Background To evaluate the association between coexisting intracranial and extracranial carotid artery atherosclerotic diseases and ipsilateral acute cerebral infarct(ACI)in symptomatic patients by using magnetic reso...Background To evaluate the association between coexisting intracranial and extracranial carotid artery atherosclerotic diseases and ipsilateral acute cerebral infarct(ACI)in symptomatic patients by using magnetic resonance(MR)vessel wall imaging.Methods Symptomatic patients were recruited from a cross-sectional,multicentre study of Chinese Atherosclerosis Risk Evaluation(CARE-II).All patients underwent MR imaging for extracranial carotid arterial wall,intracranial artery and brain.Coexisting intracranial stenosis≥50%and extracranial carotid artery mean wall thickness(MWT)≥1 mm and plaque compositions at the same side were evaluated and the ipsilateral ACI was identified.The association between coexisting atherosclerotic diseases and ACI was evaluated using logistic regression.Results 351 patients were recruited.Patients with ipsilateral ACI had significantly greater prevalence of coexisting intracranial stenosis≥50%and carotid MWT≥1 mm(20.5%vs 4.9%,p<0.001),calcification(15.1%vs 4.4%,p=0.001)and lipid-rich necrotic core(LRNC)(19.2%vs 7.8%,p=0.002)compared with those without.Coexisting intracranial artery stenosis≥50%and carotid MWT≥1 mm(OR 5.043,95%CI 2.378 to 10.694;p<0.001),calcification(OR 3.864,95%CI 1.723 to 8.664;p=0.001)and LRNC(OR 2.803,95%CI 1.455 to 5.401;p=0.002)were significantly associated with ipsilateral ACI.After adjusting for confounding factors,the aforementioned associations remained statistically significant(intracranial stenosis≥50%coexisting with carotid MWT≥1 mm:OR 4.313,95%CI 1.937 to 9.601,p<0.001;calcification:OR 3.606,95%CI 1.513 to 8.593,p=0.004;LRNC:OR 2.358,95%CI 1.166 to 4.769,p=0.017).Conclusions Coexistence of intracranial artery severe stenosis and extracranial carotid artery large burden and intraplaque components of calcification and LRNC are independently associated with ipsilateral ACI.Trial registration number https://www.clinicaltrials.gov/.Unique identifier:NCT02017756.展开更多
Microstructure,mechanical and corrosion properties of as-cast Mg-2Dy-xZn(x=0,0.1,0.5,and 1)(at.%)alloys were investigated.The microstructures of the as-cast Mg-2Dy and Mg-2Dy-0.1Zn alloys mainly consisted ofα-Mg phas...Microstructure,mechanical and corrosion properties of as-cast Mg-2Dy-xZn(x=0,0.1,0.5,and 1)(at.%)alloys were investigated.The microstructures of the as-cast Mg-2Dy and Mg-2Dy-0.1Zn alloys mainly consisted ofα-Mg phase and Mg_(24)Dy_(5)eutectic phase.With 0.5 at.%Zn addition,Mg_(12)ZnDy phase with 18R-type long period stacking ordered(LPSO)structure and Mg_(2)Dy phase precipitated at the grain boundaries.When the content of Zn is 1 at.%,only the Mg_(3)Zn_(3)Dy_(2)phase formed in theα-Mg matrix.The electrochemical measurements and immersion testing results indicated that the Mg-2Dy-0.1Zn alloy exhibited the best corrosion resistance.It revealed that the morphology,scale,amount and distribution of the second phase have a great effect on the corrosion resistance of alloy.Additionally,the tensile testing results showed that the Mg-2Dy-0.5Zn alloy exhibited the higher tensile strength and good elongation,especially at 200℃.The improvement of mechanical properties was mainly due to the strengthening of LPSO phase and grain refinement of α-Mg.展开更多
目的评估Dynesys动态内固定联合减压治疗腰椎退行性疾病的中长期临床疗效。方法回顾性分析2008年3月至2015年3月采用Dynesys动态内固定联合减压治疗145例腰椎退行性疾病患者,男84例,女61例;年龄(55.9±7.1)岁(范围19~75岁),其中腰...目的评估Dynesys动态内固定联合减压治疗腰椎退行性疾病的中长期临床疗效。方法回顾性分析2008年3月至2015年3月采用Dynesys动态内固定联合减压治疗145例腰椎退行性疾病患者,男84例,女61例;年龄(55.9±7.1)岁(范围19~75岁),其中腰椎间盘突出症69例、退行性腰椎管狭窄症53例、Ⅰ度退行性腰椎滑脱症23例。采用视觉模拟评分(visual analogue scale,VAS)、日本骨科学会(Japanese Orthopaedic Association Scores,JOA)腰痛评分(29分)和Oswestry功能障碍指数(Oswestry disability index,ODI)评估手术前后临床症状情况;腰椎侧位X线片测量手术节段与相邻节段椎间隙高度;腰椎动力位X线片测量手术节段与相邻节段椎间活动度(range of motion,ROM);椎间盘Pfirrmann分级系统评估手术节段与邻近节段椎间盘退变情况。结果腰椎间盘突出症患者腰背部和下肢VAS评分、ODI、腰椎JOA评分分别由术前(6.6±1.7)分、(7.1±1.4)分、63.1%±10.2%、(12.5±2.4)分,改善至末次随访时的(2.6±1.0)分、(2.8±0.9)分、30.9%±9.8%、(22.4±2.1)分,差异均有统计学意义。腰椎椎管狭窄症患者腰背部和下肢VAS评分、ODI和腰椎JOA评分分别由术前(6.3±2.2)分、(6.9±1.3)分、63.4%±8.5%、(12.8±2.7)分,改善至末次随访时的(2.4±1.2)分、(2.8±1.0)分、35.1%±12.0%、(22.2±2.2)分,差异均有统计学意义。Ⅰ度退行性腰椎滑脱症患者腰背部和下肢VAS评分、ODI、腰椎JOA评分分别由术前(5.7±2.3)分、(6.7±0.9)分、65.7%±10.0%、(12.5±2.7)分,改善至末次随访时的(2.2±1.2)分、(2.7±1.1)分、37.0%±11.8%、(22.4±2.6)分,差异均有统计学意义。术后1年和末次随访时,手术节段和尾侧椎间隙高度与术前相比均降低,但差异无统计学意义。头侧邻近节段,末次随访时椎间隙高度(11.3±1.8)mm较术前(12.1±1.9)mm和术后1年(11.7±1.6)mm相比下降,差异有统计学意义(F=6.46,P=0.001)。手术节段ROM由术前7.6°±2.2°下降至术后1年时展开更多
Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nuc...Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.展开更多
The Macao Science Satellite-1 is a two-satellite constellation specifically designed to study the geomagnetic field and particle radiation environment in low Earth orbit,particularly in the South Atlantic Anomaly regi...The Macao Science Satellite-1 is a two-satellite constellation specifically designed to study the geomagnetic field and particle radiation environment in low Earth orbit,particularly in the South Atlantic Anomaly region,with a low inclination orbit.Each of the two MSS-1 satellites carries a medium-energy electron spectrometer(MES).The MES sensor heads are based on pinhole imaging technology,which can simultaneously measure 50-600 keV electrons incident from nine directions with a field of view(FOV)of 180°×30°.The two MESs can realize the pitch angle coverage of medium energy electrons at most positions in the orbit.The MSS-1 A/B MESs can realize direct observation of precipitating electrons and electrons near their loss cones.It can help to study the electron generation mechanism in the inner radiation belt and quantify the precipitation of magnetospheric energetic electrons.Combined with the geomagnetic index,solar wind parameters,interplanetary magnetic field conditions,etc.,it can also help to build a dynamic evolution model of energetic electrons in the near-Earth space,to realize the early warning and prediction of space weather based on the observation data,which can provide safety for spacecraft and astronauts in the nearEarth space.展开更多
Although transvaginal mesh(TVM)repair is no longer used in some countries,long-term outcomes after TVM surgery are of great importance globally.However,reports with follow-up>10 years are limited.Thus,this study ai...Although transvaginal mesh(TVM)repair is no longer used in some countries,long-term outcomes after TVM surgery are of great importance globally.However,reports with follow-up>10 years are limited.Thus,this study aimed to report outcomes in a prospective cohort with at least 10 years of follow-up.Women with stageⅢ–Ⅳsymptomatic prolapse were approached consecutively from 2008 to 2013 at one tertiary hospital.The main outcome measure was symptomatic failure.Secondary outcomes included anatomic failure,recurrence,patient satisfaction,complications,and reoperation.The Kaplan-Meier curve was used to estimate the cumulative failure rate.Of the 121 patients enrolled in the study,103(85.1%)completed a median follow-up of 11 years.The estimated probability rates of symptomatic and anatomic failure were 17.6%and 8.8%in 11 years,respectively.The estimated incidence of symptomatic failure increased by 8.2%between 5 and 11 years;however,the corresponding rate for anatomic failure was 3.7%.The most common complication was vaginal mesh exposure,and its estimated probability increased from 19.3%to 28.4%from 5 to 11 years,respectively.Office trimming resolved 80.0%of vaginal exposures.These patients did not report decreased overall satisfaction.Patients with vaginal mesh exposure requiring>3 office procedures or mesh removal in the operating room(5.8%by 11 years)had lower satisfaction rates(P<0.01)and were defined as having severe mesh exposure.The rates of postoperative pain,reoperation,and Patient Global Impression of Improvement≥2 were 2.5%,3.3%,and 94.2%,respectively.The results of this study implied that TVM treatment gradually increased the symptomatic failure rate but provided durable anatomical support of the vaginal wall.Vaginal mesh exposure was common in women who were largely not sexually active;however,80%of the cases could be managed in the outpatient clinic,which did not affect patient satisfaction.展开更多
Cold exposure is a pervasive stressor in the polar and subpolar regions,exerting both acute and chronic effects on individuals.This environmental factor is known to induce physiological stress,compromise immune respon...Cold exposure is a pervasive stressor in the polar and subpolar regions,exerting both acute and chronic effects on individuals.This environmental factor is known to induce physiological stress,compromise immune response efficacy,and increase susceptibility to various diseases.Chronic cold exposure,characterized by repetitive nonconsecutive exposure to suboptimal temperatures over an extended duration.展开更多
To the Editor:Pelvic organ prolapse(POP)is a common pelvic floor disorder in older women and is responsible for a significant decrease in health-related quality of life.With the increased longevity of women,POP is bec...To the Editor:Pelvic organ prolapse(POP)is a common pelvic floor disorder in older women and is responsible for a significant decrease in health-related quality of life.With the increased longevity of women,POP is becoming a major health concern.[1]Laparoscopic sacrocolpopexy(LSC)is a common mesh-based surgical approach for severe prolapse,and a high cure rate and few complications have been reported.[2]To our knowledge,the long-term durability,complications,and pelvic floor symptoms after LSC have not been fully evaluated in detail.A few studies assessed the 5-year outcome after LSC,but these reports were limited by their retrospective design,uneven follow-up time,or inconsistent outcome assessment.展开更多
基金This study is funded by the grants of National Key R&D Program of China(No.2017YFC1307900,2017YFC1307904)National Natural Science Foundation of China(81771825)Beijing Municipal Science and Technology Commission(D171100003017003).
文摘Background To evaluate the association between coexisting intracranial and extracranial carotid artery atherosclerotic diseases and ipsilateral acute cerebral infarct(ACI)in symptomatic patients by using magnetic resonance(MR)vessel wall imaging.Methods Symptomatic patients were recruited from a cross-sectional,multicentre study of Chinese Atherosclerosis Risk Evaluation(CARE-II).All patients underwent MR imaging for extracranial carotid arterial wall,intracranial artery and brain.Coexisting intracranial stenosis≥50%and extracranial carotid artery mean wall thickness(MWT)≥1 mm and plaque compositions at the same side were evaluated and the ipsilateral ACI was identified.The association between coexisting atherosclerotic diseases and ACI was evaluated using logistic regression.Results 351 patients were recruited.Patients with ipsilateral ACI had significantly greater prevalence of coexisting intracranial stenosis≥50%and carotid MWT≥1 mm(20.5%vs 4.9%,p<0.001),calcification(15.1%vs 4.4%,p=0.001)and lipid-rich necrotic core(LRNC)(19.2%vs 7.8%,p=0.002)compared with those without.Coexisting intracranial artery stenosis≥50%and carotid MWT≥1 mm(OR 5.043,95%CI 2.378 to 10.694;p<0.001),calcification(OR 3.864,95%CI 1.723 to 8.664;p=0.001)and LRNC(OR 2.803,95%CI 1.455 to 5.401;p=0.002)were significantly associated with ipsilateral ACI.After adjusting for confounding factors,the aforementioned associations remained statistically significant(intracranial stenosis≥50%coexisting with carotid MWT≥1 mm:OR 4.313,95%CI 1.937 to 9.601,p<0.001;calcification:OR 3.606,95%CI 1.513 to 8.593,p=0.004;LRNC:OR 2.358,95%CI 1.166 to 4.769,p=0.017).Conclusions Coexistence of intracranial artery severe stenosis and extracranial carotid artery large burden and intraplaque components of calcification and LRNC are independently associated with ipsilateral ACI.Trial registration number https://www.clinicaltrials.gov/.Unique identifier:NCT02017756.
基金This work was financially supported by National Natural Science Foundation of China(51301082,51301083).
文摘Microstructure,mechanical and corrosion properties of as-cast Mg-2Dy-xZn(x=0,0.1,0.5,and 1)(at.%)alloys were investigated.The microstructures of the as-cast Mg-2Dy and Mg-2Dy-0.1Zn alloys mainly consisted ofα-Mg phase and Mg_(24)Dy_(5)eutectic phase.With 0.5 at.%Zn addition,Mg_(12)ZnDy phase with 18R-type long period stacking ordered(LPSO)structure and Mg_(2)Dy phase precipitated at the grain boundaries.When the content of Zn is 1 at.%,only the Mg_(3)Zn_(3)Dy_(2)phase formed in theα-Mg matrix.The electrochemical measurements and immersion testing results indicated that the Mg-2Dy-0.1Zn alloy exhibited the best corrosion resistance.It revealed that the morphology,scale,amount and distribution of the second phase have a great effect on the corrosion resistance of alloy.Additionally,the tensile testing results showed that the Mg-2Dy-0.5Zn alloy exhibited the higher tensile strength and good elongation,especially at 200℃.The improvement of mechanical properties was mainly due to the strengthening of LPSO phase and grain refinement of α-Mg.
文摘目的评估Dynesys动态内固定联合减压治疗腰椎退行性疾病的中长期临床疗效。方法回顾性分析2008年3月至2015年3月采用Dynesys动态内固定联合减压治疗145例腰椎退行性疾病患者,男84例,女61例;年龄(55.9±7.1)岁(范围19~75岁),其中腰椎间盘突出症69例、退行性腰椎管狭窄症53例、Ⅰ度退行性腰椎滑脱症23例。采用视觉模拟评分(visual analogue scale,VAS)、日本骨科学会(Japanese Orthopaedic Association Scores,JOA)腰痛评分(29分)和Oswestry功能障碍指数(Oswestry disability index,ODI)评估手术前后临床症状情况;腰椎侧位X线片测量手术节段与相邻节段椎间隙高度;腰椎动力位X线片测量手术节段与相邻节段椎间活动度(range of motion,ROM);椎间盘Pfirrmann分级系统评估手术节段与邻近节段椎间盘退变情况。结果腰椎间盘突出症患者腰背部和下肢VAS评分、ODI、腰椎JOA评分分别由术前(6.6±1.7)分、(7.1±1.4)分、63.1%±10.2%、(12.5±2.4)分,改善至末次随访时的(2.6±1.0)分、(2.8±0.9)分、30.9%±9.8%、(22.4±2.1)分,差异均有统计学意义。腰椎椎管狭窄症患者腰背部和下肢VAS评分、ODI和腰椎JOA评分分别由术前(6.3±2.2)分、(6.9±1.3)分、63.4%±8.5%、(12.8±2.7)分,改善至末次随访时的(2.4±1.2)分、(2.8±1.0)分、35.1%±12.0%、(22.2±2.2)分,差异均有统计学意义。Ⅰ度退行性腰椎滑脱症患者腰背部和下肢VAS评分、ODI、腰椎JOA评分分别由术前(5.7±2.3)分、(6.7±0.9)分、65.7%±10.0%、(12.5±2.7)分,改善至末次随访时的(2.2±1.2)分、(2.7±1.1)分、37.0%±11.8%、(22.4±2.6)分,差异均有统计学意义。术后1年和末次随访时,手术节段和尾侧椎间隙高度与术前相比均降低,但差异无统计学意义。头侧邻近节段,末次随访时椎间隙高度(11.3±1.8)mm较术前(12.1±1.9)mm和术后1年(11.7±1.6)mm相比下降,差异有统计学意义(F=6.46,P=0.001)。手术节段ROM由术前7.6°±2.2°下降至术后1年时
文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.
基金supported by the National Natural Science Foundation of China(Grant No.42274225)the Science and Technology Development Fund,Macao SAR(Grant No.SKL-LPS(MUST)-2024-2026)。
文摘The Macao Science Satellite-1 is a two-satellite constellation specifically designed to study the geomagnetic field and particle radiation environment in low Earth orbit,particularly in the South Atlantic Anomaly region,with a low inclination orbit.Each of the two MSS-1 satellites carries a medium-energy electron spectrometer(MES).The MES sensor heads are based on pinhole imaging technology,which can simultaneously measure 50-600 keV electrons incident from nine directions with a field of view(FOV)of 180°×30°.The two MESs can realize the pitch angle coverage of medium energy electrons at most positions in the orbit.The MSS-1 A/B MESs can realize direct observation of precipitating electrons and electrons near their loss cones.It can help to study the electron generation mechanism in the inner radiation belt and quantify the precipitation of magnetospheric energetic electrons.Combined with the geomagnetic index,solar wind parameters,interplanetary magnetic field conditions,etc.,it can also help to build a dynamic evolution model of energetic electrons in the near-Earth space,to realize the early warning and prediction of space weather based on the observation data,which can provide safety for spacecraft and astronauts in the nearEarth space.
基金supported by the National Natural Science Foundation of China(81830043,81771561)the National High Level Hospital Clinical Research Funding(2022-PUMCH-A-113,2022-PUMCH-C-031)+1 种基金the National Key Research and Development Program of China(2018YFC2002201)the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2020-PT320-003)。
文摘Although transvaginal mesh(TVM)repair is no longer used in some countries,long-term outcomes after TVM surgery are of great importance globally.However,reports with follow-up>10 years are limited.Thus,this study aimed to report outcomes in a prospective cohort with at least 10 years of follow-up.Women with stageⅢ–Ⅳsymptomatic prolapse were approached consecutively from 2008 to 2013 at one tertiary hospital.The main outcome measure was symptomatic failure.Secondary outcomes included anatomic failure,recurrence,patient satisfaction,complications,and reoperation.The Kaplan-Meier curve was used to estimate the cumulative failure rate.Of the 121 patients enrolled in the study,103(85.1%)completed a median follow-up of 11 years.The estimated probability rates of symptomatic and anatomic failure were 17.6%and 8.8%in 11 years,respectively.The estimated incidence of symptomatic failure increased by 8.2%between 5 and 11 years;however,the corresponding rate for anatomic failure was 3.7%.The most common complication was vaginal mesh exposure,and its estimated probability increased from 19.3%to 28.4%from 5 to 11 years,respectively.Office trimming resolved 80.0%of vaginal exposures.These patients did not report decreased overall satisfaction.Patients with vaginal mesh exposure requiring>3 office procedures or mesh removal in the operating room(5.8%by 11 years)had lower satisfaction rates(P<0.01)and were defined as having severe mesh exposure.The rates of postoperative pain,reoperation,and Patient Global Impression of Improvement≥2 were 2.5%,3.3%,and 94.2%,respectively.The results of this study implied that TVM treatment gradually increased the symptomatic failure rate but provided durable anatomical support of the vaginal wall.Vaginal mesh exposure was common in women who were largely not sexually active;however,80%of the cases could be managed in the outpatient clinic,which did not affect patient satisfaction.
基金funded by the National Natural Science Foundation of China(Grant No.32172817)the Natural Science Foundation of Heilongjiang Province Grant(Grant No.LH2022C071)the Heilongjiang Bayi Agricultural University for San Heng San Zong(Grant No.ZRCQC202003).
文摘Cold exposure is a pervasive stressor in the polar and subpolar regions,exerting both acute and chronic effects on individuals.This environmental factor is known to induce physiological stress,compromise immune response efficacy,and increase susceptibility to various diseases.Chronic cold exposure,characterized by repetitive nonconsecutive exposure to suboptimal temperatures over an extended duration.
基金supported by grants from the National Natural Science Foundation of China(No.81830043)the Fundamental Research Funds for the Central Universities(No.3332022118)+1 种基金the National Key R&D Program of China(Nos.2021YFC2701300 and 2021YFC2701302)the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(No.2021-PT320-001)
文摘To the Editor:Pelvic organ prolapse(POP)is a common pelvic floor disorder in older women and is responsible for a significant decrease in health-related quality of life.With the increased longevity of women,POP is becoming a major health concern.[1]Laparoscopic sacrocolpopexy(LSC)is a common mesh-based surgical approach for severe prolapse,and a high cure rate and few complications have been reported.[2]To our knowledge,the long-term durability,complications,and pelvic floor symptoms after LSC have not been fully evaluated in detail.A few studies assessed the 5-year outcome after LSC,but these reports were limited by their retrospective design,uneven follow-up time,or inconsistent outcome assessment.
