In this paper, we propose a mechanism named modified backoff （MB） mechanism to decrease the channel idle time in IEEE 802.11 distributed coordination function （DCF）. In the noisy channel, when signal-to-noise rat...In this paper, we propose a mechanism named modified backoff （MB） mechanism to decrease the channel idle time in IEEE 802.11 distributed coordination function （DCF）. In the noisy channel, when signal-to-noise ratio （SNR） is low, applying this mechanism in DCF greatly improves the throughput and lowers the channel idle time. This paper presents an analytical model for the performance study of IEEE 802.11 MB-DCF for nonsaturated heterogeneous traffic in the presence of transmission errors. First, we introduce the MB-DCF and compare its performance to IEEE 802.11 DCF with binary exponential backoff （BEB）. The IEEE 802.11 DCF with BEB mechanism suffers from more channel idle time under low SNR. The MB-DCF ensures high throughput and low packet delay by reducing the channel idle time under the low traffic in the network. However, to the best of the authors＇ knowledge, there are no previous works that enhance the performance of the DCF under imperfect wireless channel. We show through analysis that the proposed mechanism greatly outperforms the original IEEE 802.11 DCF in the imperfect channel condition. The effectiveness of physical and link layer parameters on throughput performance is explored. We also present a throughput investigation of the heterogeneous traffic for different radio conditions.展开更多
There has been growing research interest in the use of molybdenum disulfide in the fields of optoelectronics and energy harvesting devices, by virtue of its indirect-to-direct band gap tunability. However, obtaining l...There has been growing research interest in the use of molybdenum disulfide in the fields of optoelectronics and energy harvesting devices, by virtue of its indirect-to-direct band gap tunability. However, obtaining large area thin films of MoS2 for future device applications still remains a challenge. In the present study, the amounts of the precursors （S and MOO3） were varied systematically in order to optimize the growth of highly crystalline and large area MoS2 layers by the chemical vapor deposition method. Careful control of the amounts of precursors was found to the key factor in the synthesis of large area highly crystalline flakes. The thickness of the layers was confirmed by Raman spectroscopy and atomic force microscopy. The optical properties and chemical composition were studied by photoluminescence （PL） and X-ray photoelectron spectroscopy. The emergence of strong direct excitonic emissions at 1.82 eV （A-exciton, with a normalized PL intensity of -55 × 10^3） and 1.98 eV （B-exciton, with a normalized PL intensity of -5 × 10^3） of the sample at room temperature clearly indicates the high luminescence quantum efficiency. The mobility of the films was found to be 0.09 cm^2/（V.s） at room temperature. This study provides a method for the controlled synthesis of high-quality two-dimensional （2D） transition metal dichalcogenide materials, useful for applications in nanodevices, optoelectronics and solar energv conversion.展开更多
Objective:To study the antibacterial activity of the leaf methanol extracts of five different species of Vitex namely,Vilex altissima(V.allissima),Vitex diversifolia(V.diversifolia),Vitex negundo(V.negundo),Vitex pedu...Objective:To study the antibacterial activity of the leaf methanol extracts of five different species of Vitex namely,Vilex altissima(V.allissima),Vitex diversifolia(V.diversifolia),Vitex negundo(V.negundo),Vitex peduncularis(V.peduncularis) and Vitex trifolia(V.trifolia). Methods:Antibacterial assay was carried out by using disc diffusion method,determination of minimum inhibitory concentrations(MIC) and minimum bactericidal concentrations(MBC) against five strains of Gram-positive and seven strains of Gram-negative human pathogenic bacterial strains.Results:The results of antibacterial activity of Vitex species showed that the extracts possessed a broad spectrum of antibacterial activity.The V.peduncularis possessed the highest activity against all the microorganisms screened.It produced a zone of inhibition ranged between (11.000±0.577) and(22.670±0.667) mm and the MIC values were from 62.5 to I 000.0μg/mL and the MBC values were from 125.0 to 2 000.0μg/mL.Conclusions:Based on the present study, V.peduncularis is recommended for the isolation of antibacterial molecule responsible for the activity against the tested human pathogenic bacterial strains.展开更多
Objective:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata(R.imbricata) in HT-29 human colon cancer cell line.Methods:The successively extracted rhizome ...Objective:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata(R.imbricata) in HT-29 human colon cancer cell line.Methods:The successively extracted rhizome of R.imbricata using various solvents was analyzed for their total phenolics,tannins and flavonoid contents.In vitro antioxidant activity was evaluated by employing different assays,including DPPH,ABTS radical scavenging assays,FRAP, phosphomolybdenum reduction assay,superoxide anion,hydroxyl radical scavenging activities and metal chelating ability.Results:Acetone and methanol extracts recorded higher phenolic content and showed comparable antioxidant activity with standard reference.Additionally,they also inhibited the proliferation of HT-29 cells upon treatment at higher concentration(200μg/mL) (acetone and methanol,84%and 84%.respectively).On examination acetone extract exhibited antiproliferative activity in a concentration dependent manner whereas,methanol extract showed both dose dependent and lime dependent inhibitory activity.Conclusions:The results obtained justify the traditional usage of R.imbricata from their promising antioxidant activity.展开更多
The importance of sorting proteins and wall materials to their destination is critical for plant growth and development, though the machinery orchestrating membrane trafficking is poorly understood. Transporters that ...The importance of sorting proteins and wall materials to their destination is critical for plant growth and development, though the machinery orchestrating membrane trafficking is poorly understood. Transporters that alter the environment across endomembrane compartments are thought to be important players. Using Escherichia coli and yeast, we previously showed that several Arabidopsis Cation/H＋ eXchanger （AtCHX） members were K＋ transporters with a role in pH homeostasis, though their subcellular location and biological roles in plants are unclear. Co-expression of markers with CHX16, CHX17, CHX18, or CHX19 tagged with a fluorescent protein indicated these transporters associated with plasma membrane （PM） and post-Golgi compartments. Under its native promoter, AtCHX17（l_820）-GFP localized to prevacuolar compartment （PVC） and to PM in roots. Brefeldin A diminished AtCHX17- GFP fluorescence at PM, whereas wortmannin caused formation of GFP-labeled ring-like structures, suggesting AtCHX17 trafficked among PVC, vacuole and PM. AtCHX17（1-472） lacking its carboxylic tail did not associate with PVC or PM in plant cells. Single chx17 mutant or higher-order mutants showed normal root growth and vegetative devel- opment. However, quadruple （chx16chx17chxlSchx19） mutants were reduced in frequency and produced 50%-70% fewer seeds, indicating overlapping roles of several AtCHX17-related transporters in reproduction and/or seed devel- opment. Together, our results suggest that successful reproduction and seed development depend on the ability to regulate cation and pH homeostasis by AtCHX17-1ike transporters on membranes that traffic in the endocytic and/or secretory pathways.展开更多
In this paper, delay-dependent robust stabilization and H∞ control for uncertain stochastic Takagi-Sugeno （T-S） fuzzy systems with discrete interval and distributed time-varying delays are discussed. The purpose of...In this paper, delay-dependent robust stabilization and H∞ control for uncertain stochastic Takagi-Sugeno （T-S） fuzzy systems with discrete interval and distributed time-varying delays are discussed. The purpose of the robust stochastic stabilization problem is to design a memoryless state feedback controller such that the closed-loop system is mean-square asymptotically stable for all admissible uncertainties. In the robust H∞ control problem, in addition to the mean-square asymptotic stability requirement, a prescribed H∞ performance is required to be achieved. Sufficient conditions for the solvability of these problems are proposed in terms of a set of linear matrix inequalities （LMIs） and solving these LMIs, a desired controller can be obtained. Finally, two numerical examples are given to illustrate the effectiveness and less conservativeness of our results over the existing ones.展开更多
Industrial Internet of Things(IIoT)offers efficient communication among business partners and customers.With an enlargement of IoT tools connected through the internet,the ability of web traffic gets increased.Due to ...Industrial Internet of Things(IIoT)offers efficient communication among business partners and customers.With an enlargement of IoT tools connected through the internet,the ability of web traffic gets increased.Due to the raise in the size of network traffic,discovery of attacks in IIoT and malicious traffic in the early stages is a very demanding issues.A novel technique called Maximum Posterior Dichotomous Quadratic Discriminant Jaccardized Rocchio Emphasis Boost Classification(MPDQDJREBC)is introduced for accurate attack detection wi th minimum time consumption in IIoT.The proposed MPDQDJREBC technique includes feature selection and categorization.First,the network traffic features are collected from the dataset.Then applying the Maximum Posterior Dichotomous Quadratic Discriminant analysis to find the significant features for accurate classification and minimize the time consumption.After the significant features selection,classification is performed using the Jaccardized Rocchio Emphasis Boost technique.Jaccardized Rocchio Emphasis Boost Classification technique combines the weak learner result into strong output.Jaccardized Rocchio classification technique is considered as the weak learners to identify the normal and attack.Thus,proposed MPDQDJREBC technique gives strong classification results through lessening the quadratic error.This assists for proposed MPDQDJREBC technique to get better the accuracy for attack detection with reduced time usage.Experimental assessment is carried out with UNSW_NB15 Dataset using different factors such as accuracy,precision,recall,F-measure and attack detection time.The observed results exhibit the MPDQDJREBC technique provides higher accuracy and lesser time consumption than the conventional techniques.展开更多
Objective:To investigate the impact of COVID-19 on the mortality trends and patterns in a tertiary teaching hospital in Raipur,Chhattisgarh,India.Methods:A hospital-based retrospective study was conducted to analyze t...Objective:To investigate the impact of COVID-19 on the mortality trends and patterns in a tertiary teaching hospital in Raipur,Chhattisgarh,India.Methods:A hospital-based retrospective study was conducted to analyze the case reports of all deaths from January 2016 to December 2021 in a tertiary teaching hospital in Raipur,Chhattisgarh,India.The socio-demographic profile and the pattern of causes of death were recorded.The deaths were classified according to the 10th revision of the International Classification of Diseases.Results:A total of 6128 deaths were registered from 2016 to 2021 and the maximum death number was observed in 2021(38.2%)followed by 2020(32.5%)when the country was hit by the COVID-19 pandemic.The highest number of deaths was observed in the age group of 51 to 65 years i.e.,31.1%of the total deaths.In all five years,male deaths were more than female deaths,and more than 50%occurred within three days of hospitalization during the study period.Conclusions:Even though there is a rise in the death rate due to the sudden hit of COVID-19,the burden of both communicable and non-communicable diseases remains gradually increasing over the five years.There is a need for health awareness in the community about changing lifestyles and their harmful effects on health.展开更多
Parkinson’s disease (PD) is a debilitating neurological disorder that affects <span>the aged population globally. This study aimed to explore how oral- and in</span>traperitoneal-rotenone-induced PD alter...Parkinson’s disease (PD) is a debilitating neurological disorder that affects <span>the aged population globally. This study aimed to explore how oral- and in</span>traperitoneal-rotenone-induced PD alters brain urea levels, histopathology, and key Parkinsonism<span>-related genes in the striatum. Hematoxylin and eosin staining was performed for histopathology assessment and real-time polymerase chain reaction was performed for gene expression. Rotenone 3 mg/kg body weight (Rot-3-ip) for 21 days and rotenone 50 mg/kg body weight (Rot-50-po) for 28 days significantly (p < 0.05) altered alpha-synuclein and tyrosine hydroxylase protein expression and <i>Snca</i>, <i>Becn</i>1 and <i>Prkaa</i>1 gene expression in the striatum. Lewy bodies were visible in both Rot-3-ip and Rot-50-po rat brains. There were </span><span>contrasting features in brain and liver histopathology between the oral and</span><span> intraperitoneal rotenone treatment groups. However, there was no significant (p < 0.05) difference in the brain urea levels between intraperitoneal and oral rotenone treatment groups. The propagation of PD through oral and intraperitoneal rotenone</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:"">can have different impacts on the pathological sequence of events based on the molecular approach.展开更多
Health-care professionals acquire negative and discriminatory attitudes toward mental health.Psychiatric nursing is considered a less valuable field and they choose to work in a psychiatric setting only when there are...Health-care professionals acquire negative and discriminatory attitudes toward mental health.Psychiatric nursing is considered a less valuable field and they choose to work in a psychiatric setting only when there are no other employment opportunities.The global burden of disease study(2017)shows that 197.3 million people had mental disorders in India and one in seven Indians were affected by mental disorders.Extending the theory and clinical posting hours in psychiatry helps to promote a sense of preparedness and reducing the negative stereotypes can contribute to develop an interest in a future career in psychiatric nursing.Mental health is the foundation for the well-being and effective functioning of an individual.They play a plethora of roles such as case manager(clinical nurse),mentor,lecturer,researcher,policymaker,counselor,and clinical psychologist.Hence,it is the right time to choose this right field,which has a lot of opportunities.展开更多
Alzheimer’s disease(AD)is a neurodegenerative disorder characterized by the predominant impairment of neurons in the hippocampus and the formation of amyloid plaques,hyperphosphorylated tau protein,and neurofibrillar...Alzheimer’s disease(AD)is a neurodegenerative disorder characterized by the predominant impairment of neurons in the hippocampus and the formation of amyloid plaques,hyperphosphorylated tau protein,and neurofibrillary tangles in the brain.The overexpression of amyloid-βprecursor protein(APP)in an AD brain results in the binding of APP intracellular domain(AICD)to Fe65 protein via the C-terminal Fe65-PTB2 interaction,which then triggers the secretion of amyloid-βand the consequent pathogenesis of AD.Apparently,targeting the interaction between APP and Fe65 can offer a promising therapeutic approach for AD.Recently,exosome,a type of extracellular vesicle with diameter around 30–200 nm,has gained much attention as a potential delivery tool for brain diseases,including AD,due to their ability to cross the blood–brain barrier,their efficient uptake by autologous cells,and their ability to be surface-modified with target-specific receptor ligands.Here,the engineering of hippocampus neuron cell-derived exosomes to overexpress Fe65,enabled the development of a novel exosome-based targeted drug delivery system,which carried Corynoxine-B(Cory-B,an autophagy inducer)to the APP overexpressed-neuron cells in the brain of AD mice.The Fe65-engineered HT22 hippocampus neuron cell-derived exosomes(Fe65-EXO)loaded with Cory-B(Fe65-EXO-Cory-B)hijacked the signaling and blocked the natural interaction between Fe65 and APP,enabling APP-targeted delivery of Cory-B.Notably,Fe65-EXO-Cory-B induced autophagy in APP-expressing neuronal cells,leading to amelioration of the cognitive decline and pathogenesis in AD mice,demonstrating the potential of Fe65-EXO-Cory-B as an effective therapeutic intervention for AD.展开更多
Leprosy with multidrug therapy and treatment in the early stages can prevent disability,but it still represents a significant health problem,mainly in vulnerable populations[1].However,if left untreated,leprosy can ca...Leprosy with multidrug therapy and treatment in the early stages can prevent disability,but it still represents a significant health problem,mainly in vulnerable populations[1].However,if left untreated,leprosy can cause progressive and permanent damage to the skin,nerves,limbs,and eyes.With the constant efforts of the National Leprosy Elimination Programme in India,the implementation of multidrug therapy has lowered the prevalence rate to 0.66/10000 in 2016[1].展开更多
AIM: To prospectively analyse the clinical, biochemical and radiological characteristics of the mass lesions arising in a background of chronic calcific pancreatitis (CCP). METHODS: Eighty three patients, who presente...AIM: To prospectively analyse the clinical, biochemical and radiological characteristics of the mass lesions arising in a background of chronic calcific pancreatitis (CCP). METHODS: Eighty three patients, who presented with chronic pancreatitis (CP) and a mass lesion in the head of pancreas between February 2005 and December 2011, were included in the study. Patients who were identified to have malignancy underwent Whipple' s procedure and patients whose investigations were suggestive of a benign lesion underwent Frey's procedure. Student t-test was used to compare the mean values of imaging findings [common bile duct (CBD), main pancreatic duct (MPD) size] and laboratory data [Serum bilirubin, carbohydrate antigen 19-9 (CA 19-9)] between the groups. Receiver operating characteristic curve (ROC curve) analysis was done to calculate the cutoff valves of serum bilirubin, CA 19-9, MPD and CBD size. The sensitivity, specificity, positive predictive valve (PPV) and negative predictive value (NPV) were calculated using these cut off points. Multivariate analysis was performed using logistic regression model. RESULTS: The study included 56 men (67.5%) and 27 women (32.5%). Sixty (72.3%) patients had tropical calcific pancreatitis and 23 (27.7%) had alcohol related CCP. Histologically, it was confirmed that 55 (66.3%) of the 83 patients had an inflammatory head mass and 28 (33.7%) had a malignant head mass. The mean age of individuals with benign inflammatory mass and those with malignant mass was 38.4 years and 45 years respectively. Significant clinical features that predicted a malignant head mass in CP were presence of a head mass in CCP of tropics, old age, jaundice, sudden worsening abdominal pain, gastric outlet obstruction and significant weight loss (P ≤ 0.05). The ROC curve analysis showed a cut off value of 5.8 mg/dL for serum bilirubin, 127 U/mL for CA 19-9, 11.5 mm for MPD size and 14.5 mm for CBD size. CONCLUSION: Elevated Serum bilirubin and CA 19-9, and dilated MPD and CBD were useful in predicting mali展开更多
Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in biopreservation.Methods:Bacillus cereus(B.cereus)was isolat...Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in biopreservation.Methods:Bacillus cereus(B.cereus)was isolated and assessed for its baceteriocinogenic activity.Bacteriocin was produced and purified by ammonium sulphate precipitation,dialysis and gel filtration chromatography.Purified bacteriocin was used to clieck its antimicrobial activity against food borne bacteria.Effect and stability of bacteripcin was determined with the respect to temperature,pH,enzymes,organic solvents and chemicals.Bacteriocin was also subjected to SDS PAGE analysis to determine its molecular weight.In addition,functional groups exist in the bacteriocin was determined by FTIR analysis.Results:B.cereus was identified by 16S rRNA sequence analysis.Bacteriocin showed increased activity against all the bacteria used and its activity unit was found to be 51,200 AU/mL.It was stable to high temperature(100℃)and wide range of pH(3-10),sensitive to proteolytic enzymes and resistant to nonprotcolytic enzymes.It was low molecular weight(3.5-6 kDa)protein and FTIR study revealed the presence of amide group and NH stretching,Conclusions:Bacteriocin produced in this study possesses the highest antimicrobial activity against both gram positive and gram negative bacteria thereby it has immense application as biopreservative agent.FTIR proved its peptide nature.展开更多
Background:Computer simulation studies complement in vitro experiments and provide avenue to understand allosteric regulation in the absence of other molecular viewing techniques.Molecular dynamics captures internal m...Background:Computer simulation studies complement in vitro experiments and provide avenue to understand allosteric regulation in the absence of other molecular viewing techniques.Molecular dynamics captures internal motion within the protein and enables tracing the communication path between a catalytic site and a distal allosteric site.In this article,we have identified the communication pathway between the viral protein genome linked(VPg)binding region and catalytic active site in nuclear inclusion protein-a protease(NIa-Pro).Methods:Molecular dynamics followed by in silico analyses have been used to map the allosteric pathway.Results:This study delineates the residue interaction network involved in allosteric regulation of NIa-Pro activity by VPg.Simulation studies indicate that point mutations in the VPg interaction interface of NIa-Pro lead to disruption in these networks and change the orientation of catalytic residues.His142Ala and His167Ala mutations do not show a substantial change in the overall protease structure,but rather in the residue interaction network and catalytic site geometry.Conclusion:Our mutagenic study delineates the allosteric pathway and facilitates the understanding of the modulation of NIa-Pro activity on a molecular level in the absence of the structure of its complex with the known regulator VPg.Additionally,our in silico analysis explains the molecular concepts and highlights the dynamics behind the previously reported wet lab study findings.展开更多
Human Action Recognition(HAR)attempts to recognize the human action from images and videos.The major challenge in HAR is the design of an action descriptor that makes the HAR system robust for different environments.A...Human Action Recognition(HAR)attempts to recognize the human action from images and videos.The major challenge in HAR is the design of an action descriptor that makes the HAR system robust for different environments.A novel action descriptor is proposed in this study,based on two independent spatial and spectral filters.The proposed descriptor uses a Difference of Gaussian(DoG)filter to extract scale-invariant features and a Difference of Wavelet(DoW)filter to extract spectral information.To create a composite feature vector for a particular test action picture,the Discriminant of Guassian(DoG)and Difference of Wavelet(DoW)features are combined.Linear Discriminant Analysis(LDA),a widely used dimensionality reduction technique,is also used to eliminate duplicate data.Finally,a closest neighbor method is used to classify the dataset.Weizmann and UCF 11 datasets were used to run extensive simulations of the suggested strategy,and the accuracy assessed after the simulations were run on Weizmann datasets for five-fold cross validation is shown to perform well.The average accuracy of DoG+DoW is observed as 83.6635%while the average accuracy of Discrinanat of Guassian(DoG)and Difference of Wavelet(DoW)is observed as 80.2312%and 77.4215%,respectively.The average accuracy measured after the simulation of proposed methods over UCF 11 action dataset for five-fold cross validation DoG+DoW is observed as 62.5231%while the average accuracy of Difference of Guassian(DoG)and Difference of Wavelet(DoW)is observed as 60.3214%and 58.1247%,respectively.From the above accuracy observations,the accuracy of Weizmann is high compared to the accuracy of UCF 11,hence verifying the effectiveness in the improvisation of recognition accuracy.展开更多
The tremendous growth in the field of modern communication and network systems places demands on the security. As the network complexity grows, the need for the automated detection and timely alert is required to dete...The tremendous growth in the field of modern communication and network systems places demands on the security. As the network complexity grows, the need for the automated detection and timely alert is required to detect the abnormal activities in the network. To diagnose the system against the malicious signatures, a high speed Network Intrusion Detection System is required against the attacks. In the network security applications, Bloom Filters are the key building block. The packets from the high speed link can be easily processed by Bloom Filter using state- of-art hardware based systems. As Bloom Filter and its variant Counting Bloom Filter suffer from False Positive Rate, Multi Hash Counting Bloom Filter architecture is proposed. The proposed work, constitute parallel signature detection improves the False Positive Rate, but the throughput and hardware complexity suffer. To resolve this, a Multi-Level Ranking Scheme is introduced which deduces the 13% - 16% of the power and increases the throughput to 23% - 30%. This work is best suited for signature detection in high speed network.展开更多
文摘In this paper, we propose a mechanism named modified backoff （MB） mechanism to decrease the channel idle time in IEEE 802.11 distributed coordination function （DCF）. In the noisy channel, when signal-to-noise ratio （SNR） is low, applying this mechanism in DCF greatly improves the throughput and lowers the channel idle time. This paper presents an analytical model for the performance study of IEEE 802.11 MB-DCF for nonsaturated heterogeneous traffic in the presence of transmission errors. First, we introduce the MB-DCF and compare its performance to IEEE 802.11 DCF with binary exponential backoff （BEB）. The IEEE 802.11 DCF with BEB mechanism suffers from more channel idle time under low SNR. The MB-DCF ensures high throughput and low packet delay by reducing the channel idle time under the low traffic in the network. However, to the best of the authors＇ knowledge, there are no previous works that enhance the performance of the DCF under imperfect wireless channel. We show through analysis that the proposed mechanism greatly outperforms the original IEEE 802.11 DCF in the imperfect channel condition. The effectiveness of physical and link layer parameters on throughput performance is explored. We also present a throughput investigation of the heterogeneous traffic for different radio conditions.
文摘There has been growing research interest in the use of molybdenum disulfide in the fields of optoelectronics and energy harvesting devices, by virtue of its indirect-to-direct band gap tunability. However, obtaining large area thin films of MoS2 for future device applications still remains a challenge. In the present study, the amounts of the precursors （S and MOO3） were varied systematically in order to optimize the growth of highly crystalline and large area MoS2 layers by the chemical vapor deposition method. Careful control of the amounts of precursors was found to the key factor in the synthesis of large area highly crystalline flakes. The thickness of the layers was confirmed by Raman spectroscopy and atomic force microscopy. The optical properties and chemical composition were studied by photoluminescence （PL） and X-ray photoelectron spectroscopy. The emergence of strong direct excitonic emissions at 1.82 eV （A-exciton, with a normalized PL intensity of -55 × 10^3） and 1.98 eV （B-exciton, with a normalized PL intensity of -5 × 10^3） of the sample at room temperature clearly indicates the high luminescence quantum efficiency. The mobility of the films was found to be 0.09 cm^2/（V.s） at room temperature. This study provides a method for the controlled synthesis of high-quality two-dimensional （2D） transition metal dichalcogenide materials, useful for applications in nanodevices, optoelectronics and solar energv conversion.
文摘Objective:To study the antibacterial activity of the leaf methanol extracts of five different species of Vitex namely,Vilex altissima(V.allissima),Vitex diversifolia(V.diversifolia),Vitex negundo(V.negundo),Vitex peduncularis(V.peduncularis) and Vitex trifolia(V.trifolia). Methods:Antibacterial assay was carried out by using disc diffusion method,determination of minimum inhibitory concentrations(MIC) and minimum bactericidal concentrations(MBC) against five strains of Gram-positive and seven strains of Gram-negative human pathogenic bacterial strains.Results:The results of antibacterial activity of Vitex species showed that the extracts possessed a broad spectrum of antibacterial activity.The V.peduncularis possessed the highest activity against all the microorganisms screened.It produced a zone of inhibition ranged between (11.000±0.577) and(22.670±0.667) mm and the MIC values were from 62.5 to I 000.0μg/mL and the MBC values were from 125.0 to 2 000.0μg/mL.Conclusions:Based on the present study, V.peduncularis is recommended for the isolation of antibacterial molecule responsible for the activity against the tested human pathogenic bacterial strains.
基金supported by the DIHAR,Defense Research Development Organization Indiathe Food Security Center,University of Hohenheim,Stuttgart,Germany for awarding the Sandwich Scholarship
文摘Objective:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata(R.imbricata) in HT-29 human colon cancer cell line.Methods:The successively extracted rhizome of R.imbricata using various solvents was analyzed for their total phenolics,tannins and flavonoid contents.In vitro antioxidant activity was evaluated by employing different assays,including DPPH,ABTS radical scavenging assays,FRAP, phosphomolybdenum reduction assay,superoxide anion,hydroxyl radical scavenging activities and metal chelating ability.Results:Acetone and methanol extracts recorded higher phenolic content and showed comparable antioxidant activity with standard reference.Additionally,they also inhibited the proliferation of HT-29 cells upon treatment at higher concentration(200μg/mL) (acetone and methanol,84%and 84%.respectively).On examination acetone extract exhibited antiproliferative activity in a concentration dependent manner whereas,methanol extract showed both dose dependent and lime dependent inhibitory activity.Conclusions:The results obtained justify the traditional usage of R.imbricata from their promising antioxidant activity.
基金This work was supported in part by National Science Foundation，by the US Department of Energy,Division of Chemical Sciences,Geosciences and Biosciences,Office of Basic Energy Sciences (BES DEFG0207ER15883) to H.S，a Royal Thai Government Graduate Fellowship to S.C，by research grants from Academia Sinica，the National Science and Technology Program for Agricultural Biotechnology，the National Science Council (99-2321-B-001-036-MY3) to G.Y.J
文摘The importance of sorting proteins and wall materials to their destination is critical for plant growth and development, though the machinery orchestrating membrane trafficking is poorly understood. Transporters that alter the environment across endomembrane compartments are thought to be important players. Using Escherichia coli and yeast, we previously showed that several Arabidopsis Cation/H＋ eXchanger （AtCHX） members were K＋ transporters with a role in pH homeostasis, though their subcellular location and biological roles in plants are unclear. Co-expression of markers with CHX16, CHX17, CHX18, or CHX19 tagged with a fluorescent protein indicated these transporters associated with plasma membrane （PM） and post-Golgi compartments. Under its native promoter, AtCHX17（l_820）-GFP localized to prevacuolar compartment （PVC） and to PM in roots. Brefeldin A diminished AtCHX17- GFP fluorescence at PM, whereas wortmannin caused formation of GFP-labeled ring-like structures, suggesting AtCHX17 trafficked among PVC, vacuole and PM. AtCHX17（1-472） lacking its carboxylic tail did not associate with PVC or PM in plant cells. Single chx17 mutant or higher-order mutants showed normal root growth and vegetative devel- opment. However, quadruple （chx16chx17chxlSchx19） mutants were reduced in frequency and produced 50%-70% fewer seeds, indicating overlapping roles of several AtCHX17-related transporters in reproduction and/or seed devel- opment. Together, our results suggest that successful reproduction and seed development depend on the ability to regulate cation and pH homeostasis by AtCHX17-1ike transporters on membranes that traffic in the endocytic and/or secretory pathways.
文摘In this paper, delay-dependent robust stabilization and H∞ control for uncertain stochastic Takagi-Sugeno （T-S） fuzzy systems with discrete interval and distributed time-varying delays are discussed. The purpose of the robust stochastic stabilization problem is to design a memoryless state feedback controller such that the closed-loop system is mean-square asymptotically stable for all admissible uncertainties. In the robust H∞ control problem, in addition to the mean-square asymptotic stability requirement, a prescribed H∞ performance is required to be achieved. Sufficient conditions for the solvability of these problems are proposed in terms of a set of linear matrix inequalities （LMIs） and solving these LMIs, a desired controller can be obtained. Finally, two numerical examples are given to illustrate the effectiveness and less conservativeness of our results over the existing ones.
文摘Industrial Internet of Things(IIoT)offers efficient communication among business partners and customers.With an enlargement of IoT tools connected through the internet,the ability of web traffic gets increased.Due to the raise in the size of network traffic,discovery of attacks in IIoT and malicious traffic in the early stages is a very demanding issues.A novel technique called Maximum Posterior Dichotomous Quadratic Discriminant Jaccardized Rocchio Emphasis Boost Classification(MPDQDJREBC)is introduced for accurate attack detection wi th minimum time consumption in IIoT.The proposed MPDQDJREBC technique includes feature selection and categorization.First,the network traffic features are collected from the dataset.Then applying the Maximum Posterior Dichotomous Quadratic Discriminant analysis to find the significant features for accurate classification and minimize the time consumption.After the significant features selection,classification is performed using the Jaccardized Rocchio Emphasis Boost technique.Jaccardized Rocchio Emphasis Boost Classification technique combines the weak learner result into strong output.Jaccardized Rocchio classification technique is considered as the weak learners to identify the normal and attack.Thus,proposed MPDQDJREBC technique gives strong classification results through lessening the quadratic error.This assists for proposed MPDQDJREBC technique to get better the accuracy for attack detection with reduced time usage.Experimental assessment is carried out with UNSW_NB15 Dataset using different factors such as accuracy,precision,recall,F-measure and attack detection time.The observed results exhibit the MPDQDJREBC technique provides higher accuracy and lesser time consumption than the conventional techniques.
文摘Objective:To investigate the impact of COVID-19 on the mortality trends and patterns in a tertiary teaching hospital in Raipur,Chhattisgarh,India.Methods:A hospital-based retrospective study was conducted to analyze the case reports of all deaths from January 2016 to December 2021 in a tertiary teaching hospital in Raipur,Chhattisgarh,India.The socio-demographic profile and the pattern of causes of death were recorded.The deaths were classified according to the 10th revision of the International Classification of Diseases.Results:A total of 6128 deaths were registered from 2016 to 2021 and the maximum death number was observed in 2021(38.2%)followed by 2020(32.5%)when the country was hit by the COVID-19 pandemic.The highest number of deaths was observed in the age group of 51 to 65 years i.e.,31.1%of the total deaths.In all five years,male deaths were more than female deaths,and more than 50%occurred within three days of hospitalization during the study period.Conclusions:Even though there is a rise in the death rate due to the sudden hit of COVID-19,the burden of both communicable and non-communicable diseases remains gradually increasing over the five years.There is a need for health awareness in the community about changing lifestyles and their harmful effects on health.
文摘Parkinson’s disease (PD) is a debilitating neurological disorder that affects <span>the aged population globally. This study aimed to explore how oral- and in</span>traperitoneal-rotenone-induced PD alters brain urea levels, histopathology, and key Parkinsonism<span>-related genes in the striatum. Hematoxylin and eosin staining was performed for histopathology assessment and real-time polymerase chain reaction was performed for gene expression. Rotenone 3 mg/kg body weight (Rot-3-ip) for 21 days and rotenone 50 mg/kg body weight (Rot-50-po) for 28 days significantly (p < 0.05) altered alpha-synuclein and tyrosine hydroxylase protein expression and <i>Snca</i>, <i>Becn</i>1 and <i>Prkaa</i>1 gene expression in the striatum. Lewy bodies were visible in both Rot-3-ip and Rot-50-po rat brains. There were </span><span>contrasting features in brain and liver histopathology between the oral and</span><span> intraperitoneal rotenone treatment groups. However, there was no significant (p < 0.05) difference in the brain urea levels between intraperitoneal and oral rotenone treatment groups. The propagation of PD through oral and intraperitoneal rotenone</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:"">can have different impacts on the pathological sequence of events based on the molecular approach.
文摘Health-care professionals acquire negative and discriminatory attitudes toward mental health.Psychiatric nursing is considered a less valuable field and they choose to work in a psychiatric setting only when there are no other employment opportunities.The global burden of disease study(2017)shows that 197.3 million people had mental disorders in India and one in seven Indians were affected by mental disorders.Extending the theory and clinical posting hours in psychiatry helps to promote a sense of preparedness and reducing the negative stereotypes can contribute to develop an interest in a future career in psychiatric nursing.Mental health is the foundation for the well-being and effective functioning of an individual.They play a plethora of roles such as case manager(clinical nurse),mentor,lecturer,researcher,policymaker,counselor,and clinical psychologist.Hence,it is the right time to choose this right field,which has a lot of opportunities.
基金the grants of Health and Medical Research Fund HMRF/17182541(ML)Health and Medical Research Fund HMRF/17182551(AlI)+10 种基金Matching Proof-of-Concept Fund(MPCF)HKBU-MPCF-003-2022-23(AI)Health and Medical Research Fund HMRF/09203776(ML)Research Grants Council of Hong Kong,General Research Fund GRF/2100618(ML)Research Grants Council of Hong Kong,General Research Fund GRF/12101022(ML)Research Grants Council of Hong Kong,Collaborative Research Fund C2011-21GF(ML)Hong Kong Baptist University Grant HKBU/RC-IRCs/17-18/03(ML)Hong Kong Baptist University Grant IRCMS/19-20/H02(ML,Al)U.S.National Institute of Health,NCI R00 CA226353-01A1(HJC)U.S.National Institute of Health,NCI K99 CA226353-01A1(HJC)Cancer Research Foundation Young Investigator Award(HJC)LCRF pilot grant(HJC).
文摘Alzheimer’s disease(AD)is a neurodegenerative disorder characterized by the predominant impairment of neurons in the hippocampus and the formation of amyloid plaques,hyperphosphorylated tau protein,and neurofibrillary tangles in the brain.The overexpression of amyloid-βprecursor protein(APP)in an AD brain results in the binding of APP intracellular domain(AICD)to Fe65 protein via the C-terminal Fe65-PTB2 interaction,which then triggers the secretion of amyloid-βand the consequent pathogenesis of AD.Apparently,targeting the interaction between APP and Fe65 can offer a promising therapeutic approach for AD.Recently,exosome,a type of extracellular vesicle with diameter around 30–200 nm,has gained much attention as a potential delivery tool for brain diseases,including AD,due to their ability to cross the blood–brain barrier,their efficient uptake by autologous cells,and their ability to be surface-modified with target-specific receptor ligands.Here,the engineering of hippocampus neuron cell-derived exosomes to overexpress Fe65,enabled the development of a novel exosome-based targeted drug delivery system,which carried Corynoxine-B(Cory-B,an autophagy inducer)to the APP overexpressed-neuron cells in the brain of AD mice.The Fe65-engineered HT22 hippocampus neuron cell-derived exosomes(Fe65-EXO)loaded with Cory-B(Fe65-EXO-Cory-B)hijacked the signaling and blocked the natural interaction between Fe65 and APP,enabling APP-targeted delivery of Cory-B.Notably,Fe65-EXO-Cory-B induced autophagy in APP-expressing neuronal cells,leading to amelioration of the cognitive decline and pathogenesis in AD mice,demonstrating the potential of Fe65-EXO-Cory-B as an effective therapeutic intervention for AD.
文摘Leprosy with multidrug therapy and treatment in the early stages can prevent disability,but it still represents a significant health problem,mainly in vulnerable populations[1].However,if left untreated,leprosy can cause progressive and permanent damage to the skin,nerves,limbs,and eyes.With the constant efforts of the National Leprosy Elimination Programme in India,the implementation of multidrug therapy has lowered the prevalence rate to 0.66/10000 in 2016[1].
文摘AIM: To prospectively analyse the clinical, biochemical and radiological characteristics of the mass lesions arising in a background of chronic calcific pancreatitis (CCP). METHODS: Eighty three patients, who presented with chronic pancreatitis (CP) and a mass lesion in the head of pancreas between February 2005 and December 2011, were included in the study. Patients who were identified to have malignancy underwent Whipple' s procedure and patients whose investigations were suggestive of a benign lesion underwent Frey's procedure. Student t-test was used to compare the mean values of imaging findings [common bile duct (CBD), main pancreatic duct (MPD) size] and laboratory data [Serum bilirubin, carbohydrate antigen 19-9 (CA 19-9)] between the groups. Receiver operating characteristic curve (ROC curve) analysis was done to calculate the cutoff valves of serum bilirubin, CA 19-9, MPD and CBD size. The sensitivity, specificity, positive predictive valve (PPV) and negative predictive value (NPV) were calculated using these cut off points. Multivariate analysis was performed using logistic regression model. RESULTS: The study included 56 men (67.5%) and 27 women (32.5%). Sixty (72.3%) patients had tropical calcific pancreatitis and 23 (27.7%) had alcohol related CCP. Histologically, it was confirmed that 55 (66.3%) of the 83 patients had an inflammatory head mass and 28 (33.7%) had a malignant head mass. The mean age of individuals with benign inflammatory mass and those with malignant mass was 38.4 years and 45 years respectively. Significant clinical features that predicted a malignant head mass in CP were presence of a head mass in CCP of tropics, old age, jaundice, sudden worsening abdominal pain, gastric outlet obstruction and significant weight loss (P ≤ 0.05). The ROC curve analysis showed a cut off value of 5.8 mg/dL for serum bilirubin, 127 U/mL for CA 19-9, 11.5 mm for MPD size and 14.5 mm for CBD size. CONCLUSION: Elevated Serum bilirubin and CA 19-9, and dilated MPD and CBD were useful in predicting mali
基金Managemtent of Vivekananda Educational Inslitulions Tiruchengode, India for their support throughout the project
文摘Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in biopreservation.Methods:Bacillus cereus(B.cereus)was isolated and assessed for its baceteriocinogenic activity.Bacteriocin was produced and purified by ammonium sulphate precipitation,dialysis and gel filtration chromatography.Purified bacteriocin was used to clieck its antimicrobial activity against food borne bacteria.Effect and stability of bacteripcin was determined with the respect to temperature,pH,enzymes,organic solvents and chemicals.Bacteriocin was also subjected to SDS PAGE analysis to determine its molecular weight.In addition,functional groups exist in the bacteriocin was determined by FTIR analysis.Results:B.cereus was identified by 16S rRNA sequence analysis.Bacteriocin showed increased activity against all the bacteria used and its activity unit was found to be 51,200 AU/mL.It was stable to high temperature(100℃)and wide range of pH(3-10),sensitive to proteolytic enzymes and resistant to nonprotcolytic enzymes.It was low molecular weight(3.5-6 kDa)protein and FTIR study revealed the presence of amide group and NH stretching,Conclusions:Bacteriocin produced in this study possesses the highest antimicrobial activity against both gram positive and gram negative bacteria thereby it has immense application as biopreservative agent.FTIR proved its peptide nature.
文摘Background:Computer simulation studies complement in vitro experiments and provide avenue to understand allosteric regulation in the absence of other molecular viewing techniques.Molecular dynamics captures internal motion within the protein and enables tracing the communication path between a catalytic site and a distal allosteric site.In this article,we have identified the communication pathway between the viral protein genome linked(VPg)binding region and catalytic active site in nuclear inclusion protein-a protease(NIa-Pro).Methods:Molecular dynamics followed by in silico analyses have been used to map the allosteric pathway.Results:This study delineates the residue interaction network involved in allosteric regulation of NIa-Pro activity by VPg.Simulation studies indicate that point mutations in the VPg interaction interface of NIa-Pro lead to disruption in these networks and change the orientation of catalytic residues.His142Ala and His167Ala mutations do not show a substantial change in the overall protease structure,but rather in the residue interaction network and catalytic site geometry.Conclusion:Our mutagenic study delineates the allosteric pathway and facilitates the understanding of the modulation of NIa-Pro activity on a molecular level in the absence of the structure of its complex with the known regulator VPg.Additionally,our in silico analysis explains the molecular concepts and highlights the dynamics behind the previously reported wet lab study findings.
文摘Human Action Recognition(HAR)attempts to recognize the human action from images and videos.The major challenge in HAR is the design of an action descriptor that makes the HAR system robust for different environments.A novel action descriptor is proposed in this study,based on two independent spatial and spectral filters.The proposed descriptor uses a Difference of Gaussian(DoG)filter to extract scale-invariant features and a Difference of Wavelet(DoW)filter to extract spectral information.To create a composite feature vector for a particular test action picture,the Discriminant of Guassian(DoG)and Difference of Wavelet(DoW)features are combined.Linear Discriminant Analysis(LDA),a widely used dimensionality reduction technique,is also used to eliminate duplicate data.Finally,a closest neighbor method is used to classify the dataset.Weizmann and UCF 11 datasets were used to run extensive simulations of the suggested strategy,and the accuracy assessed after the simulations were run on Weizmann datasets for five-fold cross validation is shown to perform well.The average accuracy of DoG+DoW is observed as 83.6635%while the average accuracy of Discrinanat of Guassian(DoG)and Difference of Wavelet(DoW)is observed as 80.2312%and 77.4215%,respectively.The average accuracy measured after the simulation of proposed methods over UCF 11 action dataset for five-fold cross validation DoG+DoW is observed as 62.5231%while the average accuracy of Difference of Guassian(DoG)and Difference of Wavelet(DoW)is observed as 60.3214%and 58.1247%,respectively.From the above accuracy observations,the accuracy of Weizmann is high compared to the accuracy of UCF 11,hence verifying the effectiveness in the improvisation of recognition accuracy.
文摘The tremendous growth in the field of modern communication and network systems places demands on the security. As the network complexity grows, the need for the automated detection and timely alert is required to detect the abnormal activities in the network. To diagnose the system against the malicious signatures, a high speed Network Intrusion Detection System is required against the attacks. In the network security applications, Bloom Filters are the key building block. The packets from the high speed link can be easily processed by Bloom Filter using state- of-art hardware based systems. As Bloom Filter and its variant Counting Bloom Filter suffer from False Positive Rate, Multi Hash Counting Bloom Filter architecture is proposed. The proposed work, constitute parallel signature detection improves the False Positive Rate, but the throughput and hardware complexity suffer. To resolve this, a Multi-Level Ranking Scheme is introduced which deduces the 13% - 16% of the power and increases the throughput to 23% - 30%. This work is best suited for signature detection in high speed network.
