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自交系BU-21/3——甜瓜(Cucumis meloL.)转基因育种的有效工具 被引量:2
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作者 M.galperin L.Patlis +7 位作者 A.Ovdia D.Wolf A.Zelcer D.Keingsbuch 李名旺 龙茂华 朱宗河 孔令传 《中国西瓜甜瓜》 2003年第4期26-27,共2页
甜瓜转化率低的问题仍具有挑战性。用1个新特质甜瓜自交系BU-21/3作材料研究其再生和转化能力,表明这个自交系的再生能力优于以往研究过的基因型。用含有报告基因GUS或GFP的根癌农杆菌浸染子叶外植体,研究瞬时表达和稳定表达效率,观察... 甜瓜转化率低的问题仍具有挑战性。用1个新特质甜瓜自交系BU-21/3作材料研究其再生和转化能力,表明这个自交系的再生能力优于以往研究过的基因型。用含有报告基因GUS或GFP的根癌农杆菌浸染子叶外植体,研究瞬时表达和稳定表达效率,观察到了大量瞬时表达,稳定表达时每个外植体可产生0.4~1.5个转基因芽,在转化后的8~10周,将转基因植株移入可控温室,发现表型和育性正常。再生能力的遗传分析表明它受单个显性位点控制,因此,这种新特质的基因型有望成为甜瓜转基因育种的有效工具。 展开更多
关键词 甜瓜 转基因育种 自交系BU-21/3 再生能力 报告基因 根癌农杆菌
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具有优良再生和转形能力的甜瓜基因型
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作者 M.galperin 谢国禄 《国外作物育种》 2004年第1期58-59,共2页
甜瓜育种中生物技术的利用引起人们较高的兴趣,并且生物技术可以为该物种的常规改良提供一种有价值的辅助方法。特别是广泛尝试了用编码有用性状的杂合基因来作了遗传转形,但是转形效率仍然较低,也被认为是一个难题。为了作转形研究... 甜瓜育种中生物技术的利用引起人们较高的兴趣,并且生物技术可以为该物种的常规改良提供一种有价值的辅助方法。特别是广泛尝试了用编码有用性状的杂合基因来作了遗传转形,但是转形效率仍然较低,也被认为是一个难题。为了作转形研究,通常在子叶和幼叶衍生的外植体中使用农业细菌媒介作基因转移者。 展开更多
关键词 甜瓜 基因型 再生能力 转形能力 BU-21/3品系 转基因育种
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A Validated Model for the Imaging Diagnosis of Cystic Lung Disease
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作者 Wallace T. Miller Karen C. Patterson +22 位作者 Shweta Sood James E. Schmitt Arshad A. Wani Robert Borden Maya galperin-Aisenberg Mary K. Porteus Michelle L. Hershman Michael Hewitt Jennifer Levy Victor D. Babatunde Tetiana Glushko Timothy J. Niesen Sergey Leshchinskiy Karine Sahakyan Keyur Desai Jennifer A. Gillman Sandeep Reddy Michael Shriver Nathaniel B. Linna Abass M. Noor Aysenur Buz Matthew E. Biron Scott Simpson 《Open Journal of Radiology》 2023年第1期42-57,共16页
Rationale and Objectives: Cystic lung disease may be accurately diagnosed by imaging interpretation of specialist radiologists, without other information. We hypothesized that with minimal training non-specialists cou... Rationale and Objectives: Cystic lung disease may be accurately diagnosed by imaging interpretation of specialist radiologists, without other information. We hypothesized that with minimal training non-specialists could perform similarly to specialist physicians in the diagnosis of cystic lung disease. Methods: 72 cystic lung disease cases and 25 cystic lung disease mimics were obtained from three sources: 1) a prospective acquired diffuse lung disease registry, 2) a retrospective search of medical records and 3) teaching files. Cases were anonymized, randomized and interpreted by 7 diffuse lung disease specialists and 15 non-specialist radiologists and pulmonologists. Clinical information other than age and sex was not provided. Prior to interpretation, non-specialists viewed a short PDF training document explaining cystic lung disease interpretation. Results: Correct first choice diagnosis of 85%-88% may be achieved by high-performing specialist readers and 71%-80% by non-specialists and lower-performing specialists, with mean accuracies in the diagnosis of LAM (91%, p Conclusion: With specific but limited training, non-specialist physicians can diagnose cystic lung diseases from CT appearance alone with similar accuracy to specialists, correctly identifying approximately 75% of cases. 展开更多
关键词 LYMPHANGIOLEIOMYOMATOSIS HISTIOCYTOSIS Langerhans-Cell Idiopathic Interstitial Pneumonias Birt-Hogg-Dube Syndrome Lung Diseases INTERSTITIAL DIAGNOSES Differential
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High Moisture Sensitivity of the Elements Based on Carbon Nanotubes Array
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作者 Sergei Bulyarsky Vyacheslav galperin +3 位作者 Levan Ichkitidze Michael Ermakov Alexander Pavlov Yuri Shaman 《Materials Sciences and Applications》 2013年第5期8-10,共3页
The results of the research and development of the moisture-sensitive elements based on the carbon nanotubes (CNT) array are presented. It was shown that CNT arrays that were grown by low-temperature plasma enhanced c... The results of the research and development of the moisture-sensitive elements based on the carbon nanotubes (CNT) array are presented. It was shown that CNT arrays that were grown by low-temperature plasma enhanced chemical vapor deposition (PECVD) method on the planar Si structures exhibit extremely high moisture sensitivity. The structure resistance ratio in dry and moisture conditions exceed 400. Such relatively high change in resistances is conditioned by the pattern of change of the charge carrier’s conductivity between certain nanotubes in the bundle when water molecules adsorption occurs. 展开更多
关键词 Carbon NANOTUBES Moisture-Sensitive ELEMENTS CONDUCTIVITY MOLECULES Adsorption
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Electrically-Conductive Composite Nanomaterial with Multi-Walled Carbon Nanotubes
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作者 Levan Ichkitidze Vitally Podgaetsky +5 位作者 Sergei Selishchev Eugenie Blagov Vyacheslav galperin Yuri Shaman Alexander Pavlov Eugenie Kitsyuk 《Materials Sciences and Applications》 2013年第5期1-7,共7页
Specific conductivity of the composite nanomaterial layers with micron and submicron dimensions, consisting of carboxymethyl cellulose (CMC) and multiwalled carbon nanotubes (MWCNT) was investigated. Ultradispersed aq... Specific conductivity of the composite nanomaterial layers with micron and submicron dimensions, consisting of carboxymethyl cellulose (CMC) and multiwalled carbon nanotubes (MWCNT) was investigated. Ultradispersed aqueous suspension was deposited on soft (aluminum foil, plates made from polyester and polyimide, cotton fabric, office paper) and solid (coverslip, silicon wafers with silicon oxide layer) substrates by silk-screen printing. Electrical resistance was measured by four-probe method and by the method of square on surface from which the conductivity and conductivity per square of surface were calculated taking into account layer’s geometric dimensions. Specific conductivity of the layers with thickness range 0.5 - 5 μm was? ~1.2×104÷4×104 S/m, and max conductivity per square was ~ 0.2 S. Investigated nanomaterial is attractive to electronic and biomedical applications. 展开更多
关键词 Multiwalled Carbon NANOTUBES Electroconductive NANOMATERIAL Substrates CARBOXYMETHYL CELLULOSE Electroconductive GEL Electroconductive Layers
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Bulk Composite Nanomaterial with Multiwall Carbon Nanotubes
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作者 Levan Ichkitidze Vitally Podgaetsky +5 位作者 Alexander Prihodko Sergei Selishchev Eugenie Blagov Vyacheslav galperin Yuri Shaman Lyudmila Tabulina 《Materials Sciences and Applications》 2012年第10期728-732,共5页
The properties of the composite nanomaterials (CNM) based on bovine serum albumin (BSA) and multi-walled carbon nanotubes (MWCNT), both functionalized and non-functionalized, were investigated. In order to obtain the ... The properties of the composite nanomaterials (CNM) based on bovine serum albumin (BSA) and multi-walled carbon nanotubes (MWCNT), both functionalized and non-functionalized, were investigated. In order to obtain the solid-state bulk CNM from the ultradispersed aqueous solutions of 25 wt.% BSA and (0.0015 - 0.04) wt.% MWCNT, the methods of nanotechnology and laser technology were used. It is revealed that the CNM density is 10% - 20% higher than that of water and the hardness is higher than that of BSA by a factor of 3 - 6 times. An increase in hardness Hv (by Vickers) of CNM correlated with an increase in the concentration of MWCNT, and Hyreached ~300 MPa for the case of the non-functionalized MWCNT, while for the case of the functionalized MWCNT, i.e. MWCNTf, Hy was 25% lower. 展开更多
关键词 CARBON NANOTUBES FUNCTIONALIZATION Albumine BIOCOMPATIBLE COMPOSITE NANOMATERIAL
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