BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. We analyzed the expression of miclear-transcription factor-kappa B (NF-kappa B) during hepatocarcinogenesis in order to evaluate i...BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. We analyzed the expression of miclear-transcription factor-kappa B (NF-kappa B) during hepatocarcinogenesis in order to evaluate its dynamic expression and its clinical value in the development and diagnosis of HCC. METHODS: Hepatoma models were induced by oral administration of 2-acetamidoflurene (2-FAA) to male Sprague-Dawley rats. Morphological changes were observed after hematoxylin and eosin staining. The cellular distribution of NF-kappa B expression during different stages of cancer development was investigated by immunohistochemistry, and the level of NF-kappa B expression in liver tissues was quantitatively analyzed by ELISA. The gene fragments of hepatic NF-kappa B were amplified by nested-polymerase chain reaction assay. RESULTS: Hepatocytes showed vacuole-like degeneration during the early stages, then had a hyperplastic nodal appearance during the middle stages, and finally progressed to tubercles of cancerous nests with high differentiation. The NF-kappa B-positive material was buff-colored, fine particles localized in the nucleus, and the incidence of NF-kappa B-positive cells was 81.8% in degeneration, 83.3% in precancerous lesions, and 100% in cancerous tissues. All of these values were higher than those in controls (P<0.01). Hepatic NF-kappa B expression and hepatic NF-kappa B-mRNA were also higher during the course of HCC development (P<0.01). CONCLUSION: The NF-kappa B signal transduction pathway is activated during the early stages of HCC development, and its abnormal expression may be associated with the occurrence of HCC.展开更多
目的比较后路小切口与后侧标准入路切口髋关节置换术的临床疗效。方法将120例老年股骨颈骨折患者按手术切口不同分为A组(采用后路小切口髋关节置换术治疗,60例)和B组(采用后侧标准入路切口髋关节置换术治疗,60例)。比较两组切口长度、...目的比较后路小切口与后侧标准入路切口髋关节置换术的临床疗效。方法将120例老年股骨颈骨折患者按手术切口不同分为A组(采用后路小切口髋关节置换术治疗,60例)和B组(采用后侧标准入路切口髋关节置换术治疗,60例)。比较两组切口长度、术中出血量、术后引流量、手术时间、术后下地时间、术后1 d VAS评分及术后6个月Harris评分。结果患者均获得随访,时间6~13(8.23±3.91)个月。切口长度、术中出血量、术后引流量、术后下地时间、术后1 d疼痛VAS评分A组均优于B组(P<0.01)。手术时间、术后6个月Harris评分两组比较差异均无统计学意义(P>0.05)。结论与后侧标准入路切口比较,后路小切口髋关节置换术治疗老年股骨颈骨折具有切口小、术中出血少、术后引流量少、患者术后早期恢复快等优点。展开更多
目的研究分析感染可能性评分(infection probability score,IPS)对于行输尿管软镜术后患者发生尿源性脓毒血症的筛查预测效果。方法研究纳入医院2016年1月-2017年12月收治的行输尿管软镜术治疗的196例患者,以炎症反应作为诊断标准,遵照...目的研究分析感染可能性评分(infection probability score,IPS)对于行输尿管软镜术后患者发生尿源性脓毒血症的筛查预测效果。方法研究纳入医院2016年1月-2017年12月收治的行输尿管软镜术治疗的196例患者,以炎症反应作为诊断标准,遵照随机分配原则分为对照组108例与试验组88例,对试验组患者进行细菌培养分析,并检测对比两组患者血清降钙素原(procalcitonin,PCT)、C-反应蛋白(C-reactionprotein,CRP)以及白细胞(white blood cell,WBC)水平,对IPS评分在术后尿源性脓毒血症的预筛查中的价值进行分析。结果试验组中88例患者共培养病原菌102株,其中革兰阴性菌82株占80.39%、革兰阳性菌20株占19.61%,革兰阴性菌中以大肠埃希菌为主;血清指标检测结果显示,试验组患者PCT、CRP以及WBC水平均高于对照组患者(P<0.05);试验组患者IPS评分均高于对照组患者(P<0.05)。结论输尿管软镜术后尿源性脓毒血症患者主要感染病原菌为革兰阴性菌,患者CRP及WBC水平均有所升高,应用IPS评分可有效鉴别患者术后感染情况,对于尿源性脓毒血症的诊断准确性较高,在临床预测筛查中有一定的应用价值。展开更多
基金supported by grants from the Project of Elitist Peak in Six Fields(No.2006-B-063)the Projectof Medical Sciences(H200727),the Bureau of Health,Jiangsu Province,China
文摘BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. We analyzed the expression of miclear-transcription factor-kappa B (NF-kappa B) during hepatocarcinogenesis in order to evaluate its dynamic expression and its clinical value in the development and diagnosis of HCC. METHODS: Hepatoma models were induced by oral administration of 2-acetamidoflurene (2-FAA) to male Sprague-Dawley rats. Morphological changes were observed after hematoxylin and eosin staining. The cellular distribution of NF-kappa B expression during different stages of cancer development was investigated by immunohistochemistry, and the level of NF-kappa B expression in liver tissues was quantitatively analyzed by ELISA. The gene fragments of hepatic NF-kappa B were amplified by nested-polymerase chain reaction assay. RESULTS: Hepatocytes showed vacuole-like degeneration during the early stages, then had a hyperplastic nodal appearance during the middle stages, and finally progressed to tubercles of cancerous nests with high differentiation. The NF-kappa B-positive material was buff-colored, fine particles localized in the nucleus, and the incidence of NF-kappa B-positive cells was 81.8% in degeneration, 83.3% in precancerous lesions, and 100% in cancerous tissues. All of these values were higher than those in controls (P<0.01). Hepatic NF-kappa B expression and hepatic NF-kappa B-mRNA were also higher during the course of HCC development (P<0.01). CONCLUSION: The NF-kappa B signal transduction pathway is activated during the early stages of HCC development, and its abnormal expression may be associated with the occurrence of HCC.
文摘目的比较后路小切口与后侧标准入路切口髋关节置换术的临床疗效。方法将120例老年股骨颈骨折患者按手术切口不同分为A组(采用后路小切口髋关节置换术治疗,60例)和B组(采用后侧标准入路切口髋关节置换术治疗,60例)。比较两组切口长度、术中出血量、术后引流量、手术时间、术后下地时间、术后1 d VAS评分及术后6个月Harris评分。结果患者均获得随访,时间6~13(8.23±3.91)个月。切口长度、术中出血量、术后引流量、术后下地时间、术后1 d疼痛VAS评分A组均优于B组(P<0.01)。手术时间、术后6个月Harris评分两组比较差异均无统计学意义(P>0.05)。结论与后侧标准入路切口比较,后路小切口髋关节置换术治疗老年股骨颈骨折具有切口小、术中出血少、术后引流量少、患者术后早期恢复快等优点。
文摘目的研究分析感染可能性评分(infection probability score,IPS)对于行输尿管软镜术后患者发生尿源性脓毒血症的筛查预测效果。方法研究纳入医院2016年1月-2017年12月收治的行输尿管软镜术治疗的196例患者,以炎症反应作为诊断标准,遵照随机分配原则分为对照组108例与试验组88例,对试验组患者进行细菌培养分析,并检测对比两组患者血清降钙素原(procalcitonin,PCT)、C-反应蛋白(C-reactionprotein,CRP)以及白细胞(white blood cell,WBC)水平,对IPS评分在术后尿源性脓毒血症的预筛查中的价值进行分析。结果试验组中88例患者共培养病原菌102株,其中革兰阴性菌82株占80.39%、革兰阳性菌20株占19.61%,革兰阴性菌中以大肠埃希菌为主;血清指标检测结果显示,试验组患者PCT、CRP以及WBC水平均高于对照组患者(P<0.05);试验组患者IPS评分均高于对照组患者(P<0.05)。结论输尿管软镜术后尿源性脓毒血症患者主要感染病原菌为革兰阴性菌,患者CRP及WBC水平均有所升高,应用IPS评分可有效鉴别患者术后感染情况,对于尿源性脓毒血症的诊断准确性较高,在临床预测筛查中有一定的应用价值。
