目的探讨经阴道子宫输卵管实时三维超声造影(RT-3D-Hy Co Sy)和X线碘水造影(HSG)在不孕症临床诊治中的应用价值。方法回顾性分析2017年5~11月在云浮市人民医院就诊的不孕症患者的临床资料,以腹腔镜输卵管通液术(LTF)为金标准,比较RT-3D-...目的探讨经阴道子宫输卵管实时三维超声造影(RT-3D-Hy Co Sy)和X线碘水造影(HSG)在不孕症临床诊治中的应用价值。方法回顾性分析2017年5~11月在云浮市人民医院就诊的不孕症患者的临床资料,以腹腔镜输卵管通液术(LTF)为金标准,比较RT-3D-Hy CoSy和HSG评估输卵管通畅程度和输卵管有无积水的准确率,分析两种技术的临床应用价值。结果 121例患者进行3D-Hy Co Sy检查,170例患者进行HSG检查,38例患者进行腹腔镜输卵管通液术治疗。对输卵管通畅程度的评估,3D-Hy CoSy、HSG与LTF的总符合率分别为85.5%(κ=0.735)和78.9%(κ=0.652);对输卵管有无积水的评估,3D-Hy CoSy、HSG与LTF的总符合率分别为81.6%(κ=0.685)和80.3%(κ=0.643)。两种技术诊断输卵管通畅程度和有无积水的准确率差异均无统计学意义(P=0.793,P=0.885)。结论在不孕症的病因诊断中,3D-Hy CoSy对输卵管通畅程度的诊断价值优于HSG。展开更多
The polycystic ovary syndrome (PCOS) model was established in fats and correlation between the expression of macrophage migration inhibitory factor (MIF) and cytokinesis with the MAPK signalling pathway in the rat ova...The polycystic ovary syndrome (PCOS) model was established in fats and correlation between the expression of macrophage migration inhibitory factor (MIF) and cytokinesis with the MAPK signalling pathway in the rat ovary was measured. The PCOS model in rats was established by dehydroepiandrosterone (DHEA).Thirty sexually immature female Sprague-Dawley rats were randomly and equally assigned to three groups:control group,PCOS group,and PCOS with high-fat diet (HFD) group.Serum hormones were assayed by radioimmunoassay (RIA).The ovaries'were immunohistochemically stained with MIF,and the expression of MIF,p-JNK and p-p38 was detected by Western blotting in ovaries.The serum testosterone level,LH concentration,LH/FSH ratio,fasting insulin level and HOMA IR index in the PCOS group (6.077±0.478,13.809±1.701,1.820±0.404,10.83±1.123 and 1.8692±0.1096)and PCOS with HFD group (6.075±0.439,14.075±1.927,1.779±0.277,10.20±1.377 and 1.7736±0.6851)were significantly higher than those in the control group (4.949±0.337, 2.458±0.509,1.239±0.038,9.53±0.548 and 1.5329±0.7363),but there was no significant difference between the PCOS group and PCOS with HFD group.The expression levels of MIF,p-JNK,and p-p38 in the PCOS group (0.4048±0.013,0.6233±0.093 and 0.7987±0.061)and PCOS withHFD group (0.1929±0.012,0.3346±0.103 and 0.3468±0.031)were obviously higher than those in control group (0.2492±0.013, 0.3271±0.093 and 0.3393±0.061),but no Significant difference was observed between PCOS group and PCOS with HFD group.It was suggested that MIF may participate in the pathogenesis of PCOS through the MAPK signalling pathway in PCOS rats induced by DHEA.展开更多
文摘目的探讨经阴道子宫输卵管实时三维超声造影(RT-3D-Hy Co Sy)和X线碘水造影(HSG)在不孕症临床诊治中的应用价值。方法回顾性分析2017年5~11月在云浮市人民医院就诊的不孕症患者的临床资料,以腹腔镜输卵管通液术(LTF)为金标准,比较RT-3D-Hy CoSy和HSG评估输卵管通畅程度和输卵管有无积水的准确率,分析两种技术的临床应用价值。结果 121例患者进行3D-Hy Co Sy检查,170例患者进行HSG检查,38例患者进行腹腔镜输卵管通液术治疗。对输卵管通畅程度的评估,3D-Hy CoSy、HSG与LTF的总符合率分别为85.5%(κ=0.735)和78.9%(κ=0.652);对输卵管有无积水的评估,3D-Hy CoSy、HSG与LTF的总符合率分别为81.6%(κ=0.685)和80.3%(κ=0.643)。两种技术诊断输卵管通畅程度和有无积水的准确率差异均无统计学意义(P=0.793,P=0.885)。结论在不孕症的病因诊断中,3D-Hy CoSy对输卵管通畅程度的诊断价值优于HSG。
基金This project was in part supported by the National Natural Science Foundation of China (No.30973196).
文摘The polycystic ovary syndrome (PCOS) model was established in fats and correlation between the expression of macrophage migration inhibitory factor (MIF) and cytokinesis with the MAPK signalling pathway in the rat ovary was measured. The PCOS model in rats was established by dehydroepiandrosterone (DHEA).Thirty sexually immature female Sprague-Dawley rats were randomly and equally assigned to three groups:control group,PCOS group,and PCOS with high-fat diet (HFD) group.Serum hormones were assayed by radioimmunoassay (RIA).The ovaries'were immunohistochemically stained with MIF,and the expression of MIF,p-JNK and p-p38 was detected by Western blotting in ovaries.The serum testosterone level,LH concentration,LH/FSH ratio,fasting insulin level and HOMA IR index in the PCOS group (6.077±0.478,13.809±1.701,1.820±0.404,10.83±1.123 and 1.8692±0.1096)and PCOS with HFD group (6.075±0.439,14.075±1.927,1.779±0.277,10.20±1.377 and 1.7736±0.6851)were significantly higher than those in the control group (4.949±0.337, 2.458±0.509,1.239±0.038,9.53±0.548 and 1.5329±0.7363),but there was no significant difference between the PCOS group and PCOS with HFD group.The expression levels of MIF,p-JNK,and p-p38 in the PCOS group (0.4048±0.013,0.6233±0.093 and 0.7987±0.061)and PCOS withHFD group (0.1929±0.012,0.3346±0.103 and 0.3468±0.031)were obviously higher than those in control group (0.2492±0.013, 0.3271±0.093 and 0.3393±0.061),but no Significant difference was observed between PCOS group and PCOS with HFD group.It was suggested that MIF may participate in the pathogenesis of PCOS through the MAPK signalling pathway in PCOS rats induced by DHEA.
