A long-term analysis of signal-in-space range error (SISRE) is presented for all healthy Galileo satellites, and the first pair of full operational capability satellites in wrong elliptical orbits. Both orbit and cloc...A long-term analysis of signal-in-space range error (SISRE) is presented for all healthy Galileo satellites, and the first pair of full operational capability satellites in wrong elliptical orbits. Both orbit and clock errors for Galileo show an obvious convergence trend over time. The annual statistical analyses show that the average root mean squares (RMSs) of SISRE for the Galileo constellation are 0.58 m (2015), 0.29 m (2016), 0.23 m (2017), and 0.22 m (2018). Currently, the accuracy of the Galileo signal-in-space is superior to that of the global positioning system (GPS) Block IIF (0.35 m). In addition, the orbit error accounts for the majority of Galileo SISRE, while the clock error accounts for approximately one-third of SISRE due to the high stability of the onboard atomic clock. Single point positioning results show that Galileo achieves an accuracy of 2-3 m, which is comparable to that of GPS despite the smaller number of satellites and worse geometry. Interestingly, the vertical accuracy of Galileo, which uses the NeQuick ionospheric model, is higher than that of GPS. Positioning with single frequency E1 and E5 show a higher precision than E5a and E5b signals. Regarding precise point positioning (PPP), the results indicate that a comparable positioning accuracy can be achieved among different stations with the current Galileo constellation. For static PPP, the RMS values of Galileo-only solutions are within 1 cm horizontally, and the vertical RMSs are mostly within 2 cm horizontally. For kinematic PPP, the RMSs of Galileo-only solutions are mostly within 4 cm horizontally and 6 cm vertically.展开更多
Triticum urartu is the progenitor of the A subgenome in tetraploid and hexaploid wheat.Uncovering the landscape of genetic variations in T.urartu will help us understand the evolutionary and polyploid characteristics ...Triticum urartu is the progenitor of the A subgenome in tetraploid and hexaploid wheat.Uncovering the landscape of genetic variations in T.urartu will help us understand the evolutionary and polyploid characteristics of wheat.Here,we investigated the population genomics of T.urartu by genome-wide sequencing of 59 representative accessions collected around the world.A total of 42.2 million highquality single-nucleotide polymorphisms and 3 million insertions and deletions were obtained by mapping reads to the reference genome.The ancient T.urartu population experienced a significant reduction in effective population size(Ne)from3000000 to140000 and subsequently split into eastern Mediterranean coastal and Mesopotamian-Transcaucasian populations during the Younger Dryas period.A map of allelic drift paths displayed splits and mixtures between different geographic groups,and a strong genetic drift towards hexaploid wheat was also observed,indicating that the direct donor of the A subgenome originated from northwestern Syria.Genetic changes were revealed between the eastern Mediterranean coastal and Mesopotamian-Transcaucasian populations in genes orthologous to those regulating plant development and stress responses.A genome-wide association study identified two single-nucleotide polymorphisms in the exonic regions of the SEMI-DWARF 37 ortholog that corresponded to the different T.urartu ecotype groups.Our study provides novel insights into the origin and genetic legacy of the A subgenome in polyploid wheat and contributes a gene repertoire for genomicsenabled improvements in wheat breeding.展开更多
This paper presents the TDS-1 GNSS reflectometry wind Geophysical Model Function(GMF)response to GPS block types.The observables were extracted from Delay Doppler Maps(DDMs)after taking the receiver antenna gains effe...This paper presents the TDS-1 GNSS reflectometry wind Geophysical Model Function(GMF)response to GPS block types.The observables were extracted from Delay Doppler Maps(DDMs)after taking the receiver antenna gains effects and GNSS-R geometry effects into account.Since the DDM is affected by GPS EffectiveIsotropic Radiated Power(EIRP),we first investigate the sensitivity of observables to the GPS block.Additionally,the observables at high SNRs are more sensitive to wind speed,but the spatial coverage at high signal to noise ratios(SNRs)is lower,while DDMs at low SNRs have the opposite characteristics.To balance the accuracy and spatial coverage,the DDM datasets are divided into two parts:high SNR(>0 dB)and low SNR(>−10 dB and≤0 dB)to develop wind GMF.Then,the influences of GPS block on wind speed retrieval both at high and low SNR is analyzed.Results show that the block types have impacts on wind GMF and the use of a prior GPS block can contribute to a better wind speed retrieval both at high and low SNR.Compared with ASCAT,the Root Mean Square Error(RMSE)value of wind speed retrieval at high and low SNR are 2.19 m/s and 3.13 m/s,respectively,when all TDS data are processed without distinguishing GPS block types.However,if the TDS data are separately processed and used to develop wind GMF through different blocks,both the accuracy and correlation coefficient can be improved to some extent.Finally,the influence of significant height of the swell(Hs)on SNR observables is analyzed,and it is demonstrated that there is no obvious linear or nonlinear relationship between them.展开更多
Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy.However,long-term super-resol...Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy.However,long-term super-resolution imaging of mitochondria is still challenging due to the lack of photostable fluorescent probes and stable mitochondria-specific markers which are not affected by the changes of mitochondrial membrane potential.Here,we introduce a method for long-term imaging mitochondrial dynamic through the SNAP-tag fluorogenic probe based on 4-azetidinyl-naphthalimide derivatives.Using structured illumination microscopy(SIM),we observed the fusion and fission of mitochondria over a course of 16 min at 109 nm resolution.Furthermore,the interactions as well as fusion between mitochondria and lysosomes were studied during mitophagy at the nanoscale.Convincingly,the combination of SNAP-tag fluorogenic probes and super-resolution fluorescence microscopy will offer a new way to monitor dynamic mitochondria in living cells.展开更多
基金the National Natural Science Foundation of China(No.41774034)the National Key Research and Development Program of China(No.2016YFB0501803,No.2017YFB0503402).
文摘A long-term analysis of signal-in-space range error (SISRE) is presented for all healthy Galileo satellites, and the first pair of full operational capability satellites in wrong elliptical orbits. Both orbit and clock errors for Galileo show an obvious convergence trend over time. The annual statistical analyses show that the average root mean squares (RMSs) of SISRE for the Galileo constellation are 0.58 m (2015), 0.29 m (2016), 0.23 m (2017), and 0.22 m (2018). Currently, the accuracy of the Galileo signal-in-space is superior to that of the global positioning system (GPS) Block IIF (0.35 m). In addition, the orbit error accounts for the majority of Galileo SISRE, while the clock error accounts for approximately one-third of SISRE due to the high stability of the onboard atomic clock. Single point positioning results show that Galileo achieves an accuracy of 2-3 m, which is comparable to that of GPS despite the smaller number of satellites and worse geometry. Interestingly, the vertical accuracy of Galileo, which uses the NeQuick ionospheric model, is higher than that of GPS. Positioning with single frequency E1 and E5 show a higher precision than E5a and E5b signals. Regarding precise point positioning (PPP), the results indicate that a comparable positioning accuracy can be achieved among different stations with the current Galileo constellation. For static PPP, the RMS values of Galileo-only solutions are within 1 cm horizontally, and the vertical RMSs are mostly within 2 cm horizontally. For kinematic PPP, the RMSs of Galileo-only solutions are mostly within 4 cm horizontally and 6 cm vertically.
基金This research was financially supported by the National Natural Science Foundation of China(31871617)the Ministry of Science and Technology of the People’s Republic of China(2016YFD0102002 and 2011AA100104).
文摘Triticum urartu is the progenitor of the A subgenome in tetraploid and hexaploid wheat.Uncovering the landscape of genetic variations in T.urartu will help us understand the evolutionary and polyploid characteristics of wheat.Here,we investigated the population genomics of T.urartu by genome-wide sequencing of 59 representative accessions collected around the world.A total of 42.2 million highquality single-nucleotide polymorphisms and 3 million insertions and deletions were obtained by mapping reads to the reference genome.The ancient T.urartu population experienced a significant reduction in effective population size(Ne)from3000000 to140000 and subsequently split into eastern Mediterranean coastal and Mesopotamian-Transcaucasian populations during the Younger Dryas period.A map of allelic drift paths displayed splits and mixtures between different geographic groups,and a strong genetic drift towards hexaploid wheat was also observed,indicating that the direct donor of the A subgenome originated from northwestern Syria.Genetic changes were revealed between the eastern Mediterranean coastal and Mesopotamian-Transcaucasian populations in genes orthologous to those regulating plant development and stress responses.A genome-wide association study identified two single-nucleotide polymorphisms in the exonic regions of the SEMI-DWARF 37 ortholog that corresponded to the different T.urartu ecotype groups.Our study provides novel insights into the origin and genetic legacy of the A subgenome in polyploid wheat and contributes a gene repertoire for genomicsenabled improvements in wheat breeding.
基金supported by the Funds for Creative Research Groups of China[Grant no.41721003]the National Natural Science Foundation of China[Grant nos.41825009 and 41774034].
文摘This paper presents the TDS-1 GNSS reflectometry wind Geophysical Model Function(GMF)response to GPS block types.The observables were extracted from Delay Doppler Maps(DDMs)after taking the receiver antenna gains effects and GNSS-R geometry effects into account.Since the DDM is affected by GPS EffectiveIsotropic Radiated Power(EIRP),we first investigate the sensitivity of observables to the GPS block.Additionally,the observables at high SNRs are more sensitive to wind speed,but the spatial coverage at high signal to noise ratios(SNRs)is lower,while DDMs at low SNRs have the opposite characteristics.To balance the accuracy and spatial coverage,the DDM datasets are divided into two parts:high SNR(>0 dB)and low SNR(>−10 dB and≤0 dB)to develop wind GMF.Then,the influences of GPS block on wind speed retrieval both at high and low SNR is analyzed.Results show that the block types have impacts on wind GMF and the use of a prior GPS block can contribute to a better wind speed retrieval both at high and low SNR.Compared with ASCAT,the Root Mean Square Error(RMSE)value of wind speed retrieval at high and low SNR are 2.19 m/s and 3.13 m/s,respectively,when all TDS data are processed without distinguishing GPS block types.However,if the TDS data are separately processed and used to develop wind GMF through different blocks,both the accuracy and correlation coefficient can be improved to some extent.Finally,the influence of significant height of the swell(Hs)on SNR observables is analyzed,and it is demonstrated that there is no obvious linear or nonlinear relationship between them.
基金the National Natural Science Foundation of China(Nos.21878286,21576043,21878286)Dalian Institute of Chemical Physics,Chinese Academy of Sciences(Nos.I201938,ZZBS201805)。
文摘Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy.However,long-term super-resolution imaging of mitochondria is still challenging due to the lack of photostable fluorescent probes and stable mitochondria-specific markers which are not affected by the changes of mitochondrial membrane potential.Here,we introduce a method for long-term imaging mitochondrial dynamic through the SNAP-tag fluorogenic probe based on 4-azetidinyl-naphthalimide derivatives.Using structured illumination microscopy(SIM),we observed the fusion and fission of mitochondria over a course of 16 min at 109 nm resolution.Furthermore,the interactions as well as fusion between mitochondria and lysosomes were studied during mitophagy at the nanoscale.Convincingly,the combination of SNAP-tag fluorogenic probes and super-resolution fluorescence microscopy will offer a new way to monitor dynamic mitochondria in living cells.
