Abstract To overcome the influence of the nonlinear friction on the gimbaled servo-system of an inertial stabilized platforms （ISPs） with DC motor direct-drive, the methods of modeling and compensation of the nonlin...Abstract To overcome the influence of the nonlinear friction on the gimbaled servo-system of an inertial stabilized platforms （ISPs） with DC motor direct-drive, the methods of modeling and compensation of the nonlinear friction are proposed. Firstly, the inapplicability of LuGre model when trying to interpret the backward angular displacement in the prestiction regime is observed experimentally and the reason is deduced theoretically. Then, based on the dynamic model of direct-drive ISPs, a modified LuGre model is proposed to describe the characteristic of the friction in the prestiction regime. Furthermore, the state switch condition of the three friction regimes including presliding, gross sliding and prestiction is presented. Finally, a composite compensation controller including a nonlinear friction observer and a feedforward compensator based on the novel LuGre model is designed to restrain the nonlinear friction and to improve the control precision. Experimental results indicate that compared with those of the conventional proportion-integrationdifferentiation （PID） control method and the PID plus LuGre model-based friction compensation method, the dwell-time has decreased from 0.2 s to almost 0 s, the position error decreased to 86.7% and the peak-to-peak value of position error decreased to 80% after the novel compensation controller is added. It concludes that the composite compensation controller can greatly improve the control precision of the dynamic sealed ISPs.展开更多
The proteome of the Arabidopsis flower has not been extensively studied previously. Here, we report a proteomic analysis of the wild type Arabidopsis flower. Using both two-dimensional electrophoresis/mass spectromet...The proteome of the Arabidopsis flower has not been extensively studied previously. Here, we report a proteomic analysis of the wild type Arabidopsis flower. Using both two-dimensional electrophoresis/mass spectrometry （2-DGEIMS） and multi-dimensional protein identification technology （MudPIT） approaches, we identified 2 446 proteins. Although a single experiment or analysis uncovered only a subset of the proteins we identified, a combination of multiple experiments and analyses facilitated the detection of a greater number of proteins. When proteins are grouped according to RNA expression levels revealed by microarray experiments, we found that proteins encoded by genes with relatively high levels of expression were detected with greater frequencies. On the other hand, at the level of the individual gene/protein, there was not a good correlation between protein spot intensity and microarray values. We also obtained strong evidence for post-translational modification from 2-DGE and MudPIT data. We detected proteins that are annotated to function in protein synthesis, folding, modification, and degradation, as well as the presence of regulatory proteins such as transcription factors and protein kinases. Finally, sequence and evolutionary analysis of genes for active methyl group metabolisms suggests that these genes are highly conserved. Our results allow the formulation of hypotheses regarding post-translational regulation of proteins in the flower, providing new understanding about Arabidopsis flower development and physiology.展开更多
Superalloys feature multi-elements and complex elemental ranges,which makes the proper composition selection difficult.In fact,more strict composition standards generally apply in practical productions.The objective o...Superalloys feature multi-elements and complex elemental ranges,which makes the proper composition selection difficult.In fact,more strict composition standards generally apply in practical productions.The objective of this paper is to understand and eventually to renew the composition standard via example of the most common grade Inconel 718.We have recently shown that t he alloy chemistry originates from a nearest-neighbor cluster[center-shell]plus a few next-neighbor glue atoms,or expressed in cluster for-mula[center-shell](glue atoms).By grouping the elements into Ni=(Ni,Co,Cu,Fe),Cr=(Cr,Mn,Si,Mo),and Nb=(Nb,Al,Ti),it is found that the reported alloys fall within a narrow composition zone Ni_(11.0-13.0)-Cr_(3.5-4.5)-Nb_(1) confined by cluster formulas of 16 and 18 atoms.This composition zone is also expressed in terms of 288-atom supercluster formulas,Ni_(198-208)-Cr_(63-72)-Nb_(16-18),which leads to coordi-nated elemental variations in wt.%:69.0≤Ni+Co+Cu+Fe≤72.7;19.8≤Cr+Mn+1.7 Si+0.6 Mo≤22.8;8.7≤Nb+3.2 Al+1.9 Ti≤9.8.Within this composition zone,Ni_(206)-Cr_(65.5)-Nb_(16.5) is further pinpointed and validated by our own experiments to possess the optimal match of strength and plasticity both at room and at 923 K.展开更多
Objective： We aimed to evaluate the efficiency of serum testosterone suppression as well as the potential for agonistic stimulation of serum testosterone during chronic treatment with monthly （3.75 mg） depot formul...Objective： We aimed to evaluate the efficiency of serum testosterone suppression as well as the potential for agonistic stimulation of serum testosterone during chronic treatment with monthly （3.75 mg） depot formulation of domestic substitute of leuprorelin acetate microspheres for patients with metastatic prostate cancer. Methods： A total of 23 patients with metastatic prostate cancer were enrolled in the prospective study and received 6 monthly intramuscular depot injections of domestic substitute of leuprorelin acetate microspheres. Their levels and patterns of serum testosterone suppression and the potential for agonistic stimulation of serum testosterone were monitored following injection monthly （3.75 rag） depot formulation of domestic substitute of leuprorelin acetate microspheres for 24 weeks. Results： Mean testosterone was 431.4 ng/dL, 119.3 ng/dL, 28.2 ng/dL by week 1, 2, 3 and decreased to less than 15.6 ng/dL by week 4 where it remained throughout the treatment period. Median time to suppression of serum testosterone was 20.7 days. No transient minor ＂escape＂ from suppression occurred in all patients which was defined as a single testosterone value greater than 50 ng/dL once suppression was achieved. Assessment of agonistic stimulation following the second depot injection revealed no pattern of stimulation. Conclusion： We concluded that monthly （3.75 mg） depot formulation of domestic substitute of leuprorelin acetate microspheres could provide persistent, stable suppression of serum testosterone throughout the dosing intervals, and that the initial depot injection of this formulation also could provide sufficient pituitary desensitization to prevent agnostic stimulation of serum testosterone during chronic treatment.展开更多
A series of novel phenyl-urea derivatives which can simultaneously activate gluco kinase(GK)and peroxisome proliferator-activated receptorγ(PPARγ)was designed and prepared,and their activation of GK and PPARγ was e...A series of novel phenyl-urea derivatives which can simultaneously activate gluco kinase(GK)and peroxisome proliferator-activated receptorγ(PPARγ)was designed and prepared,and their activation of GK and PPARγ was evaluated.The structure--activity relationships of these compounds are also described.Three compounds showed potent ability to activate both GK and PPARγ.The possible binding mode of one of these compounds with GK and PPARγ were predicted by molecular docking simulation.展开更多
The absence of effective therapies for castration-resistant prostate cancer(CRPC) establishes the need to develop novel therapeutic modality, such as targeted gene therapy, which is ideal for the treatment of CRPC. Bu...The absence of effective therapies for castration-resistant prostate cancer(CRPC) establishes the need to develop novel therapeutic modality, such as targeted gene therapy, which is ideal for the treatment of CRPC. But its application has been limited due to lack of favorable gene vector and the reduction of "bystander effect". Consequently, scientists all over the world focus their main experimental research on the following four aspects: targeted gene, vector, transfer means and comprehensive therapy. In this paper, we reviewed the latest advances of experimental research on targeted gene therapy for prostate cancer.展开更多
Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive benefits.The objective of this study was to investigate the e...Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive benefits.The objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development,to determine the optimal DF intake for gilts,and gain microbial and metabolomic insight into the underlying mechanisms involved.Methods:Seventy-six Landrace×Yorkshire(LY)crossbred replacement gilts of similar age(92.6±0.6 d;mean±standard deviation[SD])and body weight(BW,33.8±3.9 kg;mean±SD)were randomly allocated to 4 dietary treatment groups(n=19);a basal diet without extra DF intake(DF 1.0),and 3 dietary groups ingesting an extra50%(DF 1.5),75%(DF 1.75),and 100%(DF 2.0)dietary fiber mixture consisting of inulin and cellulose(1:4).Oocyte maturation and uterine development were assessed on 19 d of the 2nd oestrous cycle.Microbial diversity of faecal samples was analysed by high-throughput pyrosequencing(16S r RNA)and blood samples were subjected to untargeted metabolomics.Results:The rates of oocytes showing first polar bodies after in vitro maturation for 44 h and uterine development increased linearly with increasing DF intake;DF 1.75 gilts had a 19.8%faster oocyte maturation rate and a 48.9 cm longer uterus than DF 1.0 gilts(P<0.05).Among the top 10 microbiota components at the phylum level,8increased linearly with increasing DF level,and the relative abundance of 30 of 53 microbiota components at the genus level(>0.1%)increased linearly or quadratically with increasing DF intake.Untargeted metabolic analysis revealed significant changes in serum metabolites that were closely associated with microbiota,including serotonin,a gut-derived signal that stimulates oocyte maturation.Conclusions:The findings provide evidence of the benefits of increased DF intake by supplementing inulin and cellulose on oocyte maturation and uterine development in gilts,and new microbial and metabolomic insight into the mechanisms mediating the ef展开更多
Thermodynamic properties for an alloy system play an important role in the materials science and engineer- ing. Therefore, theoretical calculations having the flexibility to deal with complexity are very useful and ha...Thermodynamic properties for an alloy system play an important role in the materials science and engineer- ing. Therefore, theoretical calculations having the flexibility to deal with complexity are very useful and have scien- tific meaning. The Hoch-Arpshofen model was deduced from physical principles and is applicable to binary, ternary and larger system using its binary interaction parameters only. Calculations of the activities of Fe-based liquid alloys are calculated using Hoch-Arpshofen model from data on the binary subsystems. Results for the activities for Fe-Au- Ni, Fe-Cr-Ni, Fe-Co-Cr and Fe-Co-Ni systems at required temperature are presented by Hoch-Arpshofen model. The average relative errors of prediction are 7.8%, 4.5%, 4.9~ and 2.7%, respectively. It shows that the calcu- lated results are in good agreement with the experimental data except Fe-Au-Ni system, which exhibits strong inter- action between unlike atoms. The model provides a simple, reliable and general method for calculating the activities for Fe-based liquid alloys.展开更多
Most of the XY positioning stages proposed in previous studies are mainly designed by considering only a single performance indicator of the stage. As a result, the other performance indicators are relatively weak. In...Most of the XY positioning stages proposed in previous studies are mainly designed by considering only a single performance indicator of the stage. As a result, the other performance indicators are relatively weak. In this study, a 2-degree-of-freedom linear compliant positioning stage （LCPS） is developed by mechatronic design to balance the interacting performance indicators and realize the desired positioning stage. The key parameters and the coupling of the structure and actuators are completely considered in the design. The LCPS consists of four voice coil motors （VCMs）, which are conformally designed for compactness, and six spatial leaf spring parallelograms. These parallelograms are serially connected for a large travel range and a high out-of-plane payload capacity. The mechatronic model is established by matrix structural analysis for structural modeling and by Kirchhof^s law for the VCMs. The sensitivities of the key parameters are analyzed, and the design parameters are subsequently determined. The analytical model of the stage is confirmed by experiments. The stage has a travel range of 4.4 mm 7.0 mm and a 0.16% area ratio of workspace to the outer dimension of the stage. The values of these performance indicators are greater than those of any existing stage reported in the literature. The closed-loop bandwidth is 9.5 Hz in both working directions. The stage can track a circular trajectory with a radius of 1.5 mm, with 40 Ixm error and a resolution of lower than 3 ~tm. The results of payload tests indicate that the stage has at least 20 kg out- of-plane payload capacity.展开更多
The increasing applications of seawater desalination technology have led to the wide usage of polyamide reverse osmosis membranes,resulting in a large number of wasted reverse osmosis membranes.In this work,the base n...The increasing applications of seawater desalination technology have led to the wide usage of polyamide reverse osmosis membranes,resulting in a large number of wasted reverse osmosis membranes.In this work,the base nonwoven layer of the wasted reverse osmosis membrane was successfully modified into the hydrophobic membrane via surface deposition strategy including TiO_(2) and 1H,1H,2H,2H-perfluorooctyltrichlorosilane(PFOTS),respectively.Various techniques were applied to characterize the obtained membranes,which were then used to separate the oil–water system.The optimally modified membrane displayed good hydrophobicity with a contact angle of 135.2°±0.3°,and its oil–water separation performance was as high as 97.8%.After 20 recycle tests,the oil–water separation performance remained more than 96%,which was attributed to the film adhesion of the anchored TiO_(2) and PFOTS layer on the surface.This work might provide a new avenue for recycling the wasted reverse osmosis membrane used in oily wastewater purification.展开更多
Objective Researchers have recently discovered that sublacustrine sedimentary exhalative mechanism associated with volcanism,is the principle way to form lacustrine exhalative rocks.These rocks differentiate themselve...Objective Researchers have recently discovered that sublacustrine sedimentary exhalative mechanism associated with volcanism,is the principle way to form lacustrine exhalative rocks.These rocks differentiate themselves from normal sedimentary rocks in their specificpetrofabric and material composition.展开更多
Using the survey data on 340 farmers in Chongqing City,this paper performs an empirical analysis of the factors influencing the non-point source pollution of pesticides. The results show that the older householders wi...Using the survey data on 340 farmers in Chongqing City,this paper performs an empirical analysis of the factors influencing the non-point source pollution of pesticides. The results show that the older householders will apply more pesticides,which may be due to the weak physical strength and weak ability to accept the concept of advanced cultivation; the householders with high level of education will choose to use less pesticides; the pesticide application rate is negatively correlated with whether farmers have participated in agricultural technology training,that is,the farmers having participated in agricultural technology training have stronger ability to scientifically apply pesticides,and in-depth understanding of advanced agricultural production technology and positive and negative effects of pesticides,so they often choose to reduce the application rate of pesticide; the cognitive factor on the role of pesticides in better promoting the growth of crops is significant,which requires the government and relevant departments to carry out concrete publicity of effectiveness and negative impact of different pesticides during the popularization of agricultural science knowledge,to prompt farmers to have a systematic and in-depth understanding of the agricultural nonpoint source pollution caused by pesticides.展开更多
In this work, surface-mazelike Zn O, Cu, and Ni hierarchical structures were synthesized via a versatile ethylene glycol- mediated solvothermal method. The structure evolution of these materials bore striking similari...In this work, surface-mazelike Zn O, Cu, and Ni hierarchical structures were synthesized via a versatile ethylene glycol- mediated solvothermal method. The structure evolution of these materials bore striking similarities, including(1) initial formation of metal alkoxides precursors and(2) subsequent structural evolution of products from tiered plates to jigsaw puzzles; then to extrusion ridges, nests and spindles; and thereafter to final mazelike structures driven by persistent thermal decomposition of preformed precursors. Based on their unique surface morphologies in sinuous asymmetry, it is anticipated that such mazelike hierarchical structures may shed new light on the development of morphology-controlled adsorption and heterogeneous catalysts.展开更多
Improper use of chemical fertilizers is an essential reason for diffused pollution of agriculture. Therefore,finding out influence factors of farmers in application of chemical fertilizers will play a significant role...Improper use of chemical fertilizers is an essential reason for diffused pollution of agriculture. Therefore,finding out influence factors of farmers in application of chemical fertilizers will play a significant role in controlling the diffused pollution of agriculture. Through field survey,a total of 340 samples in 4 counties of Chongqing Municipality were obtained. On the basis of these samples,an empirical study was carried out. The study results indicate that farmers' application of chemical fertilizers is negatively correlated with farmers' age,education level,male labor proportion,and soil fertility,while the annual family income,agricultural production population proportion,commodity trading characteristics,and scientific fertilizer application ability fail to pass the significance test. These results will provide reference for proper application of chemical fertilizers and controlling diffused pollution.展开更多
Two isostructural metal-organic frameworks formulated as [Gd7（L）4（μ3-OH）8（HCOO）（DMF）6（H20）].DMF·H2O （1） and [Tbv（L）4（P3-OH）8（HCOO）（DMF）5（HzO）]·DMFoH2O （2） （H3L=3,5-di（4＇-carboxylp...Two isostructural metal-organic frameworks formulated as [Gd7（L）4（μ3-OH）8（HCOO）（DMF）6（H20）].DMF·H2O （1） and [Tbv（L）4（P3-OH）8（HCOO）（DMF）5（HzO）]·DMFoH2O （2） （H3L=3,5-di（4＇-carboxylphenyl）benzoic acid） have been synthesized and structurally characterized. The building blocks of the structures are the heptanuclear lanthanide clusters of the dicubane-like type [Lnv7（μ3-OH）s]13＋ （Ln=Gd, Tb） which are formally assembled into porous three-dimensional networks through the linkage of L. Variable temperature magnetic studies down to 2 K reveal antiferromagnetic interactions in complex 1. Furthermore, the fluorescence property of 2 was also studied.展开更多
On the basis of the mild transformation of alkenyl triflates into alkynes promoted by LiCl, a one-pot protocol using alkenyl triflate precursors was developed for copper-mediated azide–alkyne cycloaddition. This prot...On the basis of the mild transformation of alkenyl triflates into alkynes promoted by LiCl, a one-pot protocol using alkenyl triflate precursors was developed for copper-mediated azide–alkyne cycloaddition. This protocol may provide an opportunity of sequentially click reactions for the construction of bifunctional probes in chemical biology studies.展开更多
In 2007,one living Ermia mangshanensis was found in the Shapingpian area at the junction of South China Tiger Nature Reserve in North Guangdong and Hunan Mangshan National Nature Reserve.To learn more about Ermia mang...In 2007,one living Ermia mangshanensis was found in the Shapingpian area at the junction of South China Tiger Nature Reserve in North Guangdong and Hunan Mangshan National Nature Reserve.To learn more about Ermia mangshanensis resource,the researchers in South China Tiger Nature Reserve in North Guangdong and Hunan Mangshan National Nature Reserve conducted field survey of Ermia mangshanensis in the Shapingpian area during the period 2010- 2012.The results show that there is Ermia mangshanensis in the Shapingpian area of South China Tiger Nature Reserve at the elevation of 800- 1200 m,and the population number is estimated at 48- 80.展开更多
基金co-supported by the National Natural Science Foundation of China (Nos. 51135009 and 51105371)
文摘Abstract To overcome the influence of the nonlinear friction on the gimbaled servo-system of an inertial stabilized platforms （ISPs） with DC motor direct-drive, the methods of modeling and compensation of the nonlinear friction are proposed. Firstly, the inapplicability of LuGre model when trying to interpret the backward angular displacement in the prestiction regime is observed experimentally and the reason is deduced theoretically. Then, based on the dynamic model of direct-drive ISPs, a modified LuGre model is proposed to describe the characteristic of the friction in the prestiction regime. Furthermore, the state switch condition of the three friction regimes including presliding, gross sliding and prestiction is presented. Finally, a composite compensation controller including a nonlinear friction observer and a feedforward compensator based on the novel LuGre model is designed to restrain the nonlinear friction and to improve the control precision. Experimental results indicate that compared with those of the conventional proportion-integrationdifferentiation （PID） control method and the PID plus LuGre model-based friction compensation method, the dwell-time has decreased from 0.2 s to almost 0 s, the position error decreased to 86.7% and the peak-to-peak value of position error decreased to 80% after the novel compensation controller is added. It concludes that the composite compensation controller can greatly improve the control precision of the dynamic sealed ISPs.
基金Supported by a Grant from the US Department of Energy (DE-FG02-02ER15332) to H. Maby funds from the Huck Institutes of the Life Sciences and the Biology Department at the Pennsylvania State University
文摘The proteome of the Arabidopsis flower has not been extensively studied previously. Here, we report a proteomic analysis of the wild type Arabidopsis flower. Using both two-dimensional electrophoresis/mass spectrometry （2-DGEIMS） and multi-dimensional protein identification technology （MudPIT） approaches, we identified 2 446 proteins. Although a single experiment or analysis uncovered only a subset of the proteins we identified, a combination of multiple experiments and analyses facilitated the detection of a greater number of proteins. When proteins are grouped according to RNA expression levels revealed by microarray experiments, we found that proteins encoded by genes with relatively high levels of expression were detected with greater frequencies. On the other hand, at the level of the individual gene/protein, there was not a good correlation between protein spot intensity and microarray values. We also obtained strong evidence for post-translational modification from 2-DGE and MudPIT data. We detected proteins that are annotated to function in protein synthesis, folding, modification, and degradation, as well as the presence of regulatory proteins such as transcription factors and protein kinases. Finally, sequence and evolutionary analysis of genes for active methyl group metabolisms suggests that these genes are highly conserved. Our results allow the formulation of hypotheses regarding post-translational regulation of proteins in the flower, providing new understanding about Arabidopsis flower development and physiology.
基金supported by the Key Discipline and Major Project of Dalian Science and Technology Innovation Foun-dation(No.2020JJ25CY004).
文摘Superalloys feature multi-elements and complex elemental ranges,which makes the proper composition selection difficult.In fact,more strict composition standards generally apply in practical productions.The objective of this paper is to understand and eventually to renew the composition standard via example of the most common grade Inconel 718.We have recently shown that t he alloy chemistry originates from a nearest-neighbor cluster[center-shell]plus a few next-neighbor glue atoms,or expressed in cluster for-mula[center-shell](glue atoms).By grouping the elements into Ni=(Ni,Co,Cu,Fe),Cr=(Cr,Mn,Si,Mo),and Nb=(Nb,Al,Ti),it is found that the reported alloys fall within a narrow composition zone Ni_(11.0-13.0)-Cr_(3.5-4.5)-Nb_(1) confined by cluster formulas of 16 and 18 atoms.This composition zone is also expressed in terms of 288-atom supercluster formulas,Ni_(198-208)-Cr_(63-72)-Nb_(16-18),which leads to coordi-nated elemental variations in wt.%:69.0≤Ni+Co+Cu+Fe≤72.7;19.8≤Cr+Mn+1.7 Si+0.6 Mo≤22.8;8.7≤Nb+3.2 Al+1.9 Ti≤9.8.Within this composition zone,Ni_(206)-Cr_(65.5)-Nb_(16.5) is further pinpointed and validated by our own experiments to possess the optimal match of strength and plasticity both at room and at 923 K.
基金Supported by a grant from the State Key Laboratory of Environmental Chemistry and Ecotoxicology,Chinese Academy of Sciences(No.KF2011-12)
文摘Objective： We aimed to evaluate the efficiency of serum testosterone suppression as well as the potential for agonistic stimulation of serum testosterone during chronic treatment with monthly （3.75 mg） depot formulation of domestic substitute of leuprorelin acetate microspheres for patients with metastatic prostate cancer. Methods： A total of 23 patients with metastatic prostate cancer were enrolled in the prospective study and received 6 monthly intramuscular depot injections of domestic substitute of leuprorelin acetate microspheres. Their levels and patterns of serum testosterone suppression and the potential for agonistic stimulation of serum testosterone were monitored following injection monthly （3.75 rag） depot formulation of domestic substitute of leuprorelin acetate microspheres for 24 weeks. Results： Mean testosterone was 431.4 ng/dL, 119.3 ng/dL, 28.2 ng/dL by week 1, 2, 3 and decreased to less than 15.6 ng/dL by week 4 where it remained throughout the treatment period. Median time to suppression of serum testosterone was 20.7 days. No transient minor ＂escape＂ from suppression occurred in all patients which was defined as a single testosterone value greater than 50 ng/dL once suppression was achieved. Assessment of agonistic stimulation following the second depot injection revealed no pattern of stimulation. Conclusion： We concluded that monthly （3.75 mg） depot formulation of domestic substitute of leuprorelin acetate microspheres could provide persistent, stable suppression of serum testosterone throughout the dosing intervals, and that the initial depot injection of this formulation also could provide sufficient pituitary desensitization to prevent agnostic stimulation of serum testosterone during chronic treatment.
基金This project was financially supported as National S&T Major Special Project on Major New Drug Innovation(No.2009zx09103-036)by the National Nature Science Foun-dation of China(No.30572256).
文摘A series of novel phenyl-urea derivatives which can simultaneously activate gluco kinase(GK)and peroxisome proliferator-activated receptorγ(PPARγ)was designed and prepared,and their activation of GK and PPARγ was evaluated.The structure--activity relationships of these compounds are also described.Three compounds showed potent ability to activate both GK and PPARγ.The possible binding mode of one of these compounds with GK and PPARγ were predicted by molecular docking simulation.
文摘The absence of effective therapies for castration-resistant prostate cancer(CRPC) establishes the need to develop novel therapeutic modality, such as targeted gene therapy, which is ideal for the treatment of CRPC. But its application has been limited due to lack of favorable gene vector and the reduction of "bystander effect". Consequently, scientists all over the world focus their main experimental research on the following four aspects: targeted gene, vector, transfer means and comprehensive therapy. In this paper, we reviewed the latest advances of experimental research on targeted gene therapy for prostate cancer.
基金supported by the Sichuan Science and Technology Program(2021YJ0287)National Natural Science Foundation of ChinaPR China(31772616)。
文摘Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive benefits.The objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development,to determine the optimal DF intake for gilts,and gain microbial and metabolomic insight into the underlying mechanisms involved.Methods:Seventy-six Landrace×Yorkshire(LY)crossbred replacement gilts of similar age(92.6±0.6 d;mean±standard deviation[SD])and body weight(BW,33.8±3.9 kg;mean±SD)were randomly allocated to 4 dietary treatment groups(n=19);a basal diet without extra DF intake(DF 1.0),and 3 dietary groups ingesting an extra50%(DF 1.5),75%(DF 1.75),and 100%(DF 2.0)dietary fiber mixture consisting of inulin and cellulose(1:4).Oocyte maturation and uterine development were assessed on 19 d of the 2nd oestrous cycle.Microbial diversity of faecal samples was analysed by high-throughput pyrosequencing(16S r RNA)and blood samples were subjected to untargeted metabolomics.Results:The rates of oocytes showing first polar bodies after in vitro maturation for 44 h and uterine development increased linearly with increasing DF intake;DF 1.75 gilts had a 19.8%faster oocyte maturation rate and a 48.9 cm longer uterus than DF 1.0 gilts(P<0.05).Among the top 10 microbiota components at the phylum level,8increased linearly with increasing DF level,and the relative abundance of 30 of 53 microbiota components at the genus level(>0.1%)increased linearly or quadratically with increasing DF intake.Untargeted metabolic analysis revealed significant changes in serum metabolites that were closely associated with microbiota,including serotonin,a gut-derived signal that stimulates oocyte maturation.Conclusions:The findings provide evidence of the benefits of increased DF intake by supplementing inulin and cellulose on oocyte maturation and uterine development in gilts,and new microbial and metabolomic insight into the mechanisms mediating the ef
基金Sponsored by National Natural Science Foundation of China (51090381)Foundation of Yunnan Educational Committee of China (2010Z013)
文摘Thermodynamic properties for an alloy system play an important role in the materials science and engineer- ing. Therefore, theoretical calculations having the flexibility to deal with complexity are very useful and have scien- tific meaning. The Hoch-Arpshofen model was deduced from physical principles and is applicable to binary, ternary and larger system using its binary interaction parameters only. Calculations of the activities of Fe-based liquid alloys are calculated using Hoch-Arpshofen model from data on the binary subsystems. Results for the activities for Fe-Au- Ni, Fe-Cr-Ni, Fe-Co-Cr and Fe-Co-Ni systems at required temperature are presented by Hoch-Arpshofen model. The average relative errors of prediction are 7.8%, 4.5%, 4.9~ and 2.7%, respectively. It shows that the calcu- lated results are in good agreement with the experimental data except Fe-Au-Ni system, which exhibits strong inter- action between unlike atoms. The model provides a simple, reliable and general method for calculating the activities for Fe-based liquid alloys.
文摘Most of the XY positioning stages proposed in previous studies are mainly designed by considering only a single performance indicator of the stage. As a result, the other performance indicators are relatively weak. In this study, a 2-degree-of-freedom linear compliant positioning stage （LCPS） is developed by mechatronic design to balance the interacting performance indicators and realize the desired positioning stage. The key parameters and the coupling of the structure and actuators are completely considered in the design. The LCPS consists of four voice coil motors （VCMs）, which are conformally designed for compactness, and six spatial leaf spring parallelograms. These parallelograms are serially connected for a large travel range and a high out-of-plane payload capacity. The mechatronic model is established by matrix structural analysis for structural modeling and by Kirchhof^s law for the VCMs. The sensitivities of the key parameters are analyzed, and the design parameters are subsequently determined. The analytical model of the stage is confirmed by experiments. The stage has a travel range of 4.4 mm 7.0 mm and a 0.16% area ratio of workspace to the outer dimension of the stage. The values of these performance indicators are greater than those of any existing stage reported in the literature. The closed-loop bandwidth is 9.5 Hz in both working directions. The stage can track a circular trajectory with a radius of 1.5 mm, with 40 Ixm error and a resolution of lower than 3 ~tm. The results of payload tests indicate that the stage has at least 20 kg out- of-plane payload capacity.
基金We gratefully acknowledge the financial support from the National Natural Science Foundation of China(Grant No.21576205)the Tianjin Natural Science Foundation(Grant No.18JCTPJC48600)the Training Project of Innovation Team of Colleges and Universities in Tianjin(GrantNo.TD13-5020).
文摘The increasing applications of seawater desalination technology have led to the wide usage of polyamide reverse osmosis membranes,resulting in a large number of wasted reverse osmosis membranes.In this work,the base nonwoven layer of the wasted reverse osmosis membrane was successfully modified into the hydrophobic membrane via surface deposition strategy including TiO_(2) and 1H,1H,2H,2H-perfluorooctyltrichlorosilane(PFOTS),respectively.Various techniques were applied to characterize the obtained membranes,which were then used to separate the oil–water system.The optimally modified membrane displayed good hydrophobicity with a contact angle of 135.2°±0.3°,and its oil–water separation performance was as high as 97.8%.After 20 recycle tests,the oil–water separation performance remained more than 96%,which was attributed to the film adhesion of the anchored TiO_(2) and PFOTS layer on the surface.This work might provide a new avenue for recycling the wasted reverse osmosis membrane used in oily wastewater purification.
基金financially supported by the National Natural Science Foundation of China(grants No.41572097,41472088 and 41002033)the State Scholarship Fund of China Scholarship Council
文摘Objective Researchers have recently discovered that sublacustrine sedimentary exhalative mechanism associated with volcanism,is the principle way to form lacustrine exhalative rocks.These rocks differentiate themselves from normal sedimentary rocks in their specificpetrofabric and material composition.
基金Supported by Chongqing Humanities and Social Science Key Research Project(11SKB31)Chongqing Philosophy and Social Science Planning Project(2014YBGL130)
文摘Using the survey data on 340 farmers in Chongqing City,this paper performs an empirical analysis of the factors influencing the non-point source pollution of pesticides. The results show that the older householders will apply more pesticides,which may be due to the weak physical strength and weak ability to accept the concept of advanced cultivation; the householders with high level of education will choose to use less pesticides; the pesticide application rate is negatively correlated with whether farmers have participated in agricultural technology training,that is,the farmers having participated in agricultural technology training have stronger ability to scientifically apply pesticides,and in-depth understanding of advanced agricultural production technology and positive and negative effects of pesticides,so they often choose to reduce the application rate of pesticide; the cognitive factor on the role of pesticides in better promoting the growth of crops is significant,which requires the government and relevant departments to carry out concrete publicity of effectiveness and negative impact of different pesticides during the popularization of agricultural science knowledge,to prompt farmers to have a systematic and in-depth understanding of the agricultural nonpoint source pollution caused by pesticides.
基金supported by the National Natural Science Foundation of China(51072087)Specialized Research Fund for the Doctoral Program of Higher Education(20113719110001)
文摘In this work, surface-mazelike Zn O, Cu, and Ni hierarchical structures were synthesized via a versatile ethylene glycol- mediated solvothermal method. The structure evolution of these materials bore striking similarities, including(1) initial formation of metal alkoxides precursors and(2) subsequent structural evolution of products from tiered plates to jigsaw puzzles; then to extrusion ridges, nests and spindles; and thereafter to final mazelike structures driven by persistent thermal decomposition of preformed precursors. Based on their unique surface morphologies in sinuous asymmetry, it is anticipated that such mazelike hierarchical structures may shed new light on the development of morphology-controlled adsorption and heterogeneous catalysts.
基金Supported by Major Project of Key Research Base of Humanities and Social Science of Chongqing Municipality(11SKB31)Social Science Planning Project of Chongqing Municipality(2014YBGL130)
文摘Improper use of chemical fertilizers is an essential reason for diffused pollution of agriculture. Therefore,finding out influence factors of farmers in application of chemical fertilizers will play a significant role in controlling the diffused pollution of agriculture. Through field survey,a total of 340 samples in 4 counties of Chongqing Municipality were obtained. On the basis of these samples,an empirical study was carried out. The study results indicate that farmers' application of chemical fertilizers is negatively correlated with farmers' age,education level,male labor proportion,and soil fertility,while the annual family income,agricultural production population proportion,commodity trading characteristics,and scientific fertilizer application ability fail to pass the significance test. These results will provide reference for proper application of chemical fertilizers and controlling diffused pollution.
基金This work was supported by the National Natural Science Foundation of China （Nos. J1103303, 21302185）.
文摘Two isostructural metal-organic frameworks formulated as [Gd7（L）4（μ3-OH）8（HCOO）（DMF）6（H20）].DMF·H2O （1） and [Tbv（L）4（P3-OH）8（HCOO）（DMF）5（HzO）]·DMFoH2O （2） （H3L=3,5-di（4＇-carboxylphenyl）benzoic acid） have been synthesized and structurally characterized. The building blocks of the structures are the heptanuclear lanthanide clusters of the dicubane-like type [Lnv7（μ3-OH）s]13＋ （Ln=Gd, Tb） which are formally assembled into porous three-dimensional networks through the linkage of L. Variable temperature magnetic studies down to 2 K reveal antiferromagnetic interactions in complex 1. Furthermore, the fluorescence property of 2 was also studied.
基金Financial support was provided by the National Natural Science Foundation of China (Nos. 21525209, 21621002, 21772225, and 21761142003)the Chinese Academy of Sciences (Strategic Priority Research Program (No. XDB20000000)+3 种基金Key Research Program of Frontier Sciences (No. QYZDB-SSW-SLH040))Shanghai Science and Technology Commission (Nos. 15JC1400400 and 17XD1404600)the National Program for Support of TopNotch Young Professionals of Chinathe K. C. Wong Education Foundation
文摘On the basis of the mild transformation of alkenyl triflates into alkynes promoted by LiCl, a one-pot protocol using alkenyl triflate precursors was developed for copper-mediated azide–alkyne cycloaddition. This protocol may provide an opportunity of sequentially click reactions for the construction of bifunctional probes in chemical biology studies.
基金Supported by Special Forestry Science and Technology Innovation Project in Guangdong Province(2012kjcx017-02)
文摘In 2007,one living Ermia mangshanensis was found in the Shapingpian area at the junction of South China Tiger Nature Reserve in North Guangdong and Hunan Mangshan National Nature Reserve.To learn more about Ermia mangshanensis resource,the researchers in South China Tiger Nature Reserve in North Guangdong and Hunan Mangshan National Nature Reserve conducted field survey of Ermia mangshanensis in the Shapingpian area during the period 2010- 2012.The results show that there is Ermia mangshanensis in the Shapingpian area of South China Tiger Nature Reserve at the elevation of 800- 1200 m,and the population number is estimated at 48- 80.
