Allicin, an antioxidant, is known for providing garlic with its unique fragrance and taste, as well as for its antimicrobial properties. Black garlic, a fermented form of garlic, contains higher levels of antioxidants...Allicin, an antioxidant, is known for providing garlic with its unique fragrance and taste, as well as for its antimicrobial properties. Black garlic, a fermented form of garlic, contains higher levels of antioxidants than fresh garlic. Antioxidants play a vital role in alleviating cellular stress during viral infections. Viral infections result in oxidative stress through the production of reactive oxidative species (ROS). A prolonged state of oxidative stress can result in cell death, DNA damage, and disease progression. In this study, black garlic extract (BGE) is evaluated for its ability to mitigate cytopathic effects and oxidative stress caused by herpes simplex virus-2 (HSV-2) infections in vitro. Antiviral assays were performed to determine the percent of viral inhibition resulting from treatment with the BGE. ROS-Glo<sup>TM</sup> H<sub>2</sub>O<sub>2</sub> assays were then completed to measure the post-infection ROS levels of BGE-treated virus and cells. The results thus far suggest that BGE may inhibit viral infection and decrease levels of oxidative stress.展开更多
The purpose of this study was to investigate the effect of curcumin-treated Herpes simplex virus-1 (HSV-1) and Herpes simplex virus-2 (HSV-2) virions in cultured Vero cells. Previous studies have indicated that curcum...The purpose of this study was to investigate the effect of curcumin-treated Herpes simplex virus-1 (HSV-1) and Herpes simplex virus-2 (HSV-2) virions in cultured Vero cells. Previous studies have indicated that curcumin, a polyphenol extracted from the plant Curcuma longa, has demonstrated antiviral properties against a variety of viruses. After establishing the maximum non-cytotoxic concentrations of curcumin on Vero cells, HSV-1 and HSV-2 virions were treated with varying concentrations of curcumin. The effect on infectivity was determined by antiviral assays, using WST-1, plaque assays, adsorption and penetration assays. Treating HSV-1 and HSV-2 viruses with curcumin, at a concentration of 30 μM, reduces the production of infectious HSV-1 and HSV-2 virions in cultured Vero cells by interfering with the adsorption process. These results support the potential of curcumin to be used as a therapeutic agent to reduce the transmission of HSV-1 and HSV-2.展开更多
<div style="text-align:justify;"> <span style="font-family:Verdana;">Enteroviruses are responsible for emerging diseases which cause diverse symptoms and may result in neurological comp...<div style="text-align:justify;"> <span style="font-family:Verdana;">Enteroviruses are responsible for emerging diseases which cause diverse symptoms and may result in neurological complications. An antiviral with multiple mechanisms of action can help prevent enterovirus mediated disease despite differences in the pathogenesis between enteroviruses, including the recently identified enterovirus 69 (EV-69) for which pathogenesis is not well understood. This study investigated the efficacy of epigallocatechin-3-gallate stearate (EGCG-S), a modified form of the antioxidant green tea catechin epigallocatechin-3-gallate (EGCG), in inhibiting EV-69 infection of lung fibroblast cells </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;">. Treatment with EGCG-S resulted in moderate protection from EV-69 mediated cytotoxicity as demonstrated by increased metabolic activity as well as maintenance of cell morphology and mitochondrial function. These effects were correlated with reduced hydrogen peroxide production in infected cells following EGCG-S treatment with concentrations less than 100 μM, suggesting a role for inhibition of EV-69 mediated oxidative stress. This study provides insight into characteristics of EV-69 infection as well as the efficacy of EGCG-S mediated inhibition of EV-69 infection.</span> </div>展开更多
<span style="font-family:'';font-size:10pt;"><span style="font-size:12px;font-family:Verdana;">Pathogenic spore-forming bacteria pose high risks to healthcare settings, as well ...<span style="font-family:'';font-size:10pt;"><span style="font-size:12px;font-family:Verdana;">Pathogenic spore-forming bacteria pose high risks to healthcare settings, as well as in the food and beverage industries. We reported recently that novel alcohol-based formulations containing plant-derived compounds, including epigallocatechin-3-gallate-palmitate (EGCG-P), a green tea polyphenol ester, provide > 99.99% inactivation of bacterial spores within 60 sec. Based on recently published data from our group and others, we hypothesize that a combination of EGCG-P and alcohol formulated with other plant-derived ingredients would achieve high sporicidal efficacy against a wide spectrum of bacterial spores and can provide novel hand hygiene methods against bacterial spores without toxicity. The objectives of the current study were to optimize </span><span style="font-size:12px;font-family:Verdana;">two novel formulations with combinations of glycerol, citric acid, and EGCG-P</span><span style="font-size:12px;font-family:Verdana;"> to increase sporicidal activity and explore the rapid inactivation mechanisms and suitability for sporicidal products with broad-spectrum activities against aerobic and anaerobic bacterial spores. Methods included suspension testing of two formulations against spores from </span><i><span style="font-size:12px;font-family:Verdana;">Bacillus cereus</span></i><span style="font-size:12px;font-family:Verdana;"> and </span><i><span style="font-size:12px;font-family:Verdana;">Clostridium sporogenes</span></i><span style="font-size:12px;font-family:Verdana;">, quantification of spore germination, and scanning electron microscopy. The results demonstrated that these novel formulations were able to reduce spore germination by >99.999% after 30 sec exposure in </span><span style="font-size:12px;font-family:Verdana;">suspension tests, and rapidly caused physical damage to the spores. Additional</span></span><span style="font-family:'';font-size:10pt;"> </span><span style="line-height:1.5;font-family:Ver展开更多
【据Journal of Gastroenterology and Hepatology 2020年9月报道】题:质子泵抑制剂的使用和脂肪性肝病的风险:一项全国范围的队列研究(作者Pyo JH等)质子泵抑制剂(PPI)诱导的疑病症可以改变肠道菌群的组成,诱导小肠细菌过度生长,这已...【据Journal of Gastroenterology and Hepatology 2020年9月报道】题:质子泵抑制剂的使用和脂肪性肝病的风险:一项全国范围的队列研究(作者Pyo JH等)质子泵抑制剂(PPI)诱导的疑病症可以改变肠道菌群的组成,诱导小肠细菌过度生长,这已被认为是通过肠-肝轴促进脂肪肝的发展。在这项研究中,旨在调查PPI的使用与脂肪性肝病风险之间的关系。展开更多
文摘Allicin, an antioxidant, is known for providing garlic with its unique fragrance and taste, as well as for its antimicrobial properties. Black garlic, a fermented form of garlic, contains higher levels of antioxidants than fresh garlic. Antioxidants play a vital role in alleviating cellular stress during viral infections. Viral infections result in oxidative stress through the production of reactive oxidative species (ROS). A prolonged state of oxidative stress can result in cell death, DNA damage, and disease progression. In this study, black garlic extract (BGE) is evaluated for its ability to mitigate cytopathic effects and oxidative stress caused by herpes simplex virus-2 (HSV-2) infections in vitro. Antiviral assays were performed to determine the percent of viral inhibition resulting from treatment with the BGE. ROS-Glo<sup>TM</sup> H<sub>2</sub>O<sub>2</sub> assays were then completed to measure the post-infection ROS levels of BGE-treated virus and cells. The results thus far suggest that BGE may inhibit viral infection and decrease levels of oxidative stress.
文摘The purpose of this study was to investigate the effect of curcumin-treated Herpes simplex virus-1 (HSV-1) and Herpes simplex virus-2 (HSV-2) virions in cultured Vero cells. Previous studies have indicated that curcumin, a polyphenol extracted from the plant Curcuma longa, has demonstrated antiviral properties against a variety of viruses. After establishing the maximum non-cytotoxic concentrations of curcumin on Vero cells, HSV-1 and HSV-2 virions were treated with varying concentrations of curcumin. The effect on infectivity was determined by antiviral assays, using WST-1, plaque assays, adsorption and penetration assays. Treating HSV-1 and HSV-2 viruses with curcumin, at a concentration of 30 μM, reduces the production of infectious HSV-1 and HSV-2 virions in cultured Vero cells by interfering with the adsorption process. These results support the potential of curcumin to be used as a therapeutic agent to reduce the transmission of HSV-1 and HSV-2.
文摘<div style="text-align:justify;"> <span style="font-family:Verdana;">Enteroviruses are responsible for emerging diseases which cause diverse symptoms and may result in neurological complications. An antiviral with multiple mechanisms of action can help prevent enterovirus mediated disease despite differences in the pathogenesis between enteroviruses, including the recently identified enterovirus 69 (EV-69) for which pathogenesis is not well understood. This study investigated the efficacy of epigallocatechin-3-gallate stearate (EGCG-S), a modified form of the antioxidant green tea catechin epigallocatechin-3-gallate (EGCG), in inhibiting EV-69 infection of lung fibroblast cells </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;">. Treatment with EGCG-S resulted in moderate protection from EV-69 mediated cytotoxicity as demonstrated by increased metabolic activity as well as maintenance of cell morphology and mitochondrial function. These effects were correlated with reduced hydrogen peroxide production in infected cells following EGCG-S treatment with concentrations less than 100 μM, suggesting a role for inhibition of EV-69 mediated oxidative stress. This study provides insight into characteristics of EV-69 infection as well as the efficacy of EGCG-S mediated inhibition of EV-69 infection.</span> </div>
文摘<span style="font-family:'';font-size:10pt;"><span style="font-size:12px;font-family:Verdana;">Pathogenic spore-forming bacteria pose high risks to healthcare settings, as well as in the food and beverage industries. We reported recently that novel alcohol-based formulations containing plant-derived compounds, including epigallocatechin-3-gallate-palmitate (EGCG-P), a green tea polyphenol ester, provide > 99.99% inactivation of bacterial spores within 60 sec. Based on recently published data from our group and others, we hypothesize that a combination of EGCG-P and alcohol formulated with other plant-derived ingredients would achieve high sporicidal efficacy against a wide spectrum of bacterial spores and can provide novel hand hygiene methods against bacterial spores without toxicity. The objectives of the current study were to optimize </span><span style="font-size:12px;font-family:Verdana;">two novel formulations with combinations of glycerol, citric acid, and EGCG-P</span><span style="font-size:12px;font-family:Verdana;"> to increase sporicidal activity and explore the rapid inactivation mechanisms and suitability for sporicidal products with broad-spectrum activities against aerobic and anaerobic bacterial spores. Methods included suspension testing of two formulations against spores from </span><i><span style="font-size:12px;font-family:Verdana;">Bacillus cereus</span></i><span style="font-size:12px;font-family:Verdana;"> and </span><i><span style="font-size:12px;font-family:Verdana;">Clostridium sporogenes</span></i><span style="font-size:12px;font-family:Verdana;">, quantification of spore germination, and scanning electron microscopy. The results demonstrated that these novel formulations were able to reduce spore germination by >99.999% after 30 sec exposure in </span><span style="font-size:12px;font-family:Verdana;">suspension tests, and rapidly caused physical damage to the spores. Additional</span></span><span style="font-family:'';font-size:10pt;"> </span><span style="line-height:1.5;font-family:Ver
文摘【据Journal of Gastroenterology and Hepatology 2020年9月报道】题:质子泵抑制剂的使用和脂肪性肝病的风险:一项全国范围的队列研究(作者Pyo JH等)质子泵抑制剂(PPI)诱导的疑病症可以改变肠道菌群的组成,诱导小肠细菌过度生长,这已被认为是通过肠-肝轴促进脂肪肝的发展。在这项研究中,旨在调查PPI的使用与脂肪性肝病风险之间的关系。
