Deep-seated large-scale toppling failure presents unique challenges in the study of natural slope deformation process in mountainous regions.An active deep-seated toppling process was identified in the Erguxi slope lo...Deep-seated large-scale toppling failure presents unique challenges in the study of natural slope deformation process in mountainous regions.An active deep-seated toppling process was identified in the Erguxi slope located in southwest China,which affected a large area and damaged critical transportation infrastructure with the volume of the deforming rock mass exceeding 24×10~6 m^3.It poses significant risks to the downstream Shiziping Hydropower Station by damming the Zagunao River.Field investigation and monitoring results indicate that the deformation of the Erguxi slope is in the advanced stage of deep-seated toppling process,with the formation of a disturbed belt but no identifiable master failure surface.It was postulated that the alternating tensile and shear strength associated with the hard/soft laminated rock strata of metasandstone and phyllite layers preclude the development of either a tensile or shear failure surface,which resulted in the continuous deformation and displacement without a catastrophic mass movement.The slope movement is in close association with the unfavorable geological conditions of the study area in addition to the construction of transportation infrastructure and the increase of the reservoir level.On the basis of the mechanism and intensity of the ongoing toppling deformation,a qualitative grading system was proposed to describe the toppling process and toevaluate the slope stability.This paper summarized the field observation and monitoring data on the toppling deformation for better characterizing its effect on the stability of the Erguxi slope.The qualitative grading system intends to provide a basis for quantitative study of large-scale deep-seated toppling process in metamorphic rocks.展开更多
目的:观察苦参素(MAT)对实验性自身免疫性脑脊髓炎(EAE)小鼠脊髓中Th1/Th17细胞表达的影响,探讨MAT对EAE小鼠的防治机制。方法:用MOG35-55诱导制备EAE小鼠模型,40只C57BL/6小鼠随机分为EAE组及MAT组(MAT,200mg/kg),MAT组从免疫后第13天...目的:观察苦参素(MAT)对实验性自身免疫性脑脊髓炎(EAE)小鼠脊髓中Th1/Th17细胞表达的影响,探讨MAT对EAE小鼠的防治机制。方法:用MOG35-55诱导制备EAE小鼠模型,40只C57BL/6小鼠随机分为EAE组及MAT组(MAT,200mg/kg),MAT组从免疫后第13天开始连续腹腔注射给药至第22天,同时EAE组小鼠腹腔注射等量0.9%氯化钠溶液,观察记录小鼠体质量变化,神经功能学评分,苏木素-伊红(HE)和Luxol Fast Blue(LFB)髓鞘染色观察病理改变,免疫荧光双染检测CD4+IFN-γ+与CD4+IL-17+共表达情况,Western Blot法测定IL-12蛋白表达情况。结果:与EAE组小鼠比较,MAT组显著降低EAE小鼠的神经功能学评分(P<0.01),减轻EAE小鼠神经炎症浸润(P<0.01)和髓鞘脱失(P<0.01),下调脊髓中CD4+IFN-γ+(P<0.05)与CD4+IL-17+(P<0.05)的共表达,且两组CD4+IL-17+与CD4+IFN-γ+的共表达均成正相关(r=0.888~0.933,P<0.05),同时降低脊髓中IL-12的蛋白表达(P<0.05)。结论:MAT对EAE小鼠具有防治作用,可能与下调小鼠脊髓中Th1/Th17细胞的表达有关。展开更多
【目的】阐明不同基质种植下石斛根系内生菌菌群的差异性,为进一步研究内生菌组成差异对石斛药效成分积累的影响、优化种植条件提供参考依据。【方法】选取仿野生贴石种植于丹霞石块和火山石块的2年生石斛根系,进行16S rRNA V4区域和IT...【目的】阐明不同基质种植下石斛根系内生菌菌群的差异性,为进一步研究内生菌组成差异对石斛药效成分积累的影响、优化种植条件提供参考依据。【方法】选取仿野生贴石种植于丹霞石块和火山石块的2年生石斛根系,进行16S rRNA V4区域和ITS 2区域的高通量测序,分析2种基质种植下石斛根系内生细菌和内生真菌的次生代谢产物、群落组成结构、alpha多样性、beta多样性和功能预测。【结果】火山石与丹霞石的石斛根系次生代谢产物中倍半萜糖苷类物质Dendromoniliside C or isomer(288-p)和Dendromoniliside D or isomer(123-n)差异显著(P<0.05,下同)。群落结构分析显示,内生细菌和内生真菌的组成在门水平和属水平上均无明显差异,其中,内生细菌在门水平上均以变形菌门(Proteobacteria)相对丰度最为富集,在属水平上均以鞘脂单胞菌属(Sphingomonas)相对丰度最为富集;内生真菌在门水平上均以子囊菌门(Ascomycota)为绝对优势门,在属水平上均以暗球腔菌属(Phaeosphaeria)为优势菌群。主成分分析反映火山石与丹霞石样本组间差异明显,基于ANOSIM组间差异检验下的Unweighted UniFrac指数、Bray Curtis指数和Jaccard指数均进一步表明不同基质种植下石斛内生菌的组间差异远大于组内差异。功能预测结果显示,在2种基质下石斛根系中,内生菌真菌营养型以病理腐生营养型(Pathotroph-Saprotroph)和腐生营养型(Saprotroph)差异最显著,在主要功能通路中真菌寄生—植物病原—植物腐生菌功能群(Fungal Parasite-Plant PathogenPlant Saprotroph)和未定义腐生菌功能群(Undefined Saprotroph)差异显著,均为丹霞石显著高于火山石。【结论】2种基质种植环境显著影响石斛根系内生菌群的次级代谢产物、菌群相对丰度、优势菌群和群落功能。同时也提示,石斛根系内生真菌相较于内生细菌,对不同种植基质条件更敏感。展开更多
Background Arrhythmogenic right ventricular dysplasia/cardiomyopathy is an inherited cardiomyopathy.European Society of Cardiology was devised a new prediction model to estimate ventricular arrhythmias and guide decis...Background Arrhythmogenic right ventricular dysplasia/cardiomyopathy is an inherited cardiomyopathy.European Society of Cardiology was devised a new prediction model to estimate ventricular arrhythmias and guide decisions regarding primary prevention ICDs.This paper aimed to conduct external validation of European prediction model in the South China.展开更多
基金financially supported by the National Natural Science Foundation of China (Grant No.41572302 and Grant No.41130745)the Funds for Creative Research Groups of China (Grant No.41521002)the Open Research Fund from the State Key Laboratory of Geohazard Prevention and Geoenvironment Protection (Chengdu University of Technology) (Grant No.SKLGP2015K001)
文摘Deep-seated large-scale toppling failure presents unique challenges in the study of natural slope deformation process in mountainous regions.An active deep-seated toppling process was identified in the Erguxi slope located in southwest China,which affected a large area and damaged critical transportation infrastructure with the volume of the deforming rock mass exceeding 24×10~6 m^3.It poses significant risks to the downstream Shiziping Hydropower Station by damming the Zagunao River.Field investigation and monitoring results indicate that the deformation of the Erguxi slope is in the advanced stage of deep-seated toppling process,with the formation of a disturbed belt but no identifiable master failure surface.It was postulated that the alternating tensile and shear strength associated with the hard/soft laminated rock strata of metasandstone and phyllite layers preclude the development of either a tensile or shear failure surface,which resulted in the continuous deformation and displacement without a catastrophic mass movement.The slope movement is in close association with the unfavorable geological conditions of the study area in addition to the construction of transportation infrastructure and the increase of the reservoir level.On the basis of the mechanism and intensity of the ongoing toppling deformation,a qualitative grading system was proposed to describe the toppling process and toevaluate the slope stability.This paper summarized the field observation and monitoring data on the toppling deformation for better characterizing its effect on the stability of the Erguxi slope.The qualitative grading system intends to provide a basis for quantitative study of large-scale deep-seated toppling process in metamorphic rocks.
文摘目的:观察苦参素(MAT)对实验性自身免疫性脑脊髓炎(EAE)小鼠脊髓中Th1/Th17细胞表达的影响,探讨MAT对EAE小鼠的防治机制。方法:用MOG35-55诱导制备EAE小鼠模型,40只C57BL/6小鼠随机分为EAE组及MAT组(MAT,200mg/kg),MAT组从免疫后第13天开始连续腹腔注射给药至第22天,同时EAE组小鼠腹腔注射等量0.9%氯化钠溶液,观察记录小鼠体质量变化,神经功能学评分,苏木素-伊红(HE)和Luxol Fast Blue(LFB)髓鞘染色观察病理改变,免疫荧光双染检测CD4+IFN-γ+与CD4+IL-17+共表达情况,Western Blot法测定IL-12蛋白表达情况。结果:与EAE组小鼠比较,MAT组显著降低EAE小鼠的神经功能学评分(P<0.01),减轻EAE小鼠神经炎症浸润(P<0.01)和髓鞘脱失(P<0.01),下调脊髓中CD4+IFN-γ+(P<0.05)与CD4+IL-17+(P<0.05)的共表达,且两组CD4+IL-17+与CD4+IFN-γ+的共表达均成正相关(r=0.888~0.933,P<0.05),同时降低脊髓中IL-12的蛋白表达(P<0.05)。结论:MAT对EAE小鼠具有防治作用,可能与下调小鼠脊髓中Th1/Th17细胞的表达有关。
文摘【目的】阐明不同基质种植下石斛根系内生菌菌群的差异性,为进一步研究内生菌组成差异对石斛药效成分积累的影响、优化种植条件提供参考依据。【方法】选取仿野生贴石种植于丹霞石块和火山石块的2年生石斛根系,进行16S rRNA V4区域和ITS 2区域的高通量测序,分析2种基质种植下石斛根系内生细菌和内生真菌的次生代谢产物、群落组成结构、alpha多样性、beta多样性和功能预测。【结果】火山石与丹霞石的石斛根系次生代谢产物中倍半萜糖苷类物质Dendromoniliside C or isomer(288-p)和Dendromoniliside D or isomer(123-n)差异显著(P<0.05,下同)。群落结构分析显示,内生细菌和内生真菌的组成在门水平和属水平上均无明显差异,其中,内生细菌在门水平上均以变形菌门(Proteobacteria)相对丰度最为富集,在属水平上均以鞘脂单胞菌属(Sphingomonas)相对丰度最为富集;内生真菌在门水平上均以子囊菌门(Ascomycota)为绝对优势门,在属水平上均以暗球腔菌属(Phaeosphaeria)为优势菌群。主成分分析反映火山石与丹霞石样本组间差异明显,基于ANOSIM组间差异检验下的Unweighted UniFrac指数、Bray Curtis指数和Jaccard指数均进一步表明不同基质种植下石斛内生菌的组间差异远大于组内差异。功能预测结果显示,在2种基质下石斛根系中,内生菌真菌营养型以病理腐生营养型(Pathotroph-Saprotroph)和腐生营养型(Saprotroph)差异最显著,在主要功能通路中真菌寄生—植物病原—植物腐生菌功能群(Fungal Parasite-Plant PathogenPlant Saprotroph)和未定义腐生菌功能群(Undefined Saprotroph)差异显著,均为丹霞石显著高于火山石。【结论】2种基质种植环境显著影响石斛根系内生菌群的次级代谢产物、菌群相对丰度、优势菌群和群落功能。同时也提示,石斛根系内生真菌相较于内生细菌,对不同种植基质条件更敏感。
文摘Background Arrhythmogenic right ventricular dysplasia/cardiomyopathy is an inherited cardiomyopathy.European Society of Cardiology was devised a new prediction model to estimate ventricular arrhythmias and guide decisions regarding primary prevention ICDs.This paper aimed to conduct external validation of European prediction model in the South China.
