Objective: There are no comprehensive studies on survival outcomes and optimal treatment protocols for cervical esophageal cancer(CEC), due to its rare clinical prevalence. Our objective was to determine the relations...Objective: There are no comprehensive studies on survival outcomes and optimal treatment protocols for cervical esophageal cancer(CEC), due to its rare clinical prevalence. Our objective was to determine the relationship between pathological characteristics, treatment protocols, and survival outcomes in Chinese CEC patients.Methods: A total of 500 Chinese CEC patients were selected from our 500,000 esophageal and gastric cardia carcinoma database(1973–2018). There were two main groups: patients treated with surgery, and patients receiving non-surgical treatments(radiotherapy, radiochemotherapy, and chemotherapy). The Chi-square test and Kaplan–Meier method were used to compare the continuous variables and survival.Results: Among the 500 CEC patients, 278(55.6%) were male, and the median age was 60.9 ± 9.4 years. A total of 496 patients(99.2%) were diagnosed with squamous cell carcinoma. In 171(34.2%) patients who received surgery, 22(12.9%) had undergone laryngectomy. In 322(64.4%) patients who received non-surgical treatments, 245(76.1%) received radiotherapy. Stratified survival analysis showed that only T stage was related with survival outcomes for CEC patients in the surgical group, and the outcomes between laryngectomy and non-laryngectomy patients were similar. It was noteworthy that the 5-year survival rate was similar in CEC patients among the different groups treated with surgery, radiotherapy, chemotherapy, or radiochemotherapy(P = 0.244). Conclusions: The CEC patients had similar survival outcomes after curative esophagectomy and radiotherapy, including those with or without total laryngectomy. These findings suggest that radiotherapy could be the initial choice for treatment of Chinese CEC patients.展开更多
Traditional antifouling agents usually have a certain toxic effect on marine environments and non-target organisms.In this study,Dictyophora indusiata polysaccharide(DIP)was applied as a natural antifouling surface mo...Traditional antifouling agents usually have a certain toxic effect on marine environments and non-target organisms.In this study,Dictyophora indusiata polysaccharide(DIP)was applied as a natural antifouling surface modifier to prepare the surface coating for marine antifouling.Three DIP coatings were prepared:D.indusiata spore polysaccharide(DISP),D.indusiata volva polysaccharide(DIVP),and D.indusiata embryonic body polysaccharide(DIEP).The antifouling,tribological,and anticorrosion behavior of the coatings were examined.Results revealed that the three kinds of DIP coatings had excellent antifouling properties,which could effectively prevent the adhesion of Chlorella and the attachment of water-based and oily stains on the surface.Additionally,the coatings showed great mechanical stability and could maintain an extremely low coefficient of friction(COF<0.05)after continuous wear.The drag reduction rate of the coated surfaces reached 5%,showing a powerful lubrication performance.Furthermore,the DIP coatings presented an outstanding corrosion resistance,where the equivalent circuit impedances were 4-9 orders of magnitude higher than the control groups.This research showed a promising prospect of surface coating fabrication with DIP for marine devices to achieve the purpose of antifouling and drag reduction.展开更多
目的:基于网络药理学及分子对接探讨丹红通精方治疗男性不育症的作用机制。方法:基于中国知网、万方数据、中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP,h...目的:基于网络药理学及分子对接探讨丹红通精方治疗男性不育症的作用机制。方法:基于中国知网、万方数据、中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP,http://tcmspw.com/tcmsp.php)等数据库检索丹红通精方组方药物的主要有效成分,通过TCMSP检索有效成分相关靶点。运用Genecards、DisGeNET和在线人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)检索不育相关靶点。通过Venny 2.1软件对丹红通精方的有效活性成分靶点和丹红通精方靶点进行映射,筛选出共同靶点,即为丹红通精方治疗不育的潜在靶点,绘制韦恩图。将潜在靶点导入String数据库,得到潜在靶点的蛋白相互关系(protein-protein interaction,PPI),并构建PPI网络。将有效成分、潜在靶点导入Cytoscape 3.8.2软件构建“药物-有效成分-潜在靶点-疾病”网络,并采用Network Analyzer进行拓扑分析筛选核心有效成分。运用R软件进行潜在靶点的基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)信号通路富集分析。采用AutoDock Vina对关键成分和靶点进行分子对接。结果:通过检索各数据库获得丹红通精方有效成分264个,相关靶点313个。运用Genecards、DisGeNET和OMIM数据库获得不育相关靶点3765个。通过Venny 2.1软件筛选出203个潜在靶点。“药物-有效成分-潜在靶点-疾病”网络拓扑分析筛选得到槲皮素、山柰酚、β-谷甾醇等核心成分。PPI网络分析得到JUN、过氧化物酶体增殖物激活受体(peroxisome proliferators-activated receptors,PPARs)、白细胞介素-6(interleukin-6,IL-6)、核转录因子-κB抑制剂α(nuclear transcription factor-κB inhibitor alpha,NF-κBIα)等核心靶点。KEGG信号通路富集分析得到157条信号通路。分子对接显示核心成分与核心蛋白结合良好�展开更多
基金supported by the National Key R&D Program “Precision Medicine” of China (Grant No. 2016YFC0901403)the Major Science and Technology Projects of Henan Province (Grant No. 16110031 1300)+2 种基金the Doctoral Team Foundation of the First Affiliated Hospital of Zhengzhou University (Grant No. 2016-BSTDJJ-03)the National Natural Science Foundation of China (Grant No. 81872032, U1804262)the State Key Laboratory of Esophageal Cancer Prevention and Treatment (Grant No. Z2020-0010)。
文摘Objective: There are no comprehensive studies on survival outcomes and optimal treatment protocols for cervical esophageal cancer(CEC), due to its rare clinical prevalence. Our objective was to determine the relationship between pathological characteristics, treatment protocols, and survival outcomes in Chinese CEC patients.Methods: A total of 500 Chinese CEC patients were selected from our 500,000 esophageal and gastric cardia carcinoma database(1973–2018). There were two main groups: patients treated with surgery, and patients receiving non-surgical treatments(radiotherapy, radiochemotherapy, and chemotherapy). The Chi-square test and Kaplan–Meier method were used to compare the continuous variables and survival.Results: Among the 500 CEC patients, 278(55.6%) were male, and the median age was 60.9 ± 9.4 years. A total of 496 patients(99.2%) were diagnosed with squamous cell carcinoma. In 171(34.2%) patients who received surgery, 22(12.9%) had undergone laryngectomy. In 322(64.4%) patients who received non-surgical treatments, 245(76.1%) received radiotherapy. Stratified survival analysis showed that only T stage was related with survival outcomes for CEC patients in the surgical group, and the outcomes between laryngectomy and non-laryngectomy patients were similar. It was noteworthy that the 5-year survival rate was similar in CEC patients among the different groups treated with surgery, radiotherapy, chemotherapy, or radiochemotherapy(P = 0.244). Conclusions: The CEC patients had similar survival outcomes after curative esophagectomy and radiotherapy, including those with or without total laryngectomy. These findings suggest that radiotherapy could be the initial choice for treatment of Chinese CEC patients.
基金National Natural Science Foundation of China(52375298,51975458,and 51905370)support from the China Scholarship Council,China Postdoctoral Science Foundation funded project(2020M673377 and 2020T130510)+1 种基金Key R&D program of Shaanxi Province(2022SF-069)the Natural Science Fund of Shaanxi Province(2020JM-010).
文摘Traditional antifouling agents usually have a certain toxic effect on marine environments and non-target organisms.In this study,Dictyophora indusiata polysaccharide(DIP)was applied as a natural antifouling surface modifier to prepare the surface coating for marine antifouling.Three DIP coatings were prepared:D.indusiata spore polysaccharide(DISP),D.indusiata volva polysaccharide(DIVP),and D.indusiata embryonic body polysaccharide(DIEP).The antifouling,tribological,and anticorrosion behavior of the coatings were examined.Results revealed that the three kinds of DIP coatings had excellent antifouling properties,which could effectively prevent the adhesion of Chlorella and the attachment of water-based and oily stains on the surface.Additionally,the coatings showed great mechanical stability and could maintain an extremely low coefficient of friction(COF<0.05)after continuous wear.The drag reduction rate of the coated surfaces reached 5%,showing a powerful lubrication performance.Furthermore,the DIP coatings presented an outstanding corrosion resistance,where the equivalent circuit impedances were 4-9 orders of magnitude higher than the control groups.This research showed a promising prospect of surface coating fabrication with DIP for marine devices to achieve the purpose of antifouling and drag reduction.
文摘目的:基于网络药理学及分子对接探讨丹红通精方治疗男性不育症的作用机制。方法:基于中国知网、万方数据、中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP,http://tcmspw.com/tcmsp.php)等数据库检索丹红通精方组方药物的主要有效成分,通过TCMSP检索有效成分相关靶点。运用Genecards、DisGeNET和在线人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)检索不育相关靶点。通过Venny 2.1软件对丹红通精方的有效活性成分靶点和丹红通精方靶点进行映射,筛选出共同靶点,即为丹红通精方治疗不育的潜在靶点,绘制韦恩图。将潜在靶点导入String数据库,得到潜在靶点的蛋白相互关系(protein-protein interaction,PPI),并构建PPI网络。将有效成分、潜在靶点导入Cytoscape 3.8.2软件构建“药物-有效成分-潜在靶点-疾病”网络,并采用Network Analyzer进行拓扑分析筛选核心有效成分。运用R软件进行潜在靶点的基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)信号通路富集分析。采用AutoDock Vina对关键成分和靶点进行分子对接。结果:通过检索各数据库获得丹红通精方有效成分264个,相关靶点313个。运用Genecards、DisGeNET和OMIM数据库获得不育相关靶点3765个。通过Venny 2.1软件筛选出203个潜在靶点。“药物-有效成分-潜在靶点-疾病”网络拓扑分析筛选得到槲皮素、山柰酚、β-谷甾醇等核心成分。PPI网络分析得到JUN、过氧化物酶体增殖物激活受体(peroxisome proliferators-activated receptors,PPARs)、白细胞介素-6(interleukin-6,IL-6)、核转录因子-κB抑制剂α(nuclear transcription factor-κB inhibitor alpha,NF-κBIα)等核心靶点。KEGG信号通路富集分析得到157条信号通路。分子对接显示核心成分与核心蛋白结合良好�
