This study was conducted to evaluate the effects of sporoderm-broken spores of Ganoderma lucidum(SSGL),a traditional Chinese medicinal herb,on growth performance,antioxidant ability,and immunity of broilers.Three hund...This study was conducted to evaluate the effects of sporoderm-broken spores of Ganoderma lucidum(SSGL),a traditional Chinese medicinal herb,on growth performance,antioxidant ability,and immunity of broilers.Three hundred male broilers with similar body weights(40.0±1.0 g)at 1 d of age were assigned randomly to 4 treatments.Each treatment contained 5 replicates of 15 birds per replicate.The dietary treatments were corn-soybean meal basal diet supple mented with SSGL at the concentrations of0(control),100,200 and 500 mg/kg diet.The results showed that diets supplemented with SSGL significantly increased(P<0.05)the average daily gain and decreased(P<0.05)the feed:gain(F:G)ratio of birds during the finisher period(22 to 44 d of age).Moreover,the total antioxidant capability,glutathione reductase and catalase activities in the liver and spleen were significantly higher(P<0.05)in broilers fed diets with SSGL than in broilers fed the control diet.Additionally,dietary SSGL also increased(P<0.05)the serum interleukin(IL)-2,immunoglobulin(Ig)A and IgG levels of broilers compared with the control diet.These results suggest that SSGL have ameliorative effects on growth performance,free radical-scavenging activity,antioxidant capability,and immune function of broilers.展开更多
Background: The current study was carried out to evaluate the effects of mycotoxin biodegradation agent(MBA, composed of Bacillus subtilis ANSB01 G and Devosia sp. ANSB714) on relieving zearalenone(ZEA) and deoxynival...Background: The current study was carried out to evaluate the effects of mycotoxin biodegradation agent(MBA, composed of Bacillus subtilis ANSB01 G and Devosia sp. ANSB714) on relieving zearalenone(ZEA) and deoxynivalenol(DON) toxicosis in immature gilts.Methods: A total of forty pre-pubertal female gilts(61.42 ± 1.18 kg) were randomly allocated to four diet treatments: CO(positive control); MO(negative control, ZEA 596.86 μg/kg feed and DON 796 μg/kg feed);COA(CO + 2 g MBA/kg feed); MOA(MO + 2 g MBA/kg feed). Each treatment contained 10 replicates with 1 gilt per replicate. Gilts were housed in an environmentally controlled room with the partially slatted floor.Results: During the entire experimental period of 28 d, average daily gain(ADG) and average daily feed intake(ADFI)of gilts in MO group was significantly reduced compared with those in CO group. The vulva size of gilts was significantly higher in MO group than CO group. In addition, significant increases in the plasma levels of Ig A,Ig G, IL-8, IL-10 and PRL were determined in MO group compared with that in CO group. ZEA and DON in the diet upregulated apoptotic caspase-3 in ovaries and uteri, along with down-regulated the anti-apoptotic protein Bcl-2 in ovaries. The supplementation of MBA into diets co-contaminated with ZEA and DON significantly increased ADG, decreased the vulva sizes, reduced the levels of Ig G, IL-8 and PRL in plasma, and regulated apoptosis in ovaries and uteri of gilts.Conclusions: The present results indicated that feeding diet contaminated with ZEA and DON simultaneously(596.86 μg/kg + 796 μg/kg) had detrimental effects on growth performance, plasma immune function and reproductive status of gilts. And MBA could reduce the negative impacts of these two toxins, believed as a promising feed additive for mitigating toxicosis of ZEA and DON at low levels in gilts.展开更多
This note studies the Radiolarian fossil groups since 1.2 MaBP in ODP leg 184 site 1143, the southern South China Sea (SCS). The result shows that radiolarian abundance experienced a significant variation: before 0.9 ...This note studies the Radiolarian fossil groups since 1.2 MaBP in ODP leg 184 site 1143, the southern South China Sea (SCS). The result shows that radiolarian abundance experienced a significant variation: before 0.9 MaBP it remained at the extremely low level, but increased with low extent between 0.9-0.65 Ma, which corresponded to the Mid-Pleistocene Transition stage, and it increased rapidly after 0.65 MaBP. During the whole process, the average abundance became higher and higher in each stage, and showed regularly periodic fluctuations. The obvious increase after 0.65 MaBP is inferred to result from the enhanced up-welling in this region, which was induced by the intensified monsoon circulation after the 'Mid-Pleistocene Transition'. An outstanding result of the spectral analyses is that a long oscillation of - 0.2 Ma cycle was found in the records of radiolarian abundance and complex diversity, which corresponds well to the result of other paleoceanographic indexes. This probably indicated a展开更多
According to multi- disciplinary research advances in recent years, three blocks which named Jiaoliao - Bohai block, central North China block and Ordos block are divided. The differences of the tectonic association, ...According to multi- disciplinary research advances in recent years, three blocks which named Jiaoliao - Bohai block, central North China block and Ordos block are divided. The differences of the tectonic association, types, styles and features in the space, and evolution from Archaean to Cenozoic in the time, between the former two blocks are discussed. Tectonic features and evolution in the four tectonic stages of Precambrian, Indo- China, Yanshanian and Cenozoic are emphasized especially here. All of them described above have inherit and neogenic property. The temporal-spatial change and interaction among basin assemblages in the above four stages are analyzed. Escape tectonic model is employed to interpret coeval compressive and extensional basins in the study area.展开更多
背景:帕金森病是全球第二大神经退行性疾病,其运动症状和非运动症状都严重影响患者日常生活,最近研究发现外泌体及其携带的物质参与帕金森病病理发生发展,且还介导细胞通讯可作为帕金森病的潜在生物标志物。目的:探讨外泌体介导细胞通...背景:帕金森病是全球第二大神经退行性疾病,其运动症状和非运动症状都严重影响患者日常生活,最近研究发现外泌体及其携带的物质参与帕金森病病理发生发展,且还介导细胞通讯可作为帕金森病的潜在生物标志物。目的:探讨外泌体介导细胞通讯作为帕金森病的潜在生物标志物的类型及其发生作用的机制,用以寻找新的早期诊疗靶点,以期减缓帕金森病的发病进程并为其诊疗提供新思路。方法:应用计算机检索1987年1月至2022年8月PubMed、中国知网、万方数据、维普、Web of Science、EMBASE、Cochrane、EBSCO和SinoMed数据库收录的相关文献,最终纳入76篇英文文献和2篇中文文献进行归纳分析。结果与结论:(1)外泌体通过胞吞胞吐膜融合方式输送物质至受体细胞或膜表面携带信号因子,释放在细胞间隙等方式在细胞间通讯进行物质交换。(2)外泌体可通过介导细胞间通讯和信息交流以及物质运输参与帕金森病的病理进展,因此其可作为帕金森病的早期生物标志物,协助提供新的诊疗靶点,并可作为将来帕金森病的诊疗载体。(3)外泌体通过细胞间传递和物质运输扩散介导参与帕金森病病理发展机制,其主要生物标志物与病理作用途径的关系为:外泌体α突触核蛋白介导神经毒性、神经炎症、氧化应激及细胞自噬等过程,外泌体微小RNA可以靶向帕金森病相关基因并参与氧化应激,外泌体DJ-1蛋白调控细胞的氧化应激水平并影响细胞毒性,外泌体富含亮氨酸的重复激酶2介导路易体形成和堆积造成神经毒性,以上外泌体及其携带物质均可作为帕金森病的早期生物标志物。(4)目前外泌体的帕金森病生物标志物相关研究较少,但其中最具代表性和应用最多的是外泌体α突触核蛋白,由于外泌体微小RNA种类多数量大,在帕金森病中表达和作用不尽相同,近两年成为了研究热点。(5)目前相关展开更多
Surgery is the comm on treatme nt for early lung cancer with multiple pulm onary no dules,but it is often accompanied by the problem of significant malignancy of other nodules in non-therapeutic areas.In this study,we...Surgery is the comm on treatme nt for early lung cancer with multiple pulm onary no dules,but it is often accompanied by the problem of significant malignancy of other nodules in non-therapeutic areas.In this study,we found that a combined treatment of local rad iofreq ue ncy ablati on(RFA)and melatonin(MLT)greatly improved clinical outcomes for early lung cancer patie nts with multiple pulmonary nodules by minimizing lung function injury and reducing the probability of malignant transformation or enlargement of nodules in non-ablated areas.Mechanically,as demonstrated in an associated mouse lung tumor model,RFA not only effectively remove treated tumors but also stimulate antitumor immunity,which could inhibit tumor growth in non-ablated areas.MLT enhanced RFA-stimulated NK activity and exerted synergistic antitumor effects with RFA.Transcriptomics and proteomics analyses of residual tumor tissues revealed enhanced oxidative phosphorylation and reduced acidification as well as hypoxia in the tumor microenvironment,which suggests reprogrammed tumor metabolism after combined treatment with RFA and MLT.Analysis of residual tumor further revealed the depressed activity of MAPK,NF-kappa B,Wnt,and Hedgehog pathways and upregulated P53 pathway in tumors,which was in line with the inhibited tumor growth.Combined RFA and MLT treatment also reversed the Warburg effect and decreased tumor malignancy.These findings thus demonstrated that combined treatment of RFA and MLT effectively inhibited the malignancy of non-ablated nodules and provided an innovative non-invasive strategy for treating early lung tumors with multiple pulmonary nodules.Trial registration:www.chictr.org.cn,identifier ChiCTR2100042695,http://www.chictr.org.cn/showproj.aspx?proj=120931.展开更多
基金funded by Beijing Municipal Natural Science Foundation(Grant No.6172017),ChinaOpening Foundation of Key Laboratory of Biomass Energy and Materials of Jiangsu Province(JSBEM2016013),ChinaNational Key Research and Development Program of China(2017YFD0500500),China.
文摘This study was conducted to evaluate the effects of sporoderm-broken spores of Ganoderma lucidum(SSGL),a traditional Chinese medicinal herb,on growth performance,antioxidant ability,and immunity of broilers.Three hundred male broilers with similar body weights(40.0±1.0 g)at 1 d of age were assigned randomly to 4 treatments.Each treatment contained 5 replicates of 15 birds per replicate.The dietary treatments were corn-soybean meal basal diet supple mented with SSGL at the concentrations of0(control),100,200 and 500 mg/kg diet.The results showed that diets supplemented with SSGL significantly increased(P<0.05)the average daily gain and decreased(P<0.05)the feed:gain(F:G)ratio of birds during the finisher period(22 to 44 d of age).Moreover,the total antioxidant capability,glutathione reductase and catalase activities in the liver and spleen were significantly higher(P<0.05)in broilers fed diets with SSGL than in broilers fed the control diet.Additionally,dietary SSGL also increased(P<0.05)the serum interleukin(IL)-2,immunoglobulin(Ig)A and IgG levels of broilers compared with the control diet.These results suggest that SSGL have ameliorative effects on growth performance,free radical-scavenging activity,antioxidant capability,and immune function of broilers.
基金supported by National Natural Science Foundation of China(Grant No.31772637,31301981)a Special Fund for Agro-scientific Research in the Public Interest(201403047)+1 种基金Science and Technology Project of Fuzhou City(2017N0033)China Postdoctoral Science Foundation Grant(Grant No.2016 M600871)
文摘Background: The current study was carried out to evaluate the effects of mycotoxin biodegradation agent(MBA, composed of Bacillus subtilis ANSB01 G and Devosia sp. ANSB714) on relieving zearalenone(ZEA) and deoxynivalenol(DON) toxicosis in immature gilts.Methods: A total of forty pre-pubertal female gilts(61.42 ± 1.18 kg) were randomly allocated to four diet treatments: CO(positive control); MO(negative control, ZEA 596.86 μg/kg feed and DON 796 μg/kg feed);COA(CO + 2 g MBA/kg feed); MOA(MO + 2 g MBA/kg feed). Each treatment contained 10 replicates with 1 gilt per replicate. Gilts were housed in an environmentally controlled room with the partially slatted floor.Results: During the entire experimental period of 28 d, average daily gain(ADG) and average daily feed intake(ADFI)of gilts in MO group was significantly reduced compared with those in CO group. The vulva size of gilts was significantly higher in MO group than CO group. In addition, significant increases in the plasma levels of Ig A,Ig G, IL-8, IL-10 and PRL were determined in MO group compared with that in CO group. ZEA and DON in the diet upregulated apoptotic caspase-3 in ovaries and uteri, along with down-regulated the anti-apoptotic protein Bcl-2 in ovaries. The supplementation of MBA into diets co-contaminated with ZEA and DON significantly increased ADG, decreased the vulva sizes, reduced the levels of Ig G, IL-8 and PRL in plasma, and regulated apoptosis in ovaries and uteri of gilts.Conclusions: The present results indicated that feeding diet contaminated with ZEA and DON simultaneously(596.86 μg/kg + 796 μg/kg) had detrimental effects on growth performance, plasma immune function and reproductive status of gilts. And MBA could reduce the negative impacts of these two toxins, believed as a promising feed additive for mitigating toxicosis of ZEA and DON at low levels in gilts.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 49999560)the National Key Basic Research Special Foundation Project (Grant No. 2000078500)the Knowledge Innovation Project of the Chinese Academy of Sciences (Gran
文摘This note studies the Radiolarian fossil groups since 1.2 MaBP in ODP leg 184 site 1143, the southern South China Sea (SCS). The result shows that radiolarian abundance experienced a significant variation: before 0.9 MaBP it remained at the extremely low level, but increased with low extent between 0.9-0.65 Ma, which corresponded to the Mid-Pleistocene Transition stage, and it increased rapidly after 0.65 MaBP. During the whole process, the average abundance became higher and higher in each stage, and showed regularly periodic fluctuations. The obvious increase after 0.65 MaBP is inferred to result from the enhanced up-welling in this region, which was induced by the intensified monsoon circulation after the 'Mid-Pleistocene Transition'. An outstanding result of the spectral analyses is that a long oscillation of - 0.2 Ma cycle was found in the records of radiolarian abundance and complex diversity, which corresponds well to the result of other paleoceanographic indexes. This probably indicated a
文摘According to multi- disciplinary research advances in recent years, three blocks which named Jiaoliao - Bohai block, central North China block and Ordos block are divided. The differences of the tectonic association, types, styles and features in the space, and evolution from Archaean to Cenozoic in the time, between the former two blocks are discussed. Tectonic features and evolution in the four tectonic stages of Precambrian, Indo- China, Yanshanian and Cenozoic are emphasized especially here. All of them described above have inherit and neogenic property. The temporal-spatial change and interaction among basin assemblages in the above four stages are analyzed. Escape tectonic model is employed to interpret coeval compressive and extensional basins in the study area.
文摘背景:帕金森病是全球第二大神经退行性疾病,其运动症状和非运动症状都严重影响患者日常生活,最近研究发现外泌体及其携带的物质参与帕金森病病理发生发展,且还介导细胞通讯可作为帕金森病的潜在生物标志物。目的:探讨外泌体介导细胞通讯作为帕金森病的潜在生物标志物的类型及其发生作用的机制,用以寻找新的早期诊疗靶点,以期减缓帕金森病的发病进程并为其诊疗提供新思路。方法:应用计算机检索1987年1月至2022年8月PubMed、中国知网、万方数据、维普、Web of Science、EMBASE、Cochrane、EBSCO和SinoMed数据库收录的相关文献,最终纳入76篇英文文献和2篇中文文献进行归纳分析。结果与结论:(1)外泌体通过胞吞胞吐膜融合方式输送物质至受体细胞或膜表面携带信号因子,释放在细胞间隙等方式在细胞间通讯进行物质交换。(2)外泌体可通过介导细胞间通讯和信息交流以及物质运输参与帕金森病的病理进展,因此其可作为帕金森病的早期生物标志物,协助提供新的诊疗靶点,并可作为将来帕金森病的诊疗载体。(3)外泌体通过细胞间传递和物质运输扩散介导参与帕金森病病理发展机制,其主要生物标志物与病理作用途径的关系为:外泌体α突触核蛋白介导神经毒性、神经炎症、氧化应激及细胞自噬等过程,外泌体微小RNA可以靶向帕金森病相关基因并参与氧化应激,外泌体DJ-1蛋白调控细胞的氧化应激水平并影响细胞毒性,外泌体富含亮氨酸的重复激酶2介导路易体形成和堆积造成神经毒性,以上外泌体及其携带物质均可作为帕金森病的早期生物标志物。(4)目前外泌体的帕金森病生物标志物相关研究较少,但其中最具代表性和应用最多的是外泌体α突触核蛋白,由于外泌体微小RNA种类多数量大,在帕金森病中表达和作用不尽相同,近两年成为了研究热点。(5)目前相关
基金supported by the National Natural Science Foundation of China(Nos.31770131,81473469)International Cooperation Project of the Belt and Road(No.20400750600)+1 种基金Shanghai Shen Kang Hospital Development Center Plan(SHDC12018119)Shanghai Municipal Commission of Health and Family Plan(201840056).
文摘Surgery is the comm on treatme nt for early lung cancer with multiple pulm onary no dules,but it is often accompanied by the problem of significant malignancy of other nodules in non-therapeutic areas.In this study,we found that a combined treatment of local rad iofreq ue ncy ablati on(RFA)and melatonin(MLT)greatly improved clinical outcomes for early lung cancer patie nts with multiple pulmonary nodules by minimizing lung function injury and reducing the probability of malignant transformation or enlargement of nodules in non-ablated areas.Mechanically,as demonstrated in an associated mouse lung tumor model,RFA not only effectively remove treated tumors but also stimulate antitumor immunity,which could inhibit tumor growth in non-ablated areas.MLT enhanced RFA-stimulated NK activity and exerted synergistic antitumor effects with RFA.Transcriptomics and proteomics analyses of residual tumor tissues revealed enhanced oxidative phosphorylation and reduced acidification as well as hypoxia in the tumor microenvironment,which suggests reprogrammed tumor metabolism after combined treatment with RFA and MLT.Analysis of residual tumor further revealed the depressed activity of MAPK,NF-kappa B,Wnt,and Hedgehog pathways and upregulated P53 pathway in tumors,which was in line with the inhibited tumor growth.Combined RFA and MLT treatment also reversed the Warburg effect and decreased tumor malignancy.These findings thus demonstrated that combined treatment of RFA and MLT effectively inhibited the malignancy of non-ablated nodules and provided an innovative non-invasive strategy for treating early lung tumors with multiple pulmonary nodules.Trial registration:www.chictr.org.cn,identifier ChiCTR2100042695,http://www.chictr.org.cn/showproj.aspx?proj=120931.
