Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CF...Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.展开更多
<b>Objectives:</b> To understand sperm motility in adults and its association with lifestyle in an ethnic minority area in Southwest China. </span></span><span lang="EN-US" style=&...<b>Objectives:</b> To understand sperm motility in adults and its association with lifestyle in an ethnic minority area in Southwest China. </span></span><span lang="EN-US" style="white-space:normal;" "=""><b><span>Methods:</span></b><span> A hospital-based cross-sectional study to assess sperm motility in male adults was conducted at the Reproductive Health Center from January 2018 to May 2019. </span></span><span lang="EN-US" style="white-space:normal;" "="">The </span><span lang="EN-US" style="white-space:normal;" "="">dat</span><span lang="EN-US" style="white-space:normal;" "="">a</span><span lang="EN-US" style="white-space:normal;" "=""><span> was collected with a questionnaire and semen quality was analyzed with Computer-Aided Sperm Analysis system (CASA). Analysis of covariance (ANCOVA) was used to measure the relationship between lifestyle factors and sperm motility. </span></span><span lang="EN-US" style="white-space:normal;" "=""><span></span><b><span>Results:</span></b></span><span lang="EN-US" style="white-space:normal;" "=""><span> A total of 349 people were recruited. Dietary celery intake was significantly related to the increase of sperm progressive motility and total motility (</span><i><span>β</span></i><span> = 7.00, 95% CI: 1.59, 12.42 and </span><i><span>β</span></i><span> = 7.26, 95% CI: 1.45, 13.07, respectively). Cola consumption was associated with increased sperm progressive motility (</span><i><span>β</span></i><span> = 9.71, 95% CI: 1.46, 17.96). Frequent use of plastic bags for meat food storage (</span><i><span>β</span></i><span> = -5.56, 95% CI: -10.61, -0.51), industry work (</span><i><span>β</span></i><span> = -</span></span><span lang="EN-US" style="white-space:normal;" "="">5.64, 95% CI: -</span><span lang="EN-US" style="white-space:normal;" "="">11.21, -</span><span lang="EN-US" style="white-space:normal;" "=""><span>0.07), organic disease (</span><i><span>β</span></i><span> = -</span></span><span lang="EN-US" style="white-space:normal;" "="">6.14, 95% CI: -</span><span l展开更多
文摘Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.
文摘<b>Objectives:</b> To understand sperm motility in adults and its association with lifestyle in an ethnic minority area in Southwest China. </span></span><span lang="EN-US" style="white-space:normal;" "=""><b><span>Methods:</span></b><span> A hospital-based cross-sectional study to assess sperm motility in male adults was conducted at the Reproductive Health Center from January 2018 to May 2019. </span></span><span lang="EN-US" style="white-space:normal;" "="">The </span><span lang="EN-US" style="white-space:normal;" "="">dat</span><span lang="EN-US" style="white-space:normal;" "="">a</span><span lang="EN-US" style="white-space:normal;" "=""><span> was collected with a questionnaire and semen quality was analyzed with Computer-Aided Sperm Analysis system (CASA). Analysis of covariance (ANCOVA) was used to measure the relationship between lifestyle factors and sperm motility. </span></span><span lang="EN-US" style="white-space:normal;" "=""><span></span><b><span>Results:</span></b></span><span lang="EN-US" style="white-space:normal;" "=""><span> A total of 349 people were recruited. Dietary celery intake was significantly related to the increase of sperm progressive motility and total motility (</span><i><span>β</span></i><span> = 7.00, 95% CI: 1.59, 12.42 and </span><i><span>β</span></i><span> = 7.26, 95% CI: 1.45, 13.07, respectively). Cola consumption was associated with increased sperm progressive motility (</span><i><span>β</span></i><span> = 9.71, 95% CI: 1.46, 17.96). Frequent use of plastic bags for meat food storage (</span><i><span>β</span></i><span> = -5.56, 95% CI: -10.61, -0.51), industry work (</span><i><span>β</span></i><span> = -</span></span><span lang="EN-US" style="white-space:normal;" "="">5.64, 95% CI: -</span><span lang="EN-US" style="white-space:normal;" "="">11.21, -</span><span lang="EN-US" style="white-space:normal;" "=""><span>0.07), organic disease (</span><i><span>β</span></i><span> = -</span></span><span lang="EN-US" style="white-space:normal;" "="">6.14, 95% CI: -</span><span l
