Objective:The aim of the present study was to isolate the anti-MRSA(Methicillin Resistant Staphylococcus aureus)molecule from the Mangrove symbiont Streptomyces and its biomedical studies in Zebrafish embryos.Methods:...Objective:The aim of the present study was to isolate the anti-MRSA(Methicillin Resistant Staphylococcus aureus)molecule from the Mangrove symbiont Streptomyces and its biomedical studies in Zebrafish embryos.Methods:MRSA was isolated from the pus samples of Colachal hospitals and confirmed by amplification of mecA gene.Anti-MRSA molecule producing strain was identified by!6s rRNA gene sequencing.Anti-MRSA compound production was optimized by Solid State Fermentation(SSF)and the purification of the active molecule was carried out by TLC and RP-HPLC.The inhibitory concentration and LC_(50)were calculated using Statistical software SPSS.The Biomedical studies including the cardiac assay and organ toxicity assessment were carried out in Zebraiish.Results:The bioactive anti-MRSA small molecule A,was purified by TLC with Rf value of 0.37 with 1.389 retention time at RP-HPLC.The Inhibitory Concentration of the purified molecule A_2 was 30μg/mL but,the inhibitory concentration of the MRSA in the infected embryo was 32-34μg/mL for TLC purified molecule A,with LC_(50)mean value was61.504μg/mL.Zebrafish toxicity was assessed in 48-60μg/mL by observing the physiological deformities and the heart beat rates(HBR)of embryos for anti MRSA molecule showed the mean of 41.33-41.67 HBR/15 seconds for 40μg/mL and control was 42.33-42.67 for 15 seconds which significantly showed that the anti-MRSA molecule A_2 did not affected the HBR.Conclusions:Anti-MRSA molecule from Streptomyces sp PVRK-I was isolated and biomedical studies in Zebrafish model assessed that the molecule was non toxic at the minimal inhibitory concentration of MRSA.展开更多
Permanent magnet brushless DC motors are used for various low-power applications,namely domestic fans,washing machines,mixer grinders and cooling fan applications.This paper focuses on selecting the best laminating ma...Permanent magnet brushless DC motors are used for various low-power applications,namely domestic fans,washing machines,mixer grinders and cooling fan applications.This paper focuses on selecting the best laminating material for the interior permanent magnet brushless DC(IPM BLDC)motor used in the cooling fan of automobiles.Various laminating materials,namely M19-29GA,M800-65A and M43,are tested using finite element analysis.The machine's vital performance metrics,namely the stator current,torque ripple,and hysteresis loss were analyzed in selecting the laminating material.The designed motor is also modelled as a mathematical model from the computed lumped parameters.The performance of the machines was validated through electromagnetic and thermal analysis.展开更多
Surgical resection of mandible owing to benign,malignant neoplasm,osteoradionecrosis is common. The resection can be total or segmental depending on the lesion. Loss of mandibular continuity causes deviation of remain...Surgical resection of mandible owing to benign,malignant neoplasm,osteoradionecrosis is common. The resection can be total or segmental depending on the lesion. Loss of mandibular continuity causes deviation of remaining mandibular segment towards the resected side and rotation inferiorly due to muscle pull and scar contracture affecting mastication and esthetics. Surgical reconstruction may not be always possible. Prosthetic rehabilitation plays a major role in these patients. This case series describes different types of guiding flange(GF) prosthesis with modifications for three hemimandibulectomy patients at different time interval after surgery. The article details GF prosthesis combined with physiotherapy to correct deviation of mandible thereby improving mastication,esthetics and speech and thus enhancing the quality of life.展开更多
Obective:To evaluate the larvieidal.ovieidal and repellent properties of solvent extracts of marine sponge Cliona celala(C.celata)(Grant) against the malarial vector Anopheles stephensi(An.stephensi) Listen.Methods:Ma...Obective:To evaluate the larvieidal.ovieidal and repellent properties of solvent extracts of marine sponge Cliona celala(C.celata)(Grant) against the malarial vector Anopheles stephensi(An.stephensi) Listen.Methods:Marine sponge C.celala was thoroughly washed with distilled water and shade dried for 48 h.Then the sponges were homogenized and extracted sequentially with hexane,ethyl acetate and methanol,Lanvicidal and ovicidal activities were tested at four different concentrations viz.,62.5.125.0.250.0 and 500.0 ppm.for repellent study extracts were taken in three different concentrations viz..5.0.2.5.1.0 mg/cm at.Results:Among the three solvent extracts of C.celata,methanol extract showed thc highest lanvieidal activity at 500 ppm against the fourth inslar larvae of An.stephensi.The LC_(50)and LC_(90) values of C.celata methanol extract were recorded as 80.61 and 220.81 ppm against An.stephensi larvae respectively.High ovieidal activity of 91.2%was recorded al 500 ppm concentration of methanol extract.The haxane extract was found to be the most effective protectant against the adult female mosquitoes of An.stephensi.The mean protection time recorded in hexane extract was up to 245 min at 5 mg/cm^2 dosage against An.Stephensi adults.Conclusions:The screening results suggest that the hexane and methanol extracts of C.celata arc promising in mosquito control.Considering these bioaclivitics.C.celata could the probed further to obtain some novel pesticidal molecules.展开更多
基金Supported by Xpression Biotek Ltd.and International Centre for Nanobiotechnology(ICN).Manonmaniam Sundaranar University
文摘Objective:The aim of the present study was to isolate the anti-MRSA(Methicillin Resistant Staphylococcus aureus)molecule from the Mangrove symbiont Streptomyces and its biomedical studies in Zebrafish embryos.Methods:MRSA was isolated from the pus samples of Colachal hospitals and confirmed by amplification of mecA gene.Anti-MRSA molecule producing strain was identified by!6s rRNA gene sequencing.Anti-MRSA compound production was optimized by Solid State Fermentation(SSF)and the purification of the active molecule was carried out by TLC and RP-HPLC.The inhibitory concentration and LC_(50)were calculated using Statistical software SPSS.The Biomedical studies including the cardiac assay and organ toxicity assessment were carried out in Zebraiish.Results:The bioactive anti-MRSA small molecule A,was purified by TLC with Rf value of 0.37 with 1.389 retention time at RP-HPLC.The Inhibitory Concentration of the purified molecule A_2 was 30μg/mL but,the inhibitory concentration of the MRSA in the infected embryo was 32-34μg/mL for TLC purified molecule A,with LC_(50)mean value was61.504μg/mL.Zebrafish toxicity was assessed in 48-60μg/mL by observing the physiological deformities and the heart beat rates(HBR)of embryos for anti MRSA molecule showed the mean of 41.33-41.67 HBR/15 seconds for 40μg/mL and control was 42.33-42.67 for 15 seconds which significantly showed that the anti-MRSA molecule A_2 did not affected the HBR.Conclusions:Anti-MRSA molecule from Streptomyces sp PVRK-I was isolated and biomedical studies in Zebrafish model assessed that the molecule was non toxic at the minimal inhibitory concentration of MRSA.
文摘Permanent magnet brushless DC motors are used for various low-power applications,namely domestic fans,washing machines,mixer grinders and cooling fan applications.This paper focuses on selecting the best laminating material for the interior permanent magnet brushless DC(IPM BLDC)motor used in the cooling fan of automobiles.Various laminating materials,namely M19-29GA,M800-65A and M43,are tested using finite element analysis.The machine's vital performance metrics,namely the stator current,torque ripple,and hysteresis loss were analyzed in selecting the laminating material.The designed motor is also modelled as a mathematical model from the computed lumped parameters.The performance of the machines was validated through electromagnetic and thermal analysis.
基金Department of surgical oncology,Government Royapettah hospital,Chennai,Indiathe Department of Plastic surgery,Rajiv Gandhi Government General hospital,Chennai,India for their valuable support
文摘Surgical resection of mandible owing to benign,malignant neoplasm,osteoradionecrosis is common. The resection can be total or segmental depending on the lesion. Loss of mandibular continuity causes deviation of remaining mandibular segment towards the resected side and rotation inferiorly due to muscle pull and scar contracture affecting mastication and esthetics. Surgical reconstruction may not be always possible. Prosthetic rehabilitation plays a major role in these patients. This case series describes different types of guiding flange(GF) prosthesis with modifications for three hemimandibulectomy patients at different time interval after surgery. The article details GF prosthesis combined with physiotherapy to correct deviation of mandible thereby improving mastication,esthetics and speech and thus enhancing the quality of life.
基金Entomology Research Institute,Loyola College for financial assistance
文摘Obective:To evaluate the larvieidal.ovieidal and repellent properties of solvent extracts of marine sponge Cliona celala(C.celata)(Grant) against the malarial vector Anopheles stephensi(An.stephensi) Listen.Methods:Marine sponge C.celala was thoroughly washed with distilled water and shade dried for 48 h.Then the sponges were homogenized and extracted sequentially with hexane,ethyl acetate and methanol,Lanvicidal and ovicidal activities were tested at four different concentrations viz.,62.5.125.0.250.0 and 500.0 ppm.for repellent study extracts were taken in three different concentrations viz..5.0.2.5.1.0 mg/cm at.Results:Among the three solvent extracts of C.celata,methanol extract showed thc highest lanvieidal activity at 500 ppm against the fourth inslar larvae of An.stephensi.The LC_(50)and LC_(90) values of C.celata methanol extract were recorded as 80.61 and 220.81 ppm against An.stephensi larvae respectively.High ovieidal activity of 91.2%was recorded al 500 ppm concentration of methanol extract.The haxane extract was found to be the most effective protectant against the adult female mosquitoes of An.stephensi.The mean protection time recorded in hexane extract was up to 245 min at 5 mg/cm^2 dosage against An.Stephensi adults.Conclusions:The screening results suggest that the hexane and methanol extracts of C.celata arc promising in mosquito control.Considering these bioaclivitics.C.celata could the probed further to obtain some novel pesticidal molecules.
