Abstract [ Objective] This study was conducted to investigate the variation degree of plant traits of Dendrobium radiated by 60 Coγ ray to determine the optimum radiation dosage. [ Method] Tissue culture seedlings of...Abstract [ Objective] This study was conducted to investigate the variation degree of plant traits of Dendrobium radiated by 60 Coγ ray to determine the optimum radiation dosage. [ Method] Tissue culture seedlings of Dendrobium sonia ＇ 166＇ treated by different doses of 60 Coγ, ray were analyzed with SRAP molecular marker technique, so as to investigate its mutagenic effect. [ Result3 Genomic DNA of Dendrobium treated with 60 Coγ ray at different doses was amplified with 12 pairs of primers screened out from 100 pairs primers, and 216 bands were obtained totally. Among them, there were 41 polymorphic bands, showing a polymorphie rate of 19.0%- Clustering analysis was performed to amplified bands of different treatments and the CK. It was found that populations of different radiation treatments mu- tated to different degrees, the 20 Gy treatment mutated to the highest degree, while the 5 Gy treatment mutated to the lowest degree. [ Conclusion] It was indicated that 20 Gy is the optimum dose for radiation mutagenesis of tissue culture seedlings of D. Sonia ＂ 166＇. This study provides fundamental basis and technical support for mutation breeding by radiation and selection of mutation progenies.展开更多
为明确甘蓝型油菜花叶性状的遗传特点,开发与花叶性状连锁的分子标记。以甘蓝型油菜品系2205(圆叶)、1423(花叶)为亲本,构建了3个世代群体:F1、BC1和F2,探讨花叶性状的遗传规律;利用分子标记技术对花叶基因进行定位。结果表明,F1植株叶...为明确甘蓝型油菜花叶性状的遗传特点,开发与花叶性状连锁的分子标记。以甘蓝型油菜品系2205(圆叶)、1423(花叶)为亲本,构建了3个世代群体:F1、BC1和F2,探讨花叶性状的遗传规律;利用分子标记技术对花叶基因进行定位。结果表明,F1植株叶形表现为花叶,BC1(F1×2205)和F2中花叶与圆叶的植株分离比分别符合1∶1和3∶1,说明甘蓝型油菜的花叶性状受1对不完全显性基因控制。利用集团分离法(BSA)筛选637对SSR引物,共筛选到了3个与花叶基因紧密连锁的SSR标记:CB10079、BNGMS114和BNGMS385。连锁分析发现,这3个连锁标记均位于花叶基因的一侧,其中BNGMS114与花叶基因的遗传距离最近,其遗传距离为2.5 c M。将这3个连锁标记的序列与白菜基因组的序列进行比对,结果发现它们与白菜A10染色体的序列共线性较好,花叶基因位于A10染色体的15.70 Mb下游区段。上述标记的获得为油菜花叶性状的分子标记辅助选择育种以及花叶基因的克隆奠定理论基础。展开更多
基金Supported by China Spark Program(2015GA800008)Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630032015023,1630032017021)
文摘Abstract [ Objective] This study was conducted to investigate the variation degree of plant traits of Dendrobium radiated by 60 Coγ ray to determine the optimum radiation dosage. [ Method] Tissue culture seedlings of Dendrobium sonia ＇ 166＇ treated by different doses of 60 Coγ, ray were analyzed with SRAP molecular marker technique, so as to investigate its mutagenic effect. [ Result3 Genomic DNA of Dendrobium treated with 60 Coγ ray at different doses was amplified with 12 pairs of primers screened out from 100 pairs primers, and 216 bands were obtained totally. Among them, there were 41 polymorphic bands, showing a polymorphie rate of 19.0%- Clustering analysis was performed to amplified bands of different treatments and the CK. It was found that populations of different radiation treatments mu- tated to different degrees, the 20 Gy treatment mutated to the highest degree, while the 5 Gy treatment mutated to the lowest degree. [ Conclusion] It was indicated that 20 Gy is the optimum dose for radiation mutagenesis of tissue culture seedlings of D. Sonia ＂ 166＇. This study provides fundamental basis and technical support for mutation breeding by radiation and selection of mutation progenies.
文摘为明确甘蓝型油菜花叶性状的遗传特点,开发与花叶性状连锁的分子标记。以甘蓝型油菜品系2205(圆叶)、1423(花叶)为亲本,构建了3个世代群体:F1、BC1和F2,探讨花叶性状的遗传规律;利用分子标记技术对花叶基因进行定位。结果表明,F1植株叶形表现为花叶,BC1(F1×2205)和F2中花叶与圆叶的植株分离比分别符合1∶1和3∶1,说明甘蓝型油菜的花叶性状受1对不完全显性基因控制。利用集团分离法(BSA)筛选637对SSR引物,共筛选到了3个与花叶基因紧密连锁的SSR标记:CB10079、BNGMS114和BNGMS385。连锁分析发现,这3个连锁标记均位于花叶基因的一侧,其中BNGMS114与花叶基因的遗传距离最近,其遗传距离为2.5 c M。将这3个连锁标记的序列与白菜基因组的序列进行比对,结果发现它们与白菜A10染色体的序列共线性较好,花叶基因位于A10染色体的15.70 Mb下游区段。上述标记的获得为油菜花叶性状的分子标记辅助选择育种以及花叶基因的克隆奠定理论基础。
