CHDTEPDB(URL:http://chdtepdb.com/)is a manually integrated database for congenital heart disease(CHD)that stores the expression profiling data of CHD derived from published papers,aiming to provide rich resources for i...CHDTEPDB(URL:http://chdtepdb.com/)is a manually integrated database for congenital heart disease(CHD)that stores the expression profiling data of CHD derived from published papers,aiming to provide rich resources for investigating a deeper correlation between human CHD and aberrant transcriptome expression.The develop-ment of human diseases involves important regulatory roles of RNAs,and expression profiling data can reflect the underlying etiology of inherited diseases.Hence,collecting and compiling expression profiling data is of critical significance for a comprehensive understanding of the mechanisms and functions that underpin genetic diseases.CHDTEPDB stores the expression profiles of over 200 sets of 7 types of CHD and provides users with more convenient basic analytical functions.Due to the differences in clinical indicators such as disease type and unavoidable detection errors among various datasets,users are able to customize their selection of corresponding data for personalized analysis.Moreover,we provide a submission page for researchers to submit their own data so that increasing expression profiles as well as some other histological data could be supplemented to the database.CHDTEPDB is a user-friendly interface that allows users to quickly browse,retrieve,download,and analyze their target samples.CHDTEPDB will significantly improve the current knowledge of expression profiling data in CHD and has the potential to be exploited as an important tool for future research on the disease.展开更多
Triple negative breast cancer(TNBC) is an aggressive subtype of breast cancer that currently lacks effective biomarkers and therapeutic targets required to investigate the diagnosis and treatment of TNBC. Here we perf...Triple negative breast cancer(TNBC) is an aggressive subtype of breast cancer that currently lacks effective biomarkers and therapeutic targets required to investigate the diagnosis and treatment of TNBC. Here we performed a comprehensive differential analysis of 165 TNBC samples by integrating RNA-seq data of breast tumor tissues and adjacent normal tissues from both our cohort and The Cancer Genome Atlas(TCGA). Pathway enrichment analysis was conducted to evaluate the biological function of TNBC-specific expressed genes. Further multivariate Cox proportional hazard regression was performed to evaluate the effect of these genes on TNBC prognosis. In this report, we identified a total of 148 TNBC-specific expressed genes that were primarily enriched in mammary gland morphogenesis and hormone levels related pathways, suggesting that mammary gland morphogenesis might play a unique role in TNBC patients differing from other breast cancer types. Further survival analysis revealed that nine genes(FSIP1, ADCY5, FSD1, HMSD, CMTM5, AFF3, CYP2 A7, ATP1 A2,and C11 orf86) were significantly associated with the prognosis of TNBC patients, while three of them(ADCY5,CYP2 A7, and ATP1 A2) were involved in the hormone-related pathways. These findings indicated the vital role of the hormone-related genes in TNBC tumorigenesis and may provide some independent prognostic markers as well as novel therapeutic targets for TNBC.展开更多
Platinum telluride(PtTe_(2)),a member of metallic transition metal dichalcogenides,provides a new platform for investigating various properties such as type-II Dirac fermions,topological superconductivity,and wide-ban...Platinum telluride(PtTe_(2)),a member of metallic transition metal dichalcogenides,provides a new platform for investigating various properties such as type-II Dirac fermions,topological superconductivity,and wide-band photodetection.However,the study of PtTe_(2)is largely limited to exfoliated flakes,and its direct synthesis remains challenging.Herein,we report the controllable synthesis of highly crystalline 2D PtTe_(2)crystals with tunable morphology and thickness via chemical vapor deposition(CVD)growth on Au substrate.By adjusting Te amount and substrate temperature,anisotropic and isotropic growth modes of PtTe_(2)were realized on the solid and molten Au substrates,respectively.The domain size of PtTe_(2)crystal was achieved up to 30μm,and its thickness can be tuned from 5.6 to 50 nm via controlling the growth time.Furthermore,a metal–PtTe_(2)–metal structural device was fabricated to validate the wide-band terahertz(THz)photodetection from 0.04 to 0.3 THz at room temperature.Owing to the high crystallinity of PtTe_(2)crystal,the photodetector acquires high responsivity(30–250 mA W-1 from 0.12 to 0.3 THz),fast response rate(rise time:7μs,decay time:8μs),and high-quality imaging ability.Our work demonstrates the feasibility for realistic exploitation of high-performing photodetection system at THz band based on the CVDgrown 2D Dirac semimetal materials.展开更多
大型出生队列是持续、动态地收集个体生命早期暴露信息,探讨暴露与生命远期健康结局因果关联的一种重要的队列研究类型。但由于其设计复杂、实施难度大,如何保证出生队列建设的高质量高效率是国内外流行病学研究者面临的主要挑战。2016...大型出生队列是持续、动态地收集个体生命早期暴露信息,探讨暴露与生命远期健康结局因果关联的一种重要的队列研究类型。但由于其设计复杂、实施难度大,如何保证出生队列建设的高质量高效率是国内外流行病学研究者面临的主要挑战。2016年,国家重点研发计划资助的中国国家出生队列(China National Birth Cohort)建设正式启动。该队列在设计实施过程中,不断积累经验,充分运用网络和信息化手段,探索并建立了一套“云端信息平台”,以支撑覆盖全国16家单位的出生队列建设。经过四年的发展,该系统平台在“出生队列人群招募和随访管理、数据的实时交互、队列质量控制、多级权限管理和职能划分”等多个方面已经发展出一整套完善的建设方案。该平台的设计框架和功能要素对于我国今后出生队列乃至大型人群研究的信息化建设具有重要的参考意义。展开更多
Balfour和Stewart于1869年首次报道了门静脉海绵样变(cavernous transformation of the portal vein,CTPV)[1]。门静脉高压患者CTPV的发生率约为5%~10%[2]。CTPV患者常合并消化道出血、顽固性腹水等门脉高压症状。经颈静脉肝内门体分流...Balfour和Stewart于1869年首次报道了门静脉海绵样变(cavernous transformation of the portal vein,CTPV)[1]。门静脉高压患者CTPV的发生率约为5%~10%[2]。CTPV患者常合并消化道出血、顽固性腹水等门脉高压症状。经颈静脉肝内门体分流术(transjugular intrahepatic portosystemic shunt,TIPS)是治疗肝硬化门脉高压引起的顽固性腹水及消化道出血的有效治疗方法[3-5]。展开更多
Aberration-corrected scanning transmission electron microscopy was employed to investigate the microstructures and secondary phases in LaBaCo2O5.5＋δ（LBCO） thin films grown on SrTiO3 （STO） substrates. The as-grow...Aberration-corrected scanning transmission electron microscopy was employed to investigate the microstructures and secondary phases in LaBaCo2O5.5＋δ（LBCO） thin films grown on SrTiO3 （STO） substrates. The as-grown films showed an epitaxial growth on the substrates with atomically sharp interfaces and orientation relationships of [100]LBCO//[100]STO and （001）LBCO//（001）STO. Secondary phases were observed in the films, which strongly depended on the sample fabrication conditions. In the film prepared at a temperature of 900 ℃, nano-scale CoO pillars nucleated on the substrate, and grew along the [001] direction of the film. In the film grown at a temperature of 1000 ℃, isolated nano-scale C0304 particles appeared, which promoted the growth of {111 } twinning structures in the film. The orientation relationships and the interfaces between the secondary phases and the films were illustrated, and the growth mechanism of the film was discussed.展开更多
The extent to which factors affect the probability of clinical pregnancy in the first fresh embryo transfer after assisted conception is unknown.In order to examine the predictors of clinical pregnancy,a retrospective...The extent to which factors affect the probability of clinical pregnancy in the first fresh embryo transfer after assisted conception is unknown.In order to examine the predictors of clinical pregnancy,a retrospective cohort study was launched between January 1,2013 and December 31,2016 in four infertility clinics including 19837 in vitro fertilization and intracytoplasmic sperm injection(IVF/ICSI)fresh cycles with known outcomes and relevant records.A multivariable logistic regression was used to select the most significant predictors in the final nomogram for predicting clinical pregnancy.Furthermore,the model was validated by an independent validation set and the performance of the model was evaluated by the receiver operating characteristic(ROC)curves along with the area under the ROC curve(AUC)and calibration plots.In a training set including 17854 participants,we identified that female age,tubal factor,number of embryos transferred,endometrial thickness and number of good-quality embryos were independent predictors for clinical pregnancy.We developed a nomogram using these five factors and the predictive ability was 0.66 for AUC(95%CI=0.64−0.68),which was independently validated in the validation set(AUC=0.66,95%CI=0.65−0.68).Our results show that some specific factors can be used to provide infertile couples with an accurate assessment of clinical pregnancy following assisted conception and facilitate to guide couples and clinicians.展开更多
The BiOCl(BOC)synthesized by the water bath heating method was treated with sodium borohydride(NaBH_(4))to introduce oxygen vacancies(OVs).At the same time,Au nanoparticles were loaded to prepare a series of Au/BiOCl ...The BiOCl(BOC)synthesized by the water bath heating method was treated with sodium borohydride(NaBH_(4))to introduce oxygen vacancies(OVs).At the same time,Au nanoparticles were loaded to prepare a series of Au/BiOCl samples with different ratios.OVs and Au nanoparticles can promote the light absorption of host photocatalyst in the visible region.The calculated work function of BiOCl and Au can verify the existence of Ohmic contact between the interface of them,which is conducive to the separation of charge carriers.Through a series of photoelectric tests,it was verified experimentally that the separation of charge carriers is indeed enhanced.The high-energy hot electrons produced by Au under the surface plasmon resonance(SPR)effect can increase the counts of electrons to participate in the CO_(2)reduction reaction.Especially for 1.0%-Au/BOC,the yields of CO can reach 43.16μmol g^(−1)h^(−1),which is 6.6 times more than that of BOC.Therefore,loading precious metal on semiconductors is an effective strategy to promote the photocatalytic performance of CO_(2)reduction reactions.展开更多
Anodic aluminum oxide(AAO)with independently controlled period,porosity,and height is used as the model surface to study the single structural parameter effect on breast cancer cell behaviors,including cell polarity a...Anodic aluminum oxide(AAO)with independently controlled period,porosity,and height is used as the model surface to study the single structural parameter effect on breast cancer cell behaviors,including cell polarity and cell viability.It is found that the quantity of multipolar cells and cell viability increases as the nanodent period increases from 100 to 300 nm,while the number of bipolar cells has almost no change until there is a dramatic decrease as the period increases to 3oo nm.After anodizing nanodents into nanopores,the numbers of both bipolar cells and the cell viability increase significantly with the porosity increase.However,as the porosity further increases and the nanopore changes into a nanocone pillar,most of the cells become nonpolar spheres and the cell viability decreases.Increasing the height of the nanocone pillar has little effect on the cell polarity;the cell viability increases slightly with the increase of the nanocone pillar height.These results reveal the influence of individual nanostructure parameters on the cell behavior,especially the cell polarity and the cell viability,which can help to design the surface to make the cell grow as desired.展开更多
文摘CHDTEPDB(URL:http://chdtepdb.com/)is a manually integrated database for congenital heart disease(CHD)that stores the expression profiling data of CHD derived from published papers,aiming to provide rich resources for investigating a deeper correlation between human CHD and aberrant transcriptome expression.The develop-ment of human diseases involves important regulatory roles of RNAs,and expression profiling data can reflect the underlying etiology of inherited diseases.Hence,collecting and compiling expression profiling data is of critical significance for a comprehensive understanding of the mechanisms and functions that underpin genetic diseases.CHDTEPDB stores the expression profiles of over 200 sets of 7 types of CHD and provides users with more convenient basic analytical functions.Due to the differences in clinical indicators such as disease type and unavoidable detection errors among various datasets,users are able to customize their selection of corresponding data for personalized analysis.Moreover,we provide a submission page for researchers to submit their own data so that increasing expression profiles as well as some other histological data could be supplemented to the database.CHDTEPDB is a user-friendly interface that allows users to quickly browse,retrieve,download,and analyze their target samples.CHDTEPDB will significantly improve the current knowledge of expression profiling data in CHD and has the potential to be exploited as an important tool for future research on the disease.
基金supported by the Nanjing Medical Science and Technique Development Foundation(ZKX17041)the Natural Science Foundation of Jiangsu Province(BK20161120)+2 种基金the Maternal and child health research project of Jiangsu Province(F201628)the Priority Academic Program Development of Jiangsu Higher Education Institutions(Public Health and Preventive Medicine)Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(PPZY2015A067)。
文摘Triple negative breast cancer(TNBC) is an aggressive subtype of breast cancer that currently lacks effective biomarkers and therapeutic targets required to investigate the diagnosis and treatment of TNBC. Here we performed a comprehensive differential analysis of 165 TNBC samples by integrating RNA-seq data of breast tumor tissues and adjacent normal tissues from both our cohort and The Cancer Genome Atlas(TCGA). Pathway enrichment analysis was conducted to evaluate the biological function of TNBC-specific expressed genes. Further multivariate Cox proportional hazard regression was performed to evaluate the effect of these genes on TNBC prognosis. In this report, we identified a total of 148 TNBC-specific expressed genes that were primarily enriched in mammary gland morphogenesis and hormone levels related pathways, suggesting that mammary gland morphogenesis might play a unique role in TNBC patients differing from other breast cancer types. Further survival analysis revealed that nine genes(FSIP1, ADCY5, FSD1, HMSD, CMTM5, AFF3, CYP2 A7, ATP1 A2,and C11 orf86) were significantly associated with the prognosis of TNBC patients, while three of them(ADCY5,CYP2 A7, and ATP1 A2) were involved in the hormone-related pathways. These findings indicated the vital role of the hormone-related genes in TNBC tumorigenesis and may provide some independent prognostic markers as well as novel therapeutic targets for TNBC.
基金Science and Technology Program of Shaanxi Province,Grant/Award Number:2017KJXX-16Natural Science Basic。
文摘Platinum telluride(PtTe_(2)),a member of metallic transition metal dichalcogenides,provides a new platform for investigating various properties such as type-II Dirac fermions,topological superconductivity,and wide-band photodetection.However,the study of PtTe_(2)is largely limited to exfoliated flakes,and its direct synthesis remains challenging.Herein,we report the controllable synthesis of highly crystalline 2D PtTe_(2)crystals with tunable morphology and thickness via chemical vapor deposition(CVD)growth on Au substrate.By adjusting Te amount and substrate temperature,anisotropic and isotropic growth modes of PtTe_(2)were realized on the solid and molten Au substrates,respectively.The domain size of PtTe_(2)crystal was achieved up to 30μm,and its thickness can be tuned from 5.6 to 50 nm via controlling the growth time.Furthermore,a metal–PtTe_(2)–metal structural device was fabricated to validate the wide-band terahertz(THz)photodetection from 0.04 to 0.3 THz at room temperature.Owing to the high crystallinity of PtTe_(2)crystal,the photodetector acquires high responsivity(30–250 mA W-1 from 0.12 to 0.3 THz),fast response rate(rise time:7μs,decay time:8μs),and high-quality imaging ability.Our work demonstrates the feasibility for realistic exploitation of high-performing photodetection system at THz band based on the CVDgrown 2D Dirac semimetal materials.
文摘大型出生队列是持续、动态地收集个体生命早期暴露信息,探讨暴露与生命远期健康结局因果关联的一种重要的队列研究类型。但由于其设计复杂、实施难度大,如何保证出生队列建设的高质量高效率是国内外流行病学研究者面临的主要挑战。2016年,国家重点研发计划资助的中国国家出生队列(China National Birth Cohort)建设正式启动。该队列在设计实施过程中,不断积累经验,充分运用网络和信息化手段,探索并建立了一套“云端信息平台”,以支撑覆盖全国16家单位的出生队列建设。经过四年的发展,该系统平台在“出生队列人群招募和随访管理、数据的实时交互、队列质量控制、多级权限管理和职能划分”等多个方面已经发展出一整套完善的建设方案。该平台的设计框架和功能要素对于我国今后出生队列乃至大型人群研究的信息化建设具有重要的参考意义。
文摘Balfour和Stewart于1869年首次报道了门静脉海绵样变(cavernous transformation of the portal vein,CTPV)[1]。门静脉高压患者CTPV的发生率约为5%~10%[2]。CTPV患者常合并消化道出血、顽固性腹水等门脉高压症状。经颈静脉肝内门体分流术(transjugular intrahepatic portosystemic shunt,TIPS)是治疗肝硬化门脉高压引起的顽固性腹水及消化道出血的有效治疗方法[3-5]。
基金financially supported by the National Natural Science Foundation of China (Nos. 51501143, 51202185 and 51390472)the National Basic Research Program of China (No. 2015CB654903)Fundamental Research Funds for the Central Universities, China Postdoctoral Science Foundation (No. 2015M572554)
文摘Aberration-corrected scanning transmission electron microscopy was employed to investigate the microstructures and secondary phases in LaBaCo2O5.5＋δ（LBCO） thin films grown on SrTiO3 （STO） substrates. The as-grown films showed an epitaxial growth on the substrates with atomically sharp interfaces and orientation relationships of [100]LBCO//[100]STO and （001）LBCO//（001）STO. Secondary phases were observed in the films, which strongly depended on the sample fabrication conditions. In the film prepared at a temperature of 900 ℃, nano-scale CoO pillars nucleated on the substrate, and grew along the [001] direction of the film. In the film grown at a temperature of 1000 ℃, isolated nano-scale C0304 particles appeared, which promoted the growth of {111 } twinning structures in the film. The orientation relationships and the interfaces between the secondary phases and the films were illustrated, and the growth mechanism of the film was discussed.
文摘The extent to which factors affect the probability of clinical pregnancy in the first fresh embryo transfer after assisted conception is unknown.In order to examine the predictors of clinical pregnancy,a retrospective cohort study was launched between January 1,2013 and December 31,2016 in four infertility clinics including 19837 in vitro fertilization and intracytoplasmic sperm injection(IVF/ICSI)fresh cycles with known outcomes and relevant records.A multivariable logistic regression was used to select the most significant predictors in the final nomogram for predicting clinical pregnancy.Furthermore,the model was validated by an independent validation set and the performance of the model was evaluated by the receiver operating characteristic(ROC)curves along with the area under the ROC curve(AUC)and calibration plots.In a training set including 17854 participants,we identified that female age,tubal factor,number of embryos transferred,endometrial thickness and number of good-quality embryos were independent predictors for clinical pregnancy.We developed a nomogram using these five factors and the predictive ability was 0.66 for AUC(95%CI=0.64−0.68),which was independently validated in the validation set(AUC=0.66,95%CI=0.65−0.68).Our results show that some specific factors can be used to provide infertile couples with an accurate assessment of clinical pregnancy following assisted conception and facilitate to guide couples and clinicians.
基金supported by the National Natural Science Foundation of China(Nos.51772183.52072230)the Yulin Science and Technology Project(No.CXY-2020-040)。
文摘The BiOCl(BOC)synthesized by the water bath heating method was treated with sodium borohydride(NaBH_(4))to introduce oxygen vacancies(OVs).At the same time,Au nanoparticles were loaded to prepare a series of Au/BiOCl samples with different ratios.OVs and Au nanoparticles can promote the light absorption of host photocatalyst in the visible region.The calculated work function of BiOCl and Au can verify the existence of Ohmic contact between the interface of them,which is conducive to the separation of charge carriers.Through a series of photoelectric tests,it was verified experimentally that the separation of charge carriers is indeed enhanced.The high-energy hot electrons produced by Au under the surface plasmon resonance(SPR)effect can increase the counts of electrons to participate in the CO_(2)reduction reaction.Especially for 1.0%-Au/BOC,the yields of CO can reach 43.16μmol g^(−1)h^(−1),which is 6.6 times more than that of BOC.Therefore,loading precious metal on semiconductors is an effective strategy to promote the photocatalytic performance of CO_(2)reduction reactions.
基金supported by the National Natural Science Foundation of China(Grant No.21965030)the Natural Science Foundation of Shaanxi Province(Grant Nos.2020JM-287 and 2020SF-190)the Fundamental Research Funds for the Central Universities(Grant Nos.GK202003015 and 2017TS015)。
文摘Anodic aluminum oxide(AAO)with independently controlled period,porosity,and height is used as the model surface to study the single structural parameter effect on breast cancer cell behaviors,including cell polarity and cell viability.It is found that the quantity of multipolar cells and cell viability increases as the nanodent period increases from 100 to 300 nm,while the number of bipolar cells has almost no change until there is a dramatic decrease as the period increases to 3oo nm.After anodizing nanodents into nanopores,the numbers of both bipolar cells and the cell viability increase significantly with the porosity increase.However,as the porosity further increases and the nanopore changes into a nanocone pillar,most of the cells become nonpolar spheres and the cell viability decreases.Increasing the height of the nanocone pillar has little effect on the cell polarity;the cell viability increases slightly with the increase of the nanocone pillar height.These results reveal the influence of individual nanostructure parameters on the cell behavior,especially the cell polarity and the cell viability,which can help to design the surface to make the cell grow as desired.
