Objective： To study the chemical constituents from the Et OAc extract of endophytic fungal Plectosphaerella cucumerina YCTA2 Z1.Methods： The chemical constituents were isolated and purified by silica gel column,Seph...Objective： To study the chemical constituents from the Et OAc extract of endophytic fungal Plectosphaerella cucumerina YCTA2 Z1.Methods： The chemical constituents were isolated and purified by silica gel column,Sephadex LH-20,and reverse-phase C-18 column chromatography as well as crystallization.Results： Thirteen compounds were isolated from the EtOAc extract of the fungal strain YCTA2 Z1.Their chemical structures were elucidated according to the spectral evidence.They were identified as caudatin（1）,baishouwubenzophenone（2）,cynandione B（3）,asterbatanoside A（4）,p-hydroxyphenethyl-O-β-D-glycoside（5）,caffeic acid（6）,ferulic acid（7）,2,5-dihydroxyacetophenone（8）,protocatechuic acid（9）,vanillic acid（10）,stearic acid（11）,azelaic acid（12）,and succinic acid（13）.Conclusions： It is the first chemical study on endophytic fungi from Cynanchum auriculatum and all the compounds are obtained from the species,the genus,as well as the family Plectosphaerellaceae for the first time.展开更多
Follistatin(FST) is an important regulator of skeletal muscle growth and adipose deposition through its ability to bind to several members of the transforming growth factor-β(TGF-β) superfamily, and thus may be a go...Follistatin(FST) is an important regulator of skeletal muscle growth and adipose deposition through its ability to bind to several members of the transforming growth factor-β(TGF-β) superfamily, and thus may be a good candidate for future animal breeding programs. However, the molecular mechanisms underlying the phenotypic changes have yet to be clarified in pig. We generated transgenic(TG) pigs that express human FST specifically in skeletal muscle tissues and characterized the phenotypic changes compared with the same tissues in wild-type pigs. The TG pigs showed increased skeletal muscle growth, decreased adipose deposition, and improved metabolism status(P<0.05). Transcriptome analysis detected important roles of the PIK3–AKT signaling pathway, calcium-mediated signaling pathway, and amino acid metabolism pathway in FST-induced skeletal muscle hypertrophy, and depot-specific oxidative metabolism changes in psoas major muscle. Furthermore, the lipid metabolism-related process was changed in adipose tissue in the TG pigs. Gene set enrichment analysis revealed that genes related to lipid synthesis, lipid catabolism, and lipid storage were down-regulated(P<0.01) in the TG pigs for subcutaneous fat, whereas genes related to lipid catabolism were significantly up-regulated(P<0.05) in the TG pigs for retroperitoneal fat compared with their expression levels in wild-type pigs. In liver, genes related to the TGF-β signaling pathway were over-represented in the TG pigs, which is consistent with the inhibitory role of FST in regulating TGF-β signaling. Together, these results provide new insights into the molecular mechanisms underlying the phenotypic changes in pig.展开更多
Objective To estimate the clinical and pathological features of pancreatic solid cystic papillary tumor (SCPT) in children. Methods From 2000 to 2005, 8 cases with SCPT of the pancreas were analyzed retrospectively. A...Objective To estimate the clinical and pathological features of pancreatic solid cystic papillary tumor (SCPT) in children. Methods From 2000 to 2005, 8 cases with SCPT of the pancreas were analyzed retrospectively. All cases but one were females. Average age was 12.8 years. By case review, we discussed the clinical and pathological features of SCPT in children. Results The chief complains were abdominal pain and palpable mass. There were 3 cases in the head, 1 case in the body, and 4 cases in the tail of pancreas. The procedures employed included local resection (1 case), distal pancreatectomy (5 cases), pancreaticoduodenectomy (1 case), and biopsy (1 case). Histological examination showed solid with cystic areas and papillary protrusions in the 8 cases; as for immunohistochemical examinations, the positive rate was 100% for α-antitrypsin (AACT), 87.5% for vinmentin, and 62.5% for neuron-specific enolase (NSE). The patients were followed up for 2 months to 4 years but one was lost by follow-up and all were alive postoperatively. SCPT in 2 cases relapsed. Conclusion Occurring predominantly in young females, SCPT is usually curable by surgical resection with a favorable prognosis.展开更多
Chromovitrectomy is a surgical method that uses intraocular dyes during vitreoretinal surgery to stain transparent tissues in the eye such as the posterior vitreous cortex,epiretinal membrane and inner limiting membra...Chromovitrectomy is a surgical method that uses intraocular dyes during vitreoretinal surgery to stain transparent tissues in the eye such as the posterior vitreous cortex,epiretinal membrane and inner limiting membrane for easy operation.It can avoid the intraocular complications associated with incomplete vitrectomy,incomplete epiretinal membrane peeling and incomplete internal limiting membrane peeling.At present,the commonly used vital dyes in clinic are as follows:indocyanine green,infracyanine green,triamcinolone acetonide,trypan blue,brilliant blue G.Indocyanine green was first used for intraocular staining,which can show the inner limiting membrane well.Following indocyanine green,trypan blue is used to identify idiopathic epiretinal membranes and triamcinolone acetonide is used to stain the vitreous.Recently,it has been discovered that natural dyes extracted from fruits,such as lutein and anthocyanins,are also used in intraocular vitrectomy.The main purpose of this review is to explore the research status and prospects of various intraocular stains in stained vitrectomy.展开更多
To investigate the stability of gravity anchors of suspension bridges,in-situ tests of the vertical bearing capacity of the bedrock,shear resistance of the anchor-rock interface,shear resistance of the bedrock were co...To investigate the stability of gravity anchors of suspension bridges,in-situ tests of the vertical bearing capacity of the bedrock,shear resistance of the anchor-rock interface,shear resistance of the bedrock were conducted in a suspension bridge project.Under dry-wet cycles,the deterioration law of the mechanical properties of argillaceous sandstone was identified in laboratory tests:the elastic modulus,cohesion and friction of the argillaceous sandstone deteriorated significantly at first few dry-wet cycles and then declined slowly after 10 cycles,ultimately these three mechanical parameters were reduced to about 1/3,1/3,2/3 of the initial value respectively.Moreover,numerical simulation was used to restore in-situ shear tests and a good agreement was obtained.Base on the results of in-situ and laboratory tests,the stability of the gravity anchor foundation under natural conditions and drywet cycles was calculated and its failure modes were analyzed.The results demonstrated that the dry-wet cycles caused uneven settlement of the anchor foundation,resulting in more serious stress concentration in the substrate.The dry-wet cycles remarkably reduced the stability coefficient of the anchor foundation,whose failure mode shifted from overturning failure under natural conditions to sliding failure.When there was weak interlayer in the rock layer,the anti-sliding stability of the anchor foundation was affected drastically.展开更多
The technology of anti-collision is a key point in radio frequency identification （RFID） system. To avoid data collision, there are two approaches： ALOHA based algorithm and binary tree （BT） based algorithm. Howe...The technology of anti-collision is a key point in radio frequency identification （RFID） system. To avoid data collision, there are two approaches： ALOHA based algorithm and binary tree （BT） based algorithm. However, these cannot solve the collision problem completely, especially when the tag quantity is big and the tag ID is long. In this article, we present a multi-branch query tree （MBQT） protocol based on balanced incomplete block design （BIBD） code, and use 16-bit vectors derived from the BIBD as query prefix symbols of RFID reader. Compared with the conventional anti-collision algorithm, the theoretic analysis and simulation show that the proposed protocol improves the identification efficiency.展开更多
Objective To investigate the diagnosis and therapy of mediastinal enterogenous cysts in children. Methods Clinical data of 17 cases with mediastinal enterogenous cysts within 19 years in our hospital were retrospectiv...Objective To investigate the diagnosis and therapy of mediastinal enterogenous cysts in children. Methods Clinical data of 17 cases with mediastinal enterogenous cysts within 19 years in our hospital were retrospectively analyzed. Results One case was intramural esophageal cyst and 16 cases were enteric cysts, two among which were complicated with abdominal enteric duplications. Most cases presented with symptoms of respiratory distress. Twelve cases were complicated with vertebral anomalies. Ultrasound helpful in confirming the cystic nature of these lesions. posterior mediastinum. of 12 cases and magnetic resonance imaging of 4 cases were Eight cases had technetium-99m pertechnetate scintigraphy of posterior mediastinum.Conclusions Most patients present with symptoms of respiratory distress, complicated with vertebral anomalies. Ultrasonography and magnetic resonance imaging may be helpful in confirming the cystic nature of these lesions. Technetium-99m pertechnetate scintigraphy is the most effective method for differentiation of the disease from other mediastinal cysts.展开更多
The Mg-Al layered double hydroxide(LDH)conversion coatings were first synthesized in situ to modify the AZ91 D alloy through urea hydrolysis to adjust the pH values(9.4,10.4,11.2 and 11.4).The pH 11.2 Mg-Al LDH posses...The Mg-Al layered double hydroxide(LDH)conversion coatings were first synthesized in situ to modify the AZ91 D alloy through urea hydrolysis to adjust the pH values(9.4,10.4,11.2 and 11.4).The pH 11.2 Mg-Al LDH possessed the best compactness and good crystallinity compared to other in situ LDH coatings and obtained the lowest corrosion current density(i_(corr))of(2.884×10^(-6)±0.345×10^(-6))A·cm^(-2),which was attributed to the anion-exchange reaction of LDH and the physical barrier against corrosion owing to the twisted penetration pathway of the interlaced LDH sheets.Core-shell structured Zn-Al LDH@ZIF-8 powder modified with stearic acid(SA)was further wrapped with polyvinylidene fluoride(PVDF)to prepare a hydrophobic double-layered coating on the underlying pH 11.2 Mg-Al LDH(SLLZ).The water contact angle(CA)of the SLLZ coating reached 105.6°,and its i_(corr)decreased to(3.524×10^(-7)±0.214×10^(-7))A·cm^(-2)compared with a single pH 11.2 film.The SLLZ coating exhibited high durability and corrosion protection,even after15 days of immersion in NaCl solution.The PVDF,SA and ZIF-8 nano-shells contributed to good hydrophobicity,effectively forming a physical barrier.The Zn-Al LDH core and underlying in situ Mg-Al LDH were beneficial for synergistically promoting anion-exchange reaction between the intercalated anions of LDH and chlorides in corrosive media.This work provides a promising approach that combines core-shell LDH@ZIF-8 with LDH film on a Mg alloy surface to construct a hydrophobic film with excellent longterm anti-corrosion performance.展开更多
For patients with COVID-19 caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the damages to multiple organs have been clinically observed.Since most of current investigations for virus-host interac...For patients with COVID-19 caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the damages to multiple organs have been clinically observed.Since most of current investigations for virus-host interaction are based on cell level,there is an urgent demand to probe tissue-specific features associated with SARS-CoV-2 infection.Based on collected proteomic datasets from human lung,colon,kidney,liver,and heart,we constructed a virus-receptor network,a virus-interaction network,and a virus-perturbation network.In the tissue-specific networks associated with virus-host crosstalk,both common and different key hubs are revealed in diverse tissues.Ubiquitous hubs in multiple tissues such as BRD4 and RIPK1 would be promising drug targets to rescue multi-organ injury and deal with inflammation.Certain tissue-unique hubs such as REEP5 might mediate specific olfactory dysfunction.The present analysis implies that SARS-CoV-2 could affect multi-targets in diverse host tissues,and the treatment of COVID-19 would be a complex task.展开更多
Indigenous chicken products are increasingly favored by consumers due to their unique meat and egg quality.However,the relatively poor egg-laying performance largely impacts the economic benefits and hinders sustainab...Indigenous chicken products are increasingly favored by consumers due to their unique meat and egg quality.However,the relatively poor egg-laying performance largely impacts the economic benefits and hinders sustainable development of the local chicken industry.Thus,excavating key genes and effective molecular markers associated with egg-laying performance is necessary to improve egg production via genetic selection in indigenous breeds.In the present study,comparative hypothalamic transcriptome between pre-laying(15 weeks old)and peak-laying(30 weeks old)Lushi blueshelled-egg(LBS)chicken was performed.A total of 518 differentially expressed genes(DEGs)were identified.Among the DEGs,64 genes were enriched in 10 Gene Ontology(GO)terms associated with reproductive regulation via GO analysis and considered as potential candidate genes regulating egg-laying performance.Of the 64 genes,16 showed high connectivity(degree≥12)by protein–protein interaction(PPI)network analysis and were considered as potential core candidate genes(PCCGs).To further look for key candidate genes from the PCCGs,firstly,the expression patterns of the 16 genes were examined in the hypothalamus of two indigenous breeds(LBS and Gushi(GS)chickens)between the pre-laying and peak-laying stages using quantitative real-time PCR(qRT-PCR).Eleven out of the 16 genes showed significantly differential expression(P<0.05)with the same changing trends in the two breeds.Then,correlations between the expression levels of the above 11 genes and egg numbers and reproductive hormone concentrations in serum were investigated in high-yielding and low-yielding GS chickens.Of the 11 genes,eight showed significant correlations(P<0.05)between their expression levels and egg numbers,and between expression levels and reproductive hormone concentration in serum.Furthermore,an association study on single nucleotide polymorphisms(SNPs)identified in these eight genes and egg production traits was carried out in 640 GS hens,and a significant association(P<0.05)between the展开更多
RNA can interact with RNA-binding proteins(RBPs),mRNA,or other non-coding RNAs(ncRNAs)to form complex regulatory networks.High-throughput CLIP-seq,degradome-seq,and RNA-RNA interactome sequencing methods represent pow...RNA can interact with RNA-binding proteins(RBPs),mRNA,or other non-coding RNAs(ncRNAs)to form complex regulatory networks.High-throughput CLIP-seq,degradome-seq,and RNA-RNA interactome sequencing methods represent powerful approaches to identify biologically relevant ncRNA-target and protein-ncRNA interactions.However,assigning ncRNAs to their regulatory target genes or interacting RNA-binding proteins(RBPs)remains technically challenging.Chemical modifications to mRNA also play important roles in regulating gene expression.Investigation of the functional roles of these modifications relies highly on the detection methods used.RNA structure is also critical at nearly every step of the RNA life cycle.In this review,we summarize recent advances and limitations in CLIP technologies and discuss the computational challenges of and bioinformatics tools used for decoding the functions and regulatory networks of ncRNAs.We also summarize methods used to detect RNA modifications and to probe RNA structure.展开更多
To improve the spectrum sensing performance in cognitive radios, a scheme of cooperative blind spectrum sensing based on autocorrelation matrix is proposed. The test statistic is extracted from the autocorrelation mat...To improve the spectrum sensing performance in cognitive radios, a scheme of cooperative blind spectrum sensing based on autocorrelation matrix is proposed. The test statistic is extracted from the autocorrelation matrix of the received signal samples and a bi-threshold hybrid decision scheme is designed for local spectrum sensing. The cognitive radio base station makes a credibility fusion based on the local soft decisions and then takes global fusion combining with the local hard decisions. The proposed method is blind in the sense since it requires no apriori knowledge of the signal and the noise power. Theoretical analysis and computer simulation results show that the proposed method can enhance the spectrum sensing capability.展开更多
Accurate classification of EEG left and right hand motor imagery is an important issue in brain-computer interface. Firstly, discrete wavelet transform method was used to decompose the average power of C3 electrode an...Accurate classification of EEG left and right hand motor imagery is an important issue in brain-computer interface. Firstly, discrete wavelet transform method was used to decompose the average power of C3 electrode and C4 electrode in left-right hands imagery movement during some periods of time. The reconstructed signal of approximation coefficient A6 on the 6al level was selected to build up a feature signal. Secondly, the performances by Fisher Linear Discriminant Analysis with two different threshold calculation ways and Support Vector Machine methods were compared. The final classification results showed that false classification rate by Support Vector Machine was lower and gained an ideal classification results.展开更多
基金National Natural Science Foundation of China (21502014,81673017,51408094)Natural Science Foundation of Liaoning Province,China (201602114)Research Foundation of Educational Committee of Liaoning Province,China (JDL2017027)
文摘Objective： To study the chemical constituents from the Et OAc extract of endophytic fungal Plectosphaerella cucumerina YCTA2 Z1.Methods： The chemical constituents were isolated and purified by silica gel column,Sephadex LH-20,and reverse-phase C-18 column chromatography as well as crystallization.Results： Thirteen compounds were isolated from the EtOAc extract of the fungal strain YCTA2 Z1.Their chemical structures were elucidated according to the spectral evidence.They were identified as caudatin（1）,baishouwubenzophenone（2）,cynandione B（3）,asterbatanoside A（4）,p-hydroxyphenethyl-O-β-D-glycoside（5）,caffeic acid（6）,ferulic acid（7）,2,5-dihydroxyacetophenone（8）,protocatechuic acid（9）,vanillic acid（10）,stearic acid（11）,azelaic acid（12）,and succinic acid（13）.Conclusions： It is the first chemical study on endophytic fungi from Cynanchum auriculatum and all the compounds are obtained from the species,the genus,as well as the family Plectosphaerellaceae for the first time.
基金supported by the Natural Science Foundation of Jiangsu Province,China(No.BK20211067)the National Natural Science Foundation of China(No.51805145)the Qing-Lan Project of Jiangsu Province,China。
基金supported by grants from the National Key R&D Program of China(2020YFA0509500)the National Natural Science Foundation of China(U19A2036,32102512,31872335,and 31802044)+1 种基金the National Special Foundation for Transgenic Species of China(2014ZX0800605B)the Sichuan Science and Technology Program,China(2021YFYZ0009 and 2021YFYZ0030)。
文摘Follistatin(FST) is an important regulator of skeletal muscle growth and adipose deposition through its ability to bind to several members of the transforming growth factor-β(TGF-β) superfamily, and thus may be a good candidate for future animal breeding programs. However, the molecular mechanisms underlying the phenotypic changes have yet to be clarified in pig. We generated transgenic(TG) pigs that express human FST specifically in skeletal muscle tissues and characterized the phenotypic changes compared with the same tissues in wild-type pigs. The TG pigs showed increased skeletal muscle growth, decreased adipose deposition, and improved metabolism status(P<0.05). Transcriptome analysis detected important roles of the PIK3–AKT signaling pathway, calcium-mediated signaling pathway, and amino acid metabolism pathway in FST-induced skeletal muscle hypertrophy, and depot-specific oxidative metabolism changes in psoas major muscle. Furthermore, the lipid metabolism-related process was changed in adipose tissue in the TG pigs. Gene set enrichment analysis revealed that genes related to lipid synthesis, lipid catabolism, and lipid storage were down-regulated(P<0.01) in the TG pigs for subcutaneous fat, whereas genes related to lipid catabolism were significantly up-regulated(P<0.05) in the TG pigs for retroperitoneal fat compared with their expression levels in wild-type pigs. In liver, genes related to the TGF-β signaling pathway were over-represented in the TG pigs, which is consistent with the inhibitory role of FST in regulating TGF-β signaling. Together, these results provide new insights into the molecular mechanisms underlying the phenotypic changes in pig.
文摘Objective To estimate the clinical and pathological features of pancreatic solid cystic papillary tumor (SCPT) in children. Methods From 2000 to 2005, 8 cases with SCPT of the pancreas were analyzed retrospectively. All cases but one were females. Average age was 12.8 years. By case review, we discussed the clinical and pathological features of SCPT in children. Results The chief complains were abdominal pain and palpable mass. There were 3 cases in the head, 1 case in the body, and 4 cases in the tail of pancreas. The procedures employed included local resection (1 case), distal pancreatectomy (5 cases), pancreaticoduodenectomy (1 case), and biopsy (1 case). Histological examination showed solid with cystic areas and papillary protrusions in the 8 cases; as for immunohistochemical examinations, the positive rate was 100% for α-antitrypsin (AACT), 87.5% for vinmentin, and 62.5% for neuron-specific enolase (NSE). The patients were followed up for 2 months to 4 years but one was lost by follow-up and all were alive postoperatively. SCPT in 2 cases relapsed. Conclusion Occurring predominantly in young females, SCPT is usually curable by surgical resection with a favorable prognosis.
基金1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(NO.2019-088)。
文摘Chromovitrectomy is a surgical method that uses intraocular dyes during vitreoretinal surgery to stain transparent tissues in the eye such as the posterior vitreous cortex,epiretinal membrane and inner limiting membrane for easy operation.It can avoid the intraocular complications associated with incomplete vitrectomy,incomplete epiretinal membrane peeling and incomplete internal limiting membrane peeling.At present,the commonly used vital dyes in clinic are as follows:indocyanine green,infracyanine green,triamcinolone acetonide,trypan blue,brilliant blue G.Indocyanine green was first used for intraocular staining,which can show the inner limiting membrane well.Following indocyanine green,trypan blue is used to identify idiopathic epiretinal membranes and triamcinolone acetonide is used to stain the vitreous.Recently,it has been discovered that natural dyes extracted from fruits,such as lutein and anthocyanins,are also used in intraocular vitrectomy.The main purpose of this review is to explore the research status and prospects of various intraocular stains in stained vitrectomy.
基金supported by the National Science Foundation of China(Grant No.52278469)the Natural Science Foundation of Hunan Province(Grant No.2022JJ30715)。
文摘To investigate the stability of gravity anchors of suspension bridges,in-situ tests of the vertical bearing capacity of the bedrock,shear resistance of the anchor-rock interface,shear resistance of the bedrock were conducted in a suspension bridge project.Under dry-wet cycles,the deterioration law of the mechanical properties of argillaceous sandstone was identified in laboratory tests:the elastic modulus,cohesion and friction of the argillaceous sandstone deteriorated significantly at first few dry-wet cycles and then declined slowly after 10 cycles,ultimately these three mechanical parameters were reduced to about 1/3,1/3,2/3 of the initial value respectively.Moreover,numerical simulation was used to restore in-situ shear tests and a good agreement was obtained.Base on the results of in-situ and laboratory tests,the stability of the gravity anchor foundation under natural conditions and drywet cycles was calculated and its failure modes were analyzed.The results demonstrated that the dry-wet cycles caused uneven settlement of the anchor foundation,resulting in more serious stress concentration in the substrate.The dry-wet cycles remarkably reduced the stability coefficient of the anchor foundation,whose failure mode shifted from overturning failure under natural conditions to sliding failure.When there was weak interlayer in the rock layer,the anti-sliding stability of the anchor foundation was affected drastically.
文摘The technology of anti-collision is a key point in radio frequency identification （RFID） system. To avoid data collision, there are two approaches： ALOHA based algorithm and binary tree （BT） based algorithm. However, these cannot solve the collision problem completely, especially when the tag quantity is big and the tag ID is long. In this article, we present a multi-branch query tree （MBQT） protocol based on balanced incomplete block design （BIBD） code, and use 16-bit vectors derived from the BIBD as query prefix symbols of RFID reader. Compared with the conventional anti-collision algorithm, the theoretic analysis and simulation show that the proposed protocol improves the identification efficiency.
文摘Objective To investigate the diagnosis and therapy of mediastinal enterogenous cysts in children. Methods Clinical data of 17 cases with mediastinal enterogenous cysts within 19 years in our hospital were retrospectively analyzed. Results One case was intramural esophageal cyst and 16 cases were enteric cysts, two among which were complicated with abdominal enteric duplications. Most cases presented with symptoms of respiratory distress. Twelve cases were complicated with vertebral anomalies. Ultrasound helpful in confirming the cystic nature of these lesions. posterior mediastinum. of 12 cases and magnetic resonance imaging of 4 cases were Eight cases had technetium-99m pertechnetate scintigraphy of posterior mediastinum.Conclusions Most patients present with symptoms of respiratory distress, complicated with vertebral anomalies. Ultrasonography and magnetic resonance imaging may be helpful in confirming the cystic nature of these lesions. Technetium-99m pertechnetate scintigraphy is the most effective method for differentiation of the disease from other mediastinal cysts.
基金financially supported by the Project of Key Research Plan of Ningxia (Nos.2020BDE03012 and 2018BEE03008)the Science and Technology Project for Young Talents of Ningxia (No.TJGC2019042)
文摘The Mg-Al layered double hydroxide(LDH)conversion coatings were first synthesized in situ to modify the AZ91 D alloy through urea hydrolysis to adjust the pH values(9.4,10.4,11.2 and 11.4).The pH 11.2 Mg-Al LDH possessed the best compactness and good crystallinity compared to other in situ LDH coatings and obtained the lowest corrosion current density(i_(corr))of(2.884×10^(-6)±0.345×10^(-6))A·cm^(-2),which was attributed to the anion-exchange reaction of LDH and the physical barrier against corrosion owing to the twisted penetration pathway of the interlaced LDH sheets.Core-shell structured Zn-Al LDH@ZIF-8 powder modified with stearic acid(SA)was further wrapped with polyvinylidene fluoride(PVDF)to prepare a hydrophobic double-layered coating on the underlying pH 11.2 Mg-Al LDH(SLLZ).The water contact angle(CA)of the SLLZ coating reached 105.6°,and its i_(corr)decreased to(3.524×10^(-7)±0.214×10^(-7))A·cm^(-2)compared with a single pH 11.2 film.The SLLZ coating exhibited high durability and corrosion protection,even after15 days of immersion in NaCl solution.The PVDF,SA and ZIF-8 nano-shells contributed to good hydrophobicity,effectively forming a physical barrier.The Zn-Al LDH core and underlying in situ Mg-Al LDH were beneficial for synergistically promoting anion-exchange reaction between the intercalated anions of LDH and chlorides in corrosive media.This work provides a promising approach that combines core-shell LDH@ZIF-8 with LDH film on a Mg alloy surface to construct a hydrophobic film with excellent longterm anti-corrosion performance.
基金This work was supported by grants from the Ministry of Science and Technology(2017YFA0505500)the Strategic CAS Project(XDA12010000 and XDB3000000).
文摘For patients with COVID-19 caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the damages to multiple organs have been clinically observed.Since most of current investigations for virus-host interaction are based on cell level,there is an urgent demand to probe tissue-specific features associated with SARS-CoV-2 infection.Based on collected proteomic datasets from human lung,colon,kidney,liver,and heart,we constructed a virus-receptor network,a virus-interaction network,and a virus-perturbation network.In the tissue-specific networks associated with virus-host crosstalk,both common and different key hubs are revealed in diverse tissues.Ubiquitous hubs in multiple tissues such as BRD4 and RIPK1 would be promising drug targets to rescue multi-organ injury and deal with inflammation.Certain tissue-unique hubs such as REEP5 might mediate specific olfactory dysfunction.The present analysis implies that SARS-CoV-2 could affect multi-targets in diverse host tissues,and the treatment of COVID-19 would be a complex task.
基金supported by the Key Project of NSFC-Henan Province Joint Fund,China(U1704233)the Innovation Research Team of Ministry of Education,China(IRT-16R23)+1 种基金the Program for Innovative Research Team(in Science and Technology)in University of Henan Province,China(21IRTSTHN022)the Key Scientific Research Project of Higher Education of Henan Province(21A230011)。
文摘Indigenous chicken products are increasingly favored by consumers due to their unique meat and egg quality.However,the relatively poor egg-laying performance largely impacts the economic benefits and hinders sustainable development of the local chicken industry.Thus,excavating key genes and effective molecular markers associated with egg-laying performance is necessary to improve egg production via genetic selection in indigenous breeds.In the present study,comparative hypothalamic transcriptome between pre-laying(15 weeks old)and peak-laying(30 weeks old)Lushi blueshelled-egg(LBS)chicken was performed.A total of 518 differentially expressed genes(DEGs)were identified.Among the DEGs,64 genes were enriched in 10 Gene Ontology(GO)terms associated with reproductive regulation via GO analysis and considered as potential candidate genes regulating egg-laying performance.Of the 64 genes,16 showed high connectivity(degree≥12)by protein–protein interaction(PPI)network analysis and were considered as potential core candidate genes(PCCGs).To further look for key candidate genes from the PCCGs,firstly,the expression patterns of the 16 genes were examined in the hypothalamus of two indigenous breeds(LBS and Gushi(GS)chickens)between the pre-laying and peak-laying stages using quantitative real-time PCR(qRT-PCR).Eleven out of the 16 genes showed significantly differential expression(P<0.05)with the same changing trends in the two breeds.Then,correlations between the expression levels of the above 11 genes and egg numbers and reproductive hormone concentrations in serum were investigated in high-yielding and low-yielding GS chickens.Of the 11 genes,eight showed significant correlations(P<0.05)between their expression levels and egg numbers,and between expression levels and reproductive hormone concentration in serum.Furthermore,an association study on single nucleotide polymorphisms(SNPs)identified in these eight genes and egg production traits was carried out in 640 GS hens,and a significant association(P<0.05)between the
文摘RNA can interact with RNA-binding proteins(RBPs),mRNA,or other non-coding RNAs(ncRNAs)to form complex regulatory networks.High-throughput CLIP-seq,degradome-seq,and RNA-RNA interactome sequencing methods represent powerful approaches to identify biologically relevant ncRNA-target and protein-ncRNA interactions.However,assigning ncRNAs to their regulatory target genes or interacting RNA-binding proteins(RBPs)remains technically challenging.Chemical modifications to mRNA also play important roles in regulating gene expression.Investigation of the functional roles of these modifications relies highly on the detection methods used.RNA structure is also critical at nearly every step of the RNA life cycle.In this review,we summarize recent advances and limitations in CLIP technologies and discuss the computational challenges of and bioinformatics tools used for decoding the functions and regulatory networks of ncRNAs.We also summarize methods used to detect RNA modifications and to probe RNA structure.
文摘To improve the spectrum sensing performance in cognitive radios, a scheme of cooperative blind spectrum sensing based on autocorrelation matrix is proposed. The test statistic is extracted from the autocorrelation matrix of the received signal samples and a bi-threshold hybrid decision scheme is designed for local spectrum sensing. The cognitive radio base station makes a credibility fusion based on the local soft decisions and then takes global fusion combining with the local hard decisions. The proposed method is blind in the sense since it requires no apriori knowledge of the signal and the noise power. Theoretical analysis and computer simulation results show that the proposed method can enhance the spectrum sensing capability.
基金The Open Project of the State Key Laboratory of Robotics and System (HIT)the State Key Laboratory of Cognitive Neuroscience and Learning+3 种基金Natural Science Fund for Colleges and Universities in Jiangsu Provincegrant number:105TB51003Natural Science Fund in Changzhougrant number:CJ20110023
文摘Accurate classification of EEG left and right hand motor imagery is an important issue in brain-computer interface. Firstly, discrete wavelet transform method was used to decompose the average power of C3 electrode and C4 electrode in left-right hands imagery movement during some periods of time. The reconstructed signal of approximation coefficient A6 on the 6al level was selected to build up a feature signal. Secondly, the performances by Fisher Linear Discriminant Analysis with two different threshold calculation ways and Support Vector Machine methods were compared. The final classification results showed that false classification rate by Support Vector Machine was lower and gained an ideal classification results.
