Antimicrobial resistance(AMR)is one of the top global challenges of public health concern.In 2019,it was estimated that AMR claimed around 5 million deaths worldwide[1].Pseudomonas aeruginosa,which originated in soil ...Antimicrobial resistance(AMR)is one of the top global challenges of public health concern.In 2019,it was estimated that AMR claimed around 5 million deaths worldwide[1].Pseudomonas aeruginosa,which originated in soil and water,is recognized as an opportunistic pathogen causing recalcitrant infections.Carbapenem-resistant P.aeruginosa(CRPA)is listed by the World Health Organization(WHO)as an important member of top priority“ESKAPE”pathogens.This is largely supported by the fact that in 2020,CRPA had caused28,8000 nosocomial infections with about 2500 deaths.Apart from porin mutations,the acquisition of Klebsiella pneumoniae carbapenemase(termed as KPC-2)constitutes an alternative mechanism for carbapenem resistance in P.aeruginosa,which compromises the clinical efficacy of the carbapenem as a“last resort”option.Notably,the transferability of blaKPC-2 determinants facilitates global dissemination of CRPA in the context of“one health”consisting of humans,animals,plants/crops,and environmental settings.In addition to antimicrobial stewardship,coordinated global action is necessary to mitigate AMR progression.展开更多
Objective:To investigate the membrane localization function of the CX26 protein when its 86th amino acid is Thr, Ser or Arg, and its relations to deafness. Methods:CX26-GFP protein with either Thr, Ser or Arg as the 8...Objective:To investigate the membrane localization function of the CX26 protein when its 86th amino acid is Thr, Ser or Arg, and its relations to deafness. Methods:CX26-GFP protein with either Thr, Ser or Arg as the 86th amino acid was expressed in mouse SGN cells via the GFP fusion type lenti-virus expression system. The membrane localization of the fusion protein was observed under a fluorescence microscope. Results:The mutated protein of CX26 T86S was localized to cell membrane and form gap conjunction structures, showing no difference to the wild type CX26 protein (with Thr as the 86th amino acid). However, the gap conjunction structure disappeared when the mutation was CX26 T86A. Conclusion:These results indicate that the CX26 T86R mutation may be a cause of hearing loss, but CX26 T86S as a non-pathogenic poly-morphism mutation does not affect functions of the CX26 protein. The results are in accordance with the results of clinical screening.展开更多
AIM: To investigate the special Fourier transform infraredspectroscopy (FT-IR) spectra in normal and cancerous tissuesof esophagus.METHODS: Twenty-seven pairs of normal and canceroustissues of esophagus were studied b...AIM: To investigate the special Fourier transform infraredspectroscopy (FT-IR) spectra in normal and cancerous tissuesof esophagus.METHODS: Twenty-seven pairs of normal and canceroustissues of esophagus were studied by using FT-IR and thespecial spectra characteristics were analyzed in differenttissues.RESULTS: Different spectra were found in normal andcancerous tissues. The peak at 1 550/cm was weak andwide in cancerous tissues but strong and high in normaltissues.The ratio of I1 647/I1 550 was 2.0 in normal tissuesand 2.36 in cancerous tissues (P＜0.05). The ratio of Ⅰ1 550/I 1 080 was 4.5 in normal tissues and 3.4 in canceroustissues (P＜0.01). The peak at 1453/cm was higher than at1 402/cm in normal tissue and lower than at 1 402/cm incancerous tissues.CONCLUSION: The results indicate that F-FIR may be used in clinical diagnosis.展开更多
为减少联合收获机振动对谷物流量传感器监测结果的干扰,设计了一种基于声学黑洞(ABH,acoustic black hole)原理的谷物流量传感器龙门支架减振结构,通过有限元方法分析减振结构的振动特性,分析了二维声学黑洞比例系数ε、幂函数指数m和半...为减少联合收获机振动对谷物流量传感器监测结果的干扰,设计了一种基于声学黑洞(ABH,acoustic black hole)原理的谷物流量传感器龙门支架减振结构,通过有限元方法分析减振结构的振动特性,分析了二维声学黑洞比例系数ε、幂函数指数m和半径R对其减振性能的影响规律。结果表明,声学黑洞能够显著降低龙门支架的振动,ε、m和R对声学黑洞减振性能的影响均未表现出明显线性关系,当ε=0.001 2、m=2.5、R=15 mm时声学黑洞的减振效果最好。以振动速度平方和作为优化目标,建立了多项式回归代理模型,通过遗传算法对声学黑洞比例系数ε、幂函数指数m和半径R的取值进行了优化。相比于未添加声学黑洞的原始龙门支架和初始声学黑洞方案,优化后龙门支架的振动速度平方和分别降低68.92%和2%,表明优化方案具有更佳的减振性能。提出的基于声学黑洞的减振结构和优化设计方法为农业机械被动宽频减振研究提供了理论参考。展开更多
基金This work was supported by the National Natural Science Foundation of China(32141001 and 31830001 to Y.F.)the National Science Fund for Distinguished Young Scholar(32125003 to Y.F.)the National Key Basic Research Program of China(2023YFC2307100 and 2023YFC2300021 to Y.F.).
文摘Antimicrobial resistance(AMR)is one of the top global challenges of public health concern.In 2019,it was estimated that AMR claimed around 5 million deaths worldwide[1].Pseudomonas aeruginosa,which originated in soil and water,is recognized as an opportunistic pathogen causing recalcitrant infections.Carbapenem-resistant P.aeruginosa(CRPA)is listed by the World Health Organization(WHO)as an important member of top priority“ESKAPE”pathogens.This is largely supported by the fact that in 2020,CRPA had caused28,8000 nosocomial infections with about 2500 deaths.Apart from porin mutations,the acquisition of Klebsiella pneumoniae carbapenemase(termed as KPC-2)constitutes an alternative mechanism for carbapenem resistance in P.aeruginosa,which compromises the clinical efficacy of the carbapenem as a“last resort”option.Notably,the transferability of blaKPC-2 determinants facilitates global dissemination of CRPA in the context of“one health”consisting of humans,animals,plants/crops,and environmental settings.In addition to antimicrobial stewardship,coordinated global action is necessary to mitigate AMR progression.
基金supported by grants from the National Basic Research Program of China (973 Program) (#2012CB967900)National Natural Science Foundation of China (31300624, 81470684)+3 种基金Postdoctoral Science Foundation of China (2015M571818)Six Major Categories Talent (2014-WSN043, 2011-WS-074)Innovation and Entrepreneurship Training Program for College Students in Jiangsu Province (201510313003Z, 201510313003, KYLX14-1455)Clinic Medical Special Foundation of Jiangsu province (b12014032)
文摘Objective:To investigate the membrane localization function of the CX26 protein when its 86th amino acid is Thr, Ser or Arg, and its relations to deafness. Methods:CX26-GFP protein with either Thr, Ser or Arg as the 86th amino acid was expressed in mouse SGN cells via the GFP fusion type lenti-virus expression system. The membrane localization of the fusion protein was observed under a fluorescence microscope. Results:The mutated protein of CX26 T86S was localized to cell membrane and form gap conjunction structures, showing no difference to the wild type CX26 protein (with Thr as the 86th amino acid). However, the gap conjunction structure disappeared when the mutation was CX26 T86A. Conclusion:These results indicate that the CX26 T86R mutation may be a cause of hearing loss, but CX26 T86S as a non-pathogenic poly-morphism mutation does not affect functions of the CX26 protein. The results are in accordance with the results of clinical screening.
基金the National Natural Science Foundation of China, No.39730160
文摘AIM: To investigate the special Fourier transform infraredspectroscopy (FT-IR) spectra in normal and cancerous tissuesof esophagus.METHODS: Twenty-seven pairs of normal and canceroustissues of esophagus were studied by using FT-IR and thespecial spectra characteristics were analyzed in differenttissues.RESULTS: Different spectra were found in normal andcancerous tissues. The peak at 1 550/cm was weak andwide in cancerous tissues but strong and high in normaltissues.The ratio of I1 647/I1 550 was 2.0 in normal tissuesand 2.36 in cancerous tissues (P＜0.05). The ratio of Ⅰ1 550/I 1 080 was 4.5 in normal tissues and 3.4 in canceroustissues (P＜0.01). The peak at 1453/cm was higher than at1 402/cm in normal tissue and lower than at 1 402/cm incancerous tissues.CONCLUSION: The results indicate that F-FIR may be used in clinical diagnosis.
文摘为减少联合收获机振动对谷物流量传感器监测结果的干扰,设计了一种基于声学黑洞(ABH,acoustic black hole)原理的谷物流量传感器龙门支架减振结构,通过有限元方法分析减振结构的振动特性,分析了二维声学黑洞比例系数ε、幂函数指数m和半径R对其减振性能的影响规律。结果表明,声学黑洞能够显著降低龙门支架的振动,ε、m和R对声学黑洞减振性能的影响均未表现出明显线性关系,当ε=0.001 2、m=2.5、R=15 mm时声学黑洞的减振效果最好。以振动速度平方和作为优化目标,建立了多项式回归代理模型,通过遗传算法对声学黑洞比例系数ε、幂函数指数m和半径R的取值进行了优化。相比于未添加声学黑洞的原始龙门支架和初始声学黑洞方案,优化后龙门支架的振动速度平方和分别降低68.92%和2%,表明优化方案具有更佳的减振性能。提出的基于声学黑洞的减振结构和优化设计方法为农业机械被动宽频减振研究提供了理论参考。
