CRISPR/Cas9 （Clustered Regularly Interspaced Short Palin- dromic Repeats/CRISPR-associated Cas9 endonuclease）- mediated genome editing has revolutionized biological research and crop improvement because of its speci...CRISPR/Cas9 （Clustered Regularly Interspaced Short Palin- dromic Repeats/CRISPR-associated Cas9 endonuclease）- mediated genome editing has revolutionized biological research and crop improvement because of its specificity, simplicity, and versatility （reviewed in Komor et al., 2016a）.展开更多
The classification of the economically important genus Prunus L. sensu lato （s.L） is controversial due to the high levels of convergent or the parallel evolution of morphological characters. In the present study, ph...The classification of the economically important genus Prunus L. sensu lato （s.L） is controversial due to the high levels of convergent or the parallel evolution of morphological characters. In the present study, phylogenetic analyses of fifteen main segregates of Prunus s.I. represented by eighty-four species were conducted with maximum parsimony and Bayesian approaches using twelve chloroplast regions （atpB- rbcL, matK, ndhF, psbA-trnH, rbcL, rpL 16, rpoC1, rps16, trnS-G, trnL, trnL-F and ycfl） and three nuclear genes （ITS, s6pdh and Sbel） to explore their infrageneric used to develop a new, phylogeny-based classification relationships. The results of these analyses were of Prunus s.I. Our phylogenetic reconstructions resolved three main clades of Prunus s.I. with strong supports. We adopted a broad-sensed genus, Prunus, and recognised three subgenera corresponding to the three main clades： subgenus Padus, subgenus Cerasus and subgenus Prunus. Seven sections of subgenus Prunus were recognised. The dwarf cherries, which were previously assigned to subgenus Cerasus, were included in this subgenus Prunus. One new section name, Prunus L. subgenus Prunus section Persicae （T. T. yu ＆ L. T. Lu） S. L. Zhou and one new species name, Prunus tianshanica （Pojarkov） S. Shi, were proposed.展开更多
The programmed cell death-1(PD-1)/PD-ligand 1(PD-L1)pathway is critical for normal pregnancy by promoting regulatory T(Treg)cell development and inhibiting the Th17 response.However,the relationship between the PD-1/P...The programmed cell death-1(PD-1)/PD-ligand 1(PD-L1)pathway is critical for normal pregnancy by promoting regulatory T(Treg)cell development and inhibiting the Th17 response.However,the relationship between the PD-1/PD-L1 pathway and the Treg/Th17 imbalance in pre-eclampsia(PE)is an enigma.In this study,decreased PD-1 and PD-L1 expression and a Treg/Th17 imbalance were observed at the maternal-fetal interface in PE.The regulatory effects of the PD-1/PD-L1 pathway on the Treg and Th17 cell quantities were determined in vitro by targeting T-cell proliferation,differentiation and transdifferentiation.First,decreased PD-1 expression might contribute to a higher Th17 cell frequency by promoting proliferation in PE.Second,the percentages of Treg but not Th17 cells differentiated from peripheral naive CD4+T cells were increased by PD-L1 Fc administration.This effect was accompanied by decreased PI3K/AKT/m-TOR and increased PTEN mRNA expression and was completely reversed by PD-1 blockade.Finally,the percentage of IL-17-producing Treg cells increased and was positively associated with the Th17 cell frequency in PE.Increased RORγt and IL-17 but not Foxp3 and IL-10 mRNA expression by Treg cells was observed with PD-1 blockade.Similar findings occurred when Treg cells were exposed to IL-6/IL-23/IL-1βand were reversed by PD-L1 Fc.Taken together,our findings indicate that the PD-1/PD-L1 pathway contributes to the Treg/Th17 imbalance via‘one-two punch’approaches:(i)promoting Th17 cell proliferation,(ii)inhibiting Treg cell differentiation and(iii)enhancing Treg cell plasticity into Th17 cells in PE.The therapeutic value of PD-L1 Fc for PE treatment will be explored in the future.展开更多
The front of the Zoulang Nanshan Caledonian volcanic island arc zone in the northern Qilian Mountains is a forearc accretionary terrane, composed of multiple accretionary volcanic island arcs, flysch accretionary wedg...The front of the Zoulang Nanshan Caledonian volcanic island arc zone in the northern Qilian Mountains is a forearc accretionary terrane, composed of multiple accretionary volcanic island arcs, flysch accretionary wedges,high-pressure metamorphosed detachment zones and remnants of ophiolites. It resulted from the northeastward subduction of the Early Palaeozoic Qilan oceanic crust beneath the Alxa block. High-pressure metamorphism, which occurred during the subduction, progressed through three stages: the initial stage of medium T-high P,the main stage of temperature decrease and pressure increase, and the lag stage of pressure decrease and temperature increase. Finally the paper presents a retrotrench subduction dynamic model indicative of northward subduction of the central Qilian block and southward accretion of the Alxa block during the period of 450-500 Ma.展开更多
Finding the electromagnetic （EM） counterpart of binary compact star merger, especially the binary neutron star （BNS） merger, is critically important for gravitational wave （GW） astronomy, cosmology and fundament...Finding the electromagnetic （EM） counterpart of binary compact star merger, especially the binary neutron star （BNS） merger, is critically important for gravitational wave （GW） astronomy, cosmology and fundamental physics. On Aug. 17, 2017, Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart, GRB 170817A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope （HE） onboard Insight-HXMT （Hard X-ray Modulation Telescope） is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart （SSS17a/AT2017gfo） with very large collection area （M000 cm2） and microsecond time resolution in 0.2-5 MeV. In addition, Insight-HXMT quickly implemented a Target of Opportunity （TOO） observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy （0.2-5 MeV） radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817A. Meanwhile, Insight-HXMT/HE provides one of the most stringent constraints （-10-7 to 104 erg/cm2/s） for both GRB170817A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event.展开更多
As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray as...As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 Me V. It was designed to perform pointing, scanning and gamma-ray burst(GRB)observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed.Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results.展开更多
In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The m...In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment.展开更多
Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)m...Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was dec展开更多
Current studies have shown that the spatial-temporal graph convolutional network(STGCN)is effective for skeleton-based action recognition.However,for the existing STGCN-based methods,their temporal kernel size is usua...Current studies have shown that the spatial-temporal graph convolutional network(STGCN)is effective for skeleton-based action recognition.However,for the existing STGCN-based methods,their temporal kernel size is usually fixed over all layers,which makes them cannot fully exploit the temporal dependency between discontinuous frames and different sequence lengths.Besides,most of these methods use average pooling to obtain global graph feature from vertex features,resulting in losing much fine-grained information for action classification.To address these issues,in this work,the authors propose a novel spatial attentive and temporal dilated graph convolutional network(SATD-GCN).It contains two important components,that is,a spatial attention pooling module(SAP)and a temporal dilated graph convolution module(TDGC).Specifically,the SAP module can select the human body joints which are beneficial for action recognition by a self-attention mechanism and alleviates the influence of data redundancy and noise.The TDGC module can effectively extract the temporal features at different time scales,which is useful to improve the temporal perception field and enhance the robustness of the model to different motion speed and sequence length.Importantly,both the SAP module and the TDGC module can be easily integrated into the ST-GCN-based models,and significantly improve their performance.Extensive experiments on two large-scale benchmark datasets,that is,NTU-RGB+D and Kinetics-Skeleton,demonstrate that the authors’method achieves the state-of-the-art performance for skeleton-based action recognition.展开更多
WX-0593(Iruplinalkib)is a novel,highly selective oral ALK and ROS1 tyrosine kinase inhibitor(TKI).In this study,the safety,antitumor activity,and pharmacokinetics of WX-0593 were evaluated in advanced non-small cell l...WX-0593(Iruplinalkib)is a novel,highly selective oral ALK and ROS1 tyrosine kinase inhibitor(TKI).In this study,the safety,antitumor activity,and pharmacokinetics of WX-0593 were evaluated in advanced non-small cell lung cancer(NSCLC)patients with ALK or ROS1 rearrangement.In the dose-escalation phase and dose-expansion phase,patients were treated with WX-0593 until disease progression,unacceptable toxicity,or subject withdrawal.In the dose-escalation phase,the primary endpoints were maximum tolerated dose(MTD),dose-limiting toxicity(DLT),and safety assessed by investigators.In the dose-expansion phase,the primary endpoint was objective response rate(ORR)assessed by investigators.Between September 25,2017 and October 15,2018,a total of 153 patients received WX-0593 treatment.Two dose-limiting toxicities(DLTs)including one grade 3 QT interval prolonged and one grade 2 chronic heart failure were reported at the dose of 300 mg in one patient.MTD was not reached.Overall,140 of the 152(92%)patients experienced treatment-related adverse events(TRAEs)and 35 of the 152(23%)patients had TRAEs≥grade 3.The overall ORR was 59.3%(32 of 54)for the dose-escalation phase and 56.6%(56 of 99)for the dose-expansion phase.For patients who were ALK-rearranged and ALK TKI naive,the ORR were 81.0%(17 of 21)in the dose-escalation phase and 76.3%(29 of 38)in the dose-expansion phase,and for patients who previously received crizotinib as the only ALK TKI,the ORR were 38.1%(8 of 21)and 45.7%(21 of 46)for the two phases,respectively.For patients who were ROS1-rearranged,the ORR were 30.0%(3 of 10)in the dose-escalation phase and 44.4%(4 of 9)in the dose-expansion phase.WX-0593 showed favorable safety and promising antitumor activity in advanced NSCLC patients with ALK or ROS1 rearrangement.展开更多
In view of the variable parameters that affect the transient stability of electromagnetic torque and mechanical torque balance in AC-DC system,and the uncertainty of wind power in large-scale interconnection of wind f...In view of the variable parameters that affect the transient stability of electromagnetic torque and mechanical torque balance in AC-DC system,and the uncertainty of wind power in large-scale interconnection of wind farm.This paper proposes a linear parameter varying(LPV)robust feedback control method for transient stability of interconnected systems.The proposed LPV robust feedback control method uses the DC channel power control and the mechanical power in the interconnected system as the control target to improve the transient stability of the interconnected system with wind farm channel.Firstly,aiming at the strong nonlinear characteristics of the interconnected system,the power balance and the wind power output uncertainty in the transient process,the transient process is designed as a linear model of variable parameters.Then,the H∞robust output feedback controller is designed according to the LPV model.The transient stability control strategy topology and transfer function of the interconnected system are proposed.Finally,the proposed scheme is verified by an interconnected system formed by four equal-value grids through AC and DC lines in a digital simulation platform.The results show that the LPV robust feedback control model proposed in this paper has better response characteristics and transient stability control effects for interconnected systems with wind power weak sendingend system.展开更多
Prostate cancer tissue is composed of both cancer cells and host cells.The milieu of host components that compose the tumor is termed the tumor microenvironment(TME).Host cells can be those derived from the tissue in ...Prostate cancer tissue is composed of both cancer cells and host cells.The milieu of host components that compose the tumor is termed the tumor microenvironment(TME).Host cells can be those derived from the tissue in which the tumor originates(e.g.,fibroblasts and endothelial cells)or those recruited,through chemotactic or other factors,to the tumor(e.g.,circulating immune cells).Some immune cells are key players in the TME and represent a large proportion of non-tumor cells found within the tumor.Immune cells can have both anti-tumor and pro-tumor activity.In addition,crosstalk between prostate cancer cells and immune cells affects immune cell functions.In this review,we focus on immune cells and cytokines that contribute to tumor progression.We discuss T-regulatory and T helper17 cells and macrophages as key modulators in prostate cancer progression.In addition,we discuss the roles of interleukin-6 and receptor activator of nuclear factor kappa-B ligand in modulating prostate cancer progression.This review highlights the concept that immune cells and cytokines offer a potentially promising target for prostate cancer therapy.展开更多
Hydrogen molybdenum bronze (HxMoO3) can be electrodeposited on platinum and oxidized in two steps to the hydrogen molybdenum bronze with less amount of hydrogen HyMoO3 (y<x) and MoO3 when platinum electrode is cycl...Hydrogen molybdenum bronze (HxMoO3) can be electrodeposited on platinum and oxidized in two steps to the hydrogen molybdenum bronze with less amount of hydrogen HyMoO3 (y<x) and MoO3 when platinum electrode is cycled from -0.2 to 1.3V (vs. SCE) in 0.05 mol/L Na2MoO4 + 0.5 mol/L H2SO4 solution. During the formation of HxMoO3, the electrochemical reduction of molybdate existing in the form of polymolydate is reversible and is about a five-electron transfer reaction.展开更多
The Medium Energy X-ray telescope(ME) is one of the three main telescopes on board the Insight hard X-ray modulation telescope(Insight-HXMT) astronomy satellite. ME contains 1728 pixels of Si-PIN detectors sensitive i...The Medium Energy X-ray telescope(ME) is one of the three main telescopes on board the Insight hard X-ray modulation telescope(Insight-HXMT) astronomy satellite. ME contains 1728 pixels of Si-PIN detectors sensitive in 5-30 ke V with a total geometrical area of 952 cm^2. The application specific integrated circuit(ASIC) chip, VA32TA6, is used to achieve low power consumption and low readout noise. The collimators define three kinds of field of views(FOVs) for the telescope, 1°×4°, 4°×4°,and blocked ones. Combination of such FOVs can be used to estimate the in-orbit X-ray and particle background components.The energy resolution of ME is ~3 ke V at 17.8 ke V(FWHM) and the time resolution is 255 μs. In this paper, we introduce the design and performance of ME.展开更多
Thorium-234 and particle composition （organic matter, biogenic silica, carbonate and lithogenic component） were examined in the East China Sea （ECS） and the northern South China Sea （NSCS） in order to constrain ...Thorium-234 and particle composition （organic matter, biogenic silica, carbonate and lithogenic component） were examined in the East China Sea （ECS） and the northern South China Sea （NSCS） in order to constrain the particle types scavenging thorium isotopes. Good positive correlations between particulate organic matter （POM） or carbonate and thorium-234 in suspended particulate matter （SPM） indicates that POM and carbonate are efficient to scavenge thorium-234. No relationship between biogenic silica and thorium-234 suggests that geochemical behavior of thorium-234 may be not influenced by biogenic silica. A simple model was used to evaluate the affinity of thorium-234 to different particle components. The results show that POM is the most efficient scavenger for thorium-234 in the ECS and the NSCS, followed by carbonate. The authors＇ results lend support to the utility of thorium-234 as a proxy of POC and carbonate in the upper layer. However, the strong dependence of thorium scavenging on particle composition challenges thorium-230 as a constant flux proxy.展开更多
Metal-organic frameworks(MOFs)have shown significant potential as photocatalysts.It has been widely assumed that all catalytic active sites within MOFs are functional in photocatalytic reactions but for a three-dimens...Metal-organic frameworks(MOFs)have shown significant potential as photocatalysts.It has been widely assumed that all catalytic active sites within MOFs are functional in photocatalytic reactions but for a three-dimensional MOF,whether the internal catalytic active sites can effectively absorb light and actively contribute to photocatalytic reactions remains to be explored.In this context,we synthesized a two-dimensional nanosheet MOF(2D-MOF)and a three-dimensional bulk MOF(3D-MOF)composed of Zr^(6) clusters and tetracarboxylic porphyrin(TCPP)by the approach described in the literature.Re(bpy)(CO)^(3)Cl(bpy=2,2’-bipyridine),which has remarkable CO_(2) photoreduction ability,was introduced to the two MOFs to create two new photocatalysts 2D-MOF-Re and 3D-MOF-Re,respectively.Photocatalytic CO_(2) reduction experiments show that based on the equal number of catalytic active sites,the CO turnover number(TON)of 2D-MOF-Re reaches 27.8 in 6 h,which is 50 times that of 3D-MOF-Re.The result shows that certain catalytic active sites inside the bulk MOF are inactive due to the inability to absorb light.This study illuminates the potential of the dimensional reduction approach in the design of photocatalysts to exploit the capabilities fully.展开更多
The fractions of river runoff and sea-ice melted water in the Canada Basin in summer 2003 were determined by the salinity-δ18O system. The fraction of river runoff （fR） was high in the upper 50 m of the water colum...The fractions of river runoff and sea-ice melted water in the Canada Basin in summer 2003 were determined by the salinity-δ18O system. The fraction of river runoff （fR） was high in the upper 50 m of the water column and decreased with depth and latitude. The signals of the river runoffwere confined to water depths above 200 m. The total amount of river runoff in the Canada Basin was higher than that in other arctic seas, indicating that the Canada Basin is a main storage region for river runoff. The penetration depth of the sea-ice melted water was less than 50 m to the south of 78°N, while it was about 150 m to the north of 78°N. The total amount of sea-ice melted water was much higher to the north of 78°N than to the south of 78°N, indicating the sea-ice melted waters accumulated on the ice edge. The abundant sea-ice melted water on the ice edge was attributed to the earlier melted water in the southern Canada Basin and transported by the Beaufort Gyre or the reinforced melting of sea ice by solar radiation in the polynya.展开更多
OBJECTIVE: To investigate the influence of spleen deficiency on the epithelial barrier of jejunum and lungs in a rat model of spleen-deficiency and the effect and potential specialized pro-resolving mediators (SPMs) m...OBJECTIVE: To investigate the influence of spleen deficiency on the epithelial barrier of jejunum and lungs in a rat model of spleen-deficiency and the effect and potential specialized pro-resolving mediators (SPMs) mechanism of chiropractic manipulation. METHODS: Three-week-old male Sprague-Dawley rats were divided randomly into normal control group (n = 6), spleen-deficiency group (n = 5) and chiropractic group (n = 6). Spleen-deficiency model was induced in spleen-deficiency group and chiropractic group. Moreover, chiropractic manipulation was performed in chiropractic group. Four weeks later, systemic Th1/Th2 balance was evaluated by the ratio of plasma interferon (IFN)-γ/interleukin (IL)-4 levels by enzyme-linked immunosorbent assay (ELISA). Epithelial barrier integrity were assessed by the observation of morphological changes by hematoxylin-eosin staining and zonula occludens (ZO)-1 gene expressions by quantitative real time polymerase chain reaction in jejunum and lungs. Plasma resolvin D1 (RvD1) and lipoxin A4 (LXA4) levels were measures by ELISA for endogenous SPMs production. The levels of docosahexaenoic acid (DHA) and arachidonic acid (AA) in jejunum and lungs were also measured by HPLC-MS/ MS. RESULTS: Comparing with normal control group, spleen-deficiency group showed disrupted mucosa in jejunum, inflammatory condition in lungs, significantly decreased ratio of plasma IFN-γ/IL-4 levels and lower expressions of ZO-1 mRNA in both jejunum and lung tissues. Comparing with spleen-deficiency group, chiropractic group had less disrupted mucosa in jejunum and inflammatory condition in lungs, significantly increased systemic ratio of IFN-γ/IL-4 and expressions of ZO-1 mRNA in both jejunum and lung tissues. Chiropractic group had significantly enhanced plasma levels of RvD1 and LXA4, but had no significantly higher levels of DHA and AA in jejunum and lungs when comparing with spleen-deficiency group. CONCLUSION: Spleen deficiency caused systemic Th1/Th2 imbalance towards Th2 polarization and epithelial bar展开更多
Long-term excessive intake of nitrite(NO_(2)^(-))poses a great threat to human health,needing a simple and fast method to detect NO_(2)-in food.Herein,via a simple and feasible strategy,Mn/Yb/Er triple-doped CeO_(2) n...Long-term excessive intake of nitrite(NO_(2)^(-))poses a great threat to human health,needing a simple and fast method to detect NO_(2)-in food.Herein,via a simple and feasible strategy,Mn/Yb/Er triple-doped CeO_(2) nanozyme(Mn/Yb/Er/CeO_(2))was synthesized for highly sensitive ratiometric detection of nitrite.By doping Mn,Yb,Er into CeO_(2) lattice structure,Mn/Yb/Er/CeO_(2) nanozyme showed enhanced oxidase-like activity,obtaining a higher density of oxygen vacancy and a higher ratio of Ce^(3+)to Ce~(4+)than that of CeO_(2).The 3,3,5,5-tetramethylbenzidine(TMB)can be effectively oxidized by Mn/Yb/Er/CeO_(2) to produce the oxidized TMB(ox TMB),showing a significant absorption signal at 652 nm.Additionally,nitrite can react with ox TMB to produce yellow diazotized ox TMB,which is accompanied by an elevated absorption signal at 445 nm and a decreased absorption signal at 652 nm.Thus,based on the oxidase-mimetic activity of Mn/Yb/Er/CeO_(2) and the diazotization reaction between NO_(2)^(-)and ox TMB,a ratiometric colorimetric assay was established for NO_(2)^(-)detection in food.Furthermore,by integrating Mn/Yb/Er/CeO_(2) with a smartphone,a colorimetric smartphone-sensing platform was successfully fabricated for visualization and quantitative detection of NO_(2)^(-).Notably,this two-detection mode showed excellent sensitivity,selectivity,reliability and practicability in monitoring the NO_(2)^(-)in real samples,impling its great potential for food safety.展开更多
Plasma ignition technology has delivered good performance in the aerospace industry. In this study, a pre-combustion plasma jet igniter was designed, and its characteristics were examined from three aspects: the morph...Plasma ignition technology has delivered good performance in the aerospace industry. In this study, a pre-combustion plasma jet igniter was designed, and its characteristics were examined from three aspects: the morphology, temperature, and discharge characteristics and process of ignition. Images of the OH distribution were obtained by using an OH Planar Laser-Induced Fluorescence(OH-PLIF) experimental system. Results have shown that the proposed plasma jet had a higher OH concentration, longer length, and larger area than those of a traditional igniter. The stability of discharge of the igniter was improved as the equivalence ratio φ was increased, and reducing gas flow reduced the pulsation of the plasma jet. When the input current was increased from 15A to 35 A, the highest average temperature increased from 5127 K to 7987 K. An increase in the equivalence ratio reduced the region of arc ionization, but expanded the regions of the core combustion reaction and the outer flame. Herein, this study has obtained a deep understanding of the jet and ignition law and developed a new idea for the application of plasma in the ignition field.A pre-combustion plasma jet igniter can significantly improve the efficiency of ignition and shorten the ignition process compared with a traditional igniter.展开更多
文摘CRISPR/Cas9 （Clustered Regularly Interspaced Short Palin- dromic Repeats/CRISPR-associated Cas9 endonuclease）- mediated genome editing has revolutionized biological research and crop improvement because of its specificity, simplicity, and versatility （reviewed in Komor et al., 2016a）.
基金supported partly by National Natural Science Foundation of China (31270239)the National High-tech R&D Program (863 Program, 2012AA021602)
文摘The classification of the economically important genus Prunus L. sensu lato （s.L） is controversial due to the high levels of convergent or the parallel evolution of morphological characters. In the present study, phylogenetic analyses of fifteen main segregates of Prunus s.I. represented by eighty-four species were conducted with maximum parsimony and Bayesian approaches using twelve chloroplast regions （atpB- rbcL, matK, ndhF, psbA-trnH, rbcL, rpL 16, rpoC1, rps16, trnS-G, trnL, trnL-F and ycfl） and three nuclear genes （ITS, s6pdh and Sbel） to explore their infrageneric used to develop a new, phylogeny-based classification relationships. The results of these analyses were of Prunus s.I. Our phylogenetic reconstructions resolved three main clades of Prunus s.I. with strong supports. We adopted a broad-sensed genus, Prunus, and recognised three subgenera corresponding to the three main clades： subgenus Padus, subgenus Cerasus and subgenus Prunus. Seven sections of subgenus Prunus were recognised. The dwarf cherries, which were previously assigned to subgenus Cerasus, were included in this subgenus Prunus. One new section name, Prunus L. subgenus Prunus section Persicae （T. T. yu ＆ L. T. Lu） S. L. Zhou and one new species name, Prunus tianshanica （Pojarkov） S. Shi, were proposed.
基金This study was supported by research grants from the National Natural Science Foundation of China(NO.81471475).
文摘The programmed cell death-1(PD-1)/PD-ligand 1(PD-L1)pathway is critical for normal pregnancy by promoting regulatory T(Treg)cell development and inhibiting the Th17 response.However,the relationship between the PD-1/PD-L1 pathway and the Treg/Th17 imbalance in pre-eclampsia(PE)is an enigma.In this study,decreased PD-1 and PD-L1 expression and a Treg/Th17 imbalance were observed at the maternal-fetal interface in PE.The regulatory effects of the PD-1/PD-L1 pathway on the Treg and Th17 cell quantities were determined in vitro by targeting T-cell proliferation,differentiation and transdifferentiation.First,decreased PD-1 expression might contribute to a higher Th17 cell frequency by promoting proliferation in PE.Second,the percentages of Treg but not Th17 cells differentiated from peripheral naive CD4+T cells were increased by PD-L1 Fc administration.This effect was accompanied by decreased PI3K/AKT/m-TOR and increased PTEN mRNA expression and was completely reversed by PD-1 blockade.Finally,the percentage of IL-17-producing Treg cells increased and was positively associated with the Th17 cell frequency in PE.Increased RORγt and IL-17 but not Foxp3 and IL-10 mRNA expression by Treg cells was observed with PD-1 blockade.Similar findings occurred when Treg cells were exposed to IL-6/IL-23/IL-1βand were reversed by PD-L1 Fc.Taken together,our findings indicate that the PD-1/PD-L1 pathway contributes to the Treg/Th17 imbalance via‘one-two punch’approaches:(i)promoting Th17 cell proliferation,(ii)inhibiting Treg cell differentiation and(iii)enhancing Treg cell plasticity into Th17 cells in PE.The therapeutic value of PD-L1 Fc for PE treatment will be explored in the future.
基金This research is a project (No. 4870127)supported by the National Natural Science Foundation of China
文摘The front of the Zoulang Nanshan Caledonian volcanic island arc zone in the northern Qilian Mountains is a forearc accretionary terrane, composed of multiple accretionary volcanic island arcs, flysch accretionary wedges,high-pressure metamorphosed detachment zones and remnants of ophiolites. It resulted from the northeastward subduction of the Early Palaeozoic Qilan oceanic crust beneath the Alxa block. High-pressure metamorphism, which occurred during the subduction, progressed through three stages: the initial stage of medium T-high P,the main stage of temperature decrease and pressure increase, and the lag stage of pressure decrease and temperature increase. Finally the paper presents a retrotrench subduction dynamic model indicative of northward subduction of the central Qilian block and southward accretion of the Alxa block during the period of 450-500 Ma.
基金supported by the National Program on Key Research and Development Project(Grant No.2016YFA0400800)from the Ministry of Science and Technology of China(MOST)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB23040400)the Hundred Talent Program of Chinese Academy of Sciences,the National Natural Science Foundation of China(Grant Nos.11233001,11503027,11403026,11473027,and11733009)
文摘Finding the electromagnetic （EM） counterpart of binary compact star merger, especially the binary neutron star （BNS） merger, is critically important for gravitational wave （GW） astronomy, cosmology and fundamental physics. On Aug. 17, 2017, Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart, GRB 170817A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope （HE） onboard Insight-HXMT （Hard X-ray Modulation Telescope） is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart （SSS17a/AT2017gfo） with very large collection area （M000 cm2） and microsecond time resolution in 0.2-5 MeV. In addition, Insight-HXMT quickly implemented a Target of Opportunity （TOO） observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy （0.2-5 MeV） radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817A. Meanwhile, Insight-HXMT/HE provides one of the most stringent constraints （-10-7 to 104 erg/cm2/s） for both GRB170817A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event.
基金project funded by China National Space Administration(CNSA)and the Chinese Academy of Sciences(CAS)support from the National Key Research and Development Program of China(Grant No.2016YFA0400800)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant Nos.XDA04010202,XDA04010300,and XDB23040400)the National Natural Science Foundation of China(Grant Nos.U1838201,and U1838102).
文摘As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 Me V. It was designed to perform pointing, scanning and gamma-ray burst(GRB)observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed.Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results.
基金support of the Chinese Academy of Sciences through the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDA15020100)support by ASI, under the dedicated eXTP agreements and agreement ASI-INAF (Grant No. 2017-14-H.O.)+3 种基金by INAF and INFN under project REDSOXsupport from the Deutsche Zentrum für Luft- und Raumfahrt, the German Aerospce Center (DLR)support of Science Centre (Grant No. 2013/10/M/ST9/00729)support from MINECO (Grant No. ESP2017-82674-R) and FEDER funds
文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment.
基金supported by National Natural Science Foundation of China(NSFC)Key Project(No.81130046)NSFC(Nos.81272415 and 81171993)+1 种基金Guangxi Key Projects(No.2013GXNSFEA053004)Youth Science Foundation of Guangxi Medical University(No.GXMUYSF201539)
文摘Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was dec
基金National Key Research and Development Program of China,Grant/Award Number:2018YFB1600600。
文摘Current studies have shown that the spatial-temporal graph convolutional network(STGCN)is effective for skeleton-based action recognition.However,for the existing STGCN-based methods,their temporal kernel size is usually fixed over all layers,which makes them cannot fully exploit the temporal dependency between discontinuous frames and different sequence lengths.Besides,most of these methods use average pooling to obtain global graph feature from vertex features,resulting in losing much fine-grained information for action classification.To address these issues,in this work,the authors propose a novel spatial attentive and temporal dilated graph convolutional network(SATD-GCN).It contains two important components,that is,a spatial attention pooling module(SAP)and a temporal dilated graph convolution module(TDGC).Specifically,the SAP module can select the human body joints which are beneficial for action recognition by a self-attention mechanism and alleviates the influence of data redundancy and noise.The TDGC module can effectively extract the temporal features at different time scales,which is useful to improve the temporal perception field and enhance the robustness of the model to different motion speed and sequence length.Importantly,both the SAP module and the TDGC module can be easily integrated into the ST-GCN-based models,and significantly improve their performance.Extensive experiments on two large-scale benchmark datasets,that is,NTU-RGB+D and Kinetics-Skeleton,demonstrate that the authors’method achieves the state-of-the-art performance for skeleton-based action recognition.
基金This study(NCT03389815)was funded by Qilu Pharmaceutical Co.,Ltd.and also supported in part by China National Major Project for New Drug Innovation(2017ZX09304015)Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(CIFMS)(2016-I2M-1-001).
文摘WX-0593(Iruplinalkib)is a novel,highly selective oral ALK and ROS1 tyrosine kinase inhibitor(TKI).In this study,the safety,antitumor activity,and pharmacokinetics of WX-0593 were evaluated in advanced non-small cell lung cancer(NSCLC)patients with ALK or ROS1 rearrangement.In the dose-escalation phase and dose-expansion phase,patients were treated with WX-0593 until disease progression,unacceptable toxicity,or subject withdrawal.In the dose-escalation phase,the primary endpoints were maximum tolerated dose(MTD),dose-limiting toxicity(DLT),and safety assessed by investigators.In the dose-expansion phase,the primary endpoint was objective response rate(ORR)assessed by investigators.Between September 25,2017 and October 15,2018,a total of 153 patients received WX-0593 treatment.Two dose-limiting toxicities(DLTs)including one grade 3 QT interval prolonged and one grade 2 chronic heart failure were reported at the dose of 300 mg in one patient.MTD was not reached.Overall,140 of the 152(92%)patients experienced treatment-related adverse events(TRAEs)and 35 of the 152(23%)patients had TRAEs≥grade 3.The overall ORR was 59.3%(32 of 54)for the dose-escalation phase and 56.6%(56 of 99)for the dose-expansion phase.For patients who were ALK-rearranged and ALK TKI naive,the ORR were 81.0%(17 of 21)in the dose-escalation phase and 76.3%(29 of 38)in the dose-expansion phase,and for patients who previously received crizotinib as the only ALK TKI,the ORR were 38.1%(8 of 21)and 45.7%(21 of 46)for the two phases,respectively.For patients who were ROS1-rearranged,the ORR were 30.0%(3 of 10)in the dose-escalation phase and 44.4%(4 of 9)in the dose-expansion phase.WX-0593 showed favorable safety and promising antitumor activity in advanced NSCLC patients with ALK or ROS1 rearrangement.
基金This study was supported in part by the National Key R&D Program of China(2017YFB0902100).
文摘In view of the variable parameters that affect the transient stability of electromagnetic torque and mechanical torque balance in AC-DC system,and the uncertainty of wind power in large-scale interconnection of wind farm.This paper proposes a linear parameter varying(LPV)robust feedback control method for transient stability of interconnected systems.The proposed LPV robust feedback control method uses the DC channel power control and the mechanical power in the interconnected system as the control target to improve the transient stability of the interconnected system with wind farm channel.Firstly,aiming at the strong nonlinear characteristics of the interconnected system,the power balance and the wind power output uncertainty in the transient process,the transient process is designed as a linear model of variable parameters.Then,the H∞robust output feedback controller is designed according to the LPV model.The transient stability control strategy topology and transfer function of the interconnected system are proposed.Finally,the proposed scheme is verified by an interconnected system formed by four equal-value grids through AC and DC lines in a digital simulation platform.The results show that the LPV robust feedback control model proposed in this paper has better response characteristics and transient stability control effects for interconnected systems with wind power weak sendingend system.
基金supported by National Cancer Institute Grants(Nos.P01 CA093900 and R01 CA190554)National Natural Science Foundation of China(NSFC)Key Projects(Nos.81130046+1 种基金NSFC 81171993 and 81272415)Guangxi Key Project(No.2013GXNSFEA053004)
文摘Prostate cancer tissue is composed of both cancer cells and host cells.The milieu of host components that compose the tumor is termed the tumor microenvironment(TME).Host cells can be those derived from the tissue in which the tumor originates(e.g.,fibroblasts and endothelial cells)or those recruited,through chemotactic or other factors,to the tumor(e.g.,circulating immune cells).Some immune cells are key players in the TME and represent a large proportion of non-tumor cells found within the tumor.Immune cells can have both anti-tumor and pro-tumor activity.In addition,crosstalk between prostate cancer cells and immune cells affects immune cell functions.In this review,we focus on immune cells and cytokines that contribute to tumor progression.We discuss T-regulatory and T helper17 cells and macrophages as key modulators in prostate cancer progression.In addition,we discuss the roles of interleukin-6 and receptor activator of nuclear factor kappa-B ligand in modulating prostate cancer progression.This review highlights the concept that immune cells and cytokines offer a potentially promising target for prostate cancer therapy.
文摘Hydrogen molybdenum bronze (HxMoO3) can be electrodeposited on platinum and oxidized in two steps to the hydrogen molybdenum bronze with less amount of hydrogen HyMoO3 (y<x) and MoO3 when platinum electrode is cycled from -0.2 to 1.3V (vs. SCE) in 0.05 mol/L Na2MoO4 + 0.5 mol/L H2SO4 solution. During the formation of HxMoO3, the electrochemical reduction of molybdate existing in the form of polymolydate is reversible and is about a five-electron transfer reaction.
基金the Strategic Priority Research Program on Space Science,the Chinese Academy of Sciences(Grant No.XDA040102).
文摘The Medium Energy X-ray telescope(ME) is one of the three main telescopes on board the Insight hard X-ray modulation telescope(Insight-HXMT) astronomy satellite. ME contains 1728 pixels of Si-PIN detectors sensitive in 5-30 ke V with a total geometrical area of 952 cm^2. The application specific integrated circuit(ASIC) chip, VA32TA6, is used to achieve low power consumption and low readout noise. The collimators define three kinds of field of views(FOVs) for the telescope, 1°×4°, 4°×4°,and blocked ones. Combination of such FOVs can be used to estimate the in-orbit X-ray and particle background components.The energy resolution of ME is ~3 ke V at 17.8 ke V(FWHM) and the time resolution is 255 μs. In this paper, we introduce the design and performance of ME.
基金The National Natural Science Foundation of China under contract Nos 40606022 and 90411016 the National Key Basic Research Special Foundation Program of China under contract No 2005CB422305
文摘Thorium-234 and particle composition （organic matter, biogenic silica, carbonate and lithogenic component） were examined in the East China Sea （ECS） and the northern South China Sea （NSCS） in order to constrain the particle types scavenging thorium isotopes. Good positive correlations between particulate organic matter （POM） or carbonate and thorium-234 in suspended particulate matter （SPM） indicates that POM and carbonate are efficient to scavenge thorium-234. No relationship between biogenic silica and thorium-234 suggests that geochemical behavior of thorium-234 may be not influenced by biogenic silica. A simple model was used to evaluate the affinity of thorium-234 to different particle components. The results show that POM is the most efficient scavenger for thorium-234 in the ECS and the NSCS, followed by carbonate. The authors＇ results lend support to the utility of thorium-234 as a proxy of POC and carbonate in the upper layer. However, the strong dependence of thorium scavenging on particle composition challenges thorium-230 as a constant flux proxy.
文摘Metal-organic frameworks(MOFs)have shown significant potential as photocatalysts.It has been widely assumed that all catalytic active sites within MOFs are functional in photocatalytic reactions but for a three-dimensional MOF,whether the internal catalytic active sites can effectively absorb light and actively contribute to photocatalytic reactions remains to be explored.In this context,we synthesized a two-dimensional nanosheet MOF(2D-MOF)and a three-dimensional bulk MOF(3D-MOF)composed of Zr^(6) clusters and tetracarboxylic porphyrin(TCPP)by the approach described in the literature.Re(bpy)(CO)^(3)Cl(bpy=2,2’-bipyridine),which has remarkable CO_(2) photoreduction ability,was introduced to the two MOFs to create two new photocatalysts 2D-MOF-Re and 3D-MOF-Re,respectively.Photocatalytic CO_(2) reduction experiments show that based on the equal number of catalytic active sites,the CO turnover number(TON)of 2D-MOF-Re reaches 27.8 in 6 h,which is 50 times that of 3D-MOF-Re.The result shows that certain catalytic active sites inside the bulk MOF are inactive due to the inability to absorb light.This study illuminates the potential of the dimensional reduction approach in the design of photocatalysts to exploit the capabilities fully.
基金The Chinese Polar Environment Comprehensive Investigation and Assessment Programs under contract No.CHINARE2014-03-04-03the National Natural Science Foundation of China under contract No.41125020+1 种基金a special scientific research project for public welfare supported by the State Oceanic Administration under contract No.201105022-4the 4th Chinese Arctic Research Program
文摘The fractions of river runoff and sea-ice melted water in the Canada Basin in summer 2003 were determined by the salinity-δ18O system. The fraction of river runoff （fR） was high in the upper 50 m of the water column and decreased with depth and latitude. The signals of the river runoffwere confined to water depths above 200 m. The total amount of river runoff in the Canada Basin was higher than that in other arctic seas, indicating that the Canada Basin is a main storage region for river runoff. The penetration depth of the sea-ice melted water was less than 50 m to the south of 78°N, while it was about 150 m to the north of 78°N. The total amount of sea-ice melted water was much higher to the north of 78°N than to the south of 78°N, indicating the sea-ice melted waters accumulated on the ice edge. The abundant sea-ice melted water on the ice edge was attributed to the earlier melted water in the southern Canada Basin and transported by the Beaufort Gyre or the reinforced melting of sea ice by solar radiation in the polynya.
基金Supported by the National Natural Science Foundationfunded Project:the Mechanism of Spine-pinching Manipulation via Inflammation Regulation of SPMs on Prevention and Treatment of Spleen-deficiency Asthma(No.81774446)
文摘OBJECTIVE: To investigate the influence of spleen deficiency on the epithelial barrier of jejunum and lungs in a rat model of spleen-deficiency and the effect and potential specialized pro-resolving mediators (SPMs) mechanism of chiropractic manipulation. METHODS: Three-week-old male Sprague-Dawley rats were divided randomly into normal control group (n = 6), spleen-deficiency group (n = 5) and chiropractic group (n = 6). Spleen-deficiency model was induced in spleen-deficiency group and chiropractic group. Moreover, chiropractic manipulation was performed in chiropractic group. Four weeks later, systemic Th1/Th2 balance was evaluated by the ratio of plasma interferon (IFN)-γ/interleukin (IL)-4 levels by enzyme-linked immunosorbent assay (ELISA). Epithelial barrier integrity were assessed by the observation of morphological changes by hematoxylin-eosin staining and zonula occludens (ZO)-1 gene expressions by quantitative real time polymerase chain reaction in jejunum and lungs. Plasma resolvin D1 (RvD1) and lipoxin A4 (LXA4) levels were measures by ELISA for endogenous SPMs production. The levels of docosahexaenoic acid (DHA) and arachidonic acid (AA) in jejunum and lungs were also measured by HPLC-MS/ MS. RESULTS: Comparing with normal control group, spleen-deficiency group showed disrupted mucosa in jejunum, inflammatory condition in lungs, significantly decreased ratio of plasma IFN-γ/IL-4 levels and lower expressions of ZO-1 mRNA in both jejunum and lung tissues. Comparing with spleen-deficiency group, chiropractic group had less disrupted mucosa in jejunum and inflammatory condition in lungs, significantly increased systemic ratio of IFN-γ/IL-4 and expressions of ZO-1 mRNA in both jejunum and lung tissues. Chiropractic group had significantly enhanced plasma levels of RvD1 and LXA4, but had no significantly higher levels of DHA and AA in jejunum and lungs when comparing with spleen-deficiency group. CONCLUSION: Spleen deficiency caused systemic Th1/Th2 imbalance towards Th2 polarization and epithelial bar
基金supported by the National Natural Science Foundation of China(Nos.22004111 and 21974125)the Tackle Key Problems in Science and Technology Project of Henan Province,China(No.222102310386)+3 种基金China Postdoctoral Science Foundation(No.2020M682327)Henan Postdoctoral Foundation(No.202002009)Program for Innovative Research Team(in Science and Technology)in University of Henan Province(No.22TRTSTHN002)Excellent Youth Foundation of Henan Scientific Committee(No.232300421021)。
文摘Long-term excessive intake of nitrite(NO_(2)^(-))poses a great threat to human health,needing a simple and fast method to detect NO_(2)-in food.Herein,via a simple and feasible strategy,Mn/Yb/Er triple-doped CeO_(2) nanozyme(Mn/Yb/Er/CeO_(2))was synthesized for highly sensitive ratiometric detection of nitrite.By doping Mn,Yb,Er into CeO_(2) lattice structure,Mn/Yb/Er/CeO_(2) nanozyme showed enhanced oxidase-like activity,obtaining a higher density of oxygen vacancy and a higher ratio of Ce^(3+)to Ce~(4+)than that of CeO_(2).The 3,3,5,5-tetramethylbenzidine(TMB)can be effectively oxidized by Mn/Yb/Er/CeO_(2) to produce the oxidized TMB(ox TMB),showing a significant absorption signal at 652 nm.Additionally,nitrite can react with ox TMB to produce yellow diazotized ox TMB,which is accompanied by an elevated absorption signal at 445 nm and a decreased absorption signal at 652 nm.Thus,based on the oxidase-mimetic activity of Mn/Yb/Er/CeO_(2) and the diazotization reaction between NO_(2)^(-)and ox TMB,a ratiometric colorimetric assay was established for NO_(2)^(-)detection in food.Furthermore,by integrating Mn/Yb/Er/CeO_(2) with a smartphone,a colorimetric smartphone-sensing platform was successfully fabricated for visualization and quantitative detection of NO_(2)^(-).Notably,this two-detection mode showed excellent sensitivity,selectivity,reliability and practicability in monitoring the NO_(2)^(-)in real samples,impling its great potential for food safety.
基金co-supported by the National Natural Science Foundation of China(No.52306123)the Postdoctoral Science Foundation,China(No.2023M734277)the Natural Science Basic Research Program of Shaanxi,China(No.2021JQ-046)。
文摘Plasma ignition technology has delivered good performance in the aerospace industry. In this study, a pre-combustion plasma jet igniter was designed, and its characteristics were examined from three aspects: the morphology, temperature, and discharge characteristics and process of ignition. Images of the OH distribution were obtained by using an OH Planar Laser-Induced Fluorescence(OH-PLIF) experimental system. Results have shown that the proposed plasma jet had a higher OH concentration, longer length, and larger area than those of a traditional igniter. The stability of discharge of the igniter was improved as the equivalence ratio φ was increased, and reducing gas flow reduced the pulsation of the plasma jet. When the input current was increased from 15A to 35 A, the highest average temperature increased from 5127 K to 7987 K. An increase in the equivalence ratio reduced the region of arc ionization, but expanded the regions of the core combustion reaction and the outer flame. Herein, this study has obtained a deep understanding of the jet and ignition law and developed a new idea for the application of plasma in the ignition field.A pre-combustion plasma jet igniter can significantly improve the efficiency of ignition and shorten the ignition process compared with a traditional igniter.
