This paper provides a bird's-eye view both in preclinical and clinical aspects of E-cadherin germline gene (CDH1) in gastric cancer patients and their families. E-cadherin, a product of CDH1 gene, belonging to the...This paper provides a bird's-eye view both in preclinical and clinical aspects of E-cadherin germline gene (CDH1) in gastric cancer patients and their families. E-cadherin, a product of CDH1 gene, belonging to the functionally related trans-membrane glycoprotein family, is responsible for the Ca^2+-dependent cell-cell adhesion mechanism and contributes to dissociation followed by acquisition of cell motility, which usually occurs in the first step of cancer irwasion and metastasis. CDH1 gene germline mutation is common in many types of carcinoma, and occurs very frequent in hereditary gastric carcinoma (HGC) patients and their families. Recently, more and more researches support that E-cadherin plays an important role in the differentiation, growth and invasion of HGC. So it is of great value to clarify its mechanisms both for understanding HGC pathogenesis and for clinical therapy, especially in China, where there are a high risk population of gastric cancer and a high HGC incidence rate. In this paper, recent researches on CDH1 gene mutation, especially its role in tumor genesis and progress of HGC, are reviewed, and advances in evaluation of its mutation status for HGC diagnosis, therapy and prognosis, are also discussed briefly.展开更多
In the field of satellite imagery, remote sensing image captioning(RSIC) is a hot topic with the challenge of overfitting and difficulty of image and text alignment. To address these issues, this paper proposes a visi...In the field of satellite imagery, remote sensing image captioning(RSIC) is a hot topic with the challenge of overfitting and difficulty of image and text alignment. To address these issues, this paper proposes a vision-language aligning paradigm for RSIC to jointly represent vision and language. First, a new RSIC dataset DIOR-Captions is built for augmenting object detection in optical remote(DIOR) sensing images dataset with manually annotated Chinese and English contents. Second, a Vision-Language aligning model with Cross-modal Attention(VLCA) is presented to generate accurate and abundant bilingual descriptions for remote sensing images. Third, a crossmodal learning network is introduced to address the problem of visual-lingual alignment. Notably, VLCA is also applied to end-toend Chinese captions generation by using the pre-training language model of Chinese. The experiments are carried out with various baselines to validate VLCA on the proposed dataset. The results demonstrate that the proposed algorithm is more descriptive and informative than existing algorithms in producing captions.展开更多
Surface modification for micro-nanoparticles at the atomic and close-to-atomic scales is of great importance to enhance their performance in various applications,including high-volume battery,persistent luminescence,e...Surface modification for micro-nanoparticles at the atomic and close-to-atomic scales is of great importance to enhance their performance in various applications,including high-volume battery,persistent luminescence,etc.Fluidized bed atomic layer deposition(FB-ALD)is a promising atomic-scale manufacturing technology that offers ultrathin films on large amounts of particulate materials.Nevertheless,nanoparticles tend to agglomerate due to the strong cohesive forces,which is much unfavorable to the film conformality and also hinders their real applications.In this paper,the particle fluidization process in an ultrasonic vibration-assisted FB-ALD reactor is numerically investigated from micro-scale to macro-scale through the multiscale computational fluid dynamics and discrete element method(CFD-DEM)modeling with experimental verification.Various vibration amplitudes and frequencies are investigated in terms of their effects on the fluid dynamics,distribution of particle velocity and solid volume fraction,as well as the size of agglomerates.Results show that the fluid turbulent kinetic energy,which is the key power source for the particles to obtain the kinetic energy for overcoming the interparticle agglomeration forces,can be strengthened obviously by the ultrasonic vibration.Besides,the application of ultrasonic vibration is found to reduce the mean agglomerate size in the FB.This is bound to facilitate the heat transfer and precursor diffusion in the entire FB-ALD reactor and the agglomerates,which can largely shorten the coating time and improve the film conformality as well as precursor utilization.The simulation results also agree well with our battery experimental results,verifying the validity of the multiscale CFD-DEM model.This work has provided momentous guidance to the mass manufacturing of atomic-scale particle coating from lab-scale to industrial applications.展开更多
The topological connectivity information derived from the brain functional network can bring new insights for diagnosing and analyzing dementia disorders.The brain functional network is suitable to bridge the correlat...The topological connectivity information derived from the brain functional network can bring new insights for diagnosing and analyzing dementia disorders.The brain functional network is suitable to bridge the correlation between abnormal connectivities and dementia disorders.However,it is challenging to access considerable amounts of brain functional network data,which hinders the widespread application of data-driven models in dementia diagnosis.In this study,a novel distribution-regularized adversarial graph auto-Encoder(DAGAE)with transformer is proposed to generate new fake brain functional networks to augment the brain functional network dataset,improving the dementia diagnosis accuracy of data-driven models.Specifically,the label distribution is estimated to regularize the latent space learned by the graph encoder,which canmake the learning process stable and the learned representation robust.Also,the transformer generator is devised to map the node representations into node-to-node connections by exploring the long-term dependence of highly-correlated distant brain regions.The typical topological properties and discriminative features can be preserved entirely.Furthermore,the generated brain functional networks improve the prediction performance using different classifiers,which can be applied to analyze other cognitive diseases.Attempts on the Alzheimer’s Disease Neuroimaging Initiative(ADNI)dataset demonstrate that the proposed model can generate good brain functional networks.The classification results show adding generated data can achieve the best accuracy value of 85.33%,sensitivity value of 84.00%,specificity value of 86.67%.The proposed model also achieves superior performance compared with other related augmentedmodels.Overall,the proposedmodel effectively improves cognitive disease diagnosis by generating diverse brain functional networks.展开更多
Lysine succinylation is a novel,naturally occurring posttranslational modification(PTM)in living organisms.Global lysine succinylation identification has been performed at the proteomic level in various species;howeve...Lysine succinylation is a novel,naturally occurring posttranslational modification(PTM)in living organisms.Global lysine succinylation identification has been performed at the proteomic level in various species;however,the study of lysine succinylation in plant species is relatively limited.Patchouli plant(P.cablin(Blanco)Benth.,Lamiaceae)is a globally important industrial plant and medicinal herb.In the present study,lysine succinylome analysis was carried out in patchouli plants to determine the potential regulatory role of lysine succinylation in patchouli growth,development,and physiology.The global succinylation sites and proteins in patchouli plants were screened with an immunoprecipitation affinity enrichment technique and advanced mass spectrometry-based proteomics.Several bioinformatic analyses,such as function classification and enrichment,subcellular location predication,metabolic pathway enrichment and protein−protein interaction networking,were conducted to characterize the functions of the identified sites and proteins.In total,1097 succinylation sites in 493 proteins were detected in patchouli plants,among which 466 succinylation sites in 241 proteins were repeatedly identified within three independent experiments.The functional characterization of these proteins indicated that the tricarboxylic acid(TCA)cycle,oxidative phosphorylation,photosynthesis processes,and amino acid biosynthesis may be regulated by lysine succinylation.In addition,these succinylated proteins showed a wide subcellular location distribution,although the chloroplast and cytoplasm were the top two preferred cellular components.Our study suggested the important role of lysine succinylation in patchouli plant physiology and biology and could serve as a useful reference for succinylation studies in other medicinal plants.展开更多
Ribosome biogenesis is essential for the cell growth and division. Disruptions in ribosome biogenesis result in developmental defects and a group of diseases, known as ribosomopathies. Here, we report a mutation in ze...Ribosome biogenesis is essential for the cell growth and division. Disruptions in ribosome biogenesis result in developmental defects and a group of diseases, known as ribosomopathies. Here, we report a mutation in zebrafish urb1, which encodes an essential ribosome biogenesis protein. The urb1 cq31 mutant exhibits hypoplastic digestive organs, which is caused by impaired cell proliferation with the differentiation of digestive organ progenitors unaffected. Knockdown of mtor or raptor leads to similar hypoplastic phenotypes and reduced expression of urb1 in the digestive organs. Overexpression of Urb1 results in overgrowth of digestive organs, and can efficiently rescue the hypoplastic liver and pancreas in the mtor and raptor morphants. Reduced syntheses of free ribosomal subunits and impaired assembly of polysomes are observed in the urb1 mutant as well as in the mtor and raptor morphants, which can be rescued by the Urb1 overexpression. These data demonstrate that Urb1 plays an important role in governing ribosome biogenesis and protein synthesis downstream of mammalian/mechanistic target of rapamycin complex 1（mTORC1）, thus regulating the development of digestive organs. Our study indicates the requirement of hyperactive protein synthesis for the digestive organ development.展开更多
On planet earth, the only place so far known that is suited to human life, human beings are the life form with the greatest vitality and ability. Our attitudes and concepts in relation to the world should not stop at ...On planet earth, the only place so far known that is suited to human life, human beings are the life form with the greatest vitality and ability. Our attitudes and concepts in relation to the world should not stop at understanding and changing it; rather, they should be sublated into a new worldview through which we can scientifically understand and rationally improve the world. Only on the basis of this new worldview can we find a new focus and a new set of values, a new way of living and acting. ＂Beautiful China,＂ as a literary metaphor for the goal of constructing ecological civilization, vividly and fully expresses a completely new vision for socialist modernization with Chinese characteristics. The building of ecological civilization is a social scheme that needs to be based on clear, comprehensive, long-term and scientific ecological values, guided by ideas and regulated by institutions. It calls for the conscious action of all members of society.展开更多
AIM: To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC).METHODS: The full length AFP-cDNA of prokaryotic vector was di...AIM: To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC).METHODS: The full length AFP-cDNA of prokaryotic vector was digested, and subcloned to the multi-clony sites of the eukaryotic vector. The constructed vector was confirmed by enzymes digestion and electrophoresis, and the product expressed was detected by electrochemiluminescence and immunofluorescence methods.RESULTS: The full length AFP-cDNA successfully cloned to the eukaryotic vector through electrophoresis, 0.9723 IU/ml AFP antigen was detected in the supernatant of AFPCHO by electrochemiluminescence method. Compared with the control groups, the differences were significant (P＜0.05).AFP antigen molecule was observed in the plasma of AFPCHO by immunofluorescence staining.CONCLUSION: The recombined human AFP eukaryotic expression vector can express in CHO cell line. It provides experimental data for gene therapy and target therapy of hepatocellular carcinoma.展开更多
The multiple images of modernity are used here to put forward and corroborate the possibility of the genesis of multiple modern civilizations and their very modernity while confirming the prior existence of modern Wes...The multiple images of modernity are used here to put forward and corroborate the possibility of the genesis of multiple modern civilizations and their very modernity while confirming the prior existence of modern Western civilization. In examining the complexity, inner tensions and contemporary predicaments appearing in the formation and evolution of earlier Western modernity, this paper reveals the basic mechanisms of modernity and the various possible approaches to it, that is, the fact that there are more possibilities for modernity other than the Western one. Economic globalization is the first step and the only pathway towards the modernity goals of human society, and any deglobalization or antiglobalization will hinder or check the progress of human society towards the ideal goal of modernity. History and the real-world economy demonstrate that “building a community with a shared future for mankind” is so far a more suitable and more explanatory guiding concept for seeking and approaching the human ideal of modernity;it surpasses existing concepts such as “ecumenicalism,” “cosmopolitanism,” and “globalism,” and is the latest understanding of the modernity of human society in contemporary China.展开更多
Data hiding plays an important role in privacy protection and authentication,but most data hiding methods fail to achieve satisfactory performance in resisting scaling attacks and additive attacks.To this end,this pap...Data hiding plays an important role in privacy protection and authentication,but most data hiding methods fail to achieve satisfactory performance in resisting scaling attacks and additive attacks.To this end,this paper proposes a new quantization index modulation(QIM)variant based on division domains(D-QIM).It can not only resist the above two attacks well,but also adjust the performance trade-offs by controlling the parameters.Simulation results confirm the performance gain of D-QIM in terms of the bit error rate(BER).展开更多
Attractive interaction between electrons(or neutral fermions)is responsible for superconductivity(or superfluidity).In condensed matter systems,attractive interaction is usually induced by the boson-exchange mechanism...Attractive interaction between electrons(or neutral fermions)is responsible for superconductivity(or superfluidity).In condensed matter systems,attractive interaction is usually induced by the boson-exchange mechanism[1].Indeed,in the celebrated Bardeen-Cooper-Schreiffer(BCS)theory,electrons develop attractive interaction by exchanging phonons[2].Subsequent studies show that other collective excitations such as charge density waves[3]and spin fluctuations[4]can also induce attractive interaction.The bosonexchange mechanism,together with the concept of Cooperpair,is considered to be the cornerstone of the modern theory of superconductivity.展开更多
Abstract Experimental examinations and analyses have been made of the sediments from drill holes in the Zhuhai area, Guangdong Province. The studies show that there occurred four transgressions in this area over the h...Abstract Experimental examinations and analyses have been made of the sediments from drill holes in the Zhuhai area, Guangdong Province. The studies show that there occurred four transgressions in this area over the historical times. The first transgression occurred at 7500 a B.P., with the maxium sea level up to about 1 m above the present level. The second transgression occurred at 5750 a B.P., with the sea level possibly 4 m higher than the present. The third and fourth transgressions took place at 4650 a B.P. and 2600 a B.P. respectively, which lasted for a long time with multiple fluctuations. The fifth transgression is in process. It could be predicted that the sea level at Zhuhai would rise by 15–20 cm in the coming 50 years.展开更多
AIM: To determine the pathological characteristics of gastric leiomyoblastoma.METHODS: All tissues were obtained during surgery or gastroscopy. Tissue specimens for examination by light microscope were 1 cmxl cmxl cm ...AIM: To determine the pathological characteristics of gastric leiomyoblastoma.METHODS: All tissues were obtained during surgery or gastroscopy. Tissue specimens for examination by light microscope were 1 cmxl cmxl cm in size, fixed in 40 g/L neutral buffered formaldehyde, embedded in paraffin, and stained with hematoxylin and eosin. The fresh tissues obtained for electron microscopy were 1 mmxl mmxl mm in size, and fixed in phosphate buffered 30 g/L glutaraldehyde, postfixed in 10 g/L osmium tetroxide and dehydrated in graded alcohol, embebbed in Epon 812. UItrathin sections of 50 nm were stained with uranyl acetate and lead citrate and examined under a JEM-2000 EX transmission electron microscope.RESULTS: The most important histopathological feature of leiomyoblastoma was the predominance of large, rounded or polygonal cells with characteristic perinuclear clear zone in cytoplasms. The tumor cells arranged in patch, cell junction or junctional complex could be found occasionally between cells under electron microscope. Most of the neoplastic cytoplasms were filled with myofilaments, dense bodies, and dense patches. Rough endoplasmic reticulum dilatated as lakes, and large quantities of protein secretions of intermediate electron density were found in the dilated cisternae. Intracisternal segregation could also be found. The nuclei were round or oval, and anomalous nuclei were found in part of cells.CONCLUSION: The diagnosis of gastric leiomyoblastoma can be confirmed by electron microscopy. The clear appearance of tumor cells is due to the dilation of rough endoplasmic reticulum, not fat droplets, glycogens or mucus in cytoplasm.展开更多
In this paper,we consider weak horseshoe with bounded-gap-hitting times.For a flow(M,Ф),it is shown that if the time one map(M,Ф_(1))has weak horseshoe with boundedgap-hitting times,so is(M,Ф_(τ))for all τ≠0.In ...In this paper,we consider weak horseshoe with bounded-gap-hitting times.For a flow(M,Ф),it is shown that if the time one map(M,Ф_(1))has weak horseshoe with boundedgap-hitting times,so is(M,Ф_(τ))for all τ≠0.In addition,we prove that for an affine homeomorphism of a compact metric abelian group,positive topological entropy is equivalent to weak horseshoe with bounded-gap-hitting times.展开更多
With the growing concern on data rates and resource utilization, Device-to-Device (D2D) communication has been raised in 3GPP Long-Term Evolution (LTE) networks. In order to limit severe interference, previous studies...With the growing concern on data rates and resource utilization, Device-to-Device (D2D) communication has been raised in 3GPP Long-Term Evolution (LTE) networks. In order to limit severe interference, previous studies mainly focus on intra-cell interference that between cellular links and local D2D links. In this paper, we consider both intra-cell interference and inter-cell interference between D2D and cellular links. We propose a new resource reuse algorithm that D2D users reuse the minimum interference uplink (UL) Semi-Persistent Scheduling (SPS) resources to reach the highest throughput. The simulation results show that this scheme reduces interference as well as improves throughput.展开更多
基金Supported by the Teaching and Research Award Program for Outstanding Young Teachers,Ministry of Education,P R China,No.TRAPOYT99-016
文摘This paper provides a bird's-eye view both in preclinical and clinical aspects of E-cadherin germline gene (CDH1) in gastric cancer patients and their families. E-cadherin, a product of CDH1 gene, belonging to the functionally related trans-membrane glycoprotein family, is responsible for the Ca^2+-dependent cell-cell adhesion mechanism and contributes to dissociation followed by acquisition of cell motility, which usually occurs in the first step of cancer irwasion and metastasis. CDH1 gene germline mutation is common in many types of carcinoma, and occurs very frequent in hereditary gastric carcinoma (HGC) patients and their families. Recently, more and more researches support that E-cadherin plays an important role in the differentiation, growth and invasion of HGC. So it is of great value to clarify its mechanisms both for understanding HGC pathogenesis and for clinical therapy, especially in China, where there are a high risk population of gastric cancer and a high HGC incidence rate. In this paper, recent researches on CDH1 gene mutation, especially its role in tumor genesis and progress of HGC, are reviewed, and advances in evaluation of its mutation status for HGC diagnosis, therapy and prognosis, are also discussed briefly.
基金supported by the National Natural Science Foundation of China (61702528,61806212)。
文摘In the field of satellite imagery, remote sensing image captioning(RSIC) is a hot topic with the challenge of overfitting and difficulty of image and text alignment. To address these issues, this paper proposes a vision-language aligning paradigm for RSIC to jointly represent vision and language. First, a new RSIC dataset DIOR-Captions is built for augmenting object detection in optical remote(DIOR) sensing images dataset with manually annotated Chinese and English contents. Second, a Vision-Language aligning model with Cross-modal Attention(VLCA) is presented to generate accurate and abundant bilingual descriptions for remote sensing images. Third, a crossmodal learning network is introduced to address the problem of visual-lingual alignment. Notably, VLCA is also applied to end-toend Chinese captions generation by using the pre-training language model of Chinese. The experiments are carried out with various baselines to validate VLCA on the proposed dataset. The results demonstrate that the proposed algorithm is more descriptive and informative than existing algorithms in producing captions.
基金supported by the National Natural Science Foundation of China(51835005 and 51911540476)National Key Research and Development Program of China(2020YFB2010401)+3 种基金Hubei Province Natural Science Foundation for innovative research groups(2020CFA030)Independent Innovation Research Fund of HUST(2019kfyXMBZ025)Tencent Foundationthe Engineering and Physical Sciences Research Council project(EP/T019085/1).
文摘Surface modification for micro-nanoparticles at the atomic and close-to-atomic scales is of great importance to enhance their performance in various applications,including high-volume battery,persistent luminescence,etc.Fluidized bed atomic layer deposition(FB-ALD)is a promising atomic-scale manufacturing technology that offers ultrathin films on large amounts of particulate materials.Nevertheless,nanoparticles tend to agglomerate due to the strong cohesive forces,which is much unfavorable to the film conformality and also hinders their real applications.In this paper,the particle fluidization process in an ultrasonic vibration-assisted FB-ALD reactor is numerically investigated from micro-scale to macro-scale through the multiscale computational fluid dynamics and discrete element method(CFD-DEM)modeling with experimental verification.Various vibration amplitudes and frequencies are investigated in terms of their effects on the fluid dynamics,distribution of particle velocity and solid volume fraction,as well as the size of agglomerates.Results show that the fluid turbulent kinetic energy,which is the key power source for the particles to obtain the kinetic energy for overcoming the interparticle agglomeration forces,can be strengthened obviously by the ultrasonic vibration.Besides,the application of ultrasonic vibration is found to reduce the mean agglomerate size in the FB.This is bound to facilitate the heat transfer and precursor diffusion in the entire FB-ALD reactor and the agglomerates,which can largely shorten the coating time and improve the film conformality as well as precursor utilization.The simulation results also agree well with our battery experimental results,verifying the validity of the multiscale CFD-DEM model.This work has provided momentous guidance to the mass manufacturing of atomic-scale particle coating from lab-scale to industrial applications.
基金This paper is partially supported by the British Heart Foundation Accelerator Award,UK(AA\18\3\34220)Royal Society International Exchanges Cost Share Award,UK(RP202G0230)+9 种基金Hope Foundation for Cancer Research,UK(RM60G0680)Medical Research Council Confidence in Concept Award,UK(MC_PC_17171)Sino-UK Industrial Fund,UK(RP202G0289)Global Challenges Research Fund(GCRF),UK(P202PF11)LIAS Pioneering Partnerships Award,UK(P202ED10)Data Science Enhancement Fund,UK(P202RE237)Fight for Sight,UK(24NN201)Sino-UK Education Fund,UK(OP202006)Biotechnology and Biological Sciences Research Council,UK(RM32G0178B8)LIAS Seed Corn,UK(P202RE969).
文摘The topological connectivity information derived from the brain functional network can bring new insights for diagnosing and analyzing dementia disorders.The brain functional network is suitable to bridge the correlation between abnormal connectivities and dementia disorders.However,it is challenging to access considerable amounts of brain functional network data,which hinders the widespread application of data-driven models in dementia diagnosis.In this study,a novel distribution-regularized adversarial graph auto-Encoder(DAGAE)with transformer is proposed to generate new fake brain functional networks to augment the brain functional network dataset,improving the dementia diagnosis accuracy of data-driven models.Specifically,the label distribution is estimated to regularize the latent space learned by the graph encoder,which canmake the learning process stable and the learned representation robust.Also,the transformer generator is devised to map the node representations into node-to-node connections by exploring the long-term dependence of highly-correlated distant brain regions.The typical topological properties and discriminative features can be preserved entirely.Furthermore,the generated brain functional networks improve the prediction performance using different classifiers,which can be applied to analyze other cognitive diseases.Attempts on the Alzheimer’s Disease Neuroimaging Initiative(ADNI)dataset demonstrate that the proposed model can generate good brain functional networks.The classification results show adding generated data can achieve the best accuracy value of 85.33%,sensitivity value of 84.00%,specificity value of 86.67%.The proposed model also achieves superior performance compared with other related augmentedmodels.Overall,the proposedmodel effectively improves cognitive disease diagnosis by generating diverse brain functional networks.
基金support for this research was provided in part by a grant from Project 81803657 supported by the National Natural Science Foundation of Chinathe Guangdong Education department Key Promotion Platform Construction Project,Lingnan Key Laboratory of Chinese Medicine Resources Ministry of Education(2014KTSPT016)+1 种基金special funds for the Construction of Traditional Chinese Medicine in Guangdong Province(No.20181075)the earmarked fund for Guangdong Education Department Innovation Strong School Project(No.E1-KFD015181K28/2017KQNCX039).
文摘Lysine succinylation is a novel,naturally occurring posttranslational modification(PTM)in living organisms.Global lysine succinylation identification has been performed at the proteomic level in various species;however,the study of lysine succinylation in plant species is relatively limited.Patchouli plant(P.cablin(Blanco)Benth.,Lamiaceae)is a globally important industrial plant and medicinal herb.In the present study,lysine succinylome analysis was carried out in patchouli plants to determine the potential regulatory role of lysine succinylation in patchouli growth,development,and physiology.The global succinylation sites and proteins in patchouli plants were screened with an immunoprecipitation affinity enrichment technique and advanced mass spectrometry-based proteomics.Several bioinformatic analyses,such as function classification and enrichment,subcellular location predication,metabolic pathway enrichment and protein−protein interaction networking,were conducted to characterize the functions of the identified sites and proteins.In total,1097 succinylation sites in 493 proteins were detected in patchouli plants,among which 466 succinylation sites in 241 proteins were repeatedly identified within three independent experiments.The functional characterization of these proteins indicated that the tricarboxylic acid(TCA)cycle,oxidative phosphorylation,photosynthesis processes,and amino acid biosynthesis may be regulated by lysine succinylation.In addition,these succinylated proteins showed a wide subcellular location distribution,although the chloroplast and cytoplasm were the top two preferred cellular components.Our study suggested the important role of lysine succinylation in patchouli plant physiology and biology and could serve as a useful reference for succinylation studies in other medicinal plants.
基金supported by the National Key Basic Research Program of China(2015CB942800)the National Natural Science Foundation of China(Nos.31330051 and 31730060)+2 种基金the 111 Program(B14037)the Natural Science Foundation Project of Chongqing(cstc2014jcyj A10088)the Fundamental Research Funds for the Central Universities(XDJK2015B011)
文摘Ribosome biogenesis is essential for the cell growth and division. Disruptions in ribosome biogenesis result in developmental defects and a group of diseases, known as ribosomopathies. Here, we report a mutation in zebrafish urb1, which encodes an essential ribosome biogenesis protein. The urb1 cq31 mutant exhibits hypoplastic digestive organs, which is caused by impaired cell proliferation with the differentiation of digestive organ progenitors unaffected. Knockdown of mtor or raptor leads to similar hypoplastic phenotypes and reduced expression of urb1 in the digestive organs. Overexpression of Urb1 results in overgrowth of digestive organs, and can efficiently rescue the hypoplastic liver and pancreas in the mtor and raptor morphants. Reduced syntheses of free ribosomal subunits and impaired assembly of polysomes are observed in the urb1 mutant as well as in the mtor and raptor morphants, which can be rescued by the Urb1 overexpression. These data demonstrate that Urb1 plays an important role in governing ribosome biogenesis and protein synthesis downstream of mammalian/mechanistic target of rapamycin complex 1（mTORC1）, thus regulating the development of digestive organs. Our study indicates the requirement of hyperactive protein synthesis for the digestive organ development.
文摘On planet earth, the only place so far known that is suited to human life, human beings are the life form with the greatest vitality and ability. Our attitudes and concepts in relation to the world should not stop at understanding and changing it; rather, they should be sublated into a new worldview through which we can scientifically understand and rationally improve the world. Only on the basis of this new worldview can we find a new focus and a new set of values, a new way of living and acting. ＂Beautiful China,＂ as a literary metaphor for the goal of constructing ecological civilization, vividly and fully expresses a completely new vision for socialist modernization with Chinese characteristics. The building of ecological civilization is a social scheme that needs to be based on clear, comprehensive, long-term and scientific ecological values, guided by ideas and regulated by institutions. It calls for the conscious action of all members of society.
基金Teaching and Research Award Program for Outstanding Young Teacher in Higher Education Institutes,No.TRAPOYT99-016the Fund for Distinguished Young Scholars of Chinese PLA,No.01J016
文摘AIM: To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC).METHODS: The full length AFP-cDNA of prokaryotic vector was digested, and subcloned to the multi-clony sites of the eukaryotic vector. The constructed vector was confirmed by enzymes digestion and electrophoresis, and the product expressed was detected by electrochemiluminescence and immunofluorescence methods.RESULTS: The full length AFP-cDNA successfully cloned to the eukaryotic vector through electrophoresis, 0.9723 IU/ml AFP antigen was detected in the supernatant of AFPCHO by electrochemiluminescence method. Compared with the control groups, the differences were significant (P＜0.05).AFP antigen molecule was observed in the plasma of AFPCHO by immunofluorescence staining.CONCLUSION: The recombined human AFP eukaryotic expression vector can express in CHO cell line. It provides experimental data for gene therapy and target therapy of hepatocellular carcinoma.
文摘The multiple images of modernity are used here to put forward and corroborate the possibility of the genesis of multiple modern civilizations and their very modernity while confirming the prior existence of modern Western civilization. In examining the complexity, inner tensions and contemporary predicaments appearing in the formation and evolution of earlier Western modernity, this paper reveals the basic mechanisms of modernity and the various possible approaches to it, that is, the fact that there are more possibilities for modernity other than the Western one. Economic globalization is the first step and the only pathway towards the modernity goals of human society, and any deglobalization or antiglobalization will hinder or check the progress of human society towards the ideal goal of modernity. History and the real-world economy demonstrate that “building a community with a shared future for mankind” is so far a more suitable and more explanatory guiding concept for seeking and approaching the human ideal of modernity;it surpasses existing concepts such as “ecumenicalism,” “cosmopolitanism,” and “globalism,” and is the latest understanding of the modernity of human society in contemporary China.
基金supported in part by the National Natural Science Foundation of China (61902149,61932010 and 62032009)the Natural Science Foundation of Guangdong Province (2020A1515010393).
文摘Data hiding plays an important role in privacy protection and authentication,but most data hiding methods fail to achieve satisfactory performance in resisting scaling attacks and additive attacks.To this end,this paper proposes a new quantization index modulation(QIM)variant based on division domains(D-QIM).It can not only resist the above two attacks well,but also adjust the performance trade-offs by controlling the parameters.Simulation results confirm the performance gain of D-QIM in terms of the bit error rate(BER).
基金supported by the Program of “One Hundred Talented People” of Chinese Academy of Sciencesthe National Natural Science Foundation of China(Grant No.10604063)supported by the Welch Foundation(Grant No.F-1255)
文摘Attractive interaction between electrons(or neutral fermions)is responsible for superconductivity(or superfluidity).In condensed matter systems,attractive interaction is usually induced by the boson-exchange mechanism[1].Indeed,in the celebrated Bardeen-Cooper-Schreiffer(BCS)theory,electrons develop attractive interaction by exchanging phonons[2].Subsequent studies show that other collective excitations such as charge density waves[3]and spin fluctuations[4]can also induce attractive interaction.The bosonexchange mechanism,together with the concept of Cooperpair,is considered to be the cornerstone of the modern theory of superconductivity.
文摘Abstract Experimental examinations and analyses have been made of the sediments from drill holes in the Zhuhai area, Guangdong Province. The studies show that there occurred four transgressions in this area over the historical times. The first transgression occurred at 7500 a B.P., with the maxium sea level up to about 1 m above the present level. The second transgression occurred at 5750 a B.P., with the sea level possibly 4 m higher than the present. The third and fourth transgressions took place at 4650 a B.P. and 2600 a B.P. respectively, which lasted for a long time with multiple fluctuations. The fifth transgression is in process. It could be predicted that the sea level at Zhuhai would rise by 15–20 cm in the coming 50 years.
基金Supported by the Foundation of 117 Hospital,No.99008
文摘AIM: To determine the pathological characteristics of gastric leiomyoblastoma.METHODS: All tissues were obtained during surgery or gastroscopy. Tissue specimens for examination by light microscope were 1 cmxl cmxl cm in size, fixed in 40 g/L neutral buffered formaldehyde, embedded in paraffin, and stained with hematoxylin and eosin. The fresh tissues obtained for electron microscopy were 1 mmxl mmxl mm in size, and fixed in phosphate buffered 30 g/L glutaraldehyde, postfixed in 10 g/L osmium tetroxide and dehydrated in graded alcohol, embebbed in Epon 812. UItrathin sections of 50 nm were stained with uranyl acetate and lead citrate and examined under a JEM-2000 EX transmission electron microscope.RESULTS: The most important histopathological feature of leiomyoblastoma was the predominance of large, rounded or polygonal cells with characteristic perinuclear clear zone in cytoplasms. The tumor cells arranged in patch, cell junction or junctional complex could be found occasionally between cells under electron microscope. Most of the neoplastic cytoplasms were filled with myofilaments, dense bodies, and dense patches. Rough endoplasmic reticulum dilatated as lakes, and large quantities of protein secretions of intermediate electron density were found in the dilated cisternae. Intracisternal segregation could also be found. The nuclei were round or oval, and anomalous nuclei were found in part of cells.CONCLUSION: The diagnosis of gastric leiomyoblastoma can be confirmed by electron microscopy. The clear appearance of tumor cells is due to the dilation of rough endoplasmic reticulum, not fat droplets, glycogens or mucus in cytoplasm.
基金Leiye Xu is partially supported by NNSF of China(11801538,11871188)the USTC Research Funds of the Double First-Class Initiative.Junren Zheng is partially supported by NNSF of China(11971455).
文摘In this paper,we consider weak horseshoe with bounded-gap-hitting times.For a flow(M,Ф),it is shown that if the time one map(M,Ф_(1))has weak horseshoe with boundedgap-hitting times,so is(M,Ф_(τ))for all τ≠0.In addition,we prove that for an affine homeomorphism of a compact metric abelian group,positive topological entropy is equivalent to weak horseshoe with bounded-gap-hitting times.
文摘With the growing concern on data rates and resource utilization, Device-to-Device (D2D) communication has been raised in 3GPP Long-Term Evolution (LTE) networks. In order to limit severe interference, previous studies mainly focus on intra-cell interference that between cellular links and local D2D links. In this paper, we consider both intra-cell interference and inter-cell interference between D2D and cellular links. We propose a new resource reuse algorithm that D2D users reuse the minimum interference uplink (UL) Semi-Persistent Scheduling (SPS) resources to reach the highest throughput. The simulation results show that this scheme reduces interference as well as improves throughput.
