This paper introduces the principle of space vector pulse width modulation （SVPWM）, and discusses a method for implementing the SVPWM based on MATLAB/SIMULINK, as well as modeling of AC servo system with permanent m...This paper introduces the principle of space vector pulse width modulation （SVPWM）, and discusses a method for implementing the SVPWM based on MATLAB/SIMULINK, as well as modeling of AC servo system with permanent magnet synchronous motor （PMSM）. Simulation results show that the model is effective, and the method provides a frame of reference for software and hardware designs which can be applied in high temperature superconducting （HTS） flywheel energy storage system （FESS） and linear motor （LM）.展开更多
[目的]探讨化疗药物诱导肝癌细胞凋亡的潜在机制。[方法]高通量测序检测顺铂处理后肝癌细胞HepG2的失调miRNA。过表达失调miRNA后,检测HepG2的凋亡水平。miRDB在线分析和RNAi筛选鉴定miRNA的潜在底物。[结果]顺铂处理后,HepG2的凋亡水...[目的]探讨化疗药物诱导肝癌细胞凋亡的潜在机制。[方法]高通量测序检测顺铂处理后肝癌细胞HepG2的失调miRNA。过表达失调miRNA后,检测HepG2的凋亡水平。miRDB在线分析和RNAi筛选鉴定miRNA的潜在底物。[结果]顺铂处理后,HepG2的凋亡水平上升(18.23±3.01 vs 74.97±11.25,t=8.564,P<0.05)。过表达miR-301a-3p时,HepG2的凋亡水平上升(16.40±7.97 vs 56.12±9.37,t=5.590,P<0.05)。敲低miR-301a-3p时,HepG2的凋亡水平下降(21.34±3.34 vs 6.78±1.11,t=7.165,P<0.05)。敲低MDM4时,HepG2的凋亡水平上升(16.80±7.88 vs 72.85±10.70,t=7.302,P<0.05)。过表达MDM4时,HepG2的凋亡水平下降(19.11±5.02 vs 6.04±1.02,t=4.432,P<0.05)。过表达miR-301a-3p时,MDM4的mRNA水平和蛋白水平均下降(0.58±0.04 vs 0.11±0.01,t=19.74,P<0.05);而敲低miR-301a-3p时,MDM4的mRNA水平和蛋白水平均上升(0.58±0.04 vs 1.11±0.23,t=3.932,P<0.05)。肝癌组织中miR-301a-3p的表达水平显著低于癌旁组织(0.65±0.25 vs 0.40±0.04,t=5.949,P<0.05)。肝癌组织中MDM4的表达水平显著高于癌旁组织(0.036±0.025 vs 0.062±0.003,t=6.550,P<0.05)。同时,肝癌组织中miR-301a-3p和MDM4的表达水平呈现负相关(ρ=-0.385,P<0.05)。[结论]顺铂诱导肝癌细胞miR-301a-3p表达上调,miR-301a-3p通过靶向MDM4 mRNA的3’端非编码区,降解了MDM4 mRNA,减少了MDM4的蛋白表达,最终促进了肝癌细胞的凋亡。展开更多
This paper introduces the relevant parameters and related characteristics of the LabVIEW and PCI6221 data acquisition （DAQ） card, describes in detail the approach of building the measure and control platform of virt...This paper introduces the relevant parameters and related characteristics of the LabVIEW and PCI6221 data acquisition （DAQ） card, describes in detail the approach of building the measure and control platform of virtual instrument （VI） using LabVIEW and PCI6221, specifically discusses the system＇s application in high temperature superconductor （HTS） research including the test of HTS volt-ampere characteristics and the HTS magnetic energy storage. The experiments prove that the VI test and control system is easy to build and convenient to use.展开更多
A permanent magnet linear synchronous motor （PMLSM） for a high temperature superconducting （HTS） maglev system has been studied, including the motor structure, control strategy, and analysis techniques. Finite ele...A permanent magnet linear synchronous motor （PMLSM） for a high temperature superconducting （HTS） maglev system has been studied, including the motor structure, control strategy, and analysis techniques. Finite element analysis （FEA） of magnetic field is conducted to accurately calculate major motor parameters. Equivalent electrical circuit is used to predict the drive＇s steady-state characteristics, and a phase variable model is applied to predict the dynamic performance. Preliminary experiment with a prototype has been made to verify the theoretical analysis and the HTS-PM synchronous driving technology.展开更多
基金This workis supported by the Chinese High Technology Development Plan Projectunder Grant No. 2007AA03Z208.
文摘This paper introduces the principle of space vector pulse width modulation （SVPWM）, and discusses a method for implementing the SVPWM based on MATLAB/SIMULINK, as well as modeling of AC servo system with permanent magnet synchronous motor （PMSM）. Simulation results show that the model is effective, and the method provides a frame of reference for software and hardware designs which can be applied in high temperature superconducting （HTS） flywheel energy storage system （FESS） and linear motor （LM）.
文摘[目的]探讨化疗药物诱导肝癌细胞凋亡的潜在机制。[方法]高通量测序检测顺铂处理后肝癌细胞HepG2的失调miRNA。过表达失调miRNA后,检测HepG2的凋亡水平。miRDB在线分析和RNAi筛选鉴定miRNA的潜在底物。[结果]顺铂处理后,HepG2的凋亡水平上升(18.23±3.01 vs 74.97±11.25,t=8.564,P<0.05)。过表达miR-301a-3p时,HepG2的凋亡水平上升(16.40±7.97 vs 56.12±9.37,t=5.590,P<0.05)。敲低miR-301a-3p时,HepG2的凋亡水平下降(21.34±3.34 vs 6.78±1.11,t=7.165,P<0.05)。敲低MDM4时,HepG2的凋亡水平上升(16.80±7.88 vs 72.85±10.70,t=7.302,P<0.05)。过表达MDM4时,HepG2的凋亡水平下降(19.11±5.02 vs 6.04±1.02,t=4.432,P<0.05)。过表达miR-301a-3p时,MDM4的mRNA水平和蛋白水平均下降(0.58±0.04 vs 0.11±0.01,t=19.74,P<0.05);而敲低miR-301a-3p时,MDM4的mRNA水平和蛋白水平均上升(0.58±0.04 vs 1.11±0.23,t=3.932,P<0.05)。肝癌组织中miR-301a-3p的表达水平显著低于癌旁组织(0.65±0.25 vs 0.40±0.04,t=5.949,P<0.05)。肝癌组织中MDM4的表达水平显著高于癌旁组织(0.036±0.025 vs 0.062±0.003,t=6.550,P<0.05)。同时,肝癌组织中miR-301a-3p和MDM4的表达水平呈现负相关(ρ=-0.385,P<0.05)。[结论]顺铂诱导肝癌细胞miR-301a-3p表达上调,miR-301a-3p通过靶向MDM4 mRNA的3’端非编码区,降解了MDM4 mRNA,减少了MDM4的蛋白表达,最终促进了肝癌细胞的凋亡。
文摘This paper introduces the relevant parameters and related characteristics of the LabVIEW and PCI6221 data acquisition （DAQ） card, describes in detail the approach of building the measure and control platform of virtual instrument （VI） using LabVIEW and PCI6221, specifically discusses the system＇s application in high temperature superconductor （HTS） research including the test of HTS volt-ampere characteristics and the HTS magnetic energy storage. The experiments prove that the VI test and control system is easy to build and convenient to use.
文摘A permanent magnet linear synchronous motor （PMLSM） for a high temperature superconducting （HTS） maglev system has been studied, including the motor structure, control strategy, and analysis techniques. Finite element analysis （FEA） of magnetic field is conducted to accurately calculate major motor parameters. Equivalent electrical circuit is used to predict the drive＇s steady-state characteristics, and a phase variable model is applied to predict the dynamic performance. Preliminary experiment with a prototype has been made to verify the theoretical analysis and the HTS-PM synchronous driving technology.
