Background CecropinXJ belongs to cecropinB, which is the most potent antibacterial peptide found naturally The aim of this study was to investigate the effects of cecropinXJ on growth and adherence of oral cariogenic ...Background CecropinXJ belongs to cecropinB, which is the most potent antibacterial peptide found naturally The aim of this study was to investigate the effects of cecropinXJ on growth and adherence of oral cariogenic bacteriaMethods Four oral cariogenic bacteria (Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus and Actinomyces naeslundii) were chosen for this experiment The minimal inhibitory concentrations (MICs) and reductive percent of bacterial growth were used to assay the antibacterial activity of cecropinXJ Mammalian cytotoxicity of cecropinXJ was tested with human periodontal membrane fibroblasts by tetrazolium (MTT) colorimetric assay The bacterial morphological changes induced by cecropinXJ were examined on scanning electron microscope (SEM) The influence of cecropinXJ on bacterial adhesion to salivacoated hydroxyapatite (SHA) was measured by scintillation countingResults The MICs of cecropinXJ for inhibition of the growth of four bacteria ranged from 40 to 428 μmol/L with the highest susceptible to A naeslundii and the lowest susceptible to L acidophilus At pH 68, 55 and 82, 1/2 MIC of cecropinXJ reduced the number of viable bacteria by 409%, 678% and 328% for S mutans and by 281%, 572% and 379% for L acidophilus The activities against Smutans and L acidophilus increased at pH 55 compared with pH 68 (P<001, respectively) In present of 50% saliva, 1/2 MIC of the peptide decreased the direct count of viable cells by 292% and 144% for S mutans and L acidophilus, respectively (P<001 and P>005, respectively), whereas almost no reduction counts were detected in the presence of 20% serum for both bacteria (P>005, respectively) Mammalian cytotoxicity of cecropinXJ from 10 to 100 μmol/L exhibited no cytotoxicity against human periodontal membrane fibroblasts (P>005) Bacterial morphological changes induced by MIC of cecropinXJ examined on SEM showed cell surface disruption Furthermore, the ability of A naeslundii adhesion to SHA decreased significantly with MIC of cecropinXJ for 10 and 20展开更多
文摘Background CecropinXJ belongs to cecropinB, which is the most potent antibacterial peptide found naturally The aim of this study was to investigate the effects of cecropinXJ on growth and adherence of oral cariogenic bacteriaMethods Four oral cariogenic bacteria (Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus and Actinomyces naeslundii) were chosen for this experiment The minimal inhibitory concentrations (MICs) and reductive percent of bacterial growth were used to assay the antibacterial activity of cecropinXJ Mammalian cytotoxicity of cecropinXJ was tested with human periodontal membrane fibroblasts by tetrazolium (MTT) colorimetric assay The bacterial morphological changes induced by cecropinXJ were examined on scanning electron microscope (SEM) The influence of cecropinXJ on bacterial adhesion to salivacoated hydroxyapatite (SHA) was measured by scintillation countingResults The MICs of cecropinXJ for inhibition of the growth of four bacteria ranged from 40 to 428 μmol/L with the highest susceptible to A naeslundii and the lowest susceptible to L acidophilus At pH 68, 55 and 82, 1/2 MIC of cecropinXJ reduced the number of viable bacteria by 409%, 678% and 328% for S mutans and by 281%, 572% and 379% for L acidophilus The activities against Smutans and L acidophilus increased at pH 55 compared with pH 68 (P<001, respectively) In present of 50% saliva, 1/2 MIC of the peptide decreased the direct count of viable cells by 292% and 144% for S mutans and L acidophilus, respectively (P<001 and P>005, respectively), whereas almost no reduction counts were detected in the presence of 20% serum for both bacteria (P>005, respectively) Mammalian cytotoxicity of cecropinXJ from 10 to 100 μmol/L exhibited no cytotoxicity against human periodontal membrane fibroblasts (P>005) Bacterial morphological changes induced by MIC of cecropinXJ examined on SEM showed cell surface disruption Furthermore, the ability of A naeslundii adhesion to SHA decreased significantly with MIC of cecropinXJ for 10 and 20
