Danshen-Chuanxiongqin Injection(DCI)is a commonly used traditional Chinese medicine for the treatment of cerebral ischemic stroke in China.However,its underlying mechanisms remain completely understood.The current stu...Danshen-Chuanxiongqin Injection(DCI)is a commonly used traditional Chinese medicine for the treatment of cerebral ischemic stroke in China.However,its underlying mechanisms remain completely understood.The current study was designed to explore the protective mechanisms of DCI against cerebral ischemic stroke through integrating whole-transcriptome sequencing coupled with network pharmacology analysis.First,using a mouse model of cerebral ischemic stroke by transient middle cerebral artery occlusion(tMCAO),we found that DCI(4.10 mL·kg−1)significantly alleviated cerebral ischemic infarction,neurological deficits,and the pathological injury of hippocampal and cortical neurons in mice.Next,the whole-transcriptome sequencing was performed on brain tissues.The cerebral ischemia disease(CID)network was constructed by integrating transcriptome sequencing data and cerebrovascular disease-related genes.The results showed CID network was imbalanced due to tMCAO,but a recovery regulation was observed after DCI treatment.Pathway analysis of the key genes with recovery efficiency showed that the neuroinflammation signaling pathway was highly enriched,while the TLR2/TLR4-MyD88-NF-κB pathway was predicted to be affected.Consistently,the in vivo validation experiments confirmed that DCI exhibited protective effects against cerebral ischemic stroke by inhibiting neuroinflammation via the TLR2/TLR4-MyD88-NF-κB pathway.More interestingly,DCI markedly suppressed the neutrophils infiltrated into the brain parenchyma via the choroid plexus route and showed anti-neuroinflammation effects.In conclusion,our results provide dependable evidence that inhibiting neuroinflammation via the TLR2/TLR4-MyD88-NF-κB pathway is the main mechanism of DCI against cerebral ischemic stroke in mice.展开更多
Sym-bis（benzimidazole）-2,2＇-ethylene cations act as a new axle template for threading cucurbit[6]uril derivatives on,forming[2]pseudorotaxane and[3]pseudorotaxane.These new complexes have been studied using 1 H NMR...Sym-bis（benzimidazole）-2,2＇-ethylene cations act as a new axle template for threading cucurbit[6]uril derivatives on,forming[2]pseudorotaxane and[3]pseudorotaxane.These new complexes have been studied using 1 H NMR,UV-vis absorption spectroscopy and X-ray analysis.Changes in the 1 H NMR spectra indicate that the two types of pseudorotaxane can be formed by varying the host concentration. UV-vis absorption titration experiments at different pH values demonstrate that interesting pK_a shifts of the bis-benzimidazole derivatives can be induced by the host-guest complexation.The associated constants were calculated to be 2.81×10^4 L/mol and 9.06×10^6 L/mol for the[2]pseudorotaxanes and ｜3]pseudorotaxanes,respectively.Furthermore,X-ray diffraction studies of the solid state structures provide unequivocal proof of the host concentration dependent pseudorotaxane,which is strongly in line with the evidences in solution.展开更多
采用900 e V能量的电子对直玻璃管进行了穿透实验,测量了玻璃管在倾角为–0.15°,–0.4°和–1.15°时充电过程角分布的时间演化,以及平衡态下出射电子能谱.发现穿透率随时间先下降后上升最后趋于平稳,下降的时间随倾角的...采用900 e V能量的电子对直玻璃管进行了穿透实验,测量了玻璃管在倾角为–0.15°,–0.4°和–1.15°时充电过程角分布的时间演化,以及平衡态下出射电子能谱.发现穿透率随时间先下降后上升最后趋于平稳,下降的时间随倾角的增大而减小.当倾角为–0.4°和–1.15°时,电子穿透率下降到最低点时几乎看不到穿透电子(穿透率小于3‰),这种穿透率最低点状态保持时间随倾角增大而增大.穿透电子的角分布中心随着时间变化.在平稳状态时,发现穿透电子的能量损失随倾角增大而增大.采用蒙特卡罗方法模拟了电子经过管壁不同次数反射后的能谱,与测量能谱进行对比,发现–0.15°,–0.4°和–1.15°倾角下,穿透电子分别经历了管壁的一次、两次和三次与表面的反射过程.基于此,本文对电子穿越玻璃管的充电过程动力学给出了物理解释.实验结果和理论分析表明,在小倾角下玻璃管内能形成宏观负电荷累积,排斥后续电子形成反射,增加电子出射概率,这对应用绝缘体微结构,例如玻璃锥管产生稳定的电子微束具有重要的参考意义.展开更多
Objective： To explore the relationships of the polymorphisms of xeroderma pigmentosum C （XPC） and the susceptibility of esophageal cancer （EC） in Changzhi area, China. Methods： The study was conducted by a case...Objective： To explore the relationships of the polymorphisms of xeroderma pigmentosum C （XPC） and the susceptibility of esophageal cancer （EC） in Changzhi area, China. Methods： The study was conducted by a case-control study which included 196 cases of EC and 201 cases of controls. XPC PAT polymorphisms were determined with polymerase chain-restriction on fragment length polymorphism （PCR-RFLP）. Results： The frequencies of wild homozygote （PAT-/-）, mutation heterozygote （PAT-/＋） and mutation homozygote （PAT＋/＋） of XPC were 36.73%, 51.53% and 11.74% in case group, 37.81%, 52.24% and 9.95% in control group, respectively, and there was no significant difference between the two groups （X^2 =0.332, P=0.847）. There was no interaction between XPC PAT mutation genotype and xeroderma pigmentosum A （XPA） （S=0.85） and pickled food （S=0.81）. Conclusion： A genetic polymorphism in XPC may be not associated with esophageal cancer in Changzhi population.展开更多
In this paper, the turbulence characteristics were numerically investigatedin an asymmetric turbulent channel flow and the computational results were compared with therelevant experimental data. It shows that the resu...In this paper, the turbulence characteristics were numerically investigatedin an asymmetric turbulent channel flow and the computational results were compared with therelevant experimental data. It shows that the results are consistent with the experiments and thereexist Counter-Gradient Momentum Transport (CGMT) phenomena in the central region near the smoothwall, and this region is as large as 6 percent of the channel width. In addition, a region, in whichCounter-Gradient-Transport (CGT) phenomena occur more evidently, is found close to the rough wall.These results can help to gain a deeper insight into the mechanism of CGT phenomena.展开更多
基金supported by the National S&T Major Project(No.2018ZX09201011)the National Youth Topnotch Talent Support Program(No.W02070098).
文摘Danshen-Chuanxiongqin Injection(DCI)is a commonly used traditional Chinese medicine for the treatment of cerebral ischemic stroke in China.However,its underlying mechanisms remain completely understood.The current study was designed to explore the protective mechanisms of DCI against cerebral ischemic stroke through integrating whole-transcriptome sequencing coupled with network pharmacology analysis.First,using a mouse model of cerebral ischemic stroke by transient middle cerebral artery occlusion(tMCAO),we found that DCI(4.10 mL·kg−1)significantly alleviated cerebral ischemic infarction,neurological deficits,and the pathological injury of hippocampal and cortical neurons in mice.Next,the whole-transcriptome sequencing was performed on brain tissues.The cerebral ischemia disease(CID)network was constructed by integrating transcriptome sequencing data and cerebrovascular disease-related genes.The results showed CID network was imbalanced due to tMCAO,but a recovery regulation was observed after DCI treatment.Pathway analysis of the key genes with recovery efficiency showed that the neuroinflammation signaling pathway was highly enriched,while the TLR2/TLR4-MyD88-NF-κB pathway was predicted to be affected.Consistently,the in vivo validation experiments confirmed that DCI exhibited protective effects against cerebral ischemic stroke by inhibiting neuroinflammation via the TLR2/TLR4-MyD88-NF-κB pathway.More interestingly,DCI markedly suppressed the neutrophils infiltrated into the brain parenchyma via the choroid plexus route and showed anti-neuroinflammation effects.In conclusion,our results provide dependable evidence that inhibiting neuroinflammation via the TLR2/TLR4-MyD88-NF-κB pathway is the main mechanism of DCI against cerebral ischemic stroke in mice.
基金supported by the National Natural Science Foundation of China(No,21272045)the Natural Science Foundation of Guizhou Province and Guizhou Universitythe "Chun-Hui" Funds of the Chinese Ministry of Education are gratefully acknowledged
文摘Sym-bis（benzimidazole）-2,2＇-ethylene cations act as a new axle template for threading cucurbit[6]uril derivatives on,forming[2]pseudorotaxane and[3]pseudorotaxane.These new complexes have been studied using 1 H NMR,UV-vis absorption spectroscopy and X-ray analysis.Changes in the 1 H NMR spectra indicate that the two types of pseudorotaxane can be formed by varying the host concentration. UV-vis absorption titration experiments at different pH values demonstrate that interesting pK_a shifts of the bis-benzimidazole derivatives can be induced by the host-guest complexation.The associated constants were calculated to be 2.81×10^4 L/mol and 9.06×10^6 L/mol for the[2]pseudorotaxanes and ｜3]pseudorotaxanes,respectively.Furthermore,X-ray diffraction studies of the solid state structures provide unequivocal proof of the host concentration dependent pseudorotaxane,which is strongly in line with the evidences in solution.
文摘采用900 e V能量的电子对直玻璃管进行了穿透实验,测量了玻璃管在倾角为–0.15°,–0.4°和–1.15°时充电过程角分布的时间演化,以及平衡态下出射电子能谱.发现穿透率随时间先下降后上升最后趋于平稳,下降的时间随倾角的增大而减小.当倾角为–0.4°和–1.15°时,电子穿透率下降到最低点时几乎看不到穿透电子(穿透率小于3‰),这种穿透率最低点状态保持时间随倾角增大而增大.穿透电子的角分布中心随着时间变化.在平稳状态时,发现穿透电子的能量损失随倾角增大而增大.采用蒙特卡罗方法模拟了电子经过管壁不同次数反射后的能谱,与测量能谱进行对比,发现–0.15°,–0.4°和–1.15°倾角下,穿透电子分别经历了管壁的一次、两次和三次与表面的反射过程.基于此,本文对电子穿越玻璃管的充电过程动力学给出了物理解释.实验结果和理论分析表明,在小倾角下玻璃管内能形成宏观负电荷累积,排斥后续电子形成反射,增加电子出射概率,这对应用绝缘体微结构,例如玻璃锥管产生稳定的电子微束具有重要的参考意义.
基金supported by a grant from Scholarship Council Fund of Shanxi Province (No.200565)
文摘Objective： To explore the relationships of the polymorphisms of xeroderma pigmentosum C （XPC） and the susceptibility of esophageal cancer （EC） in Changzhi area, China. Methods： The study was conducted by a case-control study which included 196 cases of EC and 201 cases of controls. XPC PAT polymorphisms were determined with polymerase chain-restriction on fragment length polymorphism （PCR-RFLP）. Results： The frequencies of wild homozygote （PAT-/-）, mutation heterozygote （PAT-/＋） and mutation homozygote （PAT＋/＋） of XPC were 36.73%, 51.53% and 11.74% in case group, 37.81%, 52.24% and 9.95% in control group, respectively, and there was no significant difference between the two groups （X^2 =0.332, P=0.847）. There was no interaction between XPC PAT mutation genotype and xeroderma pigmentosum A （XPA） （S=0.85） and pickled food （S=0.81）. Conclusion： A genetic polymorphism in XPC may be not associated with esophageal cancer in Changzhi population.
文摘In this paper, the turbulence characteristics were numerically investigatedin an asymmetric turbulent channel flow and the computational results were compared with therelevant experimental data. It shows that the results are consistent with the experiments and thereexist Counter-Gradient Momentum Transport (CGMT) phenomena in the central region near the smoothwall, and this region is as large as 6 percent of the channel width. In addition, a region, in whichCounter-Gradient-Transport (CGT) phenomena occur more evidently, is found close to the rough wall.These results can help to gain a deeper insight into the mechanism of CGT phenomena.
