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上海市基层医护人员对流感及流感疫苗知识认知的情况调查 被引量:13
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作者 张亚男 胡小英 +6 位作者 张锋英 杭晶卿 李凡 程克文 施劲东 瞿介明 揭志军 《中华全科医师杂志》 2020年第8期704-709,共6页
目的:了解上海市基层医护人员对流感及疫苗相应知识的认知、接种流感疫苗的情况及其对亲人/朋友或患者的推荐意愿和影响因素,为提高流感疫苗接种率提供帮助和依据。方法:2017年12月至2018年8月依托上海基层呼吸联盟对上海市各区的中心... 目的:了解上海市基层医护人员对流感及疫苗相应知识的认知、接种流感疫苗的情况及其对亲人/朋友或患者的推荐意愿和影响因素,为提高流感疫苗接种率提供帮助和依据。方法:2017年12月至2018年8月依托上海基层呼吸联盟对上海市各区的中心医院、社区医院的医护人员发放网上问卷1650份,自愿填写。问卷内容包括5个方面:答者的基本信息,流感及疫苗相关知识,接种流感疫苗情况,推荐意愿、态度、看法及影响因素,对流感疫苗宣传的建议。结果:回答有效问卷的1542名医护人员中,88.3%(1361/1542)能正确认识流感的主要症状;58.2%(898/1542)忽略了流感的接触传播途径;41.6%(641/1542)不知道流感疫苗的正确接种次数;82.7%(1276/1542)未认识到孕妇是优先推荐接种疫苗的人群之一。31.2%(481/1542)的医护人员自诉接种过流感疫苗,但社区医院医护人员的接种比例显著高于中心医院[39.1%(304/778)比23.2%(177/764),χ^2=45.44,P<0.05]。医护人员不推荐接种的主要原因有:认为流感抗原易变,接种意义不大[49.5%(404/816)];对流感疫苗效果不确定[48.8%(634/1298)];疫苗是自费的[46.5%(604/1298)]。被调查者认为通过国家制定相关疫苗接种政策[79.7%(1229/1542)]、依靠居委会定期向居民宣教[65.8%(1015/1542)]、一线医护人员对患者推荐[64.4%(993/1542)]是提高流感疫苗接种率的主要途径。结论:上海市基层医护人员对流感和流感疫苗知识的认知仍不足;接种流感疫苗的比例较低,社区医院医护人员的接种率高于中心医院。医护人员推荐流感疫苗接种的意愿受到一些因素的影响,提高医护人员的推荐意愿有助于提高社区居民的接种率。 展开更多
关键词 流感疫苗 接种 认知 医护人员
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Matrix porosity calculation in volcanic and dolomite reservoirs and its application 被引量:7
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作者 Li Ning Wu Hongliang +4 位作者 Feng Qingfu Wang kewen shi Yujiang Li Qingfeng Luo Xinping 《Applied Geophysics》 SCIE CSCD 2009年第3期287-298,301,共13页
Matrix porosity calculations of fractured and vuggy reservoirs, such as volcanics and weathered dolomite, are one of the problems urgently needed to solve in well-log evaluation. In this paper, we first compare the an... Matrix porosity calculations of fractured and vuggy reservoirs, such as volcanics and weathered dolomite, are one of the problems urgently needed to solve in well-log evaluation. In this paper, we first compare the an empirical formula for porosity calculation from full diameter rhyolite core experiments with the matrix porosity formulas commonly used. We discuss the applicability of the empirical formula in fractured and vuggy reservoirs, such as intermediate-basic volcanics and weathered dolomite. Based on core analysis data, the error distribution of the calculated porosity of our empirical formula and the other porosity formulas in these reservoirs are given. The statistical error analysis indicates that the our empirical formula provides a higher precision than the other porosity formulas. When the porosity is between 1.5% and 15%, the acoustic experiment formula can be used not only for acidic volcanics but also in other fractured and vuggy reservoirs, such as intermediate-basic volcanics and weathered dolomite. Moreover, the formula can reduce the effects of borehole enlargement and rock alteration on porosity computation. 展开更多
关键词 POROSITY acoustic slowness VOLCANICS CARBONATES
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近红外光谱结合模式识别算法溯源识别卷烟纸油污 被引量:7
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作者 赵科文 陈实 +5 位作者 尹中尉 邱昌桂 刘静 朱叶梅 周文忠 杨盼盼 《中国测试》 CAS 北大核心 2020年第6期51-55,共5页
为实现对油渍污染卷烟的快速识别及溯源。采集卷烟生产过程中使用的6种润滑油污染在4种卷烟纸上样品的近红外光谱,结合未污染样品的近红外光谱,优选出原始样品原始光谱及二阶导数光谱中共有的差异性光谱波段范围,采用模式识别算法(princ... 为实现对油渍污染卷烟的快速识别及溯源。采集卷烟生产过程中使用的6种润滑油污染在4种卷烟纸上样品的近红外光谱,结合未污染样品的近红外光谱,优选出原始样品原始光谱及二阶导数光谱中共有的差异性光谱波段范围,采用模式识别算法(principal component analysis-mahalanobis distance, PCA-MD)分别建立污染及未污染样品模式识别模型和不同卷烟上6种润滑油的模式识别模型。结果表明:1)基于污染及未污染样品原始近红外光谱及二阶求导光谱差异性分析,优化出的模式识别模型建模波数范围是:6 000~5 300 cm^–1和4 500~4 000 cm^–1;2)建立的污染及未污染模式识别模型前3个主成分累计得分贡献率97.826%,模型分类效果明显,建模集及外部验证集样品的识别准确率均为100%;3)分别建立的6种油渍在4种卷烟纸上的溯源类模型,前3个主成分得分累计贡献率均大于96%,模型分类效果明显,建模集及外部验证集样品的识别准确率均为100%。所建立的基于近红外光谱分析方法结合模式算法(PCA-MD)可实现卷烟生产过程出现的"黄斑烟"中油渍烟的快速识别及污染油渍溯源。 展开更多
关键词 近红外光谱 卷烟 润滑油污染 模式识别
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半滑舌鳎生物钟相关基因克隆及在卵巢发育成熟过程中表达特征分析
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作者 晏科文 马晓东 +3 位作者 史宝 程汉良 王重女 赵新宇 《渔业科学进展》 CSCD 北大核心 2024年第3期101-116,共16页
为阐明生物钟相关基因在半滑舌鳎(Cynoglossus semilaevis)卵巢成熟过程中的作用及机制,采集性成熟半滑舌鳎不同发育期的卵巢组织提取总RNA,通过反转录聚合酶链式反应(RT-PCR)技术对5个核心生物钟基因Clock1a、Bmal1a、Cry1a、Cry2和Per... 为阐明生物钟相关基因在半滑舌鳎(Cynoglossus semilaevis)卵巢成熟过程中的作用及机制,采集性成熟半滑舌鳎不同发育期的卵巢组织提取总RNA,通过反转录聚合酶链式反应(RT-PCR)技术对5个核心生物钟基因Clock1a、Bmal1a、Cry1a、Cry2和Per2的编码区(CDS)序列进行克隆和序列分析。结果显示,Clock1a的CDS序列编码712个氨基酸,序列中有功能性结构域PASD1和PAS11。Bmal1a的CDS序列编码626个氨基酸,序列中有功能性结构域PASD3和PAS11。Cry1a和Cry2的CDS序列分别编码631个、669个氨基酸,序列中均有功能性结构域FAD7。Per2的CDS序列编码1415个氨基酸,序列中有功能性结构域PeriodC和PAS11。通过实时荧光定量PCR(qRT-PCR)分析5个生物钟基因mRNA在性成熟半滑舌鳎卵巢不同发育期的表达特性,发现5个生物钟基因存在性腺发育周年表达规律,且在卵巢Ⅱ期和Ⅲ期相对高表达(P<0.05),在卵巢Ⅳ、Ⅴ和Ⅵ期相对低表达,这说明生物钟基因对半滑舌鳎的卵巢发育成熟起到重要的调节作用。研究结果为进一步探究生物钟基因调控半滑舌鳎卵巢发育成熟的机制提供了重要参考,为提高半滑舌鳎的繁育效率提供了理论基础。 展开更多
关键词 生物钟基因 半滑舌鳎 卵巢 周年表达规律
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基于数据挖掘探讨云南姚氏妇科论治月经后期用药规律
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作者 陈珂雯 罗佳 +4 位作者 石丽琼 徐涟 林莉 陈静 高春泽 《中国民族民间医药》 2024年第15期94-98,共5页
目的:记录和整理2022年云南姚氏医学流派妇科主要传承人论治月经后期的医案,探究姚氏妇科治疗月经后期的用药规律。方法:运用中医药传承平台V2.5进行频数和关联规则分析。结果:纳入并筛选出医案172例,治疗月经后期共156方,中药频次统计... 目的:记录和整理2022年云南姚氏医学流派妇科主要传承人论治月经后期的医案,探究姚氏妇科治疗月经后期的用药规律。方法:运用中医药传承平台V2.5进行频数和关联规则分析。结果:纳入并筛选出医案172例,治疗月经后期共156方,中药频次统计为148种,高频中药50种,以甘草、当归、白芍、白术等为主,归经以肝肾脾三经最多,得出药对关联规则和聚类分析药组为甘草-当归-白芍等。结论:姚氏妇科论治月经后期疾病注重肝肾脾关系,强调气血的重要性,善用经方,用药特色鲜明,值得进一步探讨。 展开更多
关键词 姚氏妇科 月经后期 数据挖掘 用药规律
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几何非线性条件下微纳米梁型谐振器的非线性支撑损失
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作者 马程中 王检耀 +4 位作者 史柯文 孔子文 杨伟东 陈思宇 国凤林 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2024年第3期165-175,共11页
作为微纳米机械谐振器主要的能量耗散源之一,支撑损失主要采用未考虑中面拉伸效应的线性理论进行研究.然而该理论却无法适用于大挠度非线性振动状态谐振器的支撑损失.为此,我们研究了考虑几何非线性和轴向预拉力的两端固支微梁谐振器处... 作为微纳米机械谐振器主要的能量耗散源之一,支撑损失主要采用未考虑中面拉伸效应的线性理论进行研究.然而该理论却无法适用于大挠度非线性振动状态谐振器的支撑损失.为此,我们研究了考虑几何非线性和轴向预拉力的两端固支微梁谐振器处于面内弯曲振动模式时的支撑损失,旨在准确模拟大挠度振动下高性能谐振器的支撑损失.本文不仅考虑了支撑结构与谐振器连接区域受中面拉伸产生的正应力作用而引起的支撑损失,也考虑了受剪应力作用而引起的支撑损失,并且理论结果与有限元模拟以及文献中实验结果一致.另外,本文还研究了轴向预拉力对谐振器非线性振动特性的影响,并讨论了谐振器的振幅、长高比以及轴向预拉力对支撑损失的影响.理论结果表明,相比于线性振动状态下谐振器的支撑损失,由谐振器大变形振动产生的中面拉伸效应会引起更大的支撑损失,并且会随非线性振幅的增大而增大.本文提出的非线性支撑损失模型有望对评估非线性振动谐振器的支撑损失以及阐明支撑损失随非线性振幅增大的现象提供理论指导. 展开更多
关键词 Support loss Micro/nano beam resonator Geometric nonlinearity Axial pre-tension
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合肥市再生水利用的发展战略研究 被引量:4
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作者 王柯文 吴凤平 石林 《湖南水利水电》 2021年第1期101-105,共5页
再生水是国际公认的“第二水源”,是解决城市水资源短缺的重要举措之一。文章通过查阅资料和实地调研,分析合肥市再生水资源的利用现状,结合合肥市再生水利用的优势与劣势、面临的机遇与挑战,构建战略矩阵,针对性提出再生水利用的推广策... 再生水是国际公认的“第二水源”,是解决城市水资源短缺的重要举措之一。文章通过查阅资料和实地调研,分析合肥市再生水资源的利用现状,结合合肥市再生水利用的优势与劣势、面临的机遇与挑战,构建战略矩阵,针对性提出再生水利用的推广策略,为当地推动再生水利用提供参考。 展开更多
关键词 再生水 节水 SWOT分析 合肥市
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Spintronics intelligent devices
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作者 Wenlong Cai Yan Huang +5 位作者 Xueying Zhang shihong Wang Yuanhao Pan Jialiang Yin kewen shi Weisheng Zhao 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2023年第11期17-43,共27页
Intelligent computing paradigms have become increasingly important for the efficient processing of massive amounts of data.However,using traditional electronic devices to implement these intelligent paradigms is curre... Intelligent computing paradigms have become increasingly important for the efficient processing of massive amounts of data.However,using traditional electronic devices to implement these intelligent paradigms is currently mismatched and limited by their energy,area,and speed.Spintronics,which exploits the magnetic and electrical properties of electrons,could break through these limitations and bring new possibilities to electrical devices.In particular,the tunneling magnetoresistance effect,merging quantum and spintronics,enables spintronic devices to be compatible with standard integrated circuits with a magnetic tunnel junction(MTJ)design,showing great potential for implementing hardware-based intelligent frameworks.In this review,we introduce the specific capabilities of MTJs,including nonvolatility,stochasticity,plasticity,and nonlinearity,which are highly favorable in artificial intelligence algorithms.We then present how these devices could impact the development of intelligent computing,including in-memory computing,probabilistic computing,and neuromorphic computing.Finally,we discuss their challenges and perspectives in intelligent hardware implementations. 展开更多
关键词 tunneling magnetoresistance effect magnetic tunnel junction in-memory computing probabilistic computing neuromorphic computing
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Dyeing Thermodynamics and Supramolecular Structure of Lac Red on Protein Fibers
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作者 kewen Fu Jian Li +6 位作者 Dongkai Qin Luwei shi Xiangyu Ni Kaikai Zhao Dequan Xu Ailin Yuan Chunling Zheng 《Journal of Textile Science and Technology》 2022年第2期89-106,共18页
The dyeing temperature of natural dye lac red on two kinds of natural protein fibers was studied, and the interaction between dyestuff and fiber was discussed through thermodynamic study and density functional theory ... The dyeing temperature of natural dye lac red on two kinds of natural protein fibers was studied, and the interaction between dyestuff and fiber was discussed through thermodynamic study and density functional theory (DFT) calculation. The optimum temperature for lac red dyed silk was 60˚C and wool showed a better response at 90˚C. The thermodynamics study revealed good Nernst isotherm and Freundlich adsorption models respectively, and the lac dye adsorption processes were both spontaneous and exothermic. The potential interaction of Laccaic acid A with the external environment by electrostatic potential and atomic charge distribution was first explored. With molecular simulation, Laccaic acid A and glycine composed 8 stable complexes. Then, typical hydrogen bonds, bond length, and binding energy, etc. were analyzed. The results revealed lac red on silk and wool fabric mainly depended on the weak hydrogen bonds and van der Waals force which determined the low dye fastness. 展开更多
关键词 Natural Dye Lac Red WOOL SILK Dyeing Thermodynamics DFT Calculation WAVEFUNCTION
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Antiferromagnetic spintronics: An overview and outlook 被引量:1
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作者 Danrong Xionga Yuhao Jiang +9 位作者 kewen shi Ao Du Yuxuan Yao Zongxia Guo Daoqian Zhu Kaihua Cao Shouzhong Peng Wenlong Cai Dapeng Zhu Weisheng Zhao 《Fundamental Research》 CAS 2022年第4期522-534,共13页
Over the pas few decades,the diversified development of antiferomagnetic spintronics has made antiferomagnets(AFMs)interesting and very useful.After tough challenges,the applications of AFMs in electronic devices have... Over the pas few decades,the diversified development of antiferomagnetic spintronics has made antiferomagnets(AFMs)interesting and very useful.After tough challenges,the applications of AFMs in electronic devices have transitioned from focusing on the interface coupling features to achieving the manipulation and detection of AFMs.As AFMs are internally magnetic,taking full use of AFMs for information storage has been the main target of research.In this paper,we provide a comprehensive description of AFM spintronics applications from the interface coupling,read-out operations,and writing manipulations perspective.We examine the early use of AFMs in magnetic recordings and conventional magnetoresistive random-access memory(MRAM),and review the latest mechanisms of the manipulation and detection of AFMs.Finally,based on exchange bias(EB)manipulation,a high-performance EB-MRAM is introduced as the next generation of AFM-based memories,which provides an effective method for read-out and writing of AFMs and opens a new era for AFM spintronics. 展开更多
关键词 ANTIFERROMAGNETS MRAM SPINTRONICS Spin-orbit torque Exchange bias
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混种牛膝菊嫁接后代对生菜镉积累的影响 被引量:2
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作者 黄科文 林立金 +3 位作者 石军 马倩倩 李克强 廖明安 《土壤》 CAS CSCD 北大核心 2020年第1期127-133,共7页
采用盆栽试验,将两种生态型(农田和矿山)镉超富集植物牛膝菊进行正反嫁接:以矿山生态型牛膝菊为砧木(后代为矿砧)、农田生态型牛膝菊为接穗(后代为农穗)进行嫁接和以农田生态型牛膝菊为砧木(后代为农砧)、矿山生态型牛膝菊为接穗(后代... 采用盆栽试验,将两种生态型(农田和矿山)镉超富集植物牛膝菊进行正反嫁接:以矿山生态型牛膝菊为砧木(后代为矿砧)、农田生态型牛膝菊为接穗(后代为农穗)进行嫁接和以农田生态型牛膝菊为砧木(后代为农砧)、矿山生态型牛膝菊为接穗(后代为矿穗)进行嫁接,研究了混种两种生态型牛膝菊正反嫁接实生后代(农田、矿山、农砧、矿砧、农穗、矿穗)对生菜镉(Cd)积累的影响。结果表明:与生菜单种处理相比,只有混种农穗及矿穗处理不同程度提高了生菜根系及地上部分的生物量(其中,混种矿穗处理的生菜地上部分生物量最大,较生菜单种处理提高了10.98%),其他混种处理均抑制了生菜的生长。另外,混种农穗及矿穗处理显著提高了生菜叶片过氧化物酶(POD)及过氧化氢酶(CAT)活性,但对其超氧化物歧化酶(SOD)活性的影响不大。混种牛膝菊嫁接后代显著降低了生菜地上部分的Cd含量,且与矿穗混种处理的Cd含量最低,较生菜单种处理降低了29.18%。因此,生菜与矿穗混种,既能有效降低生菜可食用部位(地上部分)的Cd含量,还能促进其生长。 展开更多
关键词 牛膝菊 嫁接后代 生菜 混种
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Mechanism of field-like torque in spin-orbit torque switching of perpendicular magnetic tunnel junction 被引量:1
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作者 Yudong Zhuo Wenlong Cai +7 位作者 Daoqian Zhu Hongchao Zhang Ao Du Kaihua Cao Jialiang Yin Yan Huang kewen shi Weisheng Zhao 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2022年第10期109-114,共6页
The current-induced spin-orbit torque(SOT) is one of the most promising ways for high speed and low power spintronics devices. However, the mechanism of SOT driven magnetization reversal, especially the role of the fi... The current-induced spin-orbit torque(SOT) is one of the most promising ways for high speed and low power spintronics devices. However, the mechanism of SOT driven magnetization reversal, especially the role of the field-like torque(FLT), is still unclear. Here, we report the observed promotion and suppression of switching by FLT, which depends on the relative direction of FLT and spin polarization. Our results reveal that the FLT could modulate the switching speed and power consumption by affecting the work done by the damping-like torque, and leads two different reversal dynamical paths during the switching.Furthermore, the origin of incubation time in SOT induced switching is clarified simultaneously. 展开更多
关键词 spin-orbit torque field-like torque magnetization reversal magnetic tunnel junction
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Application of gas wettability alteration to improve methane drainage performance:A case study 被引量:2
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作者 Lin Jia kewen Li +2 位作者 Xiaohong shi Lipeng Zhao Jianshe Linghu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2021年第4期621-629,共9页
Hydraulic fracturing technique is widely used for methane drainage and has achieved good effects in numerous coal mines,but negative effects may occur as the fracturing fluids are absorbed into the coal seam.Gas wetta... Hydraulic fracturing technique is widely used for methane drainage and has achieved good effects in numerous coal mines,but negative effects may occur as the fracturing fluids are absorbed into the coal seam.Gas wettability alteration(GWA)technology can be used as it can enhance the gas and water mobility during dewatering process as a result of capillary pressure change.However,there have been few reported field tests in coal mines using GWA technology.This paper describes a pilot-scale field test in Xinjing coal mine,Yangquan,China.The fluorocarbon surfactants perfluorooctyl methacrylate monomer-containing polymethacrylate(PMP)was used to alter the wettability of coal seam to strong gas-wetness during the hydraulic fracturing process.The study focuses on the comparison of two boreholes(Boreholes#9 and#10)and one other borehole(Borehole#8)with and without using GWA approach.A well-defined monitoring program was established by measuring the dewatering volume of the fracturing fluid and the drainage volume of methane as well as the concentration.The field test results showed that the average methane drainage rates of Boreholes#9(39.28 m^(3)/d)and#10(51.04 m^(3)/d)with GWA treatment exceeded that of Borehole#8(21.09 m^(3)/d)without GWA treatment,with an increase of 86.3%and 142.1%,respectively.The average methane concentrations of Boreholes#9(4.05%)and#10(6.18%)were 64.6%and 151.2%higher than that of Borehole#8(2.46%),respectively.On the other hand,the dewatering ratio of Boreholes#9(4.36%)and#10(3.11%)was almost 19 times and 13 times greater than that of Borehole#8(0.22%).These field test results were in agreement with the experimental data.The significant increase in both methane concentration and dewatering ratio demonstrated that GWA technology could be applied for enhanced methane drainage in coal mines.Important lessons learned at Xinjing coal mine might be applied to other coal mines in China and elsewhere. 展开更多
关键词 Field test Hydraulic fracturing Wettability alteration to gas-wetness Drainage of methane
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Observation of magnetic droplets in magnetic tunnel junctions
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作者 kewen shi Wenlong Cai +10 位作者 Sheng Jiang Daoqian Zhu Kaihua Cao Zongxia Guo Jiaqi Wei Ao Du Zhi Li Yan Huang Jialiang Yin Johan Akerman Weisheng Zhao 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2022年第2期99-105,共7页
Magnetic droplets,a class of highly nonlinear magnetodynamic solitons,can be nucleated and stabilized in nanocontact spintorque nano-oscillators.Here we experimentally demonstrate magnetic droplets in magnetic tunnel ... Magnetic droplets,a class of highly nonlinear magnetodynamic solitons,can be nucleated and stabilized in nanocontact spintorque nano-oscillators.Here we experimentally demonstrate magnetic droplets in magnetic tunnel junctions(MTJs).The droplet nucleation is accompanied by power enhancement compared with its ferromagnetic resonance modes.The nucleation and stabilization of droplets are ascribed to the double-Co Fe B free-layer structure in the all-perpendicular MTJ,which provides a low Zhang-Li torque and a high pinning field.Our results enable better electrical sensitivity in fundamental studies of droplets and show that the droplets can be utilized in MTJ-based applications and materials science. 展开更多
关键词 spin-torque nano-oscillators magnetic droplets spin dynamics magnetic tunnel junctions
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A COMPARATIVE RESEARCH OF ZYMOGRAMS OF LACTATE DEHYDROGENASE IN DIFFERENT TISSUES 0F 25 SPECIES OF VERTEBRATES
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作者 Chen Fengying (1) Zeng kewen (1) +1 位作者 Pan shi (1) Yang Mingfei (1) 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1991年第1期56-64,共9页
The distribution and characteristics of LDH isoenzymes in different tissues of 25spe-cies of vertebrats were comparativety analysed by discontinuous polyacrylamide gel diskelectrophoresis,combined with experiments on ... The distribution and characteristics of LDH isoenzymes in different tissues of 25spe-cies of vertebrats were comparativety analysed by discontinuous polyacrylamide gel diskelectrophoresis,combined with experiments on heat and urea inhibition.The result showed that indifferent tissues of Cienopharyngodon idellus,Carassius,ayratus,Trichiurus haumete and Miichihysmiiuy in addition to the distribution of LDH isoenzymes,composed of A and B subuits,a E4isoenzyme band,moving very fast to the positive pole,was detectedin the eye tissue of Trichiurushaumele and a F4 isoenzyme hand,moving very slow to the positive pole,was found in liver andkidney of Cienopharyngodon idellus.The A4 isoenzyme band was found in different tisseues ofPleuronichlhys cornulus,inhabiting on the bottom of the ocean.From the amphition to the mammal,with the exception of Hyta arboreu immaculata,Chinnemys reevesii,Trioyx sinensis,Gallus gallusdomeslicus and Corvus macrorhynchos,which have special electrophoretic behaviours.In 展开更多
关键词 VERTEBRATE POLYACRYLAMIDE GEL DISK elcctrophorcsis LDH isocnzyme Tissue SPECIFICITY
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基于TLR4-Ig融合蛋白探究TLR4信号在溃疡性结肠炎中的关键作用
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作者 钱珂文 王楚棋 +7 位作者 张淑怡 李光耀 邹宜覃 郑欣亚 艾泓如 傅文燕 雷长海 胡适 《海军军医大学学报》 CAS CSCD 北大核心 2024年第5期535-543,共9页
目的探究Toll样受体4(TLR4)信号与溃疡性结肠炎(UC)发生、发展的关系。方法收集UC患者结直肠活检组织样本(n=63),根据Matts组织病理学分级标准分为1~5级,利用免疫组织化学染色检测TLR4表达情况。利用FreeStyle 293表达系统制备TLR4-Ig... 目的探究Toll样受体4(TLR4)信号与溃疡性结肠炎(UC)发生、发展的关系。方法收集UC患者结直肠活检组织样本(n=63),根据Matts组织病理学分级标准分为1~5级,利用免疫组织化学染色检测TLR4表达情况。利用FreeStyle 293表达系统制备TLR4-Ig融合蛋白。体外培养健康者来源的外周血单个核细胞(PBMC),在培养基中添加100μg/mL脂多糖诱导炎症模型,并加入100μg/mL TLR4-Ig阻断TLR4,检测粒细胞-巨噬细胞集落刺激因子、干扰素γ、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10和TNF-α的分泌水平。以葡聚糖硫酸钠(DSS)诱导小鼠急性结肠炎模型,分别采用20 mg/kg TLR4-Ig、40 mg/kg TLR4-Ig、抗生素+40 mg/kg TLR4-Ig进行干预,取小鼠结肠组织测量结肠长度并进行组织病理学评估。结果UC患者结肠组织Matts分级越高TLR4高表达的样本比例越高(P<0.001)。TLR4-Ig融合蛋白能够以高亲和力结合TLR4配体,阻断TLR4信号介导的PBMC活化和炎症因子分泌。TLR4-Ig融合蛋白对TLR4信号的阻断加重了DSS诱导的小鼠急性结肠炎,而抗生素处理则对TLR4信号阻断造成的小鼠急性结肠炎加重有缓解作用。结论TLR4信号与肠道菌群的信号交流是UC发生早期的重要保护机制,TLR4信号的缺失不利于缓解急性炎症和修复肠黏膜。 展开更多
关键词 TOLL样受体4 溃疡性结肠炎 抗体融合蛋白 免疫组织化学 葡聚糖硫酸钠
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3种制氢技术路线的经济性分析
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作者 郭可玟 续永杰 史瑞静 《电工技术》 2024年第7期40-43,共4页
为分析不同制氢技术路线的成本,选取了天然气制氢、石油制氢、电解水制氢3种制氢方式来测算制氢成本。其中,天然气的制氢成本最低,石油制氢的成本最高。分析了3种制氢方式的成本组成,发现制氢成本的主要部分都是原料/电力成本。讨论了原... 为分析不同制氢技术路线的成本,选取了天然气制氢、石油制氢、电解水制氢3种制氢方式来测算制氢成本。其中,天然气的制氢成本最低,石油制氢的成本最高。分析了3种制氢方式的成本组成,发现制氢成本的主要部分都是原料/电力成本。讨论了原料/电力成本、设备投资、碳税税率3个变量对氢气价格的影响,得出原料/电力成本对氢气价格的影响远大于设备投资、碳税税率的结论。 展开更多
关键词 天然气制氢 石油制氢 电解水制氢 经济性分析
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