TRIM71 is an RNA-binding protein with ubiquitin ligase activity.Numerous functions of mammalian TRIM71,including cell cycle regulation,embryonic stem cell(ESC)self-renewal,and reprogramming of pluripotent stem cells,a...TRIM71 is an RNA-binding protein with ubiquitin ligase activity.Numerous functions of mammalian TRIM71,including cell cycle regulation,embryonic stem cell(ESC)self-renewal,and reprogramming of pluripotent stem cells,are related to its RNA-binding property.We previously reported that a long noncoding RNA(lnc RNA)Trincr1 interacts with mouse TRIM71(m TRIM71)to repress FGF/ERK pathway in mouse ESCs(m ESCs).Herein,we identify an RNA motif specifically recognized by m TRIM71 from Trincr1 RNA,and solve the crystal structure of the NHL domain of m TRIM71 complexed with the RNA motif.Similar to the zebrafish TRIM71,m TRIM71 binds to a stem-loop structured RNA fragment of Trincr1,and an adenosine base at the loop region is crucial for the m TRIM71 interaction.We map similar hairpin RNAs preferably bound by TRIM71 in the m RNA UTRs of the cell-cycle related genes regulated by TRIM71.Furthermore,we identify key residues of m TRIM71,conserved among mammalian TRIM71 proteins,required for the RNA-binding property.Single-site mutations of these residues significantly impair the binding of TRIM71 to hairpin RNAs in vitro and to m RNAs of Cdkn1a/p21 and Rbl2/p130 in m ESCs.Furthermore,congenital hydrocephalus(CH)specific mutation of m TRIM71 impair its binding to the RNA targets as well.These results reveal molecular mechanism behind the recognition of RNA by mammalian TRIM71 and provide insights into TRIM71 related diseases.展开更多
The ribosome is a cellular system responsible for translating the nucleotide code of mRNA into proteins.In human cells,this complex consists of four distinct ribosomal RNAs(rRNAs):The 28S,18S,5.8S,and 5S rRNAs,along w...The ribosome is a cellular system responsible for translating the nucleotide code of mRNA into proteins.In human cells,this complex consists of four distinct ribosomal RNAs(rRNAs):The 28S,18S,5.8S,and 5S rRNAs,along with 80 ribosomal proteins.Polymerase Ⅰ is responsible for synthesizing the 28S,18S,and 5.8S rRNAs,while polymerase Ⅲ is responsible for synthesizing the 5S rRNA[1].展开更多
水系金属空气电池具有理论能量密度高、安全性高等优点,但受限于金属阳极(如锌、铁、铝、镁)的电化学不可逆性以及碱性电解质对大气中二氧化碳的化学不稳定性.本工作首次设计了一种可充电的铋-空气电池,该电池使用了非碱性的三氟甲磺酸...水系金属空气电池具有理论能量密度高、安全性高等优点,但受限于金属阳极(如锌、铁、铝、镁)的电化学不可逆性以及碱性电解质对大气中二氧化碳的化学不稳定性.本工作首次设计了一种可充电的铋-空气电池,该电池使用了非碱性的三氟甲磺酸铋(Bi(OTf)_(3))水系电解质.得益于三电子反应和相对于标准氢电极+0.32 V的高电位,铋金属负极具有383 mA h g^(−1)的高比容量和1000次循环的良好稳定性,以及99.6%高库仑效率.铋金属负极在Bi(OTf)_(3)电解液中无腐蚀、钝化和析氢等副反应.此外,非碱性的铋-空气电池通过三氧化二铋(Bi_(2)O_(3))的可逆形成/分解,在环境空气中实现了长期运行稳定性(>200 h).这项工作为探索新型水系金属空气电池作为安全稳定的电源系统提供了新思路.展开更多
Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magne...Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magnetic storage.However, reversed magnetic domains come into being with the increasing layer repetition ‘N’ to reduce magneto-static energy, resulting in the remarkable diminishment of the remanent magnetization(Mr). As a result, the product of Mrand thickness(i.e., the remanent moment-thickness product, Mrt), a key parameter in magnetic recording for reliable data storing and reading, also decreases dramatically. To overcome this issue, we deposit an ultra-thick granular [Co/Pt]80multilayer with a total thickness of 68 nm on granular SiNxbuffer layer. The Mrt value, Mrto saturation magnetization(Ms) ratio as well as out of plane(OOP) coercivity(Hcoop) are high up to 2.97 memu/cm^(2), 67%, and 1940 Oe(1 Oe = 79.5775 A·m^(-1)),respectively, which is remarkably improved compared with that of continuous [Co/Pt]80multilayers. That is because large amounts of grain boundaries in the granular multilayers can efficiently impede the propagation and expansion of reversed magnetic domains, which is verified by experimental investigations and micromagnetic simulation results. The simulation results also indicate that the value of Mrt, Mr/Msratio, and Hcoopcan be further improved through optimizing the granule size, which can be experimentally realized by manipulating the process parameter of SiNxbuffer layer. This work provides an alternative solution for achieving high Mrt value in ultra-thick Co/Pt multilayers, which is of unneglectable potential in applications of high-density magnetic recording.展开更多
基金the Chinese Ministry of Science and Technology,the National Natural Science Foundation of China(2019YFA0508902,32170549,32371315,2021YFA1100200,and 91940302)Haihe Laboratory of Cell Ecosystem Innovation Fund(22HHXBSS00021)。
文摘TRIM71 is an RNA-binding protein with ubiquitin ligase activity.Numerous functions of mammalian TRIM71,including cell cycle regulation,embryonic stem cell(ESC)self-renewal,and reprogramming of pluripotent stem cells,are related to its RNA-binding property.We previously reported that a long noncoding RNA(lnc RNA)Trincr1 interacts with mouse TRIM71(m TRIM71)to repress FGF/ERK pathway in mouse ESCs(m ESCs).Herein,we identify an RNA motif specifically recognized by m TRIM71 from Trincr1 RNA,and solve the crystal structure of the NHL domain of m TRIM71 complexed with the RNA motif.Similar to the zebrafish TRIM71,m TRIM71 binds to a stem-loop structured RNA fragment of Trincr1,and an adenosine base at the loop region is crucial for the m TRIM71 interaction.We map similar hairpin RNAs preferably bound by TRIM71 in the m RNA UTRs of the cell-cycle related genes regulated by TRIM71.Furthermore,we identify key residues of m TRIM71,conserved among mammalian TRIM71 proteins,required for the RNA-binding property.Single-site mutations of these residues significantly impair the binding of TRIM71 to hairpin RNAs in vitro and to m RNAs of Cdkn1a/p21 and Rbl2/p130 in m ESCs.Furthermore,congenital hydrocephalus(CH)specific mutation of m TRIM71 impair its binding to the RNA targets as well.These results reveal molecular mechanism behind the recognition of RNA by mammalian TRIM71 and provide insights into TRIM71 related diseases.
基金supported by the National Key Research and Development Program of China(2022YFC2601800)the Key-Area Research and Development Program of Guangdong Province(2023B1111020007)+6 种基金the National Natural Science Foundation of China(32161143017,82173833,81973343,and 82272658)the Guangdong Basic and Applied Basic Research Foundation(2023B1515040006)the Open Program of Shenzhen Bay Laboratory(SZBL202009051006)the Guangdong Provincial Key Laboratory of Construction Foundation(2023B1212060022)the Sichuan Science and Technology Program(2022JDRC0042 and 2022NSFSC0776)the Joint Research Foundation of Chengdu Medical College and the Seventh People’s Hospital(2022LHTD-02,2021LHJYPJ-08,and 2022488)the Shenzhen Bay Scholars Program。
文摘The ribosome is a cellular system responsible for translating the nucleotide code of mRNA into proteins.In human cells,this complex consists of four distinct ribosomal RNAs(rRNAs):The 28S,18S,5.8S,and 5S rRNAs,along with 80 ribosomal proteins.Polymerase Ⅰ is responsible for synthesizing the 28S,18S,and 5.8S rRNAs,while polymerase Ⅲ is responsible for synthesizing the 5S rRNA[1].
基金financially supported by the Science and Technology Commission of Shanghai Municipality(STCSM,21511104900 and 20JC1414902)the National Natural Science Foundation of China(52222310).
文摘水系金属空气电池具有理论能量密度高、安全性高等优点,但受限于金属阳极(如锌、铁、铝、镁)的电化学不可逆性以及碱性电解质对大气中二氧化碳的化学不稳定性.本工作首次设计了一种可充电的铋-空气电池,该电池使用了非碱性的三氟甲磺酸铋(Bi(OTf)_(3))水系电解质.得益于三电子反应和相对于标准氢电极+0.32 V的高电位,铋金属负极具有383 mA h g^(−1)的高比容量和1000次循环的良好稳定性,以及99.6%高库仑效率.铋金属负极在Bi(OTf)_(3)电解液中无腐蚀、钝化和析氢等副反应.此外,非碱性的铋-空气电池通过三氧化二铋(Bi_(2)O_(3))的可逆形成/分解,在环境空气中实现了长期运行稳定性(>200 h).这项工作为探索新型水系金属空气电池作为安全稳定的电源系统提供了新思路.
基金supported by the National Natural Science Foundation of China (Grant No. 51901008)the National Key Research and Development Program of China (Grant No. 2021YFB3201800)。
文摘Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magnetic storage.However, reversed magnetic domains come into being with the increasing layer repetition ‘N’ to reduce magneto-static energy, resulting in the remarkable diminishment of the remanent magnetization(Mr). As a result, the product of Mrand thickness(i.e., the remanent moment-thickness product, Mrt), a key parameter in magnetic recording for reliable data storing and reading, also decreases dramatically. To overcome this issue, we deposit an ultra-thick granular [Co/Pt]80multilayer with a total thickness of 68 nm on granular SiNxbuffer layer. The Mrt value, Mrto saturation magnetization(Ms) ratio as well as out of plane(OOP) coercivity(Hcoop) are high up to 2.97 memu/cm^(2), 67%, and 1940 Oe(1 Oe = 79.5775 A·m^(-1)),respectively, which is remarkably improved compared with that of continuous [Co/Pt]80multilayers. That is because large amounts of grain boundaries in the granular multilayers can efficiently impede the propagation and expansion of reversed magnetic domains, which is verified by experimental investigations and micromagnetic simulation results. The simulation results also indicate that the value of Mrt, Mr/Msratio, and Hcoopcan be further improved through optimizing the granule size, which can be experimentally realized by manipulating the process parameter of SiNxbuffer layer. This work provides an alternative solution for achieving high Mrt value in ultra-thick Co/Pt multilayers, which is of unneglectable potential in applications of high-density magnetic recording.
基金supported by the National Natural Science Foundation of China(21872104,51908408,21872163,and 22072090)the National Key Research and Development Program of China(2017YFB0602200,2020YFA0211000,and 2020YFA0211003)+3 种基金the Innovative Research Team of Tianjin Municipal Education Commission(TD13-5008)Tianjin Science and Technology Planning Project(21ZYQCSY00050)the support from the Natural Science Foundation of Tianjin for Distinguished Young Scholar(20JCJQJC00150)the support from the Tencent Foundation through the XPLORER PRIZE。
