An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromy...An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other bi- nary vector p13W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing p13W4 and p13W8 mixed at a ratio of 1︰9, were used to transform a high-yield rice strain Chao 2-10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1seeds with GUS positive were eliminated. The GUS nega- tive T1 seeds were germinated, and the seedlings were ana- lyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were con- firmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice re- source for eating quality improvement.展开更多
During spaceflight,the cardiovascular system undergoes remarkable adaptation to microgravity and faces the risk of cardiac remodeling.Therefore,the effects and mechanisms of microgravity on cardiac morphology,physiolo...During spaceflight,the cardiovascular system undergoes remarkable adaptation to microgravity and faces the risk of cardiac remodeling.Therefore,the effects and mechanisms of microgravity on cardiac morphology,physiology,metabolism,and cellular biology need to be further investigated.Since China started constructing the China Space Station(CSS)in 2021,we have taken advantage of the Shenzhou-13 capsule to send human pluripotent stem cell-derived cardiomyocytes(hPSC-CMs)to the Tianhe core module of the CSS.In this study,hPSC-CMs subjected to space microgravity showed decreased beating rate and abnormal intracellular calcium cycling.Metabolomic and transcriptomic analyses revealed a battery of metabolic remodeling of hPSC-CMs in spaceflight,especially thiamine metabolism.The microgravity condition blocked the thiamine intake in hPSC-CMs.The decline of thiamine utilization under microgravity or by its antagonistic analog amprolium affected the process of the tricarboxylic acid cycle.It decreased ATP production,which led to cytoskeletal remodeling and calcium homeostasis imbalance in hPSC-CMs.More importantly,in vitro and in vivo studies suggest that thiamine supplementation could reverse the adaptive changes induced by simulated microgravity.This study represents the first astrobiological study on the China Space Station and lays a solid foundation for further aerospace biomedical research.These data indicate that intervention of thiamine-modified metabolic reprogramming in human cardiomyocytes during spaceflight might be a feasible countermeasure against microgravity.展开更多
The influences of the lateral motion of a single wheelset running on a tangent railway on the creepages and creep forces between wheel and rail are investigated with numerical methods. ...The influences of the lateral motion of a single wheelset running on a tangent railway on the creepages and creep forces between wheel and rail are investigated with numerical methods. The effect of the yaw motion of wheelset is neglected in the analysis, and Kalker’s theory of three dimensional elastic bodies in rolling contact is employed to analyze the creep forces in the wheel/rail rolling contact with Non Hertzian form.展开更多
Polymer nanowire(NW)organic field-effect transistors(OFETs)integrated on highly aligned large-area flexible substrates are candidate structures for the development of high-performance flexible electronics.This work pr...Polymer nanowire(NW)organic field-effect transistors(OFETs)integrated on highly aligned large-area flexible substrates are candidate structures for the development of high-performance flexible electronics.This work presents a universal technique,coaxial focused electrohydrodynamic jet(CFEJ)printing technology,to fabricate highly aligned 90-nm-diameter polymer arrays.This method allows for the preparation of uniformly shaped and precisely positioned nanowires directly on flexible substrates without transfer,thus ensuring their electrical properties.Using indacenodithiophene-co-benzothiadiazole(IDT-BT)and poly(9,9-dioctylfluorene-co-benzothiadiazole)(F8-BT)as example materials,5 cm^(2) arrays were prepared with only minute size variations,which is extremely difficult to do using previously reported methods.According to 2D-GIXRD analysis,the molecules inside the nanowires mainly adopted face-onπ-stacking crystallite arrangements.This is quite different from the mixed arrangement of thin films.Nanowire-based OFETs exhibited a high average hole mobility of 1.1 cm^(2) V^(−1) s^(−1) and good device uniformity,indicating the applicability of CFEJ printing as a potential batch manufacturing and integration process for high-performance,scalable polymer nanowire-based OFET circuits.This technique can be used to fabricate various polymer arrays,enabling the use of organic polymer semiconductors in large-area,high-performance electronic devices and providing a new path for the fabrication of flexible displays and wearable electronics in the future.展开更多
Traffic congestion is a growing problem in urban areas all over the world. The transport sector has been in full swing event study on intelligent transportation system for automatic detection. The functionality of aut...Traffic congestion is a growing problem in urban areas all over the world. The transport sector has been in full swing event study on intelligent transportation system for automatic detection. The functionality of automatic incident detection on expressways is a primary objective of advanced traffic management system. In order to save lives and prevent secondary incidents, accurate and prompt incident detection is necessary. This paper presents a methodology that integrates moving average （MA） model with stationary wavelet decomposition for automatic incident detection, in which parameters of layer coefficient are extracted from the difference between the upstream and downstream occupancy. Unlike other wavelet-based method presented before, firstly it smooths the raw data with MA model. Then it uses stationary wavelet to decompose, which can achieve accurate reconstruction of the signal, and does not shift the signal transfer coefficients. Thus, it can detect the incidents more accurately. The threshold to trigger incident alarm is also adjusted according to normal traffic condition with con- gestion. The methodology is validated with real data from Tokyo Expressway ultrasonic sensors. Ex- perimental results show that it is accurate and effective, and that it can differentiate traffic accident from other condition such as recurring traffic congestion.展开更多
目的:探讨下调生长阻滞和DNA损伤诱导蛋白45β(growth arrest and DNA damage inducible protein 45β,GADD45β)表达对PC9肺腺癌细胞及吉非替尼敏感性的影响。方法:设计并合成GADD45β基因小干扰RNA(GADD45β-small interfering RNA,GA...目的:探讨下调生长阻滞和DNA损伤诱导蛋白45β(growth arrest and DNA damage inducible protein 45β,GADD45β)表达对PC9肺腺癌细胞及吉非替尼敏感性的影响。方法:设计并合成GADD45β基因小干扰RNA(GADD45β-small interfering RNA,GADD45β-siRNA)序列,通过慢病毒介导将GADD45β-siRNA转入PC9肺腺癌细胞中,通过实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)和Western印迹检测转染前后PC9肺腺癌细胞GADD45β的mRNA及蛋白水平,采用膜联蛋白V(annexin V)-别藻蓝蛋白(allophycocyanin,APC)双染流式细胞法检测转染后细胞凋亡水平;通过流式细胞术检测转染后细胞内DNA含量,计算转染后细胞各周期时相百分率,分析转染对细胞生长周期的影响;通过计数克隆形成数检测RNA干扰对细胞成瘤能力的影响;采用MTT法检测PC9肺腺癌细胞的吉非替尼半数抑制浓度IC50。结果:筛选出5'-AAATCCACTTCACGCTCAT-3'为GADD45β基因RNA干扰的有效序列。转染GADD45β-siRNA 48 h后,qRT-PCR和Western印迹结果显示PC9肺腺癌细胞GADD45β的mRNA和蛋白表达水平明显下调(均P<0.05),细胞凋亡率明显增加(P<0.05),且成瘤克隆数明显减少(P<0.05);PC9肺腺癌细胞位于S期及G2/M期细胞增多(P<0.05),吉非替尼的IC50明显下降(P<0.05)。结论:PC9肺腺癌细胞转染GADD45β-siRNA后,能成功下调GADD45β基因的mRNA和蛋白表达;下调GADD45β表达可降低PC9肺腺癌细胞的克隆形成能力,促进细胞凋亡;下调GADD45β表达可明显提高PC9肺腺癌细胞对吉非替尼的敏感性。展开更多
In 2021,China Space Station(CSS)was assembled and constructed in-orbit,which provided a broad space platform for space medicine and space life sciences research.Space medicine focuses on the systematic risks that rest...In 2021,China Space Station(CSS)was assembled and constructed in-orbit,which provided a broad space platform for space medicine and space life sciences research.Space medicine focuses on the systematic risks that restrict long-term manned spaceflight.It carries out systematic research on the impact and countermeasure technology of long-term weightlessness on astronauts’health,space radiation on astronauts’health,behavior and ability,advanced on-orbit monitoring and medical disposal technology,and traditional space medical application technology by the space medicine experiment platform on human body and cell.It has accumulated valuable data on space environmental effects and human factors research,established a“human system risk”research system,obtained new knowledge and discoveries of space medicine,and explored countermeasure technologies of new characteristics.Moreover,a series of achievements have been made in the basic research on the mechanism of the special space environmental effect,which provides a solid foundation for the implementation of space missions.展开更多
Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory du...Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.展开更多
With strong alkaline anion-exchange resin 717 as the sorbent and NaOH solution as the eluent, a study on the sorption from alkaline solution and elution of vanadium（V）, silicon（iv）, and aluminium（III） was carrie...With strong alkaline anion-exchange resin 717 as the sorbent and NaOH solution as the eluent, a study on the sorption from alkaline solution and elution of vanadium（V）, silicon（iv）, and aluminium（III） was carried out. Different parameters affecting the sorption and elution process, including temperature, pH values as well as the ratio of resin to solution, were investigated. The results show that sorption degree of vanadium（V） increases with a decrease ofpH values, and V（V） ions are easier sorbed than Si（IV） and AI（III） ions under the same conditions. The sorption degree ofV（V）, Si（IV）, and AI（III） at pH 9.14 for 15 man are 90.6%, 33.5%, and 21.6%, respectively. Si（IV）, AI（III）, and V（V） ions sorbed on 717 resin were eluted by use of 2 mol·L^-1 NaOH solution; the desorption degree ofV（V）, Si（IV）, and AI（III） for 5 min are 81.7 %, 99.1%, and 99.3%, respectively.展开更多
In multi-cell cooperative multi-input multi-output （MIMO） systems, base station （BS） can exchange and utilize channel state information （CSI） of adjacent cell users to manage co-channel interference. Users quant...In multi-cell cooperative multi-input multi-output （MIMO） systems, base station （BS） can exchange and utilize channel state information （CSI） of adjacent cell users to manage co-channel interference. Users quantize the CSIs of desired channel and interference channels using finite-rate feedback links, then BS can generate cooperative block diagonalization （BD） precoding matrices using the obtained quantized CSI at transmitter to supress co-channel interference. In this paper, a novel adaptive bit allocation scheme is proposed to minimize the rate loss due to imperfect CSI. We derive the closed-form expression of rate loss caused by both channel delay and limited feedback. Based on the derived rate loss expression, the proposed scheme can adaptively allocate more bits to quantize the better channels with smaller delays and fewer bits to worse channels with larger delays. Simulation results show that the proposed scheme yields higher performance than other allocation schemes.展开更多
In this paper, by using the theory of semigroup and spectrum, a computation formula on the growth order of one class of C0-semigroups in Banach space isproved.
基金the Shanghai Science and Technology Development Fund(Grant No.023112027).
文摘An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other bi- nary vector p13W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing p13W4 and p13W8 mixed at a ratio of 1︰9, were used to transform a high-yield rice strain Chao 2-10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1seeds with GUS positive were eliminated. The GUS nega- tive T1 seeds were germinated, and the seedlings were ana- lyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were con- firmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice re- source for eating quality improvement.
基金This study was supported by the National Key R&D Program of China(2022YFA1104300)the Space Medical Experiment Project of China Manned Space Program(HYZHXM01018)+4 种基金the National Natural Science Foundation of China(82241202)Scientific Innovation Project of the State Key Laboratory of Radiation Medicine and Protection(GZC00101)The Natural Science Foundation of the Jiangsu Higher Education Institutions of China(21KJB310003)Jiangsu Cardiovascular Medicine Innovation Center(CXZX202210)National Center for International Research(2017B01012).
文摘During spaceflight,the cardiovascular system undergoes remarkable adaptation to microgravity and faces the risk of cardiac remodeling.Therefore,the effects and mechanisms of microgravity on cardiac morphology,physiology,metabolism,and cellular biology need to be further investigated.Since China started constructing the China Space Station(CSS)in 2021,we have taken advantage of the Shenzhou-13 capsule to send human pluripotent stem cell-derived cardiomyocytes(hPSC-CMs)to the Tianhe core module of the CSS.In this study,hPSC-CMs subjected to space microgravity showed decreased beating rate and abnormal intracellular calcium cycling.Metabolomic and transcriptomic analyses revealed a battery of metabolic remodeling of hPSC-CMs in spaceflight,especially thiamine metabolism.The microgravity condition blocked the thiamine intake in hPSC-CMs.The decline of thiamine utilization under microgravity or by its antagonistic analog amprolium affected the process of the tricarboxylic acid cycle.It decreased ATP production,which led to cytoskeletal remodeling and calcium homeostasis imbalance in hPSC-CMs.More importantly,in vitro and in vivo studies suggest that thiamine supplementation could reverse the adaptive changes induced by simulated microgravity.This study represents the first astrobiological study on the China Space Station and lays a solid foundation for further aerospace biomedical research.These data indicate that intervention of thiamine-modified metabolic reprogramming in human cardiomyocytes during spaceflight might be a feasible countermeasure against microgravity.
文摘The influences of the lateral motion of a single wheelset running on a tangent railway on the creepages and creep forces between wheel and rail are investigated with numerical methods. The effect of the yaw motion of wheelset is neglected in the analysis, and Kalker’s theory of three dimensional elastic bodies in rolling contact is employed to analyze the creep forces in the wheel/rail rolling contact with Non Hertzian form.
基金This research was supported by the National Key R&D Program of China(Grant No.2018YFA0703200,D.W.)National Natural Science Foundation of China(51975104 and 62074138,D.W.52003274,Z.Z.)+2 种基金the Fundamental Research Funds for the Central Universities(DUT22LAB405,DUT22QN227)Defense Industrial Technology Development Program(JCKY2020204B004)Ningbo Institute of Dalian University of Technology.The 2D-GIXRD data were obtained at 1W1A,Beijing Synchrotron Radiation Facility.The authors gratefully acknowledge the assistance of researchers of the Diffuse X-ray Scattering Station during the experiments.Special thanks are due to Dr.Cai Rui in the Instrumental Analysis Center of Dalian University of Technology for assistance with AFM analysis.
文摘Polymer nanowire(NW)organic field-effect transistors(OFETs)integrated on highly aligned large-area flexible substrates are candidate structures for the development of high-performance flexible electronics.This work presents a universal technique,coaxial focused electrohydrodynamic jet(CFEJ)printing technology,to fabricate highly aligned 90-nm-diameter polymer arrays.This method allows for the preparation of uniformly shaped and precisely positioned nanowires directly on flexible substrates without transfer,thus ensuring their electrical properties.Using indacenodithiophene-co-benzothiadiazole(IDT-BT)and poly(9,9-dioctylfluorene-co-benzothiadiazole)(F8-BT)as example materials,5 cm^(2) arrays were prepared with only minute size variations,which is extremely difficult to do using previously reported methods.According to 2D-GIXRD analysis,the molecules inside the nanowires mainly adopted face-onπ-stacking crystallite arrangements.This is quite different from the mixed arrangement of thin films.Nanowire-based OFETs exhibited a high average hole mobility of 1.1 cm^(2) V^(−1) s^(−1) and good device uniformity,indicating the applicability of CFEJ printing as a potential batch manufacturing and integration process for high-performance,scalable polymer nanowire-based OFET circuits.This technique can be used to fabricate various polymer arrays,enabling the use of organic polymer semiconductors in large-area,high-performance electronic devices and providing a new path for the fabrication of flexible displays and wearable electronics in the future.
基金supported by Jiangsu Provincial Government Scholarshipthe National Natural Science Foundation of China(No.51008143)
文摘Traffic congestion is a growing problem in urban areas all over the world. The transport sector has been in full swing event study on intelligent transportation system for automatic detection. The functionality of automatic incident detection on expressways is a primary objective of advanced traffic management system. In order to save lives and prevent secondary incidents, accurate and prompt incident detection is necessary. This paper presents a methodology that integrates moving average （MA） model with stationary wavelet decomposition for automatic incident detection, in which parameters of layer coefficient are extracted from the difference between the upstream and downstream occupancy. Unlike other wavelet-based method presented before, firstly it smooths the raw data with MA model. Then it uses stationary wavelet to decompose, which can achieve accurate reconstruction of the signal, and does not shift the signal transfer coefficients. Thus, it can detect the incidents more accurately. The threshold to trigger incident alarm is also adjusted according to normal traffic condition with con- gestion. The methodology is validated with real data from Tokyo Expressway ultrasonic sensors. Ex- perimental results show that it is accurate and effective, and that it can differentiate traffic accident from other condition such as recurring traffic congestion.
文摘目的:探讨下调生长阻滞和DNA损伤诱导蛋白45β(growth arrest and DNA damage inducible protein 45β,GADD45β)表达对PC9肺腺癌细胞及吉非替尼敏感性的影响。方法:设计并合成GADD45β基因小干扰RNA(GADD45β-small interfering RNA,GADD45β-siRNA)序列,通过慢病毒介导将GADD45β-siRNA转入PC9肺腺癌细胞中,通过实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)和Western印迹检测转染前后PC9肺腺癌细胞GADD45β的mRNA及蛋白水平,采用膜联蛋白V(annexin V)-别藻蓝蛋白(allophycocyanin,APC)双染流式细胞法检测转染后细胞凋亡水平;通过流式细胞术检测转染后细胞内DNA含量,计算转染后细胞各周期时相百分率,分析转染对细胞生长周期的影响;通过计数克隆形成数检测RNA干扰对细胞成瘤能力的影响;采用MTT法检测PC9肺腺癌细胞的吉非替尼半数抑制浓度IC50。结果:筛选出5'-AAATCCACTTCACGCTCAT-3'为GADD45β基因RNA干扰的有效序列。转染GADD45β-siRNA 48 h后,qRT-PCR和Western印迹结果显示PC9肺腺癌细胞GADD45β的mRNA和蛋白表达水平明显下调(均P<0.05),细胞凋亡率明显增加(P<0.05),且成瘤克隆数明显减少(P<0.05);PC9肺腺癌细胞位于S期及G2/M期细胞增多(P<0.05),吉非替尼的IC50明显下降(P<0.05)。结论:PC9肺腺癌细胞转染GADD45β-siRNA后,能成功下调GADD45β基因的mRNA和蛋白表达;下调GADD45β表达可降低PC9肺腺癌细胞的克隆形成能力,促进细胞凋亡;下调GADD45β表达可明显提高PC9肺腺癌细胞对吉非替尼的敏感性。
文摘In 2021,China Space Station(CSS)was assembled and constructed in-orbit,which provided a broad space platform for space medicine and space life sciences research.Space medicine focuses on the systematic risks that restrict long-term manned spaceflight.It carries out systematic research on the impact and countermeasure technology of long-term weightlessness on astronauts’health,space radiation on astronauts’health,behavior and ability,advanced on-orbit monitoring and medical disposal technology,and traditional space medical application technology by the space medicine experiment platform on human body and cell.It has accumulated valuable data on space environmental effects and human factors research,established a“human system risk”research system,obtained new knowledge and discoveries of space medicine,and explored countermeasure technologies of new characteristics.Moreover,a series of achievements have been made in the basic research on the mechanism of the special space environmental effect,which provides a solid foundation for the implementation of space missions.
基金supported by the National Development and Reform Commission of China(No.20111158)。
文摘Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.
基金supported by the Guangxi Natural Scientific Foundation of China(No.0832111)the Guangxi Large Apparatus Cooperation Community Net Foundation of China(No.695-2008-108)
文摘With strong alkaline anion-exchange resin 717 as the sorbent and NaOH solution as the eluent, a study on the sorption from alkaline solution and elution of vanadium（V）, silicon（iv）, and aluminium（III） was carried out. Different parameters affecting the sorption and elution process, including temperature, pH values as well as the ratio of resin to solution, were investigated. The results show that sorption degree of vanadium（V） increases with a decrease ofpH values, and V（V） ions are easier sorbed than Si（IV） and AI（III） ions under the same conditions. The sorption degree ofV（V）, Si（IV）, and AI（III） at pH 9.14 for 15 man are 90.6%, 33.5%, and 21.6%, respectively. Si（IV）, AI（III）, and V（V） ions sorbed on 717 resin were eluted by use of 2 mol·L^-1 NaOH solution; the desorption degree ofV（V）, Si（IV）, and AI（III） for 5 min are 81.7 %, 99.1%, and 99.3%, respectively.
基金supported by the Important National Science & Technology Specific Projects(2010ZX03005-001-0)the Hi-Tech Research and Development of China(2006AA01Z272)the New Century Excellent Talents in University(NCET):(NCET-11-0593)
文摘In multi-cell cooperative multi-input multi-output （MIMO） systems, base station （BS） can exchange and utilize channel state information （CSI） of adjacent cell users to manage co-channel interference. Users quantize the CSIs of desired channel and interference channels using finite-rate feedback links, then BS can generate cooperative block diagonalization （BD） precoding matrices using the obtained quantized CSI at transmitter to supress co-channel interference. In this paper, a novel adaptive bit allocation scheme is proposed to minimize the rate loss due to imperfect CSI. We derive the closed-form expression of rate loss caused by both channel delay and limited feedback. Based on the derived rate loss expression, the proposed scheme can adaptively allocate more bits to quantize the better channels with smaller delays and fewer bits to worse channels with larger delays. Simulation results show that the proposed scheme yields higher performance than other allocation schemes.
文摘In this paper, by using the theory of semigroup and spectrum, a computation formula on the growth order of one class of C0-semigroups in Banach space isproved.
