As contamination is one of important factors to Panax notoginseng quality and safety. Saponin is one of important compounds with the medicinal values of P. notoginseng. The impact of soil As on production of saponin o...As contamination is one of important factors to Panax notoginseng quality and safety. Saponin is one of important compounds with the medicinal values of P. notoginseng. The impact of soil As on production of saponin of P. notoginseng knew very little. This study was performed to determine content and heterogeneity of saponins from P. notoginseng and its mechanisms upon treatments with different concentration levels of As in soil. Plants of P. notoginseng were treated with arsenic [As (V)] at 0, 20, 80, 140, 20 and 260 mg/kg concentration levels which were supplied as sodium arsenate (Na<sub>3</sub>AsO<sub>4</sub>)<sub>.</sub> These experimental plants were grown in shade condition in a greenhouse. Plants were harvested at vigorous vegetative growth and fruit ripening stages, separately. Effects of As treatments on saponin content, and heterogeneity of monomers in the mixtures of notoginesenosides and ginsenosides, enzymatic activity and gene expression level of squalene synthetase were determined for rhizome and main root tissues. Results show that:(1) Of all the As treatments from the lowest to the highest concentration levels, the As content in both rhizome and main roots from As-treated plants was within the standard level for superior products derived from P. notoginseng. The content of notoginsenosides from all tissues except the main roots at fruit ripening stage, was 5% higher than the standard level specified in the Chinese Pharmacopoeia;(2) The treatment of As at 20 mg/kg led to an 3.5% - 183.9% increases in total notoginesenosides content in rhizome and main roots, respectively. Treatments with the highest As concentration at 260 mg/kg<sup> </sup>resulted in a significant decline in total notoginsenosides content, and lower enzymatic activity and gene expression levels of squalene synthetase;(3) Under As treatment conditions, the ratio of Rb1/Rg1 decreased but the ratio of (Rb1 + Rg1)/R1 increased in both rhizomes and main roots. Conclusively, this study demonstrated that low As concentration (20 - 80 展开更多
文摘As contamination is one of important factors to Panax notoginseng quality and safety. Saponin is one of important compounds with the medicinal values of P. notoginseng. The impact of soil As on production of saponin of P. notoginseng knew very little. This study was performed to determine content and heterogeneity of saponins from P. notoginseng and its mechanisms upon treatments with different concentration levels of As in soil. Plants of P. notoginseng were treated with arsenic [As (V)] at 0, 20, 80, 140, 20 and 260 mg/kg concentration levels which were supplied as sodium arsenate (Na<sub>3</sub>AsO<sub>4</sub>)<sub>.</sub> These experimental plants were grown in shade condition in a greenhouse. Plants were harvested at vigorous vegetative growth and fruit ripening stages, separately. Effects of As treatments on saponin content, and heterogeneity of monomers in the mixtures of notoginesenosides and ginsenosides, enzymatic activity and gene expression level of squalene synthetase were determined for rhizome and main root tissues. Results show that:(1) Of all the As treatments from the lowest to the highest concentration levels, the As content in both rhizome and main roots from As-treated plants was within the standard level for superior products derived from P. notoginseng. The content of notoginsenosides from all tissues except the main roots at fruit ripening stage, was 5% higher than the standard level specified in the Chinese Pharmacopoeia;(2) The treatment of As at 20 mg/kg led to an 3.5% - 183.9% increases in total notoginesenosides content in rhizome and main roots, respectively. Treatments with the highest As concentration at 260 mg/kg<sup> </sup>resulted in a significant decline in total notoginsenosides content, and lower enzymatic activity and gene expression levels of squalene synthetase;(3) Under As treatment conditions, the ratio of Rb1/Rg1 decreased but the ratio of (Rb1 + Rg1)/R1 increased in both rhizomes and main roots. Conclusively, this study demonstrated that low As concentration (20 - 80
