BACKGROUND Early diagnosis of hepatocellular carcinoma may help to ensure that patients have a chance for long-term survival;however,currently available biomarkers lack sensitivity and specificity.AIM To characterize ...BACKGROUND Early diagnosis of hepatocellular carcinoma may help to ensure that patients have a chance for long-term survival;however,currently available biomarkers lack sensitivity and specificity.AIM To characterize the serum metabolome of hepatocellular carcinoma in order to develop a new metabolomics diagnostic model and identifying novel biomarkers for screening hepatocellular carcinoma based on the pattern recognition method.METHODS Ultra-performance liquid chromatography-mass spectroscopy was used to characterize the serum metabolome of hepatocellular carcinoma(n=30)and cirrhosis(n=29)patients,followed by sequential feature selection combined with linear discriminant analysis to process the multivariate data.RESULTS The concentrations of most metabolites,including proline,were lower in patients with hepatocellular carcinoma,whereas the hydroxypurine levels were higher in these patients.As ordinary analysis models failed to discriminate hepatocellular carcinoma from cirrhosis,pattern recognition analysis was used to establish a pattern recognition model that included hydroxypurine and proline.The leaveone-out cross-validation accuracy and area under the receiver operating characteristic curve analysis were 95.00%and 0.90[95%Confidence Interval(CI):0.81-0.99]for the training set,respectively,and 78.95%and 0.84(95%CI:0.67-1.00)for the validation set,respectively.In contrast,forα-fetoprotein,the accuracy and area under the receiver operating characteristic curve were 65.00%and 0.69(95%CI:0.52-0.86)for the training set,respectively,and 68.42%and 0.68(95%CI:0.41-0.94)for the validation set,respectively.The Z test revealed that the area under the curve of the linear discriminant analysis model was significantly higher than the area under the curve ofα-fetoprotein(P<0.05)in both the training and validation sets.CONCLUSION Hydroxypurine and proline might be novel biomarkers for hepatocellular carcinoma,and this disease could be diagnosed by the metabolomics model based on pattern recognition.展开更多
Epstein-Barr virus(EBV)and human papillomavirus(HPV)infection is the risk factors for nasopharyngeal carcinoma and cervical carcinoma,respectively.However,clinical analyses demonstrate that EBV or HPV is associated wi...Epstein-Barr virus(EBV)and human papillomavirus(HPV)infection is the risk factors for nasopharyngeal carcinoma and cervical carcinoma,respectively.However,clinical analyses demonstrate that EBV or HPV is associated with improved response of patients,although underlying mechanism remains unclear.Here,we reported that the oncoproteins of DNA viruses,such as LMP1 of EBV and E7 of HPV,inhibit PERK activity in cancer cells via the interaction of the viral oncoproteins with PERK through a conserved motif.Inhibition of PERK led to increased level of reactive oxygen species(ROS)that promoted tumor and enhanced the efficacy of chemotherapy in vivo.Consistently,disruption of viral oncoprotein-PERK interactions attenuated tumor growth and chemotherapy in both cancer cells and tumor-bearing mouse models.Our findings uncovered a paradoxical effect of DNA tumor virus oncoproteins on tumors and highlighted that targeting PERK might be an attractive strategy for the treatment of NPC and cervical carcinoma.展开更多
目的分析急危重症口腔颌面部感染患者的临床特点,为提高治疗效果提供依据。方法选取2015年1月至2016年12月首都医科大学附属北京友谊医院口腔科与重症医学科联合诊治的急危重症口腔颌面部感染患者38例,分析其感染源、病原菌培养、耐药...目的分析急危重症口腔颌面部感染患者的临床特点,为提高治疗效果提供依据。方法选取2015年1月至2016年12月首都医科大学附属北京友谊医院口腔科与重症医学科联合诊治的急危重症口腔颌面部感染患者38例,分析其感染源、病原菌培养、耐药检测、治疗结果。结果感染源主要以牙源性[21例(55.3%)]为主,病原菌培养结果以链球菌属为主,占检出菌种的77.8%,耐药检测结果万古霉素的耐药率(2.8%)最低。急性生理和慢性健康状况评分Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)<15分患者的住院天数为(12.7±4.7)天,治愈率为100%,而APACHEⅡ评分≥15分患者的住院天数为(22.3±9.9)天,治愈率为84.6%,两组比较差异有显著性(P<0.05)。结论口腔颌面外科局部手术联合重症监护病房的全身支持、抗炎,可有效治疗急危重症口腔颌面部感染,且APACHEⅡ评分可帮助判断预后。展开更多
超过12%的人类肿瘤与病毒密切相关,如Epstein-Barr virus(EBV)、high-risk human papillomaviruses(HPV)、hepatitis B virus(HBV)、hepatitis C virus(HCV)、Kaposi’s sarcoma herpesvirus(KSHV)等。传统观点认为病毒调控细胞恶性转...超过12%的人类肿瘤与病毒密切相关,如Epstein-Barr virus(EBV)、high-risk human papillomaviruses(HPV)、hepatitis B virus(HBV)、hepatitis C virus(HCV)、Kaposi’s sarcoma herpesvirus(KSHV)等。传统观点认为病毒调控细胞恶性转化及肿瘤发生主要与病毒编码蛋白相关,但随着组学和测序技术发展,人们开始认识到病毒非编码RNA在肿瘤发生发展中的重要性。这些非编码RNA不翻译为蛋白质,仅在RNA水平参与对宿主细胞和病毒自身生命活动的多方面调控。现将针对长非编码RNA、短非编码RNA及microRNA在肿瘤中的调控进行综述。展开更多
The latent membrane protein(LMP1)encoded by EBV is expressed in the majority of EBV-associated human malignancies,suggesting it is one of the major oncogenic factors in EBV-mediated carcinogenesis.In the previous stud...The latent membrane protein(LMP1)encoded by EBV is expressed in the majority of EBV-associated human malignancies,suggesting it is one of the major oncogenic factors in EBV-mediated carcinogenesis.In the previous studies we experimentally demonstrated that the DNAzymes targeting LMP1 could specifically down-regulate the expression of LMP1,leading to an increased radiosensitivity both in cells and in展开更多
Signal transducers and activators of transcription-3(STAT3),a central cytoplasmic transcription factor,is frequently overexpressed and constitutively activated during malignant transformation.The overexpression of STA...Signal transducers and activators of transcription-3(STAT3),a central cytoplasmic transcription factor,is frequently overexpressed and constitutively activated during malignant transformation.The overexpression of STAT3 in melanoma cells is often observed and is suggested to be involved in tumorigenesis and development.In this study,we designed several展开更多
基金Supported by National Natural Science Foundation of China,No.81800472 and No.81670538the Science Foundation of Hunan Health Commission,No.B2019184.
文摘BACKGROUND Early diagnosis of hepatocellular carcinoma may help to ensure that patients have a chance for long-term survival;however,currently available biomarkers lack sensitivity and specificity.AIM To characterize the serum metabolome of hepatocellular carcinoma in order to develop a new metabolomics diagnostic model and identifying novel biomarkers for screening hepatocellular carcinoma based on the pattern recognition method.METHODS Ultra-performance liquid chromatography-mass spectroscopy was used to characterize the serum metabolome of hepatocellular carcinoma(n=30)and cirrhosis(n=29)patients,followed by sequential feature selection combined with linear discriminant analysis to process the multivariate data.RESULTS The concentrations of most metabolites,including proline,were lower in patients with hepatocellular carcinoma,whereas the hydroxypurine levels were higher in these patients.As ordinary analysis models failed to discriminate hepatocellular carcinoma from cirrhosis,pattern recognition analysis was used to establish a pattern recognition model that included hydroxypurine and proline.The leaveone-out cross-validation accuracy and area under the receiver operating characteristic curve analysis were 95.00%and 0.90[95%Confidence Interval(CI):0.81-0.99]for the training set,respectively,and 78.95%and 0.84(95%CI:0.67-1.00)for the validation set,respectively.In contrast,forα-fetoprotein,the accuracy and area under the receiver operating characteristic curve were 65.00%and 0.69(95%CI:0.52-0.86)for the training set,respectively,and 68.42%and 0.68(95%CI:0.41-0.94)for the validation set,respectively.The Z test revealed that the area under the curve of the linear discriminant analysis model was significantly higher than the area under the curve ofα-fetoprotein(P<0.05)in both the training and validation sets.CONCLUSION Hydroxypurine and proline might be novel biomarkers for hepatocellular carcinoma,and this disease could be diagnosed by the metabolomics model based on pattern recognition.
基金This work was funded by the following grants and associations:National Natural Science Foundations of China(81530084 and 81702721)Hunan province natural science funds for Yong scholars(2018JJ3816).
文摘Epstein-Barr virus(EBV)and human papillomavirus(HPV)infection is the risk factors for nasopharyngeal carcinoma and cervical carcinoma,respectively.However,clinical analyses demonstrate that EBV or HPV is associated with improved response of patients,although underlying mechanism remains unclear.Here,we reported that the oncoproteins of DNA viruses,such as LMP1 of EBV and E7 of HPV,inhibit PERK activity in cancer cells via the interaction of the viral oncoproteins with PERK through a conserved motif.Inhibition of PERK led to increased level of reactive oxygen species(ROS)that promoted tumor and enhanced the efficacy of chemotherapy in vivo.Consistently,disruption of viral oncoprotein-PERK interactions attenuated tumor growth and chemotherapy in both cancer cells and tumor-bearing mouse models.Our findings uncovered a paradoxical effect of DNA tumor virus oncoproteins on tumors and highlighted that targeting PERK might be an attractive strategy for the treatment of NPC and cervical carcinoma.
文摘目的分析急危重症口腔颌面部感染患者的临床特点,为提高治疗效果提供依据。方法选取2015年1月至2016年12月首都医科大学附属北京友谊医院口腔科与重症医学科联合诊治的急危重症口腔颌面部感染患者38例,分析其感染源、病原菌培养、耐药检测、治疗结果。结果感染源主要以牙源性[21例(55.3%)]为主,病原菌培养结果以链球菌属为主,占检出菌种的77.8%,耐药检测结果万古霉素的耐药率(2.8%)最低。急性生理和慢性健康状况评分Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)<15分患者的住院天数为(12.7±4.7)天,治愈率为100%,而APACHEⅡ评分≥15分患者的住院天数为(22.3±9.9)天,治愈率为84.6%,两组比较差异有显著性(P<0.05)。结论口腔颌面外科局部手术联合重症监护病房的全身支持、抗炎,可有效治疗急危重症口腔颌面部感染,且APACHEⅡ评分可帮助判断预后。
文摘超过12%的人类肿瘤与病毒密切相关,如Epstein-Barr virus(EBV)、high-risk human papillomaviruses(HPV)、hepatitis B virus(HBV)、hepatitis C virus(HCV)、Kaposi’s sarcoma herpesvirus(KSHV)等。传统观点认为病毒调控细胞恶性转化及肿瘤发生主要与病毒编码蛋白相关,但随着组学和测序技术发展,人们开始认识到病毒非编码RNA在肿瘤发生发展中的重要性。这些非编码RNA不翻译为蛋白质,仅在RNA水平参与对宿主细胞和病毒自身生命活动的多方面调控。现将针对长非编码RNA、短非编码RNA及microRNA在肿瘤中的调控进行综述。
文摘The latent membrane protein(LMP1)encoded by EBV is expressed in the majority of EBV-associated human malignancies,suggesting it is one of the major oncogenic factors in EBV-mediated carcinogenesis.In the previous studies we experimentally demonstrated that the DNAzymes targeting LMP1 could specifically down-regulate the expression of LMP1,leading to an increased radiosensitivity both in cells and in
基金Supported by the National Natural Sciences Foundation of China(No.30873010)National High Technology R&D Program of China(No.2006AA02Z481No.2009AA02Z403)
文摘Signal transducers and activators of transcription-3(STAT3),a central cytoplasmic transcription factor,is frequently overexpressed and constitutively activated during malignant transformation.The overexpression of STAT3 in melanoma cells is often observed and is suggested to be involved in tumorigenesis and development.In this study,we designed several
