Displacement damage effects on the charge-coupled device(CCD)induced by neutrons at the back-streaming white neutron source(Back-n)in the China Spallation Neutron Source(CSNS)are analyzed according to an online irradi...Displacement damage effects on the charge-coupled device(CCD)induced by neutrons at the back-streaming white neutron source(Back-n)in the China Spallation Neutron Source(CSNS)are analyzed according to an online irradiation experiment.The hot pixels,random telegraph signal(RTS),mean dark signal,dark current and dark signal non-uniformity(DSNU)induced by Back-n are presented.The dark current is calculated according to the mean dark signal at various integration times.The single-particle displacement damage and transient response are also observed based on the online measurement data.The trends of hot pixels,mean dark signal,DSNU and RTS degradation are related to the integration time and irradiation fluence.The mean dark signal,dark current and DSNU2 are nearly linear with neutron irradiation fluence when nearly all the pixels do not reach saturation.In addition,the mechanisms of the displacement damage effects on the CCD are demonstrated by combining the experimental results and technology computer-aided design(TCAD)simulation.Radiation-induced traps in the space charge region of the CCD will act as generation/recombination centers of electron-hole pairs,leading to an increase in the dark signal.展开更多
BACKGROUND We present a case of focal lymphoblastic transformation to erythroid leukemia following acute myeloblastic transformation in a patient with chronic myelogenous leukemia(CML)and discuss its mechanism of occu...BACKGROUND We present a case of focal lymphoblastic transformation to erythroid leukemia following acute myeloblastic transformation in a patient with chronic myelogenous leukemia(CML)and discuss its mechanism of occurrence and development.CASE SUMMARY The presence of the Philadelphia(Ph)chromosome was identified through karyotype analysis,while the BCR-ABL fusion gene was detected using quantitative real-time polymerase chain reaction of the peripheral blood sample.Fluorescence in situ hybridization was used to detect the expression of the BCRABL gene in the lymphoma.Antigen expression and gene mutations in the primitive cells were detected by flow cytometry.The analysis confirmed the presence of CML along with focal lymphoblastic transformation to erythroid leukemia.Additionally,the patient was found to have secondary erythroid leukemia,along with multiple new gene mutations and abnormalities in complex karyotypes of chromosomes.CONCLUSION Our findings suggest a possible molecular basis for the focal lymphoblastic transformation secondary to myeloblastic transformation in patients with CML.展开更多
On the basis of interpretation of comprehensive geophysical data and foreign data analysis,there existed a lot of overburden detachment shear thrust faults along the southeastern margin of Nansha Trough,which composed...On the basis of interpretation of comprehensive geophysical data and foreign data analysis,there existed a lot of overburden detachment shear thrust faults along the southeastern margin of Nansha Trough,which composed imbricated overthrust nappe structure.Thrust-faulted nappe structure pattern is determined in this area,which consists of frontal fault zone,thrust fault-folded zone and root zone structures,and presents regularly zonation on plane.The detail description of the structural geometrical characteristics is given in shallow thrust fault zone,and the kinematical mechanism of thrust fault nappe structure is furtherly discussed.Overthrnst nappe structure in this area is resulted from island arc-continent collision and orogenic activities.展开更多
Background:Infliximab(IFX)is the first-line treatment for patients with Crohn’s disease(CD)and is noted for its relatively high cost.The therapeutic efficacy of IFX has noticeable individual differences.Known single-...Background:Infliximab(IFX)is the first-line treatment for patients with Crohn’s disease(CD)and is noted for its relatively high cost.The therapeutic efficacy of IFX has noticeable individual differences.Known single-gene polymorphisms(SNPs)are inadequate for predicting non-response to IFX.In this study,we aimed to identify new genetic factors associated with IFX-therapy failure and to predict non-response to IFX by developing a multivariate predictive model.Methods:In this retrospective study,we collected and analysed the data of Chinese patients with CD who received IFX therapy at one hospital between June 2013 and June 2019.Primary non-response(PNR)and non-durable response(NDR)were evaluated using a simple endoscopic score for CD(SES-CD).A total of 125 SNPs within 44 genes were genotyped.A multivariate logistic-regression model was established to predict non-response to IFX.An area-under-the-receiver-operatingcharacteristics curve(AUROC)was applied to evaluate the predictive model performance.Results:Forty-two of 206(20.4%)patients experienced PNR and 15 of 159(9.4%)patients experienced NDR.Nine SNPs were associated with PNR(P<0.05).A PNR predictive model was established,incorporating 2-week high-sensitivity C-reactive protein(hs-CRP),rs61886887,rs61740234,rs357291,rs2269330,and rs111504845,and the AUROC on training and testing data sets were 0.818(P<0.001)and 0.888(P<0.001),respectively.At week 14,hs-CRP levels≥2.25 mg/L were significantly associated with NDR(AUROC=0.815,P<0.001).PNR-associated SNPs were not mutually associated with NDR,suggesting distinct mechanisms between PNR and NDR.Conclusion:Genetic polymorphisms are significantly associated with response to IFX among Chinese CD patients.展开更多
A thin oriented bacteriorhodopsin(bR)film is deposited on a stainless steel slide by use of the electrophoretic sedimentation method.A junction is made with electrolyte gels having a counterelectrode to construct a bR...A thin oriented bacteriorhodopsin(bR)film is deposited on a stainless steel slide by use of the electrophoretic sedimentation method.A junction is made with electrolyte gels having a counterelectrode to construct a bR-based photoelectric detector.The photoelectric response signal to a 10ns laser pulse is measured.A theory on the photoelectric kinetics of bR is developed based on the concept of the charge displacement current and the bR photocycle rate equations.Comparison between the theoretical and experimental results proves that the bR photoelectric response to a short laser pulse is a multi-exponential process.The decay time constants and amplitudes of each exponential component are obtained by data fitting.展开更多
Background:Trough levels of the post-induction serum infliximab(IFX)are associated with short-term and long-term responses of Crohn’s disease patients to IFX,but the inter-individual differences are large.We aimed to...Background:Trough levels of the post-induction serum infliximab(IFX)are associated with short-term and long-term responses of Crohn’s disease patients to IFX,but the inter-individual differences are large.We aimed to elucidate whether single gene polymorphisms(SNPs)within FCGR3A,ATG16L1,C1orf106,OSM,OSMR,NF-jB1,IL1RN,and IL10 partially account for these differences and employed a multivariate regression model to predict patients’post-induction IFX levels.Methods:The retrospective study included 189 Crohn’s disease patients undergoing IFX therapy.Post-induction IFX levels were measured and 41 tag SNPs within eight genes were genotyped.Associations between SNPs and IFX levels were analysed.Then,a multivariate logistic-regression model was developed to predict whether the patients’IFX levels achieved the threshold of therapy(3 lg/mL).Results:Six SNPs(rs7587051,rs143063741,rs442905,rs59457695,rs3213448,and rs3021094)were significantly associated with the post-induction IFX trough level(P=0.015,P<0.001,P=0.046,P=0.022,P=0.011,P=0.013,respectively).A multivariate prediction model of the IFX level was established by baseline albumin(P=0.002),rs442905(P=0.025),rs59457695(P=0.049),rs3213448(P=0.056),and rs3021094(P=0.047).The area under the receiver operating characteristic curve(AUROC)of this prediction model in a representative training dataset was 0.758.This result was verified in a representative testing dataset,with an AUROC of 0.733.Conclusions:Polymorphisms in C1orf106,IL1RN,and IL10 play an important role in the variability of IFX post-induction levels,as indicated in this multivariate prediction model of IFX levels with fair performance.展开更多
Metal ions are essential to the structure and physiological functions of bacteriorhodopsin.Experimental evidence suggests the existence of specific cation binding to the negatively charged groups of Asp85 and Asp212 v...Metal ions are essential to the structure and physiological functions of bacteriorhodopsin.Experimental evidence suggests the existence of specific cation binding to the negatively charged groups of Asp85 and Asp212 via an electrostatic interaction.However,only using electrostatic force is not enough to explain the role of the metal cations because the carboxylate of Asp85 is well known to be protonated in the M intermediate.Considering the presence of some aromatic amino acid residues in the vicinity of the retinal pocket,the existence of cation-πinteractions between the metal cation and aromatic amino acid residues is suggested.Obviously,introduction of this kind of interaction is conducive to understanding the effects of the metal cations and aromatic amino acid residues inside the protein on the structural stability and proton pumping of bacteriorhodopsin.展开更多
基金Project supported by the Foundation of State Key Laboratory of China(Grant Nos.SKLIPR1903Z,1803)the National Natural Science Foundation of China(Grant Nos.U2167208 and 11875223).
文摘Displacement damage effects on the charge-coupled device(CCD)induced by neutrons at the back-streaming white neutron source(Back-n)in the China Spallation Neutron Source(CSNS)are analyzed according to an online irradiation experiment.The hot pixels,random telegraph signal(RTS),mean dark signal,dark current and dark signal non-uniformity(DSNU)induced by Back-n are presented.The dark current is calculated according to the mean dark signal at various integration times.The single-particle displacement damage and transient response are also observed based on the online measurement data.The trends of hot pixels,mean dark signal,DSNU and RTS degradation are related to the integration time and irradiation fluence.The mean dark signal,dark current and DSNU2 are nearly linear with neutron irradiation fluence when nearly all the pixels do not reach saturation.In addition,the mechanisms of the displacement damage effects on the CCD are demonstrated by combining the experimental results and technology computer-aided design(TCAD)simulation.Radiation-induced traps in the space charge region of the CCD will act as generation/recombination centers of electron-hole pairs,leading to an increase in the dark signal.
基金Supported by Nanjing Military Region Innovation Project,No.15MS108and the Youth Nursery Fund,No.18Y024.
文摘BACKGROUND We present a case of focal lymphoblastic transformation to erythroid leukemia following acute myeloblastic transformation in a patient with chronic myelogenous leukemia(CML)and discuss its mechanism of occurrence and development.CASE SUMMARY The presence of the Philadelphia(Ph)chromosome was identified through karyotype analysis,while the BCR-ABL fusion gene was detected using quantitative real-time polymerase chain reaction of the peripheral blood sample.Fluorescence in situ hybridization was used to detect the expression of the BCRABL gene in the lymphoma.Antigen expression and gene mutations in the primitive cells were detected by flow cytometry.The analysis confirmed the presence of CML along with focal lymphoblastic transformation to erythroid leukemia.Additionally,the patient was found to have secondary erythroid leukemia,along with multiple new gene mutations and abnormalities in complex karyotypes of chromosomes.CONCLUSION Our findings suggest a possible molecular basis for the focal lymphoblastic transformation secondary to myeloblastic transformation in patients with CML.
文摘On the basis of interpretation of comprehensive geophysical data and foreign data analysis,there existed a lot of overburden detachment shear thrust faults along the southeastern margin of Nansha Trough,which composed imbricated overthrust nappe structure.Thrust-faulted nappe structure pattern is determined in this area,which consists of frontal fault zone,thrust fault-folded zone and root zone structures,and presents regularly zonation on plane.The detail description of the structural geometrical characteristics is given in shallow thrust fault zone,and the kinematical mechanism of thrust fault nappe structure is furtherly discussed.Overthrnst nappe structure in this area is resulted from island arc-continent collision and orogenic activities.
基金supported by the National Natural Science Foundation of China[Grant No.81573507,81473283,81173131,and 81320108027]the Natural Major Projects for science and technology development from Science and Technology Ministry of China[Grant No.2012ZX09506001-004]+1 种基金the Major Scientific and Technological Project of Guangdong Province[Grant No.2011A080300001]the Medical Scientific Research Foundation of Guangdong Province of China[Grant No.A2020123].
文摘Background:Infliximab(IFX)is the first-line treatment for patients with Crohn’s disease(CD)and is noted for its relatively high cost.The therapeutic efficacy of IFX has noticeable individual differences.Known single-gene polymorphisms(SNPs)are inadequate for predicting non-response to IFX.In this study,we aimed to identify new genetic factors associated with IFX-therapy failure and to predict non-response to IFX by developing a multivariate predictive model.Methods:In this retrospective study,we collected and analysed the data of Chinese patients with CD who received IFX therapy at one hospital between June 2013 and June 2019.Primary non-response(PNR)and non-durable response(NDR)were evaluated using a simple endoscopic score for CD(SES-CD).A total of 125 SNPs within 44 genes were genotyped.A multivariate logistic-regression model was established to predict non-response to IFX.An area-under-the-receiver-operatingcharacteristics curve(AUROC)was applied to evaluate the predictive model performance.Results:Forty-two of 206(20.4%)patients experienced PNR and 15 of 159(9.4%)patients experienced NDR.Nine SNPs were associated with PNR(P<0.05).A PNR predictive model was established,incorporating 2-week high-sensitivity C-reactive protein(hs-CRP),rs61886887,rs61740234,rs357291,rs2269330,and rs111504845,and the AUROC on training and testing data sets were 0.818(P<0.001)and 0.888(P<0.001),respectively.At week 14,hs-CRP levels≥2.25 mg/L were significantly associated with NDR(AUROC=0.815,P<0.001).PNR-associated SNPs were not mutually associated with NDR,suggesting distinct mechanisms between PNR and NDR.Conclusion:Genetic polymorphisms are significantly associated with response to IFX among Chinese CD patients.
基金Supported by the Foundation of State Key Laboratory of Transient Optics and Technology under Grant No.YAK9705the President Foundation of Chinese Academy of Sciences under Grant No.40007059.
文摘A thin oriented bacteriorhodopsin(bR)film is deposited on a stainless steel slide by use of the electrophoretic sedimentation method.A junction is made with electrolyte gels having a counterelectrode to construct a bR-based photoelectric detector.The photoelectric response signal to a 10ns laser pulse is measured.A theory on the photoelectric kinetics of bR is developed based on the concept of the charge displacement current and the bR photocycle rate equations.Comparison between the theoretical and experimental results proves that the bR photoelectric response to a short laser pulse is a multi-exponential process.The decay time constants and amplitudes of each exponential component are obtained by data fitting.
基金funded by grants from the National Natural Science Foundation of China[Grant No.81573507]the National Natural Science Foundation of China[Grant No.81473283]+3 种基金the National Natural Science Foundation of China[Grant No.81173131]the National Natural Science Foundation of China[Grant No.81320108027]the Natural Major Projects for Science and Technology Development from Science and Technology Ministry of China[Grant No.2012ZX09506001-004]the Major Scientific and Technological Project of Guangdong Province,China[Grant No.2011A080300001].
文摘Background:Trough levels of the post-induction serum infliximab(IFX)are associated with short-term and long-term responses of Crohn’s disease patients to IFX,but the inter-individual differences are large.We aimed to elucidate whether single gene polymorphisms(SNPs)within FCGR3A,ATG16L1,C1orf106,OSM,OSMR,NF-jB1,IL1RN,and IL10 partially account for these differences and employed a multivariate regression model to predict patients’post-induction IFX levels.Methods:The retrospective study included 189 Crohn’s disease patients undergoing IFX therapy.Post-induction IFX levels were measured and 41 tag SNPs within eight genes were genotyped.Associations between SNPs and IFX levels were analysed.Then,a multivariate logistic-regression model was developed to predict whether the patients’IFX levels achieved the threshold of therapy(3 lg/mL).Results:Six SNPs(rs7587051,rs143063741,rs442905,rs59457695,rs3213448,and rs3021094)were significantly associated with the post-induction IFX trough level(P=0.015,P<0.001,P=0.046,P=0.022,P=0.011,P=0.013,respectively).A multivariate prediction model of the IFX level was established by baseline albumin(P=0.002),rs442905(P=0.025),rs59457695(P=0.049),rs3213448(P=0.056),and rs3021094(P=0.047).The area under the receiver operating characteristic curve(AUROC)of this prediction model in a representative training dataset was 0.758.This result was verified in a representative testing dataset,with an AUROC of 0.733.Conclusions:Polymorphisms in C1orf106,IL1RN,and IL10 play an important role in the variability of IFX post-induction levels,as indicated in this multivariate prediction model of IFX levels with fair performance.
基金Supported by the National Natural Science Foundation of China under Grant No.69731010the Key Program from Chinese Academy of Sciences under Grant Nos.Kj951-Al-501-05 and Kj952-Sl-03.
文摘Metal ions are essential to the structure and physiological functions of bacteriorhodopsin.Experimental evidence suggests the existence of specific cation binding to the negatively charged groups of Asp85 and Asp212 via an electrostatic interaction.However,only using electrostatic force is not enough to explain the role of the metal cations because the carboxylate of Asp85 is well known to be protonated in the M intermediate.Considering the presence of some aromatic amino acid residues in the vicinity of the retinal pocket,the existence of cation-πinteractions between the metal cation and aromatic amino acid residues is suggested.Obviously,introduction of this kind of interaction is conducive to understanding the effects of the metal cations and aromatic amino acid residues inside the protein on the structural stability and proton pumping of bacteriorhodopsin.
