell proliferation and diferentiation,including Ajuba LIM protein.Ajuba was required forthe effects ofYAP oncell proliferation in vitro.Hippo/Yapsignaling regulates Ajuba and controls proliferation and diferentiation oflung epithelial progenitor cells.
文摘This perspective discussed the available evidence on the involvement of mTOR pathway in antiphospholipid syndrome(APS),from the aspects of endothelial cells,platelets,monocytes and anti-phospholipid antibodies(PLs),which may lead to future therapeutic applications of mTOR inhibition in APS.
文摘Advanced machine learning(ML)algorithms have outperformed traditional approaches in various forecasting applications,especially electricity price forecasting(EPF).However,the prediction accuracy of ML reduces substantially if the input data is not similar to the ones seen by the model during training.This is often observed in EPF problems when market dynamics change owing to a rise in fuel prices,an increase in renewable penetration,a change in operational policies,etc.While the dip in model accuracy for unseen data is a cause for concern,what is more,challenging is not knowing when the ML model would respond in such a manner.Such uncertainty makes the power market participants,like bidding agents and retailers,vulnerable to substantial financial loss caused by the prediction errors of EPF models.Therefore,it becomes essential to identify whether or not the model prediction at a given instance is trustworthy.In this light,this paper proposes a trust algorithm for EPF users based on explainable artificial intelligence techniques.The suggested algorithm generates trust scores that reflect the model’s prediction quality for each new input.These scores are formulated in two stages:in the first stage,the coarse version of the score is formed using correlations of local and global explanations,and in the second stage,the score is fine-tuned further by the Shapley additive explanations values of different features.Such score-based explanations are more straightforward than feature-based visual explanations for EPF users like asset managers and traders.A dataset from Italy’s and ERCOT’s electricity market validates the efficacy of the proposed algorithm.Results show that the algorithm has more than 85%accuracy in identifying good predictions when the data distribution is similar to the training dataset.In the case of distribution shift,the algorithm shows the same accuracy level in identifying bad predictions.
基金financially supported by the National Natural Science Foundation of China (30471212,30500347)the Beijing Natural Science Foundation(5992011)
文摘Wuschel-related homeobox （WOX） genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOXgene family member from grape （Vitis vinifera L. cv. Cabernet Sauvignon）, and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem （SAM） of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L-1 2,4-D, 1 mg L-1 NAA and 1 mg L-1 BAP treatments, while downregulation was monitored by 1 mg L^-1 IBA treatment, and there was no response to 0.5 mg L-1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine.
基金supported by the National Natural Science Foundation of China (30471212,30500347)the Earmarked Fund for Modern Agro-Industry Technology Research System,Ministry of Agriculture,China (NYCYTX-3-CY-04)
文摘Non-embryogenic calli （NEC） was inevitably and heavily produced when grape embryogenic calli （EC） was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and EC.In this research,a vector pA5 containing a chitinase signal sequence fused to gfp （green fluorescent protein） and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera L.cv.Chardonnay NEC.It was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone （AS） was optimized as protocol.The use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher efficiency.Kanamycin at 200 mg L-1 was picked for positive expression selection.The stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques （RT-PCR） and fluorescent microscopy after three sub-cultures of the selected cell line.Highly efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for EC.This transformation platform could facilitate the verification of candidate somatic embryogenesis （SE） decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition.
文摘<strong>Introduction</strong><strong>:</strong> The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent team and is not easy to estimate by just watching matches. According to the specific tactical team behavior of “attack vs. defense” professional football matches are investigated based on a simulation approach, professional football matches are investigated according to the specific tactical team behavior of “attack vs. defense.” <strong>Methods:</strong> The formation patterns of all the sample games are categorized by SOCCER<span style="white-space:nowrap;">©</span> for defense and attack. Monte Carlo-Simulation can evaluate the mathematical, optimal strategy. The interaction simulation between attack and defense shows optimal flexibility rates for both tactical groups. <strong>Approach: </strong>A simulation approach based on 40 position data sets of the 2014/15 German Bundesliga has been conducted to analyze and optimize such strategic team behavior in professional soccer. <strong>Results:</strong> The results revealed that both attack and defense have optimal planning rates to be more successful. The more complex the success indicator, the more successful attacking player groups get. The results also show that defensive player groups always succeed in attacking groups below a specific planning rate value.<strong> Conclusion:</strong> Groups are always succeeding. The simulation-based position data analysis shows successful strategic behavior patterns for attack and defense. Attacking player groups need very high flexibility to be successful (stay in ball possession). In contrast, defensive player groups only need to be below a defined flexibility rate to be guaranteed more success.
文摘Overstraining gun tubes has a twofold advantage. First, it enables the increase of the Safe Maximum Pressure(SMP) in the tube, resulting in a higher muzzle velocity which extends the gun's range and its projectile kinetic energy. Second, it reduces the tube's susceptibility to internal cracking which prolongs its fatigue life. Unfortunately, autofrettage also bears an inherent detrimental effect as it considerably increases the tensile hoop stress at the outer portion of the barrel's wall, which enhances external cracking of the tube by increasing the prevailing Stress Intensity Factor(SIF). In order to quantify this disadvantageous effect, 3-D Mode I SIFs distributions along the front of a single external radial semielliptical crack initiating from the outer surface of an autofrettaged modern gun barrel, overstrained by either the Swage or the Hydraulic autofrettage processes, are evaluated. The analysis is performed by the finite element(FE) method, using singular elements along the crack front. Innovative residual stress fields(RSFs), incorporating the Bauschinger effect for both types of autofrettage are applied to the barrel.Hill's [1] RSF is also applied to the tube for comparison reasons. All three RSFs are incorporated in the FE analysis, using equivalent temperature fields, Values for K_(IA)-the SIF resulting from the tensile residual stresses induced by autofrettage are evaluated for: a typical barrel of radii ratio R_o/R_i = 2, crack depth to wall-thickness ratios(a/t = 0.005-0.1),crack ellipticities(a/c = 0.2-1.0),and five levels of Swage,Hydraulic and Hill's autofrettage(e = 40%,60%,70%,80%,and 100%). In total,375 different 3-D cases are analyzed. The analysis demonstrates undoubtedly the detrimental effect of all types of autofrettage in increasing the prevailing effective stress intensity factor of external cracks, resulting in crack initiation enhancement and crack growth rate acceleration which considerably shortens the total fatigue life of the barrel. Nonetheless, the detrimental effect is auto
