OBJECTIVE Right ventricular(RV)remodeling is one of the essential pathological features in pulmonary arterial hypertension(PAH).RV hypertrophy or fibrosis are the leading causes of RV remodeling.Magnolol is a compound...OBJECTIVE Right ventricular(RV)remodeling is one of the essential pathological features in pulmonary arterial hypertension(PAH).RV hypertrophy or fibrosis are the leading causes of RV remodeling.Magnolol is a compound isolated from Magnolia officinalis.It possesses multiple pharmacological activities,such as anti-oxidation and anti-inflammation.This study aims to evaluate the effects and underlying mechanisms of magnolol on RV remodeling in hypoxia-induced PAH.METHODS①Male SD rats(220 g)were randomly divided into 5 groups(n=10):the normoxia group,the hypoxia group,the hypoxia plus Magnolol(10 and 20 mg·kg^(-1)·d-1)group,and the vehicle group.Rats in the normoxia group were kept in a normoxia environment for 4 weeks,while rats in the hypoxia group were kept in a hypoxic chamber(10%O2).The rats in the hypoxia plus magnolol groups were administered with magnolol at 10 or 20 mg·kg^(-1)(ip)once a day for 4 weeks.At the end of 4 weeks,the heart function was assessed by Doppler echocardiography,and then the rats were anesthetized with sodium pentobarbital(30 mg·kg^(-1),ip).The RVSP was measured by the right heart catheterization method.The heart tissues were collected and dissected to calculate the index of RV remodeling(RV/LV+IVS,RV/tibial length,or RV/body weight).Part of the RV samples was fixed with 4%paraformaldehyde for morphological analysis,while other samples were frozen at-80℃for molecular studies(measurements of ANP,BNP,α-SMA,and collagenⅠ/ⅢmRNA expression as well as p-JAK2/JAK2 and p-STAT3/STAT3 protein levels).②To evaluate the effect of magnolol on hypoxia-induced myocardial hypertrophy and fibrosis,H9c2 or cardiac fibroblasts were divided into 7 groups:the control group,cells were cultured under normal conditions;the hypoxia group,cells were cultured under hypoxic condition(3%O2);the hypoxia plus magnolol 10 mg·kg^(-1) group,magnolol10μmol·L^(-1) was added to the culture medium before the hypoxia treatment;the hypoxia plus magnolol 30 mg·kg^(-1) group,magnolol 20μmol·L^(-1) was added t展开更多
High concentrations of antimony(Sb) in soils and vegetables can cause potential health risk. However, the effect of Sb on the growth and response of crops are not well known and to date, there is still no Sb limit sta...High concentrations of antimony(Sb) in soils and vegetables can cause potential health risk. However, the effect of Sb on the growth and response of crops are not well known and to date, there is still no Sb limit standard for Allitic Udic Ferrisols in China. In this study, a greenhouse experiment was carried out to investigate the effect of antimony(Sb) on biomass, physiological performances,and macro- and micronutrient element concentrations of green Chinese cabbage(Brassica chinensis L.), as well as enzyme activities,in Allitic Udic Ferrisols from Hunan Province, China. Antimony was supplied at rates of 0(control), 2, 5, 10, 20, and 50 mg kg-1and thus with the background value of 1.0 mg kg-1, the Sb concentrations in the treated soil samples were 1, 3, 6, 11 21, and 51 mg kg-1, respectively. The results showed the leaf biomass and ascorbic acid content of cabbage significantly(P < 0.05) decreased by 30.6% and 48.3%, respectively, and soil urease and dehydrogenase activities also significantly(P < 0.05) decreased by 33.6%and 32.5%, respectively, when soil Sb concentration was 21 mg kg-1as compared with the control. The uptake of essential nutrient elements such as Mg, Cu, and Zn by cabbage was obviously affected, while the leaf soluble sugar content slightly changed when the soil Sb concentration exceeded 21 mg kg-1. Based on cabbage physiological responses and soil enzyme activities, the permissible concentration of 21 mg kg-1for Sb in Allitic Udic Ferrisols should be recommended.展开更多
手术成为治疗结直肠恶性肿瘤的首选方法.自加速康复外科(enhanced recovery after surgery,ERAS)理论的主要内容之一就是强调肠内营养在结直肠恶性肿瘤患者术后的恢复中起到重要的作用,而肠道免疫微生态营养在肠内营养的过程中扮演着重...手术成为治疗结直肠恶性肿瘤的首选方法.自加速康复外科(enhanced recovery after surgery,ERAS)理论的主要内容之一就是强调肠内营养在结直肠恶性肿瘤患者术后的恢复中起到重要的作用,而肠道免疫微生态营养在肠内营养的过程中扮演着重要的角色.本研究在加速康复外科理论基础上加用免疫微生态营养制剂,观察患者术后恢复情况及机体免疫状态、患者的预后,探讨免疫微生态制剂是否可以成为结直肠癌手术加速康复外科一种新的处理手段,从而推动加速康复外科的发展应用.展开更多
文摘OBJECTIVE Right ventricular(RV)remodeling is one of the essential pathological features in pulmonary arterial hypertension(PAH).RV hypertrophy or fibrosis are the leading causes of RV remodeling.Magnolol is a compound isolated from Magnolia officinalis.It possesses multiple pharmacological activities,such as anti-oxidation and anti-inflammation.This study aims to evaluate the effects and underlying mechanisms of magnolol on RV remodeling in hypoxia-induced PAH.METHODS①Male SD rats(220 g)were randomly divided into 5 groups(n=10):the normoxia group,the hypoxia group,the hypoxia plus Magnolol(10 and 20 mg·kg^(-1)·d-1)group,and the vehicle group.Rats in the normoxia group were kept in a normoxia environment for 4 weeks,while rats in the hypoxia group were kept in a hypoxic chamber(10%O2).The rats in the hypoxia plus magnolol groups were administered with magnolol at 10 or 20 mg·kg^(-1)(ip)once a day for 4 weeks.At the end of 4 weeks,the heart function was assessed by Doppler echocardiography,and then the rats were anesthetized with sodium pentobarbital(30 mg·kg^(-1),ip).The RVSP was measured by the right heart catheterization method.The heart tissues were collected and dissected to calculate the index of RV remodeling(RV/LV+IVS,RV/tibial length,or RV/body weight).Part of the RV samples was fixed with 4%paraformaldehyde for morphological analysis,while other samples were frozen at-80℃for molecular studies(measurements of ANP,BNP,α-SMA,and collagenⅠ/ⅢmRNA expression as well as p-JAK2/JAK2 and p-STAT3/STAT3 protein levels).②To evaluate the effect of magnolol on hypoxia-induced myocardial hypertrophy and fibrosis,H9c2 or cardiac fibroblasts were divided into 7 groups:the control group,cells were cultured under normal conditions;the hypoxia group,cells were cultured under hypoxic condition(3%O2);the hypoxia plus magnolol 10 mg·kg^(-1) group,magnolol10μmol·L^(-1) was added to the culture medium before the hypoxia treatment;the hypoxia plus magnolol 30 mg·kg^(-1) group,magnolol 20μmol·L^(-1) was added t
基金supported by the National Natural Science Foundation of China(No.41201492)the Science and Technology Project of Changsha City,China(No.K1003056-31)
文摘High concentrations of antimony(Sb) in soils and vegetables can cause potential health risk. However, the effect of Sb on the growth and response of crops are not well known and to date, there is still no Sb limit standard for Allitic Udic Ferrisols in China. In this study, a greenhouse experiment was carried out to investigate the effect of antimony(Sb) on biomass, physiological performances,and macro- and micronutrient element concentrations of green Chinese cabbage(Brassica chinensis L.), as well as enzyme activities,in Allitic Udic Ferrisols from Hunan Province, China. Antimony was supplied at rates of 0(control), 2, 5, 10, 20, and 50 mg kg-1and thus with the background value of 1.0 mg kg-1, the Sb concentrations in the treated soil samples were 1, 3, 6, 11 21, and 51 mg kg-1, respectively. The results showed the leaf biomass and ascorbic acid content of cabbage significantly(P < 0.05) decreased by 30.6% and 48.3%, respectively, and soil urease and dehydrogenase activities also significantly(P < 0.05) decreased by 33.6%and 32.5%, respectively, when soil Sb concentration was 21 mg kg-1as compared with the control. The uptake of essential nutrient elements such as Mg, Cu, and Zn by cabbage was obviously affected, while the leaf soluble sugar content slightly changed when the soil Sb concentration exceeded 21 mg kg-1. Based on cabbage physiological responses and soil enzyme activities, the permissible concentration of 21 mg kg-1for Sb in Allitic Udic Ferrisols should be recommended.
文摘手术成为治疗结直肠恶性肿瘤的首选方法.自加速康复外科(enhanced recovery after surgery,ERAS)理论的主要内容之一就是强调肠内营养在结直肠恶性肿瘤患者术后的恢复中起到重要的作用,而肠道免疫微生态营养在肠内营养的过程中扮演着重要的角色.本研究在加速康复外科理论基础上加用免疫微生态营养制剂,观察患者术后恢复情况及机体免疫状态、患者的预后,探讨免疫微生态制剂是否可以成为结直肠癌手术加速康复外科一种新的处理手段,从而推动加速康复外科的发展应用.
